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Therapeutic effects of the extract of Sancao Formula, a Chinese herbal compound, on imiquimod-induced psoriasis via cysteine-rich protein 61

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Journal of Integrative Medicine 2022年第4期20卷 376-384页

作者： Wan-jun Guo Yi Wang Yu deng Lin-yan Cheng Xin Liu Ruo-fan Xi Sheng-jie Zhu Xin-yi Feng Liang Hua Kan Ze Jian-yong Zhu Dong-jie Guo Fu-lun Li Department of Dermatology Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China School of Medicine Chengdu UniversityChengdu 610106Sichuan ProvinceChina Institute of Cancer Biology and Drug Discovery Chengdu UniversityChengdu 610106Sichuan ProvinceChina Clinical Laboratory Medicine Center Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China

Objective: Psoriasis is a common chronic inflammatory skin disease that is prone to recurrence, and the proinflammatory factor, cysteine-rich protein 61(Cyr61), is important in its pathophysiology. Long-term clinical practice has shown that Sancao Formula(SC), a Chinese herbal compound, is effective in the treatment of psoriasis, but the precise mechanism remains unknown. In this study, we investigate the mechanism by which SC extract alleviates imiquimod(IMQ)-induced ***: The expression of Cyr61 in psoriatic lesions and normal healthy skin was detected using immunohistochemical analysis to investigate the biological role of Cyr61 in models of psoriatic inflammation. A psoriatic mouse model was established by topical application of IMQ, and the effect of topical application of SC extract was evaluated using the psoriasis area and severity index(PASI) score,hematoxylin-eosin staining, and histopathological features of the skin. Next, a HaCaT cell inflammation model was established using interferon-γ(IFN-γ), and the effect of SC extract on the mRNA and protein levels of Cyr61 and intercellular cell adhesion molecule-1(ICAM-1) was confirmed using Western blot and quantitative real-time polymerase chain reaction ***: Immunohistochemical staining showed that the expression of Cyr61 in psoriatic lesions was higher than that in normal skin samples(78.26% vs 41.18%, P < 0.05), and the number of Cyr61-positive cells in psoriatic lesions was also significantly higher than in normal skin(18.66 ± 2.51 vs4.33 ± 1.52, P < 0.05). Treatment in mice with IMQ-induced psoriasis showed that SC extract could significantly improve the inflammatory phenotype, PASI score(10.875 ± 0.744 vs 3.875 ± 0.582, P < 0.05),and pathological features compared with those in IMQ model group;SC treatment was also associated with decreased levels of Cyr61 and ICAM-1. In the IFN-γ-induced inflammatory cell model, the mRNA and protein levels of Cyr61 and ICAM-1 were upregulated, w

关键词： Traditional Chinese medicine Sancao Formula Psoriasis Plant extracts Cysteine-rich protein 61 Intercellular cell adhesion molecule-1

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Research advances in phytochemistry,pharmacology and toxicology of oleanolic acid

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中国药理学与毒理学杂志 2021年第10期35卷 770-771页

作者： REN shan SUN Qiang CHEN Li ZENG sha ZHAO Hui LIU Mao-lun YANG Han MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

