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Effects of 1-octyl-3-methylimidazolium nitrate on the microbes in brown soil

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Journal of Environmental Sciences 2018年第5期30卷 249-259页

作者： Cheng Zhang Jun Wang lusheng zhu Zhongkun Du Jinhua Wang Xi Sun Tongtong Zhou Key Laboratory of Agriculture Environment in Universities of Shandong College of Resources and Environment Shandong Agricultural University

The toxicity of ionic liquids（ILs） on soil organisms has aroused wide attention due to their high-solubility. The present investigation focused on the toxicity of 1-octyl-3-methylimidazolium nitrate（[C8mim]NO3） on the microbial populations（bacteria, fungi, and actinomycetes）, soil enzyme（urease, dehydrogenase, acid phosphatase, and β-glucosidase）activities, microbial community diversity using terminal restriction fragment length polymorphism（T-RFLP）, and abundance of the ammonia monooxygenase（amoA） genes of ammonia-oxidizing archaea（AOA） and ammonia-oxidizing bacteria（AOB） using quantitative real-time polymerase chain reaction（q-PCR） in brown soil at each trial with doses of 0, 1.0, 5.0,and 10.0 mg/kg on days 10, 20, 30, and 40. The contents of [C8mim]NO3 in soil were measured using high performance liquid chromatography with recoveries of 84.3% to 85.2%, and changed less than 10% during the experimental period. A significant decrease was observed from the bacteria, fungi and actinomycetes populations at 10.0 mg/kg, at which the urease activity was inhibited and the β-glucosidase activity was stimulated on days 20, 30, and 40. In addition, [C8mim]NO3 inhibited the dehydrogenase activity at 10 mg/kg on days 30 and 40 and the acid phosphatase activity on day 20. The diversity of the soil microbial community and the gene abundance of AOA-and AOB-amo A were also inhibited. Furthermore, the present investigation provided more scientific information for the toxicity evaluation of ILs in soil.

关键词： Ionic liquids Quantitative real-time polymerasechain reaction Functional gene High performance liquidchromatography (HPLC) β-Glucosidase

Biodegradation of organochlorine pesticide endosulfan by bacterial strain Alcaligenes faecalis JBW4

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Journal of Environmental Sciences 2013年第11期25卷 2257-2264页

作者： Lingfen Kong Shaoyuan zhu lusheng zhu Hui Xie Kunchang Su Tongxiang Yan Jun Wang Jinhua Wang Fenghua Wang Fengxia Sun Key Laboratory of Agricultural Environment in Universities of Shandong National Engineering Laboratory for Efcient Utilization of Soil and Fertilizer Resources College of Resources and Environment Shandong Agriculture University College of Marine Life Sciences Ocean University of China

The recently discovered endosulfan-degrading bacterial strain Alcaligenesfaecalis JBW4 was isolated from activated sludge. This strain is able to use endosulfan as a carbon and energy source. The optimal conditions for the growth of strain JBW4 and for biodegradation by this strain were identified, and the metabolic products of endosulfan degradation were studied in detail. The maximum level of endosulfan biodegradation by strain JBW4 was obtained using broth at an initial pH of 7.0, an incubation temperature of 40℃ and an endosulfan concentration of I00 mg/L. The concentration of endosulfan was determined by gas chromatography. Strain JBW4 was able to degrade 87.5% of α-endosulfan and 83.9% of β-endosulfan within 5 days. These degradation rates are much higher than the previously reported bacterial strains. Endosulfan diol and endosulfan lactone were the major metabolites detected by gas chromatography-mass spectrometry; endosulfan sulfate, which is a persistent and toxic metabolite, was not detected. These results suggested that A. faecalis JBW4 degrades endosulfan via a non-oxidative pathway. The biodegradation of endosulfan by A. faecalis is reported for the first time. Additionally, the present study indicates that strain JBW4 may have potential for the biodegradation of endosulfan residues.

