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Screening and identification of mimotopes of the major shrimp allergen tropomyosin using one-bead-one- compound peptide libraries

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Cellular & Molecular Immunology 2017年第3期14卷 308-318页

作者： Nicki YH Leung Christine YY Wai Marco HK Ho Ruiwu Liu Kit S lam Jin Jun Wang Shang An Shu ka Hou Chu Patrick SC Leung School of Life Sciences The Chinese University of Hong Kong Shatin N.T. Hong Kong SAR China Department of Pediatrics and Adolescent Medicine Queen Mary Hospital The University of Heng Kong Pokfulam Hong Kong SAR China Department of Biochemistry and Molecular Medicine University of California Davis CA 95616 USA Division of Rheumatology/Allergy School of Medicine University of California Davis CA 95616 USA

The one-bead-one-compound （OBOC） combinatorial peptide library is a powerful tool to identify ligand and receptor interactions. Here, we applied the OBOC library technology to identify mimotopes specific to the immunoglobulin E （IgE） epitopes of the major shellfish allergen tropomyosin. OBOC peptide libraries with 8-12 amino acid residues were screened with serum samples from patients with shellfish allergy for IgE mimotopes of tropomyosin. Twenty-five mimotopes were identified from the screening and their binding reactivity to tropomyosin-specific IgE was confirmed by peptide ELISA. These mimotopes could be divided into seven clusters based on sequence homology, and epitope mapping by EpiSearch of the clustered mimotopes was performed to characterize and confirm the validity of mimotopes. Five out of six of the predicted epitopes were found to overlap with previously identified epitopes of tropomyosin. To further confirm the mimicry potential of mimotopes, BALB/c mice were immunized with mimotopes conjugated to keyhole limpet hemocyanin and assayed for their capacity to induce tropomyosin-specific antibodies. BALB/c mice that received mimotope immunization were found to have an elevated level of tropomyosin-specific immunoglobulin G, but not mice that received an irrelevant mimotope. This study pioneers the successful application of the OBOC libraries using whole sera to screen and identify multiple shrimp allergen mimotopes and validates their mimicry potential using in vitro, in vivo, and in silico methods.

关键词： epitope mimotope OBOC peptide library tropomyosin

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A machine learning model for colorectal liver metastasis post-hepatectomy prognostications

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Hepatobiliary Surgery and Nutrition 2023年第4期12卷 495-506,I0007-I0016页

作者： Cynthia Sin Nga lam Alina Ashok Bharwani Evelyn Hui Yi Chan Vernice Hui Yan Chan Howard Lai Ho Au Margaret kay Ho Shireen Rashed Bernard Ming Hong Kwong Wentao Fang ka Wing Ma Chung Mau Lo Tan To Cheung Li ka Shing Faculty of Medicine The University of Hong KongHong Kong SARChina Department of Surgery Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina

Background:Currently,surgical resection is the mainstay for colorectal liver metastases(CRLM)management and the only potentially curative treatment *** tools can support patient selection for surgical resection to maximize therapeutic *** study aimed to develop a survival prediction model using machine learning based on a multicenter patient sample in Hong ***:Patients who underwent hepatectomy for CRLM between 1 January 2009 and 31 December 2018 in four hospitals in Hong Kong were included in the *** analysis was performed using Cox proportional hazards(CPH).A stepwise selection on Cox multivariable models with Least Absolute Shrinkage and Selection Operator(LASSO)regression was applied to a multiply-imputed dataset to build a prediction *** model was validated in the validation set,and its performance was compared with that of Fong Clinical Risk Score(CRS)using concordance ***:A total of 572 patients were included with a median follow-up of 3.6 *** full models for overall survival(OS)and recurrence-free survival(RFS)consist of the same 8 established and novel variables,namely colorectal cancer nodal stage,CRLM neoadjuvant treatment,Charlson Comorbidity Score,pre-hepatectomy bilirubin and carcinoembryonic antigen(CEA)levels,CRLM largest tumor diameter,extrahepatic metastasis detected on positron emission-tomography(PET)-scan as well as KRAS *** CRLM Machine-learning Algorithm Prognostication model(CMAP)demonstrated better ability to predict OS(C-index=0.651),compared with the Fong CRS for 1-year(C-index=0.571)and 5-year OS(C-index=0.574).It also achieved a C-index of 0.651 for ***:We present a promising machine learning algorithm to individualize prognostications for patients following resection of CRLM with good discriminative ability.

