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Suppression of AOM/DSS-induced colorectal cancer by scutellarin through downregulation of Wnt signaling pathway activity

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中国药理学与毒理学杂志 2021年第10期35卷 739-740页

作者： ZENG Sha ZHAO Hui CHEN Li SUN Qiang REN Shan LIU mao-lun YANG Han TANG Shun LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To investigate the therapeutic effect of scutellarin on colitis-associated cancer(CAC)and its underlying mechanism based on Wnt/β-catenin signaling *** The mouse model of CAC was established by azomethane oxide(AOM)and sodium dextran sulfate(DSS),followed by scutellarin treatment,with recording the body weight,diarrhea and *** sacrificing the mice,the colorectal length and colorectal tumor were *** levels of pro-inflammatory factors TNF-αand IL-6 in mice′s sera were measured by the enzyme-linked immunosorbent assay(ELISA).The colorectal lesions were appraised by hematoxylin and eosin(H&E)***β-catenin level in CAC tissues was probed by immunofluorescent *** apoptosis-related genes Bax and Bcl-2,and Wnt signaling pathway-related genesβ-catenin,GSK-3β,TCF4,c-Myc and cyclin D1 were detected by real-time quantitative RT-PCR(RT-qPCR).Finally,Western blotting analysis(WB)was employed to examine the expressions of the apoptosis and Wnt signaling pathway-related *** Scutellarin significantly improved AOM/DSS-caused weight loss,colorectal length shortening,and tumor growth in mice(P<0.01).Meanwhile,colorectal lesions could be substantially alleviated by *** results showed that the levels of pro-inflammatory factors TNF-αand IL-6 were drastically lessened(P<0.01).Scutellarin also sharply inhibited the nuclear translocation ofβ-catenin,as evidenced by the reduction in the nuclear level ofβ-catenin *** addition,scutellarin attenuated the mRNA expression of Wnt signaling pathway-relatedβ-catenin,TCF4,c-Myc and cyclin D1,whereas it heightened GSK-3βmRNA *** results were consolidated by WB analysis,which indicated that scutellarin could mitigate the protein levels of phospho-GSK-3β,β-catenin,TCF4,c-Myc and cyclin D1,with the increase in GSK-3βprotein in CAC ***,scutellarin could induce the apoptosis of CAC,demonstrated by enhanced expression of Bax and diminished ex

关键词： scutellarin colorectal cancer Wnt signaling pathway AOM/DSS

Inhibition of colorectal cancer by ursolic acid via noncanonical Hedgehog signaling pathway

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中国药理学与毒理学杂志 2021年第10期35卷 759-760页

作者： CHEN Li SUN Qiang ZENG Sha ZHAO Hui LIU mao-lun YANG Han REN Shan MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To identify the inhibitory effect of ursolic acid on the colorectal cancer HCT116 cells in vitro and in vivo,and to explore the underlying *** The smoothened(SMO)gene-silenced human colorectal cancer HCT116^(hSMO)cell line was established by transfection with the lentivirus carrying SMO *** cytotoxic effect of ursolic acid on HCT116^(hSMO)cells was determined by MTT *** effect of ursolic acid on the migration of HCT116^(hSMO)cells was studied by wound healing *** effect of ursolic acid on apoptosis of HCT116^(hSMO)cells was explored by Hoechst33342/PI double staining and flow *** effects of ursolic acid on the expressions of apoptotic marker gene Bcl-2,Bax,caspase-3 and caspase-9 were measured by real-time quantitative RT-PCR(RT-qPCR)and Western blotting(WB)***-qPCR and WB were used to examine the relationship between GLI1,c-Myc expression and PI3K/Akt pathway to further investigate the mechanism of GLI1 activation in HCT116^(hSMO)*** effects of ursolic acid on the expressions of GLI1,p-Akt,Akt,c-Myc,SHH and SUFU of noncanonical Hedgehog pathway were evaluated by RT-qPCR and WB *** nude mouse model bearing HCT116^(hSMO)cells was established and intraperitoneally treated with ursolic acid to investigate the effect on tumor growth in *** body weight and tumor size of mice were assessed regularly every 2 *** effect of ursolic acid on the apoptosis of tumor tissue was determined by TUNEL *** expressions of Bcl-2,Bax,GLI1,p-Akt,Akt,c-Myc,SHH,SUFU mRNA and proteins were measured by RT-qPCR and *** levels of Bcl-2,Bax,GLI1,p-Akt,c-Myc and SHH proteins in tumor tissues were also evaluated by *** Ursolic acid significantly inhibited the growth and migration of HCT116^(hSMO)cells in vitro,compared with the control(P<0.05).Meanwhile,ursolic acid also induced apoptosis of HCT116^(hSMO)cells in vitro(P<0.05).Furthermore,SC79(Akt activator)enhanced the expressio

