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清营生脉汤激活Akt信号通路保护H9c2心肌细胞缺氧复氧损伤的作用研究

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中国中医急症 2024年第5期33卷 804-808页

作者：谢蝶付殷赵东张璐艳张洋黑龙江中医药大学黑龙江哈尔滨150006

目的研究清营生脉汤含药血清对缺氧复氧诱导H9c2心肌细胞损伤的保护机制。方法本研究采用H9c2心肌细胞缺氧/复氧(H/R)方法模拟心肌缺血再灌注损伤(MIRI)细胞模型,细胞实验分组为:空白血清对照组、模型组、含药血清低剂量组、含药血清中... 详细信息

目的研究清营生脉汤含药血清对缺氧复氧诱导H9c2心肌细胞损伤的保护机制。方法本研究采用H9c2心肌细胞缺氧/复氧(H/R)方法模拟心肌缺血再灌注损伤(MIRI)细胞模型,细胞实验分组为:空白血清对照组、模型组、含药血清低剂量组、含药血清中剂量组、含药血清高剂量组。应用CCK-8法测定心肌细胞活性,流式细胞术检测细胞凋亡率,ELISA法检测超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)、丙二醛(MDA)、肌酸激酶同工酶(CK-MB)水平,Western blotting法检测细胞内磷脂酰肌醇3-激酶(PI3K)、蛋白激酶B(Akt)及p-Akt的蛋白表达。结果与对照组相比,模型组细胞存活率显著降低、凋亡率显著升高、细胞内SOD水平显著降低,LDH、MDA、CK-MB水平显著升高(P<0.01);与模型组相比,含药血清低、中、高剂量组细胞的存活率显著升高、凋亡率显著降低、细胞内SOD水平显著升高,LDH、MDA、CK-MB水平显著降低(P<0.01);与对照组相比,模型组细胞中p-Akt蛋白表达水平显著降低(P<0.01),p-Akt/Akt比值降低(P<0.05);与模型组相比,含药血清高剂量组细胞内p-Akt蛋白表达量显著升高(P<0.01),p-Akt/Akt比值明显升高(P<0.05)。结论清营生脉汤含药血清能够提高缺氧复氧心肌细胞的活性,降低细胞凋亡率,升高SOD水平,降低LDH、MDA、CK-MB水平,提高细胞抗氧化能力,且可能通过促进Akt磷酸化降低缺氧复氧诱导的H9c2心肌细胞损伤程度。

关键词：心肌缺血再灌注损伤清营生脉汤含药血清 H9c2 大鼠

便携式呼吸机辅助标准心肺复苏护理对改善小儿危重症体征及预后的影响研究

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黑龙江医学 2024年第10期48卷 1239-1241页

作者：贾梦洁王云华张陆燕黄河三门峡医院门诊部河南三门峡472000 黄河三门峡医院儿科输液河南三门峡472000 黄河三门峡医院儿科重症监护室河南三门峡472000

目的:观察便携式呼吸机辅助标准心肺复苏护理对危重症患儿体征及预后的影响。方法:选取2019年5月—2022年1月黄河三门峡医院收治的103例危重症患儿作为研究对象,采用抽签法将其分为对照组和观察组。对照组(51例)均接受标准心肺复苏护理... 详细信息

