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Polysaccharide of Alocasia cucullata Exerts Antitumor Effect by Regulating Bcl-2, Caspase-3 and ERK1/2 Expressions during Long-Time Administration

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Chinese Journal of Integrative Medicine 2024年第1期30卷 52-61页

作者： ZHOU Qi-chun XIAO shi-lin LIN Ru-kun LI Chan CHEN Zhi-jie CHEN Yi-fei luo Chao-hua MO Zhi-xian LIN Ying-bo School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou(510515)China Clinical and Basic Research Team of TCM Prevention and Treatment of Non-Small Cell Lung Cancer the Second Clinical College of Guangzhou University of Chinese MedicineGuangzhou(510120)China Department of Chinese Medicine Zhujiang Hospital Affiliated to Southern Medical UniversityGuangzhou(510282)China Department of Pharmacy Jiangmen Central HospitalJiangmen(529000)China

Objective: To study the in vitro and in vivo antitumor effects of the polysaccharide of Alocasia cucullata(PAC) and the underlying mechanism. Methods: B16F10 and 4T1 cells were cultured with PAC of 40 μg/m L, and PAC was withdrawn after 40 days of administration. The cell viability was detected by cell counting kit-8. The expression of Bcl-2 and Caspase-3 proteins were detected by Western blot and the expressions of ERK1/2 mRNA were detected by quantitative real-time polymerase chain reaction(qRT-PCR). A mouse melanoma model was established to study the effect of PAC during long-time administration. Mice were divided into 3 treatment groups: control group treated with normal saline, positive control group(LNT group) treated with lentinan at 100 mg/(kg·d), and PAC group treated with PAC at 120 mg/(kg·d). The pathological changes of tumor tissues were observed by hematoxylin-eosin staining. The cell apoptosis of tumor tissues was detected by TUNEL staining. Bcl-2 and Caspase-3 protein expressions were detected by immunohistochemistry, and the expressions of ERK1/2, JNK1 and p38 mRNA were detected by qRT-PCR. Results: In vitro, no strong inhibitory effects of PAC were found in various tumor cells after 48 or 72 h of administration. Interestingly however, after 40 days of cultivation under PAC, an inhibitory effect on B16F10 cells was found. Correspondingly, the long-time administration of PAC led to downregulation of Bcl-2 protein(P <0.05), up-regulation of Caspase-3 protein(P<0.05) and ERK1 mRNA(P<0.05) in B16F10 cells. The above results were verified by in vivo experiments. In addition, viability of B16F10 cells under long-time administration culture in vitro decreased after drug withdrawal, and similar results were also observed in 4T1 cells. Conclusions: Long-time administration of PAC can significantly inhibit viability and promote apoptosis of tumor cells, and had obvious antitumor effect in tumor-bearing mice.

关键词： polysaccharide of Alocasia cucullata tumor cells Bcl-2 Caspase-3 ERK1/2 Chinese medicine

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Spatially resolved transcriptomic profiling of placental development in dairy cow

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Zoological Research 2024年第3期45卷 586-600页

作者： Guang-Hui Tan shi-Jie Liu Ming-Le Dou De-Feng Zhao Ao Zhang Heng-Kuan Li Fu-Nong luo Tao shi Hao-Ping Wang Jing-Yuan Lei Yong Zhang Yu Jiang Yi Zheng Fei Wang Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Livestock Biology Northwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Animal Biotechnology of the Ministry of Agriculture Northwest A&F UniversityYanglingShaanxi 712100China College of Information Engineering Northwest A&F UniversityYanglingShaanxi 712100China