关键词： ursolic acid colorectal cancer noncanonical Hedgehog signaling apoptosis

Inhibitory action of berberine on colorectal cancer HCT116 cells by regulation of Hedgehog signaling pathway

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中国药理学与毒理学杂志 2021年第10期35卷 771-772页

作者： SUN Qiang CHEN Li ZENG Sha LIU mao-lun REN Shan ZHAO Hui YANG Han MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To investigate the inhibition and mechanism of berberine on human colorectal cancer HCT116 cells through canonical Hedgehog signaling *** The effect of berberine on cell morphology was observed by *** colorimetric assay,cell scratch experiment,colony formation assay and Hoechest/PI staining were utilized to detect the activities of berberine on cell viability,cell migration and cell *** cytometry was applied to examine the cell *** effects of berberine on caspase-3 and caspase-9 were detected by caspase activity detection *** expressions of Hedgehog signaling pathway-related proteins SHH,GLI1,PTCH1,SMO,SUFU,apoptosis-related proteins Bax and Bcl-2 as well as cell cycle-related proteins cyclin D1 were detected by Western ***,quantitative real time RT-PCR was employed to assess the mRNA expression levels of Hedgehog signaling pathway-related genes SHH,GLI1,PTCH1,SMO,SUFU,apoptosis-related genes Bax and Bcl-2 as well as cell cycle-related genes cyclin *** Berberine sharply altered the morphology of human colorectal cancer HCT116 cells,demonstrated by that migration ability of HCT116 cells was reduced significantly and the nuclei were densely *** could induce apoptosis in a dose-dependent *** activities of caspase-3 and caspase-9 were increased *** expression levels of Hedgehog signaling pathway-related protein SUFU and apoptosis-related protein Bax were augmented *** expression levels of Hedgehog signaling pathway-related proteins SHH,GLI1,PTCH1,SMO,apoptosis-related protein Bcl-2 as well as cell cycle-related genes cyclin D1 were markedly ***,the mRNA expression levels of Hedgehog signaling pathway-related gene SUFU and apoptosis-related gene Bax were augmented *** mRNA expression levels of Hedgehog signaling pathway-related genes SHH,GLI1,PTCH1,SMO,apoptosis-related gene Bcl-2 as well as cell cycle-related gene c

关键词： berberine Hedgehog signaling pathway colorectal cancer cell proliferation cell apoptosis

Research advances in phytochemistry,pharmacology and toxicology of oleanolic acid

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中国药理学与毒理学杂志 2021年第10期35卷 770-771页

作者： REN Shan SUN Qiang CHEN Li ZENG Sha ZHAO Hui LIU mao-lun YANG Han MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

Oleanolic acid(OA)is a pentacyclic triterpenoid chemical component that exists in natural plants with a molecular formula of C30H48O3 and a molecular weight at 456.71 g·*** is widespread in traditional Chinese herbal medicine(Ligustri Lucidi Fructus,Achyranthis Bidentate Radix,Red Sage)and berries(blueberries,grapes).In recent years,because of the extensive pharmacological effects of OA,its advantages in disease treatment have become increasingly prominent and gradually attracted the attention of pharmaceutical *** has effective therapeutic effects on a series of chronic diseases such as inflammation,cancer,diabetes,and cardiovascular diseases through multiple signaling pathways and various *** in cancers,such as colorectal cancer,liver cancer,gastric cancer,lung cancer,breast cancer and other malignancies,OA presents substantial ***,its poor aqueous solubility,needy bioavailability,and unsatisfactory pharmacological activity excessively restrict its clinical *** importantly,the improper utilization of OA can cause adverse reactions,toxic effects and even damage to organs in some specific *** the discovery of various pharmacological effects,the complex action mechanisms of OA,the continuous progress in structural modification of OA,as well as the synthesis of OA derivatives,its application is expanding *** numerous studies,there is a clear indication that OA and its derivatives,if fully developed,may provide an alternative and cheaper treatment for a variety of chronic ***,the specific molecular mechanisms of OA and its derivatives as an alternative therapy and supplementary therapy for cancer,diabetes,cardiovascular disease and other chronic diseases remain to be ***,it is necessary to further study the pharmacokinetics,pharmacological activity,specific targets and related mechanisms of OA to lay a solid foundation for drug development and the application o

