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成年大鼠心室肌细胞的培养

汕头大学医学院学报 2014年第3期27卷 148-149,F0002页

作者：郑燕珊刘冰 lutz pott 郑付春高分飞汕头大学医学院药理学教研室 Institute of Physiology Ruhr-University BochumD-44780 BochumGermany 汕头大学医学院第一附属医院药剂科

目的:探讨成年大鼠心室肌细胞的分离培养方法,提高细胞存活时间。方法:采用LANGENDORFF灌流法,胶原酶P酶解分离心室肌细胞,并用M199培养基进行培养。显微镜下观察细胞形态结构,并予电刺激观察心室肌细胞的收缩情况。结果:心室肌细胞分... 详细信息

目的:探讨成年大鼠心室肌细胞的分离培养方法,提高细胞存活时间。方法:采用LANGENDORFF灌流法,胶原酶P酶解分离心室肌细胞,并用M199培养基进行培养。显微镜下观察细胞形态结构,并予电刺激观察心室肌细胞的收缩情况。结果:心室肌细胞分离、存活率高,形态结构良好,存活可达7 d。电刺激下心室肌细胞出现同步收缩;若去除细胞外液中的钙离子,则不再收缩。表明心室肌细胞活性完好。结论:改进后的分离和培养方法,能使急性分离的成年大鼠心肌细胞的存活时间明显延长。

关键词：心肌细胞成年酶解分离培养

Abiotic drivers shape seed inputs and outputs in a tropical wetland on Croton trinitatis population

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Ecological Processes 2022年第1期11卷 65-77页

作者： Francielli Bao Arnildo pott Pedro Manuel Villa Centro de Avaliação da Biodiversidade Pesquisa e Conservação do Cerrado(CBC)Instituto Chico Mendes de Conservação da Biodiversidade(ICMBio)BrasíliaDF 70670-350Brazil Programa de Pós-Graduação em Biologia Vegetal Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulCampo GrandeMS 79070-900Brazil Programa de Pós-Graduacão em Botânica Departamento de Biologia VegetalUniversidade Federal de ViçosaViçosaMG 36570-900Brazil Fundación para la Conservación de la Biodiversidad Mérida 7101Venezuela

Background:Predicting how natural and anthropogenic drivers shape different ecological indicators,such as plant populations along environmental gradients,can be a relevant tool for establishing management and conservation criteria of tropical *** aimed to assess the effects of seasonal flood disturbance,type of grasslands and topographical conditions on Croton trinitatis population distribution in a tropical ***:The study was carried out in a seasonally flooded grassland(Central-West Brazil).We conducted samplings of soil on the dry and flood hydrophases of the *** took the samples in eight seasonal ponds,with 1 km interval between *** were marked during the flood period,observing the water level,one in the lowest zone,in the middle of the pond=low(ca.60 cm deep),one at the pond edge=mid(ca.30 cm deep)and one in the higher zone,on the external part=high(ca.1 cm deep).Results:The results showed that the topography,seasonality,and types of grassland determine differences in the abundance patterns of adult plants and seedlings,and seed bank and seed *** abiotic factors can shape plant population-related ecological processes and patterns,with outputs(germination and predation)and inputs(local dispersion and from neighbouring areas)of proportional seeds for the population maintenance in this ***:We emphasize the importance of these findings,to show that abiotic factors are not the only ones to be considered in ecological studies of distribution and structuring of populations in habitats with extreme seasonal events.

关键词： Environmental filter Flood disturbance Pantanal Population ecology Seasonal change Seed predation Seedling Soil seed bank

Differences between species in seed bank and vegetation helps to hold functional diversity in a floodable Neotropical savanna

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Journal of Plant Ecology 2021年第4期14卷 605-615页

作者： Evaldo Bde Souza Francielli Bao Geraldo A.Damasceno Junior Arnildo pott Programa de Pos-graduacao em Ecologia e Conservacao Instituto de BiocienciasUniversidade Federal de Mato Grosso do SulCampo GrandeBrazil Programa de Pos-graduacao em Biologia Vegetal Instituto de BiocienciasUniversidade Estadual PaulistaRio ClaroSPBrazil Programa de Pos-graduacao em Biologia Vegetal Instituto de BiociênciasUniversidade Federal de Mato Grosso do SulCampo GrandeBrazil

Aims Our objective was to quantify the contributions of the seed bank and the established vegetation to the species composition,functional composition and diversity,and discuss the implications of these differences in regeneration and persistence of floodplain plant *** We sampled all ground cover vegetation up to 1.5 m height and seed bank in 25 plots(10 m×1 m)distributed across five sites in dry and rainy seasons in a periodically flooded savanna in the Pantanal wetland,*** evaluated the soil seed bank by seedling emergence *** Findings The seed bank species had traits that conferred regeneration to the communities,while persistence traits characterized the *** seed bank had higher functional richness and lower functional evenness than the *** existence of different plant traits between seed bank and vegetation allowed the coexistence of species with functionally contrasting persistence and regeneration traits,which may help maintain functional *** may allow the community to be more resilient when dealing with different environmental filters such as drought,fire and flood.