目的:观察便携式呼吸机辅助标准心肺复苏护理对危重症患儿体征及预后的影响。方法:选取2019年5月—2022年1月黄河三门峡医院收治的103例危重症患儿作为研究对象,采用抽签法将其分为对照组和观察组。对照组(51例)均接受标准心肺复苏护理,观察组(52例)采用便携式呼吸机辅助标准心肺复苏护理,比较两组患儿的呼吸功能、氧合功能、基础体征、血气指标及预后情况。结果:观察组患儿静态肺潮气容量(VT)、每分钟最大通气量(MVV)、第1秒最大呼气容积(FEV_(1))、用力肺活量(FVC)高于对照组,差异有统计学意义(t=2.671、2.871、2.285、12.300,P<0.05);观察组患儿动脉氧气含量(CaO_(2))、静脉氧气含量(CvO_(2))、氧合指数(P/F)高于对照组,呼吸指数(RI)低于对照组,差异有统计学意义(t=3.107、2.098、3.018、2.801,P<0.05);观察组的心率(HR)、平均动脉压(MAP)、血氧饱和度(SaO_(2))高于对照组,呼吸频率(PR)低于对照组,差异有统计学意义(t=3.265、2.738、2.931、2.989,P<0.05);观察组患儿动脉血氧分压(PO_(2))高于对照组,动脉二氧化碳分压(PCO_(2))、酸碱值(pH)、碳酸氢根(HCO_(3))低于对照组,差异有统计学意义(t=2.440、3.099、2.689、2.269,P<0.05);观察组患儿不良事件发生率低于对照组,差异有统计学意义(χ^(2)=5.137,P<0.05)。结论:采用便携式呼吸机辅助标准心肺复苏可有效改善心脏骤停危重症患儿的呼吸功能及氧合功能,对促进患儿基础体征、血气指标恢复并降低不良事件发生风险均有积极意义。

关键词：小儿危重症心脏骤停标准心肺复苏便携式呼吸机护理效果

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HEPS六极铁标定方案分析

核技术 2024年第2期47卷 41-48页

作者：韩圆颖吴亚峰董岚王铜卢尚闫路平张露彦刘晓阳闫皓月马娜何振强柯志勇门玲鸰李波王小龙梁静中国科学院高能物理研究所北京100049 散裂中子源科学中心东莞523803

六极铁作为高能同步辐射光源(High Energy Photon Source,HEPS)储存环八铁单元的重要部件之一,其技术工艺较为复杂,对加工精度和中心引出精度要求都很高。本文对HEPS六极铁的机械中心引出标定方案进行了研究,利用极缝偏差角对常规标定... 详细信息

六极铁作为高能同步辐射光源(High Energy Photon Source,HEPS)储存环八铁单元的重要部件之一,其技术工艺较为复杂,对加工精度和中心引出精度要求都很高。本文对HEPS六极铁的机械中心引出标定方案进行了研究,利用极缝偏差角对常规标定坐标系进行旋转,使三个极缝面更接近理论位置,从而减小磁铁主场斜分量;对每块磁铁进行两次机械中心标定,极缝间距的实测值与设计值的标准偏差在0.015 mm,坐标系旋转前后的基准点偏差标准值为0.09 mm,旋转角最大可达0.6 mrad。这种考虑极缝偏差角的建系方法可以提高标定的精度,能为实际工作中同类型、准直精度有相同要求的设备标定提供参考,有利于加速器装置的顺利安装,对加速器准直测量具有十分重要的意义。

关键词：六极铁中心标定机械中心坐标系旋转准直精度

The Statistical Power of Inclusive Composite Interval Mapping in Detecting Digenic Epistasis Showing Common F_2 Segregation Ratios

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Journal of Integrative Plant Biology 2012年第4期54卷 270-279页

作者： luyan zhang Huihui Li Jiankang Wang Institute of Crop Sciences The National Key Facility for Crop Gene Resources and Genetic Improvementand CIMMYT ChinaChinese Academy of Agricultural SciencesBeijing 100081China

Epistasis is a commonly observed genetic phenomenon and an important source of variation of complex traits, which could maintain additive variance and therefore assure the long-term genetic gain in breeding. Inclusive composite interval mapping （ICIM） is able to identify epistatic quantitative trait loci （QTLs） no matter whether the two interacting QTLs have any additive effects. In this article, we conducted a simulation study to evaluate detection power and false discovery rate （FDR） of ICIM epistatic mapping, by considering F2 and doubled haploid （DH） populations, different F2 segregation ratios and population sizes. Results indicated that estimations of QTL locations and effects were unbiased, and the detection power of epistatic mapping was largely affected by population size, heritability of epistasis, and the amount and distribution of genetic effects. When the same likelihood of odd （LOD） threshold was used, detection power of QTL was higher in F2 population than power in DH population; meanwhile FDR in F2 was also higher than that in DH. The increase of marker density from 10 cM to 5 cM led to similar detection power but higher FDR. In simulated populations, ICIM achieved better mapping results than multiple interval mapping （MIM） in estimation of QTL positions and effect. At the end, we gave epistatic mapping results of ICIM in one actual population in rice （Oryza sativa L.）.

