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Mechanistic analysis for the origin of diverse diterpenes in Tripterygium wilfordii

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Acta Pharmaceutica Sinica B 2022年第6期12卷 2923-2933页

作者： Lichan tu Xinbo Cai Yifeng Zhang Yuru Tong Jian Wang Ping Su Yun Lu Tianyuan Hu Yunfeng Luo Xiaoyi Wu Dan Li luqi Huang Wei Gao Beijing Shijitan Hospital Capital Medical UniversityBeijing 100038China School of Traditional Chinese Medicine Capital Medical UniversityBeijing 100069China School of Medicine Zhejiang University City CollegeHangzhou 310015China School of Pharmaceutical Sciences Capital Medical UniversityBeijing 100069China State Key Laboratory Breeding Base of Dao-di Herbs National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijing 100700China Department of Chemistry The Scripps Research InstituteJupiterFL 33458USA School of Pharmacy Hangzhou Normal UniversityHangzhou 311121China

Tripterygium wilfordii is a valuable medicinal plant rich in biologically active diterpenoids,but there are few studies on the origins of these diterpenoids in its secondary metabolism.Here,we identified three regions containing tandemly duplicated diterpene synthase genes on chromosomes(Chr) 17 and 21 of T. wilfordii and obtained 11 diterpene synthases with different functions.We farther revealed that these diterpene synthases underwent duplication and rearrangement at approximately 2.3-23.7 million years ago(MYA) by whole-genome triplication(WGT),transposon mediation,and tandem duplication,followed by functional divergence.We first demonstrated that four key amino acids in the sequences of TwCPS3,TwCPS5,and TwCPSS were altered during evolution,leading to their functional divergence and the formation of diterpene secondary metabolites.Then,we demonstrated that the functional divergence of three TwKSLs was driven by mutations in two key amino acids.Finally,we discovered the mechanisms of evolution and pseudogenization of miltiradiene synthases in T.wilfordii and elucidated that the new function in TwMS1/2 from the terpene synthase(TPS)-b subfamily was caused by progressive changes in multiple amino acids after the WGT event.Our results provide key evidence for the formation of diverse diterpenoids during the evolution of secondary metabolites in T.wilfordii.

关键词： Tripterygium wilfordii Tandem duplication Diterpene synthases Divergence Secondary metabolism

The chromosome-level reference genome assembly for Panax notoginseng and insights into ginsenoside biosynthesis

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Plant Communications 2021年第1期2卷 54-68页

作者： Zhouqian Jiang Lichan tu Weifei Yang Yifeng Zhang Tianyuan Hu Baowei Ma Yun Lu Xiuming Cui Jie Gao Xiaoyi Wu Yuru Tong Jiawei Zhou Yadi Song Yuan Liu Nan Liu luqi Huang Wei Gao School of Traditional Chinese Medicine Capital Medical UniversityBeijingChina School of Pharmaceutical Sciences Capital Medical UniversityBeijingChina Annoroad Gene Technology BeijingChina Faculty of Life Science and Technology Kunming University of Science and TechnologyKunmingChina State Key Laboratory Breeding Base of Dao-di Herbs National Resource Center for Chinese Materia MedicaChina Academy of Chinese Medical SciencesBeijingChina Advanced Innovation Center for Human Brain Protection Capital Medical UniversityBeijingChina

Panax notoginseng,a perennial herb of the genus Panax in the family Araliaceae,has played an important role in clinical treatment in China for thousands of years because of its extensive pharmacological effects.Here,we report a high-quality reference genome of P.notoginseng,with a genome size up to 2.66 Gb and a contig N50 of 1.12 Mb,produced with third-generation PacBio sequencing technology.This is the first chromosome-level genome assembly for the genus Panax.Through genome evolution analysis,we explored phylogenetic and whole-genome duplication events and examined their impact on saponin biosynthesis.We performed a detailed transcriptional analysis of P.notoginseng and explored genelevel mechanisms that regulate the formation of characteristic tubercles.Next,we studied the biosynthesis and regulation of saponins at temporal and spatial levels.We combined multi-omics data to identify genes that encode key enzymes in the P.notoginseng terpenoid biosynthetic pathway.Finally,we identified five glycosyltransferase genes whose products catalyzed the formation of different ginsenosides in P.notoginseng.The genetic information obtained in this study provides a resource for further exploration of the growth characteristics,cultivation,breeding,and saponin biosynthesis of P.notoginseng.