关键词： oleanolic acid pharmacology toxicology derivatives review

Scutellarin induces apoptosis of colorectal cancer HCT116 cells via Hippo signaling pathway

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中国药理学与毒理学杂志 2021年第10期35卷 775-776页

作者： YANG Han SUN Qiang ZENG Sha CHEN Li ZHAO Hui LIU mao-lun REN Shan MING Tian-qi LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To investigate the effect of scutellarin on the apoptosis of human colorectal cancer cells via the Hippo signaling pathway in *** MTT colorimetric method was used to detect the influence of scutellarin on the survival rate of HCT116 *** the effect of scutellarin at various concentrations on cell morphology was observed by *** scratch experiment was used to detect the influence of scutellarin on the migration of HCT116 ***33342/PI double staining method was used to detect the effect of scutellarin on the apoptosis of HCT116 *** blotting method was used to assess the action of scutellarin on the expressions of Hippo signaling pathway-related proteins Mst1,Lats1,YAP1,p-YAP(Ser127),TAZ,and its downstream effector proteins c-Myc and cyclin D1,as well as apoptosis-related proteins Bcl-2 and Bax in HCT116 *** Scutellarin significantly affected the morphology of HCT116 cells and reduced the survival rate of HCT116 ***33342/PI double staining showed that scutellarin effectively induced the apoptosis of HCT116 *** blotting analysis showed that the expression levels of Hippo signaling pathway-related proteins Mst1,Lats1,YAP1,TAZ and its downstream effector proteins c-Myc,cyclin D1 were down-regulated in a concentration-dependent manner by scutellarin,and the expression of p-YAP(ser127)was ***,scutellarin substantially lessened the expression level of apoptosis-related protein Bcl-2,and promoted the protein level of *** Scutellarin may inhibit the proliferation and migration of HCT116 cells,while induce its apoptosis,potentially by activation of Hippo signaling pathway.

关键词： scutellarin colorectal cancer HCT116 cells Hippo signaling pathway

Ursolic acid ameliorates azoxymethane/dextran sulfate sodium-caused colorectal cancer by inhibition of Wnt signaling cascade

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中国药理学与毒理学杂志 2021年第10期35卷 780-781页

作者： ZHAO Hui SUN Qiang ZENG Sha CHEN Li LIU mao-lun REN Shan YANG Han MING Tian-qi TAO Qiu LU Jin-jian XU Hai-bo State Key Laboratory of Southwestern Chinese Medicine Resources Department of PharmacologyChengdu University of Traditional Chinese MedicineChengdu 611137China Institute of Chinese Medical Sciences University of MacaoMacaoChina

OBJECTIVE To investigate the pharmacological effect of ursolic acid(UA)on colitis-associated colorectal cancer(CAC)and its underlying mechanism based on the Wnt signaling *** The CAC model in mice was established by azoxymethane(AOM)combined and dextran sulfate sodium salt(DSS),accompanied by treatment with various dosages of UA and concomitant appraisal of body weight,stool and physical state of the *** the sacrifice of the mice,the tumor and length of the colorectum were measured,followed by retrieval of the liver,spleen,thymus and tumor tissue for downstream *** levels of inflammatory factors interleukin-6(IL-6),IL^(-1)βand C-reactive protein(CRP)in the tumor and serum were examined by enzyme-linked immunosorbent assay(ELISA).The pathological changes of colorectal tissues were observed by HE *** levels in tumors of Wnt/β-catenin signaling pathway-related proteins Wnt4,GSK-3β,β-catenin,TCF4,LEF1,c-Myc,cyclin D1 and apoptosis-related protein Bcl-2 were assayed by immunohistochemistry(IHC).The mRNA expressions of Wnt4,GSK-3β,β-catenin,TCF4,LEF1,c-Myc,cyclin D1,Bcl-2,Bax,caspase-9 and caspase-3 in tumors were detected by real-time quantitative RT-PCR(RT-qPCR).The protein levels of Wnt4,GSK-3β,β-catenin,TCF4,LEF1,c-Myc,cyclin D1,phospho-β-catenin,phospho-GSK-3β,Bcl-2 and Bax in tumors were probed by analyzed by Western blotting(WB).Also,RNA-seq was employed to assess the gut microbiota in the *** UA significantly ameliorated the symptoms of AOM/DSS-induced mouse CAC,evidenced by improved physical state,body weight,survival rate,colorectal length,the mass of liver,thymus,spleen,and decreased CAC load and colorectal *** attenuated the levels of IL-6,IL^(-1)βand CRP in the mouse serum and colorectal tumor in a dose-dependent *** staining showed that UA lessened carcinogenesis in the colorectum,with lower infiltration of lymphocytes,versus the *** indicated that UA mitigated the expression of Wnt4,β-catenin,TCF

