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Identification of proteins of human colorectal carcinoma cell line SW480 by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

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World Journal of Gastroenterology 2005年第30期11卷 4679-4684页

作者： Ying-Tao Zhang Yi-Ping Geng Le Zhou Bao-Chang Lai lv-sheng si Yi-Li Wang The-Key Laboratory of Biomedical Information Engineering of Education Ministry Institute of Cancer Research School of Life Science and Technology Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China Department of Gastroenterology First Hospital Xi'an Jiaotong University Xi'an 710061 Shaanxi Province China

AIM： To conduct bhe proteomic analysis of human colorectal carcinoma cell line, SW480 by using two-dimensional electrophoresis （2-DE） and matrix-assisted laser desorption /ionization-time of flight mass spectrometry （MALDITOFMS）. METHODS： The total proteins of human colorectal carcinoma cell line, SW480 were separated with 2-DE by using immobilized pH gradient strips and visualized by staining with silver nitrate. The gel images were acquired by scanner and 2-DE analysis software, Image Master 2D Elite. Nineteen distinct protein spots were excised from gel randomly and digested in gel by TPCK-trypsin. Mass analysis of the byptic digest peptides mixture was performed by using MALDI-TOF MS. Peptide mass fingerprints （PMFs） obtained by the MALDI-TOF analysis were used to search NCBI, SWISS-PROT and MSDB databases by using Mascot software. RESULTS： PMF maps of all spots were obtained by MALDI-TOF MS and thirteen proteins were preliminarily identified. CONCLUsiON： The methods of analysis and identification of protein spots of tumor cells in 2-DE gel with silver staining by MALDI-TOF MS derived PMF have been established. Protein expression profile of SW480 has been obtained. It is demonstrated that a combination of proteomics and cell culture is a useful approach to comprehend the process of colon carcinogenesis.

关键词： Colorectal carcinoma SW480 cell line Twodimensional electrophoresis MALDI-TOF MS Peptide mass fingerprinting

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Co-production of hydrogen, oxygen, and electricity via an integrated solar-driven system with decoupled water electrolyzer and Na-Zn ion battery

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Journal of Energy Chemistry 2025年第1期100卷 621-627页

作者： Fei lv Longjie Liu Jiazhe Wu Pengfei Wang Lixia Pan Dengwei Jing Yubin Chen International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University

Combining water electrolysis and rechargeable battery technologies into a single system holds great promise for the co-production of hydrogen （H2） and ***,the design and development of such systems is still in its ***,an integrated hydrogen-oxygen （O2）-electricity co-production system featuring a bipolar membrane-assisted decoupled electrolyzer and a Na-Zn ion battery was established with sodium nickelhexacyanoferrate （NaNiHCF） and Zn2+/Zn as dual redox *** decoupled electrolyzer enables to produce H2and O2in different time and space with almost 100%Faradaic efficiency at 100 mA ***,the charged NaNiHCF and Zn electrodes after the electrolysis processes formed a Na-Zn ion battery,which can generate electricity with an average cell voltage of 1.75 V at 10 m A *** connecting si photovoltaics with the modular electrochemical device,a well-matched solar driven system was built to convert the intermittent solar energy into hydrogen and electric energy with a solar to hydrogen-electricity efficiency of 16.7%,demonstrating the flexible storage and conversion of renewables.

关键词： Hydrogen Electricity Decoupled water electrolyzer Na-Zn ion battery Solar energy

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Challenges in the management of visual and tactile hallucinations in elderly people

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World Journal of Psychiatry 2025年第1期15卷 185-188页

作者： si-sheng Huang Division of Geriatric Psychiatry Department of PsychiatryChanghua Christian HospitalChanghua 500Taiwan Department of Post-Baccalaureate Medicine College of MedicineNational Chung Hsing UniversityTaichung 402Taiwan

This letter provides a concise review of the pertinent literature on visual and tactile hallucinations in elderly *** discussion addresses differential diagnoses and potential underlying mechanisms,as well as the psychopathology associated with tactile hallucinations,and emphasizes the necessity for invest-igation into the possibility of coexisting delusional infestation(parasitosis).These symptoms frequently manifest in patients with primary psychotic disorders,organic mental disorders,and substance use *** proposed pathophy-siological mechanisms may involve dopaminergic imbalances and dysfunction of the striatal dopamine transporter.