关键词： Epistasis false discovery rate inclusive composite interval mapping power analysis simulation study.

Linkage analysis and integrated software GAPL for pure-line populations derived from four-way and eight-way crosses

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The Crop Journal 2019年第3期7卷 283-293页

作者： luyan zhang Lei Meng Jiankang Wang The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences

Pure lines derived from multiple parents provide abundant variation for genetic ***,efficient genetic analysis methods and user-friendly software are still *** this study, we developed linkage analysis methods and integrated analysis software for pure-line populations derived from four-way and eight-way ***, polymorphic markers are classified into different categories according to the number of identifiable alleles in the inbred *** genotypic probability is then derived for each pair of complete markers, and based on them a maximum likelihood estimate(MLE) of recombination frequency is *** EM algorithm is proposed for calculating recombination frequencies in scenarios that at least one marker is incomplete.A linkage map can thus be constructed using estimated recombination *** describe a software package called GAPL for recombination frequency estimation and linkage map construction in multi-parental pure-line *** simulation studies and results from a reported four-way cross recombinant inbred line population demonstrate that the proposed method and software can build more accurate linkage maps in shorter times than other published software *** GAPL software is freely available from *** and can also be used for QTL mapping in multi-parental populations.

关键词： Pure lines Four-way cross Eight-way cross Recombination frequency estimation Integrated software

Ordering of high-density markers by the k-Optimal algorithm for the traveling-salesman problem

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The Crop Journal 2020年第5期8卷 701-712页

作者： luyan zhang Huihui Li Lei Meng Jiankang Wang National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Construction of accurate and high-density linkage maps is a key research area of *** investigated the efficiency of genetic map construction(MAP)using modifications of the k-Optimal(k-Opt)algorithm for solving the traveling-salesman problem(TSP).For TSP,different initial routes resulted in different optimal *** most optimal solution could be found only by use of as many initial routes as *** for MAP,a large number of initial routes resulted in one optimal order.k-Opt using open route length gave a slightly higher proportion of correct orders than the method of adding one virtual marker and using closed route *** frequency(REC)and logarithm of odds(LOD)score gave similar proportions of correct order,higher than that given by genetic *** missing markers and genotyping error reduced ordering accuracy,but the best order was still achieved with high probability by comparison of the optimal orders from multiple initial *** time increased rapidly with marker number,and 2-Opt took much less time than *** 2-Opt algorithm was compared with ordering methods used in two other software *** best method was 2-Opt using open route length as the criterion to identify the optimal order and using REC or LOD as the measure of distance between *** describe a unified software interface for using k-Opt in high-density linkage map construction for a wide range of genetic populations.

关键词： optimal algorithm traveling

Estimation of statistical power and false discovery rate of QTL mapping methods through computer simulation

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Chinese Science Bulletin 2012年第21期57卷 2701-2710页

作者： LI HuiHui zhang luyan WANG JianKang Institute of Crop Sciences The National Key Facility for Crop Gene Resources and Genetic Improvementand C1MMYT ChinaChinese Academyof Agricultural SciencesBeijing 100081China