关键词： environmental filters functional diversity hydrochoric dispersal Pantanal wetland persistence regeneration

人类活动影响下的生物多样性保护:中欧的植被演化及其启示

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生态学杂志 2011年第3期30卷 584-588页

作者： Richard pott 刘鸿雁印轶 Institute for Geobotany Leibniz University of Hannover 北京大学城市与环境学院北京100871

对历史时期欧洲中部地区植被历史的研究表明,人类活动不仅破坏了自然、导致植被的单一化,同时也对植被与景观的丰富度(多样性)产生影响。人类或家畜的干预使得一些半自然、高多样性的生境类型及相应的植被类型得以保持和发展;但过度的... 详细信息

对历史时期欧洲中部地区植被历史的研究表明,人类活动不仅破坏了自然、导致植被的单一化,同时也对植被与景观的丰富度(多样性)产生影响。人类或家畜的干预使得一些半自然、高多样性的生境类型及相应的植被类型得以保持和发展;但过度的干扰势必造成植被的单一化和贫乏化,威胁生物多样性的保存和保护。当今的中欧植被格局总体上是近几个世纪,某些地区甚至是上千年来,人为干扰叠加在气候变化影响之上的综合产物。中欧大部分地区的潜在植被多为森林,如果没有人为活动的强烈影响,除了岩石、水体、海滩、林线以上的高山以及高位沼泽等特定生境的分布区域以外,当今中欧的大部分地区应当仍覆盖着郁闭的阔叶林,而不会表现出今天的多样性景观。因此,自然保护的对象不应当仅仅是"纯自然"植被,保护手段也不仅仅是"清除人为干扰"。在保护多样性思想的指导下,各种典型植被类型都是值得保护的,除了高自然度的自然生态系统外,栽培植被也应当被保存和保护,否则,它们会随着特定耕作或管理方式的消失而消失。同时,既应保护"当前植被",也要恢复"潜在植被",使目前多样的景观类型、植被类型和栖息地类型得以维持和发展。本文结论为解决当前欧洲自然保护中的争议性问题提供了依据,也可以为中国的生物多样性保护战略提供参考。

关键词：生物多样性保护植被历史自然度人类干扰欧洲

Management, Triage and Outcomes of 378 Patients Presenting to the Emergency Department with Chest Pain of Acute Onset： A Single Centre Observational Study

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Journal of Health Science 2014年第11期2卷 523-528页

作者： Samira Green Vanessa Jessop Jason pott Tim Harris University Hospitals Leicester Leicester LE1 5WW UK Royal London Hospital London E1 1BB UK

Chest pain accounts for 700,000 hospital attendances in the UK each year. Prompt risk assessment and diagnosis both improves outcomes and reduces unnecessary admissions. Data on patients who presented to the ED with chest pain was collected retrospectively. Patients were then followed up via hospital records and telephone. 70% of the authors＇ patients were triaged as low risk chest pain. Three represented within 30 d with non ischaemic chest pain. 1 patient had MACE. The authors＇ triage pathway safely managed those presenting with chest pain.

关键词： Acute coronary syndrome triage near patient testing troponin I risk stratification.

Single-centre all-comers registry reveals promising long-term clinical results of the Endeavor<sup>TM</sup>-Stent

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World Journal of Cardiovascular Diseases 2012年第2期2卷 82-89页

作者： Klaus Hertting Agneta Wieneke Daniel Hausmann Claudia Zeiler Christian pott Werner Raut Department of Cardiology Krankenhaus Buchholz Buchholz Germany

Background: To evaluate the safety and efficacy of the EndeavorTM-Stent-Stent (Medtronic Corp., USA) in daily practice. Material and methods: Data come from a prospective single-centre registry. Between 2005 and 2007 all patients, who received at least one Endeavor? coronary stent, were included into a registry. Patients were contacted after 12 and 24 - 36 months. Results: 326 patients (97 females, mean age 67.5 ± 10.3 years) were included. From these patients 137 (42%) had a 3-vessel disease, 96 (29%) presented with myocardial infarction ≤ 72 hrs, 19 (6%) with a left ventricular ejection fraction TM-Stent-Stents. Per patient, 0.4 ± 0.7 bare metal stents were implanted. Median follow-up time was 35.3 months, during follow-up 165 patients (50.6%) had repeated angiography. After 12 (24) months overall mortality was 5.8% (7.3%), cardiac mortality 3.1% (3.5%), myocardial infarction rate 1.5% (4.1%), target-vessel revascu-larization rate 8.7% (12.4%), target-lesion revascu-larization rate 6.1% (8.4%), and cumulative MACE-rate 14.1% (21.9%). In total, 2 definite or likely stent-thromboses (0.6%) occurred during follow-up. Logistic regression revealed the treatment of saphenous vein grafts and aorto-coronary ostial lesions as risk factors for target lesion revascularization. Conclusion: The EndeavorTM-Stent-Stent is a safe and effective drug-eluting stent in the treatment of daily-life coronary patients. Treatment of vein grafts and aorto-coronary ostial lesions appear to be associated with a higher need for re-interventions.