Oleanolic acid(OA)is a pentacyclic triterpenoid chemical component that exists in natural plants with a molecular formula of C30H48O3 and a molecular weight at 456.71 g·*** is widespread in traditional Chinese herbal medicine(Ligustri Lucidi Fructus,Achyranthis Bidentate Radix,Red Sage)and berries(blueberries,grapes).In recent years,because of the extensive pharmacological effects of OA,its advantages in disease treatment have become increasingly prominent and gradually attracted the attention of pharmaceutical *** has effective therapeutic effects on a series of chronic diseases such as inflammation,cancer,diabetes,and cardiovascular diseases through multiple signaling pathways and various *** in cancers,such as colorectal cancer,liver cancer,gastric cancer,lung cancer,breast cancer and other malignancies,OA presents substantial ***,its poor aqueous solubility,needy bioavailability,and unsatisfactory pharmacological activity excessively restrict its clinical *** importantly,the improper utilization of OA can cause adverse reactions,toxic effects and even damage to organs in some specific *** the discovery of various pharmacological effects,the complex action mechanisms of OA,the continuous progress in structural modification of OA,as well as the synthesis of OA derivatives,its application is expanding *** numerous studies,there is a clear indication that OA and its derivatives,if fully developed,may provide an alternative and cheaper treatment for a variety of chronic ***,the specific molecular mechanisms of OA and its derivatives as an alternative therapy and supplementary therapy for cancer,diabetes,cardiovascular disease and other chronic diseases remain to be ***,it is necessary to further study the pharmacokinetics,pharmacological activity,specific targets and related mechanisms of OA to lay a solid foundation for drug development and the application o

关键词： oleanolic acid pharmacology toxicology derivatives review

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Scutellarin induces apoptosis of colorectal cancer HCT116 cells via Hippo signaling pathway

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中国药理学与毒理学杂志 2021年第10期35卷 775-776页

作者： YANG Han SUN Qiang ZENG sha CHEN Li ZHAO Hui LIU Mao-lun REN shan MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To investigate the effect of scutellarin on the apoptosis of human colorectal cancer cells via the Hippo signaling pathway in *** MTT colorimetric method was used to detect the influence of scutellarin on the survival rate of HCT116 *** the effect of scutellarin at various concentrations on cell morphology was observed by *** scratch experiment was used to detect the influence of scutellarin on the migration of HCT116 ***33342/PI double staining method was used to detect the effect of scutellarin on the apoptosis of HCT116 *** blotting method was used to assess the action of scutellarin on the expressions of Hippo signaling pathway-related proteins Mst1,Lats1,YAP1,p-YAP(Ser127),TAZ,and its downstream effector proteins c-Myc and cyclin D1,as well as apoptosis-related proteins Bcl-2 and Bax in HCT116 *** Scutellarin significantly affected the morphology of HCT116 cells and reduced the survival rate of HCT116 ***33342/PI double staining showed that scutellarin effectively induced the apoptosis of HCT116 *** blotting analysis showed that the expression levels of Hippo signaling pathway-related proteins Mst1,Lats1,YAP1,TAZ and its downstream effector proteins c-Myc,cyclin D1 were down-regulated in a concentration-dependent manner by scutellarin,and the expression of p-YAP(ser127)was ***,scutellarin substantially lessened the expression level of apoptosis-related protein Bcl-2,and promoted the protein level of *** Scutellarin may inhibit the proliferation and migration of HCT116 cells,while induce its apoptosis,potentially by activation of Hippo signaling pathway.

关键词： scutellarin colorectal cancer HCT116 cells Hippo signaling pathway

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Inhibition of colorectal cancer by ursolic acid via noncanonical Hedgehog signaling pathway

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中国药理学与毒理学杂志 2021年第10期35卷 759-760页