关键词： bacterial biodegradation endosulfan Alcaligenesfaecalis optimal conditions endosulfan diol non-oxidative pathway

Effect of sulfamethazine on the horizontal transfer of plasmid-mediated antibiotic resistance genes and its mechanism of action

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Journal of Environmental Sciences 2023年第5期127卷 399-409页

作者： Xiaojing Yan Wenwen Liu Shengfang Wen Lanjun Wang lusheng zhu Jun Wang Young Mo Kim Jinhua Wang National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources Key Laboratory of Agricultural Environment in Universities of ShandongCollege of Resources and EnvironmentShandong Agricultural UniversityTai’an 271018China Department of Civil and Environmental Engineering Hanyang UniversitySeongdong-guSeoul 04763Korea

As a new type of environmental pollutant,antibiotic resistance genes(ARGs)pose a huge challenge to global *** gene transfer(HGT)represents an important route for the spread of *** widespread use of sulfamethazine(SM2)as a broad-spectrum bacteriostatic agent leads to high residual levels in the environment,thereby increasing the spread of ***,we chose to study the effect of SM2 on the HGT of ARGs mediated by plasmid RP4 from Escherichia coli(***)HB101 to *** NK5449 as well as its mechanism of *** results showed that compared with the control group,SM2 at concentrations of 10 mg/L and 200 mg/L promoted the HGT of ARGs,but transfer frequency decreased at concentrations of 100 mg/L and 500 mg/*** transfer frequency at 200 mg/L was 3.04×10^(−5),which was 1.34-fold of the control *** mechanism of SM2 improving conjugation transfer is via enhancement of the mRNA expression of conjugation genes(trbBP,trfAP)and oxidative stress genes,inhibition of the mRNA expression of vertical transfer genes,up regulation of the outer membrane protein genes(ompC,ompA),promotion of the formation of cell pores,and improvement of the permeability of cell membrane to promote the conjugation transfer of plasmid *** results of this study provide theoretical support for studying the spread of ARGs in the environment.

关键词： Sulfamethazine Plasmid Antibiotic resistance gene Conjugation transfer

Enhanced remediation of chlorpyrifos-contaminated soil by immobilized strain Bacillus H27

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Journal of Environmental Sciences 2024年第10期144卷 172-184页

作者： Changrui Liu Shengfang Wen Shuhan Li Yu Tian Lanjun Wang lusheng zhu Jun Wang Young Mo Kim Jinhua Wang National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources Key Laboratory of Agricultural Environment in Universities of ShandongCollege of Resources and EnvironmentShandong Agricultural UniversityTaian 271018China Department of Civil and Environmental Engineering Hanyang UniversitySeongdong-guSeoul 04763Korea

Chlorpyrifos is a pesticide widely used in agricultural production with a relatively long residual half-life in *** the problem of residual chlorpyrifos is of universal *** this study,rice hull biochar was used as an immobilized carrier to prepare the immobilized strain H27 for the remediation of chlorpyrifos-contamination *** microorganisms after remediation were investigated by ecotoxicological *** immobilized strain H27 had the highest removal rate of chlorpyrifos when 10%bacterial solution was added to the liquid medium containing 0.075-0.109 mm diameter biochar cultured for 22 *** study on the removal of chlorpyrifos by immobilized strain H27 showed that the initial concentration of chlorpyrifos in solution was 25mg/L,and the removal rate reached 97.4%after 7 days of *** the soil,the removal rate of the immobilized bacteria group increased throughout the experiment,which was significantly higher than that of the free bacteria and biochar treatment *** Biolog-ECO test,T-RFLP and RT-RCR were used to study the effects of the soil microbial community and nitrogen cycling functional genes during chlorpyrifos *** was found that ICP group had the highest diversity index among the four treatment *** microflora of segment containing 114 bp was the dominant bacterial community,and the dominant microflora of the immobilized bacteria group was more evenly *** influence of each treatment group on ammonia-oxidizing bacteria(AOB)was greater than on ammonia-oxidizing archaea(AOA).This study offers a sound scientific basis for the practical application of immobilized bacteria to reduce residual soil pesticides.