关键词： ursolic acid colitis associated cancer Wnt/β-catenin signaling pathway

略论职工技协在实施“经济技术创新工程”中的地位和作用

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中国劳动关系学院学报 2001年第4期15卷 41-43页

作者：邱茂伦山东枣庄市总工会!山东枣庄277102

职工技协是工会领导下的科技性社会团体 ,是推动“经济技术创新工程”实施的生力军。工会只有将职工技协作用发挥好 ,才能有效地促进“经济技术创新工程”的实施。

关键词：职工技协创新工程地位作用

Effect of 211At treating pollen and stigma on generative cells and seed setting of rice

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Nuclear Science and Techniques 1998年第3期 45-47页

作者： Jin Jian Nan 1, Chen Fang 2, Mo Shang Wu 1, Liu Ning 1, Zhang Yi Zheng 2, Gao mao Guo 2, Zhou mao lun 1 and Zhang Shu Yuan 1 ( 1Institute of Nuclear Science and Technology, 2Department of Biology, Sichuan Union University,

１ＩｎｔｒｏｄｕｃｔｉｏｎＴｒａｄｉｔｉｏｎａｌγｒａｙｉｓｓｔｉｌｕｓｅｄａｓａｍａｉｎｍｕｔａｇｅｎｉｃｓｏｕｒｃｅｆｏｒｔｈｅｒａｄｉａｔｉｏｎｉｎｄｕｃｅｄｍｕｔａｔｉｏｎｒｅｓｅａｒｃｈｉｎｐｌａｎｔ... 详细信息

１ＩｎｔｒｏｄｕｃｔｉｏｎＴｒａｄｉｔｉｏｎａｌγｒａｙｉｓｓｔｉｌｕｓｅｄａｓａｍａｉｎｍｕｔａｇｅｎｉｃｓｏｕｒｃｅｆｏｒｔｈｅｒａｄｉａｔｉｏｎｉｎｄｕｃｅｄｍｕｔａｔｉｏｎｒｅｓｅａｒｃｈｉｎｐｌａｎｔｓ．Ｉｎｔｈｅｍｅａｎｔｉｍｅ，ｔ...

关键词： At Rice Generative cells Seed setting rate Induced variation

山东省肉羊养殖适度规模探究——基于山东省306户规模化养殖场(户)的调查数据

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湖北农业科学 2019年第13期58卷 91-96页

作者：张茂伦山东农业大学经济管理学院

基于实地问卷调查所获得的山东省17地(市)306户规模化养殖场(户)肉羊养殖成本收益的调查数据,运用SPSS22.0计量分析软件,基于盈亏平衡对山东省企业化养殖场(户)与规模化养殖农户的肉羊养殖适度规模分别进行了计算确定。结果表明,山东省... 详细信息

基于实地问卷调查所获得的山东省17地(市)306户规模化养殖场(户)肉羊养殖成本收益的调查数据,运用SPSS22.0计量分析软件,基于盈亏平衡对山东省企业化养殖场(户)与规模化养殖农户的肉羊养殖适度规模分别进行了计算确定。结果表明,山东省企业化肉羊养殖场(户)的肉羊养殖规模在529~2406只范围内才可保本,当养殖规模为1467只时可实现利润最大化;山东省规模化养殖农户的肉羊养殖规模在40~394只范围内才可保本,当养殖规模为217只时可实现利润最大化。

关键词：适度规模养殖盈亏平衡肉羊山东省