The placenta plays a crucial role in successful mammalian reproduction.Ruminant animals possess a semi-invasive placenta characterized by a highly vascularized structure formed by maternal endometrial caruncles and fetal placental cotyledons,essential for full-term fetal development.The cow placenta harbors at least two trophoblast cell populations:uninucleate(UNC)and binucleate(BNC)cells.However,the limited capacity to elucidate the transcriptomic dynamics of the placental natural environment has resulted in a poor understanding of both the molecular and cellular interactions between trophoblast cells and niches,and the molecular mechanisms governing trophoblast differentiation and functionalization.To fill this knowledge gap,we employed Stereo-seq to map spatial gene expression patterns at near single-cell resolution in the cow placenta at 90 and 130 days of gestation,attaining high-resolution,spatially resolved gene expression profiles.Based on clustering and cell marker gene expression analyses,key transcription factors,including YBX1 and NPAS2,were shown to regulate the heterogeneity of trophoblast cell subpopulations.Cell communication and trajectory analysis provided a framework for understanding cell-cell interactions and the differentiation of trophoblasts into BNCs in the placental microenvironment.Differential analysis of cell trajectories identified a set of genes involved in regulation of trophoblast differentiation.Additionally,spatial modules and co-variant genes that help shape specific tissue structures were identified.Together,these findings provide foundational insights into important biological pathways critical to the placental development and function in cows.

关键词： Spatial transcriptomics Dairy cow Placenta Gestation

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Heat Treatment of Cold-Rolled Low-Carbon Si-Mn Dual Phase Steels

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Journal of Iron and Steel Research(International) 2010年第1期17卷 54-58页

作者： luo Juan-juan shi Wen HUANG Qun-fei LI Lin Material Science and Engineering College Shanghai University Shanghai 200072 China

The effects of overageing （OA） and vanadium addition on microstructure and properties of cold-rolled lowcarbon Si-Mn dual phase （DP） steel sheets are studied. The results show that the microstructure and mechanical properties of DP steels are greatly affected by OA temperature. When OA is done at low temperatures （473 -- 573 K）, the elongation rate increases sharply while both the yield strength and tensile strength decrease significantly, When OA is done at high temperatures （〉573 K）, the elongation does not further increase, but the yield strength increases and tensile strength decreases, which deteriorates the mechanical properties of the steel. The results also show that both the strength and the tempering stability can be significantly improved by vanadium microalloying. Finally, the optimum OA temperature for the traditional steel appears to be around 523 K, while it is around 573 K for the V-containing steel, resulting in the best overall mechanical properties.

关键词： dual phase steel overageing microstructure mechanical property vanadium

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Different concentrations of hyaluronic acid eye drops for dry eye syndrome: a systematic review and Meta-analysis

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International Journal of Ophthalmology(English edition) 2024年第6期17卷 1110-1119页

作者： Xiao-Wei Ouyang Sheng Fang Yun-Min Yi shi-Peng Zou Qi-Yu Hu Zi-Xuan Huang Qing-Xia Li Jin-Yan luo Department of Ophthalmology Nanchang Hongdu Hospital of Traditional Chinese MedicineNanchang 330000Jiangxi ProvinceChina Department of Ophthalmology Jiangxi Hospital of Integrated Traditional Chinese and Western MedicineNanchang 330000Jiangxi ProvinceChina Department of Cataract Nanchang University Affiliated Eye HospitalNanchang 330000Jiangxi ProvinceChina Department of Neuro-ophthalmology Nanchang University Affiliated Eye HospitalNanchang 330000Jiangxi ProvinceChina

AIM:To compare high or low concentration of hyaluronic acid eye drops(HY)for dry eye syndromes(DES).METHODS:Randomized controlled trials(RCTs)comparing various concentrations of HY were searched in PubMed,Embase,Web of Science,Cochrane,SinoMed,CNKI,Wanfang Database,CQVIP,and Chinese journals databases between inception and July 2023.Pooled standardized mean differences(SMD)or weighted mean difference(WMD)with 95%confidence intervals(CI)from RCTs evaluating Schirmer’s I test(SIT),corneal fluorescein staining score(CFS),tear breakup time(TBUT),DES score(DESS),and Ocular Surface Disease Index(OSDI)were calculated.Sensitivity analysis,Egger’s test and Meta-regression analysis were performed for all indicators.RESULTS:We conducted a Meta-analysis of 10 RCTs that met the inclusion criteria,involving 1796 cases.High-concentrations group significantly improved the outcome of CFS according to random effects modelling(SMD,-3.37;95%CI,-5.25 to-1.48;P=0.0005).The rest of the results were not statistically significant,including indicators such as SIT,TBUT,DESS and OSDI.CONCLUSION:For dry eyes with positive corneal staining,a high concentration of HY is recommended,whereas in other cases,a high concentration of HY does not offer a more pronounced advantage over a low concentration of HY in the treatment of dry eyes.