关键词： Chlorpyrifos Immobilized microorganisms Soil remediation Soil microorganism

Immobilization of an enzyme from a Fusarium fungus WZ-I for chlorpyrifos degradation

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Journal of Environmental Sciences 2010年第12期22卷 1930-1935页

作者： Hui Xie,lusheng zhu,Tingting Ma,Jun Wang,Jinhua Wang,Jun Su,Bo Shao College of Resources and Environment,Shandong Agricultural University,Taian 271018,China [a] College of Resources Environment Shandong Agricultural University Taian 271018 China

The free enzyme extracted from WZ-I, which was identified as Fusarium LK. ex Fx, could effectively degrade chlorpyrifos, an organophosphate insecticide. The methods of immobilizing this free enzyme and determined its degradation-related characteristics were investigated. The properties of the immobilized enzyme were compared with those of the free enzyme. The optimal immobilization of the enzyme was achieved in a solution of 30 g/L sodium alginate at 4℃ for 4-12 hr. The immobilized enzyme showed the maximal activity at pH 8.0, 45℃. The maximum initial rate and the substrate concentration of the immobilized enzyme were less than that of the free enzyme. The immobilized enzyme, therefore, had a higher capacity to withstand a broader range of temperatures and pH conditions than the free enzyme. With varying pH and temperatures, the immobilized enzyme was more active than the free enzyme in the degradation reaction. In addition, the immobilized enzyme exhibited only a slight loss in its initial activity, even after three repeated uses. The results showed that the immobilized enzyme was more resistant to different environmental conditions, suggesting that it was viable for future practical use.

关键词： organophosphate pesticide immobilized enzyme degradation fungus

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二甲戊乐灵降解细菌的分离及降解特性

环境科学 2005年第1期26卷 145-149页

作者：朱鲁生林爱军王军程翠花山东农业大学资源与环境学院山东泰安271018 山东华阳集团环保科山东宁阳271400

分离鉴定了二甲戊乐灵降解细菌 ,并研究了培养条件对二甲戊乐灵降解和细菌生长的影响 .结果表明 ,所分离的 2 9株细菌中有 3株在 3d内对 10 0mg·L-1二甲戊乐灵的降解率在 80 %以上 ,选择HB 1、WB 12株细菌进一步研究 ,此 2株细菌经鉴... 详细信息

分离鉴定了二甲戊乐灵降解细菌 ,并研究了培养条件对二甲戊乐灵降解和细菌生长的影响 .结果表明 ,所分离的 2 9株细菌中有 3株在 3d内对 10 0mg·L-1二甲戊乐灵的降解率在 80 %以上 ,选择HB 1、WB 12株细菌进一步研究 ,此 2株细菌经鉴定分别属于假单胞菌 (Pseudomonas)和玫瑰色微球菌 (Mirococcusluteus) .结果表明 2株细菌在中性培养液中外加碳源浓度为0 2 %、二甲戊乐灵浓度不大于 10 0mg·L-1、30～ 4 0℃培养的条件下 ,细菌生长量和二甲戊乐灵的降解率都达到最大 .

关键词：二甲戊乐灵细菌降解生长量

抗生素和铜联合作用对蚕豆根尖细胞微核率的影响

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环境科学研究 2015年第7期28卷 1085-1090页

作者：赵祥王金花朱鲁生王军山东农业大学资源与环境学院山东省高校农业环境重点实验室山东泰安271000 南开大学环境污染过程与基准教育部重点实验室天津300071

为确定抗生素与重金属单一及联合作用对细胞遗传物质的损伤程度,以青皮蚕豆根尖细胞为试验材料,研究四环素类抗生素OTC（土霉素）、CTC（金霉素）、TTC（四环素）和重金属Cu单一以及联合作用对蚕豆根尖细胞微核率和微核指数的影响.结果... 详细信息