作者： CHEN Li SUN Qiang ZENG sha ZHAO Hui LIU Mao-lun YANG Han REN shan MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To identify the inhibitory effect of ursolic acid on the colorectal cancer HCT116 cells in vitro and in vivo,and to explore the underlying *** The smoothened(SMO)gene-silenced human colorectal cancer HCT116^(hSMO)cell line was established by transfection with the lentivirus carrying SMO *** cytotoxic effect of ursolic acid on HCT116^(hSMO)cells was determined by MTT *** effect of ursolic acid on the migration of HCT116^(hSMO)cells was studied by wound healing *** effect of ursolic acid on apoptosis of HCT116^(hSMO)cells was explored by Hoechst33342/PI double staining and flow *** effects of ursolic acid on the expressions of apoptotic marker gene Bcl-2,Bax,caspase-3 and caspase-9 were measured by real-time quantitative RT-PCR(RT-qPCR)and Western blotting(WB)***-qPCR and WB were used to examine the relationship between GLI1,c-Myc expression and PI3K/Akt pathway to further investigate the mechanism of GLI1 activation in HCT116^(hSMO)*** effects of ursolic acid on the expressions of GLI1,p-Akt,Akt,c-Myc,SHH and SUFU of noncanonical Hedgehog pathway were evaluated by RT-qPCR and WB *** nude mouse model bearing HCT116^(hSMO)cells was established and intraperitoneally treated with ursolic acid to investigate the effect on tumor growth in *** body weight and tumor size of mice were assessed regularly every 2 *** effect of ursolic acid on the apoptosis of tumor tissue was determined by TUNEL *** expressions of Bcl-2,Bax,GLI1,p-Akt,Akt,c-Myc,SHH,SUFU mRNA and proteins were measured by RT-qPCR and *** levels of Bcl-2,Bax,GLI1,p-Akt,c-Myc and SHH proteins in tumor tissues were also evaluated by *** Ursolic acid significantly inhibited the growth and migration of HCT116^(hSMO)cells in vitro,compared with the control(P<0.05).Meanwhile,ursolic acid also induced apoptosis of HCT116^(hSMO)cells in vitro(P<0.05).Furthermore,SC79(Akt activator)enhanced the expressio

关键词： ursolic acid colorectal cancer noncanonical Hedgehog signaling apoptosis

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Inhibitory action of berberine on colorectal cancer HCT116 cells by regulation of Hedgehog signaling pathway

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中国药理学与毒理学杂志 2021年第10期35卷 771-772页

作者： SUN Qiang CHEN Li ZENG sha LIU Mao-lun REN shan ZHAO Hui YANG Han MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To investigate the inhibition and mechanism of berberine on human colorectal cancer HCT116 cells through canonical Hedgehog signaling *** The effect of berberine on cell morphology was observed by *** colorimetric assay,cell scratch experiment,colony formation assay and Hoechest/PI staining were utilized to detect the activities of berberine on cell viability,cell migration and cell *** cytometry was applied to examine the cell *** effects of berberine on caspase-3 and caspase-9 were detected by caspase activity detection *** expressions of Hedgehog signaling pathway-related proteins SHH,GLI1,PTCH1,SMO,SUFU,apoptosis-related proteins Bax and Bcl-2 as well as cell cycle-related proteins cyclin D1 were detected by Western ***,quantitative real time RT-PCR was employed to assess the mRNA expression levels of Hedgehog signaling pathway-related genes SHH,GLI1,PTCH1,SMO,SUFU,apoptosis-related genes Bax and Bcl-2 as well as cell cycle-related genes cyclin *** Berberine sharply altered the morphology of human colorectal cancer HCT116 cells,demonstrated by that migration ability of HCT116 cells was reduced significantly and the nuclei were densely *** could induce apoptosis in a dose-dependent *** activities of caspase-3 and caspase-9 were increased *** expression levels of Hedgehog signaling pathway-related protein SUFU and apoptosis-related protein Bax were augmented *** expression levels of Hedgehog signaling pathway-related proteins SHH,GLI1,PTCH1,SMO,apoptosis-related protein Bcl-2 as well as cell cycle-related genes cyclin D1 were markedly ***,the mRNA expression levels of Hedgehog signaling pathway-related gene SUFU and apoptosis-related gene Bax were augmented *** mRNA expression levels of Hedgehog signaling pathway-related genes SHH,GLI1,PTCH1,SMO,apoptosis-related gene Bcl-2 as well as cell cycle-related gene c

关键词： berberine Hedgehog signaling pathway colorectal cancer cell proliferation cell apoptosis

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女性尿失禁生活质量测评量表的研究进展

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中国全科医学 2017年第23期20卷 2934-2938页