关键词： dry eye hyaluronic acid concentration Meta-analysis

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Geological characteristics of unconventional tight oil reservoir (10^(9) t): A case study of Upper Cretaceous Qingshankou Formation, northern Songliao Basin, NE China

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China Geology 2024年第1期7卷 51-62页

作者： Li-zhi shi Zhuo-zhuo Wang Zhan-tao Xing Shan Meng Shuai Guo Si-miao Wu Li-yan luo Chinese Academy of Geological Sciences Beijing 100037China Beijing Institute of Engineering Geology Beijing 100048China

The Daqing exploration area in the northern Songliao Basin has great potential for unconventional oil and gas resources,among which the total resources of tight oil alone exceed 109 t and is regarded as an important resource base of Daqing oilfield.After years of exploration in the Qijia area,Songliao Basin,NE China,tight oil has been found in the Upper Cretaceous Qingshankou Formation.To work out tight oil’s geological characteristics,taking tight oil in Gaotaizi oil layers of the Upper Cretaceous Qingshankou Formation in northern Songliao Basin as an example,this paper systematically analyzed the geological characteristics of unconventional tight oil in Gao3 and Gao4 layers of the Qijia area,based on the data of the geological survey,well drilling journey,well logging,and test.It is that three sets of hydrocarbon source rocks(K2qn1,K2qn2+3,and K2n1)develop in the examined area,and exhibit excellent type I and II kerogens,high organic matter abundance,and moderate maturity.The reservoir is generally composed of thin-bedded mudstone,siltstone,and sandstone,and presents poor porosity(average 8.5 vol.%)and air permeability(average 4 mD).The main reservoir space primarily includes intergranular pores,secondary soluble pores,and intergranular soluble pores.Three types of orifice throats were identified,namely fine throat,extra-fine throat,and micro-fine throat.The siltstone is generally oil-bearing,the reservoirs with slime and calcium become worse oil-bearing,and the mudstone has no obvious oil-bearing characteristics.The brittleness indices of the sandstone in the tight oil reservoir range from 40%to 60%,and those of the mudstone range from 40%to 45%,indicating a better brittleness of the tight oil reservoir.Based on the study of typical core hole data,this paper gives a comprehensive evaluation of the properties of the tight oil and establishes a tight oil single well composite bar chart as well as the initial evaluation system with the core of properties in the tight oil reservoir.This study has theoretical guidi

关键词： Unconventional oil and gas Tight oil Thin-bedded mudstone-siltstone-sandstone reservoir Qijia area Qingshankou Formation Oil and gas exploration engineering Songliao Basin Daqing oilfield

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广西芒果种质资源保存利用和研究概况

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南方园艺 2024年第3期35卷 79-83页

作者：黄国弟李日旺陈永森罗世杏李翔苏美花郭丽梅广西壮族自治区亚热带作物研究所/广西壮族自治区芒果产业绿色高效发展工程研究中心/广西亚热带特色水果质量安全控制重点实验室/农业农村部亚热带果品蔬菜质量安全控制重点实验室广西南宁530001

本文从种质圃建设、种质资源数量和保存方法等方面介绍了广西芒果种质资源收集和保存的总体情况,将广西在芒果种质鉴定评价中取得的主要成果进行总结和显现。从芒果种质资源在常规育种中的利用、分子育种研究两个方面介绍了广西芒果种... 详细信息

本文从种质圃建设、种质资源数量和保存方法等方面介绍了广西芒果种质资源收集和保存的总体情况,将广西在芒果种质鉴定评价中取得的主要成果进行总结和显现。从芒果种质资源在常规育种中的利用、分子育种研究两个方面介绍了广西芒果种质资源利用创新的成果和现状,同时,从全国芒果品种育成情况对广西芒果种质资源保存前景进行了分析。