关键词： chromosome-level genome ginsenoside P.notoginseng regulation transcriptome

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Editorial

Acta Pharmaceutica Sinica B 2017年第2期7卷 118-118,117页

作者： Huang luqi Gao Wei chinese academy of engineering china academy of chinese medical sciences national resource center for chinese materia medica capital medical university

Traditional Chinese medicine and natural products,from which a large proportion of modern drugs in clinical use are derived from,are great resources for special health improvement,economic potential and ecology utilization in China.They play an indispensable role in modern drug R&D.The artemisinin from the Artemisia annua L.,discovered by Nobel Prize winner,Youyou tu,has saved millions of lives and natural medicine has increasingly attracted worldwide attentions.China has a long history

关键词： Editorial

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框架-剪力墙结构弹塑性地震剪力分配分析

东南大学学报（自然科学版） 2023年第6期53卷 1072-1079页

作者：谢鲁齐吴京朱云青黄志挺东南大学混凝土及预应力混凝土结构教育部重点实验室南京211189 东南大学国家预应力工程技术研究中心南京211189 中国电建集团福建省电力勘测设计院有限公司福州350003

基于背景工程,构建考虑协同工作机制的平面分析结构模型,分别对3组不同刚度特征值的框架-剪力墙结构展开参数分析,对框架剪力与结构基底剪力的比值进行定量分析研究,总结弹塑性状态下结构的内力分配机制.针对设计过程中是否对框架剪力... 详细信息

基于背景工程,构建考虑协同工作机制的平面分析结构模型,分别对3组不同刚度特征值的框架-剪力墙结构展开参数分析,对框架剪力与结构基底剪力的比值进行定量分析研究,总结弹塑性状态下结构的内力分配机制.针对设计过程中是否对框架剪力进行调整,建立2类分析模型,探讨了剪力调整对结构配筋、罕遇地震下结构性能以及结构抗倒塌能力的影响.结果表明,现行规范规定的剪力调整对框架-剪力墙抗震性能的影响较小,且调整方案对强柱弱梁的设计原则存在不利影响.结构设计过程中,在保证强柱弱梁和塑性铰转动能力的前提下可不必采取剪力调整措施.

关键词：框架-剪力墙结构弹塑性状态地震剪力分配刚度特征值剪力调整

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中药大健康产业发展机遇与战略思考

中国工程科学 2017年第2期19卷 16-20页

作者：张伯礼张俊华陈士林段金廒黄璐琦孙晓波屠鹏飞叶祖光瞿海斌魏建和赵大庆徐雅娟薛晓娟天津中医药大学天津300193 中国中医科学院北京100700 南京中医药大学南京210023 中国医学科学院北京100193 北京大学北京100871 浙江大学杭州310058 长春中医药大学长春130117 吉林省中医科学院长春130012

随着经济社会发展,人们对健康服务的需求快速增长。中医药是我国独具特色的健康资源,也是潜力巨大的经济资源。中医药健康服务主要包括养生、保健、医疗、康复等,但核心是以中药相关产品为主体的健康服务供给。随着中药现代化进程的推进... 详细信息

随着经济社会发展,人们对健康服务的需求快速增长。中医药是我国独具特色的健康资源,也是潜力巨大的经济资源。中医药健康服务主要包括养生、保健、医疗、康复等,但核心是以中药相关产品为主体的健康服务供给。随着中药现代化进程的推进,也促进了中药大健康产业的悄然形成,形成了涉及中药种植,产品研发、生产、流通、销售在内的跨行业、跨区域的产业链,并具有调整产业结构、增加就业、农民增收、服务医改、惠及民生及保护生态等综合优势。本文介绍了中药大健康产业发展的现状,分析存在的问题和不足,提出了一个战略中心(以服务健康需求为中心),两个战略重点(规划监管、提质增效),四个发展方向(规范种植、新品研发、商贸物流、国际发展)和六项重点任务。

关键词：大健康大健康产业中药中药现代化