作者：王莎邓琛中南大学湘雅三医院普外一科31病室湖南省长沙市410013

女性尿失禁的患病率日益增高,患者的生活质量受到不同程度的影响。本文就国内外女性尿失禁患者生活质量测评工具的相关研究进行综述,重点描述了特异性量表,旨在为进一步发展适合我国女性尿失禁患者生活质量测评量表及为研究者选择合适... 详细信息

女性尿失禁的患病率日益增高,患者的生活质量受到不同程度的影响。本文就国内外女性尿失禁患者生活质量测评工具的相关研究进行综述,重点描述了特异性量表,旨在为进一步发展适合我国女性尿失禁患者生活质量测评量表及为研究者选择合适的测评工具提供参考。

关键词：尿失禁生活质量量表

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RF design of a compact 648 MHz single spoke cavity at CSNS

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Radiation Detection Technology and Methods 2019年第3期3卷 301-306页

作者： Yun Wang Huachang Liu Jianping Dai Peng sha Ziwei deng Bo Li Mengxu Fan Ahong Li Peihua Qu Xiaolei Wu Qiang Chen Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Spallation Neutron Source Science Center Dongguan 523803China University of Chinese Academy of Sciences Beijing 100049China

Purpose In order to increase CSNS beam power from 100 to 500 kW,the linac injection energy needs to be increased from 80 to 300 *** combined layout of superconducting spoke cavities and lliptical cavities will be adopted to accelerate H-beam to 300 *** this paper,a compact single spoke cavity withφ50-mm beam aperture was proposed,with the RF performance of the spoke cavity optimized to E_(p)/E_(acc).shape modification was made to get a reasonable*** The simulation got optimum results of E_(peak)/E_(acc)=4.97 and B_(peak)/E_((acc)=8.42 mT/(MV/m),and themax is 1.52at E_(acc)=7.74 MV/*** A 648 MHzβ=0.4 single spoke cavity with beam apertureФ50 mm was proposed in the *** compact cavity may be a very promising option for CSNS linac *** addition to the optimization of the RF parameters,the multipacting behavior of the cavity is also studied,and cavity shape is optimized to reducevalue.

关键词： Single spoke cavity RF performance optimization Multipacting simuaion

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Increased Chondrocyte Apoptosis in Kashin-Beck Disease and Rats Induced by T-2 Toxin and Selenium Deficiency

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Biomedical and Environmental Sciences 2017年第5期30卷 351-362页

作者： YANG Hao Jie ZHANG Ying WANG Zhi lun XUE Sen Hai LI Si Yuan ZHOU Xiao Rong ZHANG Meng FANG Qian WANG Wen Jun CHEN Chen deng Xiang Hua CHEN Jing Hong Institute of Endemic Diseases School of Public HealthXi'an Jiaotong University Health Science CenterKey Laboratory of Trace Elements and Endemic DiseasesNational Health and Family Planning CommissionXi'an 710061ShaanxL China Xi'an Jiaotong University Hospital Xi'an 710049ShaanxL China School of Biomedical Sciences the University of QueenslandBrisbane 4072Australia Laboratory for Soft Tissue Research Hospital for Special SurgeryNew York 10021USA

Objective To investigate chondrocyte apoptosis and the expression of biochemical markers associated with apoptosis in Kashin-Beck disease（KBD） and in an established T-2 toxin-and selenium（Se） deficiency-induced rat model. Methods Cartilages were collected from the hand phalanges of five patients with KBD and five healthy children. Sprague-Dawley rats were administered a selenium-deficient diet for 4 weeks prior to T-2 toxin exposure. The apoptotic chondrocytes were observed by terminal deoxynucleotidyl transferase d UTP nick end labeling staining. Caspase-3, p53, Bcl-2, and Bax proteins in the cartilages were visualized by immunohistochemistry, their protein levels were determined by Western blotting, and m RNA levels were determined by real-time reverse transcription polymerase chain reaction. Results Increased chondrocyte apoptosis was observed in the cartilages of children with KBD. Increased apoptotic and caspase-3-stained cells were observed in the cartilages of rats fed with normal and Se-deficient diets plus T-2 toxin exposure compared to those in rats fed with normal and Se-deficient diets. Caspase-3, p53, and Bax proteins and m RNA levels were higher, whereas Bcl-2 levels were lower in rats fed with normal or Se-deficiency diets supplemented with T-2 toxin than the corresponding levels in rats fed with normal diet. Conclusion T-2 toxin under a selenium-deficient nutritional status induces chondrocyte death, which emphasizes the role of chondrocyte apoptosis in cartilage damage and progression of KBD.