关键词：芒果种质资源收集保存利用创新研究概况

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Hyperglycemia activates FGFR1 via TLR4/c-Src pathway to induce inflammatory cardiomyopathy in diabetes

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1693-1710页

作者： Xiong Chen Jinfu Qian shiqi Liang Jianchang Qian Wu luo Yujuan shi Hong Zhu Xiang Hu Gaojun Wu Xiaokun Li Guang Liang Department of Endocrinology the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China Chemical Biology Research Center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China Department of Wound Repair the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China Department of Cardiology the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China School of Pharmaceutical Sciences Hangzhou Medical CollegeHangzhou 311399China

Protein tyrosine kinases (RTKs) modulate a wide range of pathophysiological events in several non-malignant disorders, including diabetic complications. To find new targets driving the development of diabetic cardiomyopathy (DCM), we profiled an RTKs phosphorylation array in diabetic mouse hearts and identified increased phosphorylated fibroblast growth factor receptor 1 (p-FGFR1) levels in cardiomyocytes, indicating that FGFR1 may contribute to the pathogenesis of DCM. Using primary cardiomyocytes and H9C2 cell lines, we discovered that high-concentration glucose (HG) transactivates FGFR1 kinase domain through toll-like receptor 4 (TLR4) and c-Src, independent of FGF ligands. Knocking down the levels of either TLR4 or c-Src prevents HG-activated FGFR1 in cardiomyocytes. RNA-sequencing analysis indicates that the elevated FGFR1 activity induces pro-inflammatory responses via MAPKs–NFκB signaling pathway in HG-challenged cardiomyocytes, which further results in fibrosis and hypertrophy. We then generated cardiomyocyte-specific FGFR1 knockout mice and showed that a lack of FGFR1 in cardiomyocytes prevents diabetes-induced cardiac inflammation and preserves cardiac function in mice. Pharmacological inhibition of FGFR1 by a selective inhibitor, AZD4547, also prevents cardiac inflammation, fibrosis, and dysfunction in both type 1 and type 2 diabetic mice. These studies have identified FGFR1 as a new player in driving DCM and support further testing of FGFR1 inhibitors for possible cardioprotective benefits.

关键词： Diabetic cardiomyopathy Protein tyrosine kinases FGFR1 Cardiomyocytes Inflammatory responses Toll-like receptor4 c-Src NFkB

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八卦揉腹法联合中药治疗轻型新型冠状病毒感染

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广东医学 2024年第2期45卷 177-182页

作者：罗士针杨柳艳彭颖原嘉民庞凤舜刘宇黄秋媛邱晓乔颜芳李鉴基广东省中医院中医经典病房广东广州510120 广东省中医院治未病中心广东广州510120 广州中医药大学附属中山中医院肾内科广东中山528400

目的研究八卦揉腹法联合中药治疗轻型新型冠状病毒感染患者的临床疗效。方法采用随机对照的研究方法,选择64例轻型新型冠状病毒感染患者,按随机数字表法分成两组,试验组33例,对照组31例,对照组使用常规中医辨证处理,试验组在常规中医辨... 详细信息

目的研究八卦揉腹法联合中药治疗轻型新型冠状病毒感染患者的临床疗效。方法采用随机对照的研究方法,选择64例轻型新型冠状病毒感染患者,按随机数字表法分成两组,试验组33例,对照组31例,对照组使用常规中医辨证处理,试验组在常规中医辨证基础上,联合使用八卦揉腹法,统计两组核酸转阴时间、退热、咳嗽、腹痛、胃纳、鼻塞、流涕缓解时间,治疗前后评价两组症状积分,同时比较两组治疗结果。结果试验组患者的核酸转阴时间、流涕、咳嗽、恶寒、食欲下降、恶心以及腹胀/腹痛缓解时间均短于对照组(P0.05),治疗后试验组流涕、咳嗽、恶寒、食欲下降、恶心以及腹胀/腹痛积分低于对照组(P<0.05)。结论八卦揉腹法联合中药治疗轻型新型冠状病毒感染患者效果显著,能够缩短核酸转阴时间,促进呼吸道和消化道症状快速缓解,缩短疾病恢复时间。