关键词： Machine-learning colorectal liver metastasis(CRLM) prognostic model survival analysis hepatectomy outcome

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Early-onset primary torsional dystonia in a 4-generation Chinese family with a mutation in the DYT1 gene

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Chinese Medical Journal 2005年第10期118卷 873-876页

作者： YEUNG Wai Lan lam Ching Wan CHENG Wai Tsoi SIN Ngai Chuen WONG Wing Kin WONG Chun Nei TSE ka Ming FOK Tai Fai Departments of Paediatrics Prince of Wales HospitalThe Chinese University of Hong KongHong KongChina Chemical Patholog Prince of Wales HospitalThe Chinese University of Hong KongHong KongChina Department of Paediatrics and Adolescent Medicine Queen Mary HospitalUniversity of Hong Kong Nuclear Medicine&PET Division Scanning DepartmentSt.Teresa’s HospitalHong KongChina

Primary torsion dystonia(PTD)is a clinically and genetically heterogeneous movement *** least thirteen different types of dystonia can be distinguished on a genetic basis.~1 The DYT1 gene was first mapped by Ozelius et al in 1989.~2(Kramer et)al^3 linked the same locus to PTD in 12 Ashkenazi Jewish families in *** patients with early-onset generalized PTD were caused by the same three base pair(GAG)deletion in the DYT1 gene on chromosome 9q34.^(1,4,5)The product of the gene is a protein called torsinA.~5(Although the)function of this protein is as yet uncertain,it is widely distributed throughout the brain with high levels in the substantia nigra compacta dopamine neurones.

关键词： DYT1 gene dystonia positron emission tomography

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HM30181A,a potent P-glycoprotein inhibitor,potentiates the absorption and in vivo antitumor efficacy of paclitaxel in an orthotopic brain tumor model

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Cancer Biology & Medicine 2020年第4期17卷 986-1001页

作者： Wu Zeng Betty Yuen Kwan Law Vincent kam Wai Wong Denise So Bik Chan Simon Wing Fai Mok Joyce Jia Ying Gao Rebecca ka Yan Ho Xu Liang Jia Hao Li Ming Tsung Lee Weng Li Yoon Michael P Smolinski Johnson Yiu Nam Lau Christopher Wai Kei lam Manson Fok State Key Laboratory of Quality Research in Chinese Medicines Macao University of Science and TechnologyMacaoChina At he nex Hong Kong Irmo vative Limited Hong Kong China Faculty of Medici ne Macao Un iversity of Science and TechnologyMacaoChina Athenex Inc. New York 14203USA

Objective:Delivery of chemotherapeutic drugs to the brain has remained a major obstacle in the treatment of glioma,owing to the presence of the blood-brain barrier and the activity of P-gp,which pumps its substrate back into the systemic *** aim of the present study was to develop an intravenous formulation of HM30181 A(HM)to inhibit P-gp in the brain to effectively deliver paclitaxel(PTX)for the treatment of malignant ***:Two formulations of solubilized HM were designed on the basis of different solid dispersion strategies:i)spray-drying[polyvinlypyrrolidone(PVP)-HM]and ii)solvent evaporation[HP-β-cyclodextrin(cyclodextrin)-HM].The P-gp inhibition of these 2 formulations was assessed on the basis of rhodamine 123 uptake in cancer *** and brain pharmacokinetic parameters were also determined,and the antitumor effect of cyclodextrin-HM with PTX was evaluated in an orthotopic glioma xenograft mouse ***:Although both PVP-HM and cyclodextrin-HM formulations showed promising P-gp inhibition activity in vitro,cyclodextrin-HM had a higher maximum tolerated dose in mice than did *** study of cyclodextrin-HM revealed a plasma concentration plateau at 20 mg/kg,and the mice began to lose weight at doses above this ***-HM(10 mg/kg)administered with PTX at 10 mg/kg showed optimal antitumor activity in a mouse model,according to both tumor volume measurement and survival time(P<0.05).Conclusions:In a mouse orthotopic brain tumor model,the intravenous co-administration of cyclodextrin-HM with PTX showed potent antitumor effects and therefore may have potential for glioma therapy in humans.