关键词： Delusional infestation Dementia Haptic hallucination Scratching behavior Tactile hallucination Visual hallucination Alzheimer’s disease

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重污染企业ESG表现对绿色创新的影响

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价值工程 2025年第1期44卷 74-77页

作者：吕思琦安徽财经大学蚌埠233000

本研究通过对2009年至2021年沪深A股中的重污染企业进行实证分析,深入探讨了企业ESG表现对其实现绿色创新的影响力及其作用机制。研究发现:重污染企业良好的ESG表现能显著提升绿色创新能力;机制分析发现ESG表现可以通过缓解企业融资约... 详细信息

本研究通过对2009年至2021年沪深A股中的重污染企业进行实证分析,深入探讨了企业ESG表现对其实现绿色创新的影响力及其作用机制。研究发现:重污染企业良好的ESG表现能显著提升绿色创新能力;机制分析发现ESG表现可以通过缓解企业融资约束增强企业的绿色创新能力。文章研究结论为我国重污染企业提高ESG表现,加强绿色创新能力有一定启示作用。

关键词： ESG表现绿色创新融资约束

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Comparison of low-density lipoprotein cholesterol/high-density lipoprotein cholesterol and total cholesterol/high-density lipoprotein cholesterol for the prediction of thin-cap fibroatheroma determined by intravascular optical coherence tomography

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Journal of Geriatric Cardiology 2020年第11期17卷 666-673页

作者： Yao WANG si-si ZHANG Qing-Bo lv Ya LI Jin ZHAO Jia HAN Guo-sheng FU Wen-Bin ZHANG Department of Cardiology Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang ProvinceSir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhouChina Department of Infection Control Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhouChina

Background The correlation among the ratios of low-density lipoprotein cholesterol/high-density lipoprotein cholesterol(LDL-C/HDL-C), total cholesterol/high-density lipoprotein cholesterol(TC/HDL-C) and thin-cap fibroatheroma has not yet been established. Methods It was a single center, retrospective observational study. In total, we recruited 421 patients(82.4% men;mean age 65.73 ± 10.44 years) with one culprit vessel which determined by intravascular optical coherence tomography(OCT). The thinnest-capped fibroatheroma(TCFA) group was defined as lipid contents in > 2 quadrants, with the thinnest fibrous cap measuring less than 65 μm. Univariate and multivariate logistic regression were carried out to explore the relationship between lipoprotein ratios, TCFA and other characteristics of plaque. To compare different ratios, the area under curve(AUC) of receiver-operating characteristic(ROC) curve was assessed. Results OCT was performed in 421 patients(TCFA group(n = 109), non-TCFA group(n = 312)). LDL-C/HDL-C in the TCFA group was significantly higher than in the non-TCFA group(2.95 ± 1.20 vs. 2.43 ± 0.92, P sidered independent factors for the prediction of TCFA according to the logistic regression. Based on the AUC comparison, LDL-C/HDL-C and TC/HDL-C had no significant difference statistically(LDL-C/HDL-C AUC: 0.63;TC/HDL-C AUC: 0.61;P = 0.10) for the prediction of TCFA. Conclusions LDL-C/HDL-C and TC/HDL-C could be the independent factors for predicting the presence of TCFA, indicating coronary plaque vulnerability in CAD patients. Moreover, TC/HDL-C also showed a comparative performance for the prediction of TCFA as LDL-C/HDL-C.

关键词： Lipoproteins Optical coherence tomography Total cholesterol

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Superiority:China Mobile in the competition

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The Journal of China Universities of Posts and Telecommunications 2007年第3期14卷 117-122页

作者： FU si-bao lv Ting-jie School of Economies and Management Beijing University of Posts andTelecommunications Beijing 100876 China

The market share between China Mobile and China Unicom has stabilized since 2002. It is found that China Mobile has the superiority in the competition, for example, the scissors movement between its revenue and cost indicates that it has a strong profit generating ability and there is enough room for it to reduce the price. The ratio between its price （marginal income） and marginal cost indicates that there is a very distant limit for it to reduce the price. Its demand is obviously flexible with the price, but it does not use the price weapon abundantly. The reason for the stabilization of the market is that China Mobile withdrew from the competition.

关键词： China Mobile superiority scissor movement between the revenue and cost convergent of the ratio between price and marginal cost elasticity restraint.

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RMAC： A Reliable MAC Protocol Supporting Multicast for Wireless Ad Hoc Networks

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Journal of Computer Science & Technology 2005年第5期20卷 702-712页

作者： Wei-sheng si Cheng-Zhi Li School of Software Dalian University of Technology P.R. China Department of Computer Science and Engineering University of Texas at Arlington U.S.A.