关键词：八卦揉腹轻型新型冠状病毒感染临床疗效

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Neutrophil-mimicking therapeutic nanoparticles for targeted chemotherapy of pancreatic carcinoma

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Acta Pharmaceutica Sinica B 2019年第3期9卷 575-589页

作者： Xi Cao Ying Hu shi luo Yuejing Wang Tao Gong Xun Sun Yao Fun Zhirong Zhang Key Laboratory of Drug Targeting and Drug Delivery Systems Ministry of EducationWest China School of PharmacySichuan University

Due to the critical correlation between inflammation and carcinogenesis, a therapeutic candidate with anti-inflammatory activity may find application in cancer therapy. Here, we report the therapeutic efficacy of celastrol as a promising candidate compound for treatment of pancreatic carcinoma via na?ve neutrophil membrane-coated poly(ethylene glycol) methyl ether-block-poly(lactic-co-glycolic acid)(PEG-PLGA) nanoparticles. Neutrophil membranecoated nanoparticles(NNPs) are well demonstrated to overcome the blood pancreas barrier to achieve pancreas-specific drug delivery in vivo. Using tumor-bearing mice xenograft model, NNPs showed selective accumulations at the tumor site following systemic administration as compared to nanoparticles without neutrophil membrane coating. In both orthotopic and ectopic tumor models,celastrol-loaded NNPs demonstrated greatly enhanced tumor inhibition which significantly prolonged the survival of tumor bearing mice and minimizing liver metastases. Overall, these results suggest that celastrol-loaded NNPs represent a viable and effective treatment option for pancreatic carcinoma.

关键词： Na■ve neutrophils membrane Celastrol PEG-PLGA nanoparticle Pancreatic carcinoma Inflammation

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Analysis of Epidemiological and Issues Encountered in Case Reports on Echinococcosis—China,2022

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China CDC weekly 2024年第6期6卷 100-104页

作者： Yan Kui Shuai Han Xiaojuan Zhang Guangzhong shi Yale Yu Ruirui Li Peizheng Zhan Hongju Duan Wenbo luo Benfu Li Shaoqi Ning Yalan Zhang Aiya Zhu Jingbo Xue National Institute of Parasitic Diseases Chinese Center for Disease Control and Prevention(Chinese Center for Tropical Diseases Research)National Health Commission Key Laboratory of Parasite and Vector BiologyWorld Health Organization Collaborating Center for Tropical DiseasesNational Center for International Research on Tropical DiseasesShanghaiChina Gansu Provincial Center for Disease Control and Prevention Lanzhou CityGansu ProvinceChina The Center for Disease Control and Prevention of Xinjiang Uygur Autonomous Region Urumuqi CityXinjiang Uygur Autonomous RegionChina Xinjiang Production and Construction Corps Center for Disease Control and Prevention Urumuqi CityXinjiang Uygur Autonomous RegionChina Sichuan Provincial Center for Disease Control and Prevention Chengdu CitySichuan ProvinceChina Qinhai Institute for Endemic Disease Control and Prevention Xining CityQinghai ProvinceChina Ningxia Center for Disease Control and Prevention Yinchuan CityNingxia Hui Autonomous RegionChina Inner Mongolia Autonomous Region Center for Comprehensive Disease Control and Prevention Hohhot CityInner Mongolia AutonomousChina Yunnan Institute of Parasitic Diseases Puer CityYunnan ProvinceChina Shaanxi Provincial Center for Disease Control and Prevention Xi'an CityShaanxi ProvinceChina Henan Provincial Center for Disease Control and Prevention Zhengzhou CityHenan ProvinceChina Guizhou Provincial Center for Disease Control and Prevention Guiyang CityGuizhou ProvinceChina

Summary What is already known about this topic?Echinococcosis is classified as a Class C infectious disease in China.It is endemic in 370 counties located in the agricultural and pastoral regions of western China.What is added by this report?This report provides a comprehensive overview of the cases of echinococcosis reported in China in 2022.Following a thorough evaluation conducted by provincial CDCs.

关键词： China Ech counties

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