关键词： KBD Chondrocyte Apoptosis T-2 toxin Selenium-deficiency

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Ring artifacts correction based on the projection-field in neutron CT

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Chinese Physics B 2021年第5期30卷 267-271页

作者： Sheng-Xiang Wang Jie Chen Zhi-Jian Tan Si-Hao deng Yao-Da Wu Huai-Le Lu Shou-Ding Li Wei-Chang Chen lun-Hua He Spallation Neutron Source Science Center Dongguan 523803China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Institute of Physics Chinese Academy of SciencesBeijing 100190China Key Laboratory of shale Gas and Geoengineering Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China Innovation Academy for Earth Science Chinese Academy of SciencesBeijing 100029China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 100049China

Ring artifacts will happen mostly when the detector has inconsistent response among the detector channels,and the characteristic produced rings centered in the iso-center in the reconstructed slices inevitably affect the recognition and analysis of the corresponding sample structures in neutron computed tomography(CT).In this work,a ring correction method based on the projection-field(RCP)is proposed,it is a pre-processing method and provides the corrected projection data directly,which is also conducive to efficient data storage and other algorithmic *** and physical experiments are performed for verifying the effect of the method,and one of the correction methods based on the image-field is used for *** results demonstrate that the RCP can correct the ring artifacts well without reducing the image resolution or over-correction.

关键词： ring artifacts correction neutron CT projection field

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共和盆地沙化土地青杨林恢复规律及影响因素

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西北林学院学报 2023年第5期38卷 117-124,169页

作者：王伦邓磊青海大学农林科学院青海西宁810016 青海省林业和草原项目服务中心青海西宁810000 武威市人才交流开发服务中心甘肃武威733099

比较青海高寒区不同恢复年限沙化土地青杨林恢复效果,为沙化土地人工林持续发育及脆弱生境生态修复提供理论依据。以共和盆地东缘贵南县黄沙头青杨群落为研究对象,对丘间低地造林后5、10、15、20 a和25 a的人工建群种林分结构、群落组... 详细信息

比较青海高寒区不同恢复年限沙化土地青杨林恢复效果,为沙化土地人工林持续发育及脆弱生境生态修复提供理论依据。以共和盆地东缘贵南县黄沙头青杨群落为研究对象,对丘间低地造林后5、10、15、20 a和25 a的人工建群种林分结构、群落组成、植物和微生物细菌多样性、土壤理化性质进行对比分析。结果表明,1)共和盆地沙化土地青杨人工林经过25 a发育,林木衰退明显,径级结构和高度级结构表明,25 a青杨林生长滞后,林下无更新,不具备持续发育的潜力。2)随着造林时间的推移,灌木层Simpson、shannon-wiener、Pielou指数、土壤细菌丰富度及多样性指数先增大后减小;草本层Simpson先减小后增大,shannon-wiener减小。3)随着恢复年限的增加,土壤养分水分可以达到比较稳定状态,但在20 a后增加不明显。4)冗余分析(RDA)表明,速效N、土壤含水率是影响共和盆地人工林土壤细菌结构多样性和林分特征的主要环境因子。未来该区域人工林植被恢复中,应尽量避免青杨纯林的营造,现有青杨林应该通过引进乡土乔灌草,增加群落丰富度及多样性,完善群落复层结构,抚育改造成稀疏混交林,提高生态防护效益。

关键词：共和盆地青杨林不同恢复年限群落结构物种多样性

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