关键词： Zotarolimus Restenosis Drug-Eluting Stent

路面水深,速度,胎面厚度,内压和负荷对载重轮胎湿地牵引性能的影响

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轮胎研究与开发 1999年第4期25卷 36-42页

作者：吴荻 pott,GM

The GlideScope<sup>®</sup>Video Laryngoscope and the Macintosh Laryngoscope Compared in a Simulated Difficult Airway with Immobilization and Bleeding—A Randomized, Prospective, Crossover Study

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Open Journal of Anesthesiology 2012年第2期2卷 23-28页

作者： Wiebke Ackermann Leonardus M. pott Sonia J. Vaida Arne O. Budde Milton S. Hershey Medical Center—Department of Anesthesiology Penn State University Columbus USA. Milton S. Hershey Medical Center—Department of Anesthesiology Penn State University Hershey USA

Background: This study assesses the effectiveness of the GlideScope? Videolaryngoscope (GS) in comparison with the Macintosh laryngoscope in a simulated difficult airway with blood in the airway and restricted range of motion of the neck. 39 participants experienced with the GS and the Macintosh laryngoscope were used. Methods: This analysis is a prospective, randomized, crossover study. Our study was performed on an intubation simulation model with artificial blood in the airway and restricted range of motion. The intubation time was recorded from picking up the laryngoscope to advancing the endotracheal tube through the glottic opening. Secondary endpoints were the Cormack & Lehane score, the percentage of the glottis seen, the subjective difficulty of the procedure on a scale of 0 to 10, the number of adjustment maneuvers, the number of attempts, and the number of failed intubations. Attempts were defined as removal of instruments from the airway and reinsertion. Failed intubations were defined as esophageal intubations or intubations lasting longer than 120 seconds. Results: The mean intubation time was 47.6 seconds with the GS and 21.4 seconds with the Macintosh laryngoscope. There were 3 failed intubations with the Macintosh laryngoscope and 4 failed intubations with the GS. The failed intubations with the Macintosh laryngoscope were all esophageal intubations. The failed intubations with the GS were due to exceeding the time limit of 120 seconds. Both devices had a mean Cormack & Lehane score of 1.8 and the mean percentage of the Glottis seen was 58% for both devices. The average subjective difficulty on a scale from 0 to 10 was ranked 4.16 for the Macintosh and 5.14 for the GS. Participants needed an average of 1 adjustment maneuvers with the Macintosh laryngoscope and 2.7 adjustments with the GS. Conclusion: The GS, used by experienced anesthesiologists in a simulated difficult airway, had an inferior performance compared to the Macintosh laryngoscope in te

关键词： GlideScope Difficult Airway

Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit

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Horticulture Research 2019年第1期6卷 1638-1654页

作者： JoséG.Vallarino Delphine M.pott Eduardo Cruz-Rus Luis Miranda Juan J.Medina-Minguez Victoriano Valpuesta Alisdair R.Fernie JoséF.Sánchez-Sevilla Sonia Osorio Iraida Amaya Department of Molecular Biology and Biochemistry Instituto de Hortofruticultura Subtropical y Mediterránea“La Mayora”University of Málaga–Consejo Superior de Investigaciones Científicas(IHSM-UMA-CSIC)Campus de Teatinos29071 MálagaSpain Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany Genómica y Biotecnología Centro de MálagaInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)29140 MálagaSpain Ingeniería y Tecnología Agroalimentaria Centro Las Torres-TomejilInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)Alcaládel RíoSevillaSpain Ingeniería y Tecnología Agroalimentaria Centro de HuelvaInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)HuelvaSpain

Improvement of nutritional and organoleptic quality of fruits is a key goal in current strawberry breeding *** ratio of sugars to acids is a determinant factor contributing to fruit liking,although different sugars and acids contribute in varying degrees to this complex trait.A segregating F1 population of 95 individuals,previously characterized for several fruit quality characters,was used to map during 2 years quantitative trait loci(QTL)for 50 primary metabolites,L-ascorbic acid(L-AA)and other related traits such as soluble solid content(SSC),titratable acidity(TA),and pH.A total of 133 mQTL were detected above the established thresholds for 44 *** 12.9%of QTL were detected in the 2 years,suggesting a large environmental influence on primary metabolite *** objective of this study was the identification of key metabolites that were associated to the overall variation in SSC and *** it was observed in previous studies,a number of QTL controlling several metabolites and traits were co-located in homoeology group V(HG V).mQTL controlling a large variance in raffinose,sucrose,succinic acid,and L-AA were detected in approximate the same chromosomal regions of different homoeologous linkage groups belonging to HG *** genes for selected mQTL are proposed based on their co-localization,on the predicted function,and their differential gene expression among contrasting F1 progeny ***-seq analysis from progeny lines contrasting in L-AA content detected 826 differentially expressed genes and identified Mannose-6-phosphate isomerase,FaM6PI1,as a candidate gene contributing to natural variation in ascorbic acid in strawberry fruit.

关键词： straw breeding trait