关键词： HM30181A glioma pharmacokinetics paclitaxel P-glycoprotein

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A novel design of Vertical Patch Antenna with corrugated ground structure

A novel design of Vertical Patch Antenna with corrugated gro...

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2005'全国微波毫米波会议

作者： ka-lam Lau T.P.Wong K.M.Luk K.F.Lee

＜正＞This paper presents a novel design for a circular vertical patch antenna （VPA）.The circular-VPA is surrounded by a circular corrugated ground and it has around 10%impedance bandwidth and 8dBi *** design yields a significant reduction in cross-polarization radiation and back radiation levels over a bandwidth in excess of 9.5%.A 15dB reduction in the cross polarization and 15dB suppression in the backlobe were obtained at the center *** and broadside patterns are maintained across the matching *** of the proposed antenna,simulation and experimental results are presented and discussed.

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Wideband Vertical Patch Antenna

Wideband Vertical Patch Antenna

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2005'全国微波毫米波会议

作者： T.P.Wong ka-lam Lau K.M.Luk K.F.Lee

＜正＞Vertical patch antennas（VPAs） based on Koch loop fractal structure[1-6]are *** between Koch fractal VPAs in different iterations and a circular VPA with same size and same height are *** a dual Koch loop structure,a wideband VPA with 42%bandwidth and 8dBi gain at the center frequency is designed and *** broadside patterns are obtained at the passband.

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Microbial community coexisting with harmful alga karenia mikimotoi and microbial control of algal bloom in laboratory

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Journal of Oceanology and Limnology 2022年第3期40卷 1027-1038页

作者： Li SUN Peike GAO Yu LI Chao WANG Ning DING Junfeng CHEN Yuhao SONG Chunchen LIU lun SONG Renjun WANG College of Life Sciences Qufu Normal UniversityQufu 273165China Key Laboratory of Marine Biological Resources and Ecology Liaoning Ocean and Fisheries Science Research InstituteDalian 116023China

Algicidal bacteria have been frequently isolated from algal blooming ***,knowledge regarding the microbial communities coexisting with microalgae and their potential application in preventing harmful algal blooms(HABs)is *** this study,we investigated the composition of the microbial community coexisting with harmful alga karenia mikimotoi and its responses to algal control via nutrient stimulation or by adding algicidal strain in *** microorganisms inhabiting the *** culture consisted of 24 identifi ed phyla,including dominant Proteobacteria(relative abundance 76.24%±7.28%)and Bacteroidetes(22.67%±8.32%).Rhodobacteraceae,Phaeodactylibacter,and Maritimibacter predominated during the algal *** the added nutrient and fermentation broth of algicidal strain Pseudoalteromonas QF1 caused a massive death of *** and substantial changes in the coexisting microbial community,in which Rhodobacteraceae and Phaeodactylibacter signifi cantly decreased,while Halomonas and Alteromonas *** operational taxonomic units(OTUs)analysis indicated that 13 OTUs belonging to Rhodobacteraceae,Maritimibacter,Marivita,Nisaea,Phaeodactylibacter,Citreicella,Halomonas,Alteromonas,Marinobacter,Muricauda,and Pseudoalteromonas dominated the changes of the microbial communities observed in the *** culture with or without ***,this study indicated that microbial community inhabiting *** culture includes potential algicidal bacteria,and improves our knowledge about microbial community succession during biocontrol of *** via nutrient stimulation or by adding isolated algicidal strains.

关键词： karenia mikimotoi microbial community nutrient stimulation algicidal bacteria Pseudoalteromonas

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A WiMAX-ZigBee Energy Management System for Green Education

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Smart Grid and Renewable Energy 2011年第4期2卷 338-348页

作者： Hoi Yan Tung Kim Fung Tsang ka Lum lam Hoi Ching Tung Rong Jian Zheng King Tim Ko Loi Lei Lai 不详

A WiMAX-ZigBee energy management system for green education has been developed. The system helps to resolve the disgusting environmental problems. This paper explores the concept of energy management and introduces an Energy Management System located at campus areas. Analysis shows that 30% energy can be saved from the total energy consumption which translates to a saving of 72 thousand million kWh of energy annually. Such saving reduces 48 million tons of CO2 emission for the environment.