为确定抗生素与重金属单一及联合作用对细胞遗传物质的损伤程度,以青皮蚕豆根尖细胞为试验材料,研究四环素类抗生素OTC（土霉素）、CTC（金霉素）、TTC（四环素）和重金属Cu单一以及联合作用对蚕豆根尖细胞微核率和微核指数的影响.结果表明：1抗生素与Cu单独作用,二者c在0~1.6 mmol/L之间时,蚕豆根尖细胞微核率均高于3.33‰,微核指数均大于1.5,与CK（对照处理）相比差异显著（P〈0.01）;随着c（抗生素）的增大,蚕豆根尖细胞微核率呈先减后增的趋势.2抗生素与Cu联合作用时,蚕豆根尖细胞微核率和微核指数均小于单独作用时二者之和,交互作用表现为拮抗作用,其中c（抗生素）∶c（Cu）为1∶4时拮抗作用最明显,c（抗生素）∶c（Cu）为1∶1和1∶2时根尖细胞微核率呈减少的趋势,c（抗生素）∶c（Cu）为1∶8和1∶16时根尖细胞微核率呈增加的趋势.研究显示,四环素类抗生素与Cu单一及联合作用于蚕豆根尖细胞时,均会对植物根尖细胞造成遗传损伤,但是联合作用时,抗生素在溶液中与Cu发生络合反应,从而可以在一定程度上降低对根尖细胞的遗传损伤.

关键词：抗生素铜蚕豆根尖微核率

固相萃取GC/MS分析血中10种常见农药和杀鼠药

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中国法医学杂志 2009年第4期24卷 269-271页

作者：李文海朱鲁生山东农业大学资环学院山东泰安271018

目的采用固相萃取（SPE）GC／MS分析技术，快速检验血中常见农药及杀鼠药。方法采用SPE-GC／MS法分析血中马拉硫磷、敌敌畏、甲基对硫磷、甲拌磷、灭多威、杀鼠迷、速灭威、久效磷、毒死蜱、灭害威等10种药物；比较OasisHLBcartridge、... 详细信息

目的采用固相萃取（SPE）GC／MS分析技术，快速检验血中常见农药及杀鼠药。方法采用SPE-GC／MS法分析血中马拉硫磷、敌敌畏、甲基对硫磷、甲拌磷、灭多威、杀鼠迷、速灭威、久效磷、毒死蜱、灭害威等10种药物；比较OasisHLBcartridge、C18和C23种固相萃取柱同步提取的回收率及洗脱剂种类、洗脱剂和缓冲液用量对回收率的影响。结果固相萃取柱Oasis HLB cartridge提取率较高，在72．6％-112．0％之间；采用3mL氯仿做洗脱剂时回收率较好，在75．7％～127．2％之间；2mL磷酸盐为缓冲液最佳剂量。结论本文方法具有操作简便快捷，回收率高、重现性好、无杂质干扰等特点，可用于法庭毒物的检验。

关键词：法医毒物分析固相萃取气相色谱/质谱联用仪

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思政元素融入汽车构造(Ⅱ)课程应用研究

时代汽车 2023年第4期 34-36页

作者：朱路生江苏农林职业技术学院江苏省镇江市212400

本文以汽车检测与维修专业的核心课汽车构造(Ⅱ)为例,根据本课程内容特点,提炼与内容相结合的思政元素,并探索将思政元素融入课程教学的思路。课程实施时,采用“课上知行、课后促行和企业践行”三环相融策略展开课程思政。通过课程思政... 详细信息

本文以汽车检测与维修专业的核心课汽车构造(Ⅱ)为例,根据本课程内容特点,提炼与内容相结合的思政元素,并探索将思政元素融入课程教学的思路。课程实施时,采用“课上知行、课后促行和企业践行”三环相融策略展开课程思政。通过课程思政教学的改革,思政元素融入教学效果良好,引领学生在学习专业技能的过程中树立正确的价值观和职业品质。

关键词：专业核心课汽车底盘价值观

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