This paper presents a new reliable MAC protocol called “RMAC”supporting reliable multicast for wireless ad hoc networks. By utilizing the busy tones to realize the multicast reliability, RMAC has three novelties：（1） it uses a variablelength control frame to stipulate an order for the receivers to respond, thus solving the feedback collision problem; （2） it extends the usage of busy tone for preventing data frame collisions into the multicast scenario; and （3） it introduces a new usage of busy tone for acknowledging data frames positively. In addition, RMAC is generalized into a comprehensive MAC protocol that provides both reliable and unreliable services for all the three modes of communications： unicast, multicast, and broadcast, making it capable of supporting various upper-layer protocols. The evaluation shows that RMAC achieves high reliability with very limited overhead. RMAC is also compared with other reliable MAC protocols, showing that RMAC not only provides higher reliability but also involves lower cost.

关键词： wireless ad hoc network medium access control reliable multicast busy tone

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Substrate bias effects in p-channel GaN-on-si transistors

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Science China(Information Sciences) 2025年第1期68卷 404-405页

作者： Mengyao ZHAO Jie MA Lanlan YANG Chuanqi PAN Denggui WANG Jianjun ZHOU sheng LI Jiaxing WEI Long ZHANG siyang LIU Weifeng SUN National AsiC System Engineering Research Center School of Integrated Circuits Southeast University National Center of Technology Innovation for Electronic Design Automation Southeast University National Key Laboratory of Solid-State Microwave Devices and Circuits

The drain current （ID） of the n-channel GaN field-effecttransistor （n-FET） is influenced by the substrate-to-source bias voltage (VBS)（substrate bias effect）[1].Actually,nonzero VBsis also likely to occur in p-FET in real application,because the source electrode of p-FET is usually connected to high *** study reports the dependency between IDand VBsin p-FET for the first time,and analyzes the underlying mechanisms from the perspective of vertical electric field （EF） distribution.

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A fast automatic recognition and location algorithm for fetal genital organs in ultrasound images

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第9期10卷 648-658页

作者： sheng TANG si-ping CHEN Post-Doctoral Research Station Shenzhen University Shenzhen 518060 China post-Doctoral WorkStation Microprofit Electronie Co. Ltd Shenzhen 518057 China Department of Biomedical Engineering Shenzhen University Shenzhen 518060 China

Severe sex ratio imbalance at birth is now becoming an important issue in several Asian countries. Its leading immediate cause is prenatal sex-selective abortion following illegal sex identification by ultrasound scanning. In this paper, a fast automatic recognition and location algorithm for fetal genital organs is proposed as an effective method to help prevent ultrasound technicians from unethically and illegally identifying the sex of the fetus. This automatic recognition algorithm can be divided into two stages. In the 'rough' stage, a few pixels in the image, which are likely to represent the genital organs, are automatically chosen as points of interest (POIs) according to certain salient characteristics of fetal genital organs. In the 'fine' stage, a specifically supervised learning framework, which fuses an effective feature data preprocessing mechanism into the multiple classifier architecture, is applied to every POI. The basic classifiers in the framework are selected from three widely used classifiers: radial basis function network, backpropagation network, and support vector machine. The classification results of all the POIs are then synthesized to determine whether the fetal genital organ is present in the image, and to locate the genital organ within the positive image. Experiments were designed and carried out based on an image dataset comprising 658 positive images (images with fetal genital organs) and 500 negative images (images without fetal genital organs). The experimental results showed true positive (TP) and true negative (TN) results from 80.5% (265 from 329) and 83.0% (415 from 500) of samples, respectively. The average computation time was 453 ms per image.

关键词： Ultrasound image Fetal genital organ Point of interest (POI) Feature selection Feature extraction Class imbalance Multiple classifier architecture

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Preparation and Optical Properties of SnO2／siO2 Nanocomposite

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Chinese Physics Letters 2004年第7期21卷 1374-1376页

作者： FENGYi-si YAORi-sheng ZHANGLi-De SchoolofChemicalEngineering HefeiUniversityofTechnologyAnhui230009 InstituteofSolidStatePhysics ChineseAcademyofSciencesPO1129BoxHefei230031

SnO2/siO2 nanocomposites have been prepared by the soaking-thermal-decomposing method, tin oxide nanoparticles are uniformly dispersed in the mesopores of silica. The optical absorption edge of the obtained nanocomposite presents a redshift compared with bulk tin oxide. With the increasing annealing temperature during the procedure of the sample preparation, the optical absorption edge of the sample moves to shorter wavelength (blueshift). These optical properties can be ascribed to the amorphous structure and band defects of surface layers of the tin oxide nanoparticles.

关键词： Nanocomposites

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