关键词： Energy Management Minimizing CO2 Generation WiMAX ZigBee Green Education

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A reference-grade genome of the xerophyte Ammopiptanthus mongolicus sheds light on its evolution history in legumes and droughttolerance mechanisms

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Plant Communications 2024年第7期5卷 157-177页

作者： Lei Feng Fei Teng Na Li Jia-Cheng Zhang Bian-Jiang Zhang Sau-Na Tsai Xiu-Le Yue Li-Fei Gu Guang-Hua Meng Tian-Quan Deng Suk-Wah Tong Chun-Ming Wang Yan Li Wei Shi Yong-lun Zeng Yue-Ming Jiang Weichang Yu Sai-Ming Ngai Li-Zhe An Hon-Ming lam Jun-Xian He School of Life Sciences and State Key Laboratory of Agrobiotechnology The Chinese University of Hong KongSha TinNew TerritoriesHong KongChina Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life SciencesSouth China Normal UniversityGuangzhou 510631China BGI-Shenzhen Tech Co. Ltd.Beishan Industrial ZoneYantian DistrictShenzhen 518083China School of Life Sciences and Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations Lanzhou UniversityLanzhou 730030China State Key Laboratory of Subtropical Silviculture Zhejiang Agriculture and Forestry UniversityHangzhou 311300China State Key Laboratory of Plant Diversity and Specialty Crops South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China Guangdong Provincial Key Laboratory of Applied Botany&Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement South China Botanical GardenChinese Academy of SciencesGuangzhou 510650China College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518060China State Key Laboratory of Efficient Production of Forest Resources Beijing Forestry UniversityBeijing 100083China

Plants that grow in extreme environments represent unique sources of stress-resistance genes and *** mongolicus(Leguminosae)is a xerophytic evergreen broadleaf shrub native to semi-arid and desert regions;however,its drought-tolerance mechanisms remain poorly ***,we report the assembly of a reference-grade genome for ***,describe its evolutionary history within the legume family,and examine its drought-tolerance *** assembled genome is 843.07 Mb in length,with 98.7%of the sequences successfully anchored to the nine chromosomes of *** genome is predicted to contain 47611 protein-coding genes,and 70.71%of the genome is composed of repetitive sequences;these are dominated by transposable elements,particularly longterminal-repeat *** analyses revealed two whole-genome duplication(WGD)events at 130 and 58 million years ago(mya)that are shared by the genus Ammopiptanthus and other legumes,but no species-specific WGDs were found within this *** genome reconstruction revealed that the *** genome has undergone fewer rearrangements than other genomes in the legume family,confirming its status as a"relict plant".Transcriptomic analyses demonstrated that genes involved in cuticular wax biosynthesis and transport are highly expressed,both under normal conditions and in response to polyethylene glycol-induced *** induction of genes related to ethylene biosynthesis and signaling was also observed in leaves under dehydration stress,suggesting that enhanced ethylene response and formation of thick waxy cuticles are two major mechanisms of drought tolerance in *** expression of AmERF2,an ethylene response factor unique to ***,can markedly increase the drought tolerance of transgenic Arabidopsis thaliana plants,demonstrating the potential for application of *** genes in crop improvement.

关键词： Ammopiptanthus mongolicus genome sequencing genome evolution drought tolerance cuticular wax ethylene

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中国吸毒与艾滋病状况调查的综述和评论

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社会工作 2010年第2期 4-9页

作者：林卡周伦浙江大学公共管理学院浙江杭州310000 南京大学社会学院江苏南京210000

社会发展进程势必伴随着新的许多社会问题的产生和解决。自20世纪80年代以来,吸毒和艾滋病的传播逐渐成为危及公共健康、社会治安和社会控制的焦点问题。针对这些问题,国内学者和政府机构展开了一系列的调查研究工作,以便为缓解这些社... 详细信息

社会发展进程势必伴随着新的许多社会问题的产生和解决。自20世纪80年代以来,吸毒和艾滋病的传播逐渐成为危及公共健康、社会治安和社会控制的焦点问题。针对这些问题,国内学者和政府机构展开了一系列的调查研究工作,以便为缓解这些社会问题,增进社会治理的成效寻找有效途径。本文将回顾这些研究,并由此反映这一研究领域中所取得的最新进展。文章把研究的落脚点放在与吸毒人群的艾滋病传播风险相关问题的文献评述上,以期为展开艾滋病防范和对吸毒人群的矫治等方面的工作提供一定的理论帮助。

关键词：社会问题吸毒艾滋病行为矫治社会工作

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