Many QTL mapping methods have been developed in the past two ***,the best method should have a high detection power but a low false discovery rate (FDR).Power and FDR cannot be derived theoretically for most QTL mapping methods,but they can be properly evaluated using computer *** this paper,we used four genetic models (two for independent loci and two for linked loci) to illustrate power and FDR estimation for interval mapping (IM) and inclusive composite interval mapping (ICIM).For each model,we simulated 1000 populations each of 200 doubled haploids.A support interval (SI) was first defined to indicate to which predefined QTL the significant QTL *** was calculated by counting the number of simulation runs with significant peaks higher than the logarithm of odds (LOD) threshold in the *** trait loci not identified in any SIs were viewed as false *** FDR is the rate at which QTLs are identified as significant when they are actually *** results allowed us to estimate power and FDR of IM and ICIM for two independent and two linkage genetic *** estimates allowed us to readily compare the efficiencies of different statistical methods for QTL mapping,including the ability to separate linkage,under a wide range of genetic *** used IM and ICIM as examples of how to estimate power and FDR,but the principles shown in this paper can be used for power analysis and comparison of any other QTL mapping methods,especially those based on interval tests.

关键词： QTL定位计算机模拟定位方法统计方法估计假发遗传模型复合区间作图

QTL IciMapping:Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations

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The Crop Journal 2015年第3期3卷 269-283页

作者： Lei Meng Huihui Li luyan zhang Jiankang Wang The National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop Science and CIMMYT China OfficeChinese Academy of Agricultural Sciences

QTL Ici Mapping is freely available public software capable of building high-density linkage maps and mapping quantitative trait loci(QTL) in biparental populations. Eight functionalities are integrated in this software package:(1) BIN: binning of redundant markers;(2) MAP: construction of linkage maps in biparental populations;(3) CMP: consensus map construction from multiple linkage maps sharing common markers;(4) SDL: mapping of segregation distortion loci;(5) BIP: mapping of additive, dominant, and digenic epistasis genes;(6) MET: QTL-by-environment interaction analysis;(7) CSL: mapping of additive and digenic epistasis genes with chromosome segment substitution lines; and(8) NAM: QTL mapping in NAM populations. Input files can be arranged in plain text, MS Excel 2003, or MS Excel 2007 formats. Output files have the same prefix name as the input but with different extensions. As examples, there are two output files in BIN, one for summarizing the identified bin groups and deleted markers in each bin, and the other for using the MAP functionality. Eight output files are generated by MAP, including summary of the completed linkage maps, Mendelian ratio test of individual markers, estimates of recombination frequencies, LOD scores, and genetic distances, and the input files for using the BIP, SDL,and MET functionalities. More than 30 output files are generated by BIP, including results at all scanning positions, identified QTL, permutation tests, and detection powers for up to six mapping methods. Three supplementary tools have also been developed to display completed genetic linkage maps, to estimate recombination frequency between two loci,and to perform analysis of variance for multi-environmental trials.

关键词： Biparental populations Map construction QTL mapping Software

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高能光源磁铁的三坐标标定技术

强激光与粒子束 2024年第9期36卷 60-65页

作者：韩圆颖董岚王铜卢尚闫路平张露彦刘晓阳闫皓月门玲鸰王小龙李波梁静马娜何振强柯志勇中国科学院高能物理研究所北京100049 东莞中子科学中心广东东莞523803

针对高能同步辐射光源需要进行大批量磁铁的中心引出标定,提出了一种基于三坐标测量机的磁铁机械中心标定方案,确定了编写自动测量程序的流程和方法 ,参与完成了加速器中多种类型磁铁的标定。对176块储存环六极铁的三坐标机测量数据进... 详细信息

针对高能同步辐射光源需要进行大批量磁铁的中心引出标定,提出了一种基于三坐标测量机的磁铁机械中心标定方案,确定了编写自动测量程序的流程和方法 ,参与完成了加速器中多种类型磁铁的标定。对176块储存环六极铁的三坐标机测量数据进行分析,结果表明:每块磁铁进行两遍机械中心标定,获得准直基准点的标定重复性在0.01 mm之内;极缝间距离的实测值与设计值标准偏差均在0.015 mm之内;标定效率相较激光跟踪仪提高了2倍。这种借助三坐标测量机的磁铁标定方法可以提高标定精度,降低人力成本,提高工作效率,能为加速器工程中磁铁中心的引出标定工作提供参考,保证加速器装置的安装顺利,满足加速器准直测量的工程进度要求。

关键词：三坐标测量机机械中心标定加速器准直测量