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Activities of some defense proteins associated with age or plant family in larch needles of Larix olgensis and L. kaempferi× L. gmelinii

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Journal of Forestry Research 2017年第1期28卷 63-69页

作者： Zhaojun Meng Lili Tong lulu gao Shanchun Yan Yifang Lu College of Forestry Northeast Forestry University

Defence proteins are a kind of chemical defence compounds. They play a key role in plant restraining biotic and abiotic harm. To illuminate activities of some defense proteins associated with age or plant family of larch, the larch needles were collected from two different families of Korean larch, Larix olgensis and a hybrid larch, L. kaempferi × L. gmelinii, respectively, and then the activities of defence proteins in those were tested using a UV spectrophotometry. The results showed that the activities of protective enzymes at thel6-/17- and 19-year-age groups were higher than those at the other age groups in the both larch species. While the activities of polyphenol oxidase （PPO） at the 16-/17-year-age group and phenylalanine ammonia-lyase （PAL） and protease inhibitors at the 19-year-age group were the highest among all age groups. Then, compared with L. olgensis, the hybrid larch had significant effects on the activities of protective enzymes. The effects of plant family on the activities of PAL and chymotrypsin inhibitor were significantly different, and then those on the activities of PPO were not significantly different. The effects of the two families in L. olgensis on the activities of trypsin inhibitor （TI） were significantly different, while those in the hybrid larch on the activities of TI were contrary. To conclusions showed that the different age and plant family significantly affected the activities of defence proteins in the needles of two larch species, and then enhanced the larch resistance to pests. These could play a key function in forestry tree genetic improvement and management in future.

关键词： Tree age Chemical defence Defenceprotein Larch Plant family

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Spectroscopy-Based Soil Organic Matter Estimation in Brown Forest Soil Areas of the Shandong Peninsula, China

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Pedosphere 2019年第6期29卷 810-818页

作者： gao lulu ZHU Xicun HAN Zhaoying WANG Ling ZHAO Gengxing JIANG Yuanmao College of Resource and Environment Shandong Agricultural UniversityTai’an 271018(China National Engineering Laboratory for Efficient Utilization of Soil Resources Tai’an 271018(China College of Horticulture Science and Engineering Shandong Agricultural UniversityTai’an 271018(China

Soil organic matter (SOM) is important for plant growth and production. Conventional analyses of SOM are expensive and time consuming. Hyperspectral remote sensing is an alternative approach for SOM estimation. In this study, the diffuse reflectance spectra of soil samples from Qixia City, the Shandong Peninsula, China, were measured with an ASD FieldSpec 3 portable object spectrometer (Analytical Spectral Devices Inc., Boulder, USA). Raw spectral reflectance data were transformed using four methods: nine points weighted moving average (NWMA), NWMA with first derivative (NWMA + FD), NWMA with standard normal variate (NWMA + SNV), and NWMA with min-max standardization (NWMA + MS). These data were analyzed and correlated with SOM content. The evaluation model was established using support vector machine regression (SVM) with sensitive wavelengths. The results showed that NWMA + FD was the best of the four pretreatment methods. The sensitive wavelengths based on NWMA + FD were 917, 991, 1 007, 1 996, and 2 267 nm. The SVM model established with the above-mentioned five sensitive wavelengths was significant ( R 2 = 0.875, root mean square error (RMSE) = 0.107 g kg −1 for calibration set;R 2 = 0.853, RMSE = 0.097 g kg −1 for validation set). The results indicate that hyperspectral remote sensing can quickly and accurately predict SOM content in the brown forest soil areas of the Shandong Peninsula. This is a novel approach for rapid monitoring and accurate diagnosis of brown forest soil nutrients.

关键词： Brown forest soil Hyperspectral remote sensing Nine points weighted moving average Standard normal variate Sensitive wavelength Spectral reflectance Support vector machine regression

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Inhibiting oxygen vacancies and twisting NbO_(6) octahedron in erbium modified KNN-based multifunctional ceramics

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Journal of Materiomics 2024年第1期10卷 179-189页

作者： lulu gao Zhiyong Liu Pengrong Ren Renhong Liang Ting Li Kun Guo Bing Xie Jinshan Lu Pu Mao Jun Tian Longlong Shu School of Materials Science and Engineering Nanchang Hangkong UniversityNanchang330063China School of Materials Science and Engineering Xi'an University of TechnologyXi'an710048China School of Physics and Materials Science Nanchang UniversityNanchang330031China Institute of Applied Chemistry Jiangxi Academy of SciencesNanchang330096China

It is a challenge to obtain highly tunable multifunctional performances in one ferroelectric system by a simple approach to meet the miniaturization,integration,and functionalization requirements of advanced electronic ***,rare earth erbium(Er)modulated 0.9K_(0.5)Na_(0.5)NbO_(3)-0.1Sr(1-x)Er_(x)Ti_((1-x/4))O_(3),(0.9KNN-0.1ST:xEr)transparent-photoluminescent-ferroelectric energy storage multifunctional ceramics are prepared to solve this *** effect of lattice distortion and oxygen vacancies by Er doping on the optical and electrical properties is systematically *** Er^(3+)ions can introduce a large distortion of the NbO_(6) octahedron by replacing the A-site in KNN-based *** to the higher c/a ratio and lower oxygen vacancy content are simultaneously obtained in 0.9KNN-0.1ST:0.1Er *** effective energy storage density(Wrec)of 0.86 J/cm^(3),excellent near-infrared transmittance of 51.7%(1100 nm)and strong green upconversion photoluminescence are achieved in this multifunctional *** study provides a solid basis for rare earth ions doped ferroelectric ceramics with tunable multifunctional properties and has significant potential for applications in optoelectronic devices.

关键词： KNN Upconversion photoluminescence Multifunctional ceramics Oxygen vacancy Octahedron

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Positional cloning and characterization reveal the role of a miRNA precursor gene ZmLRT in the regulation of lateral root number and drought tolerance in maize

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Journal of Integrative Plant Biology 2023年第3期65卷 772-790页

作者： Ming Zhang Yanhong Chen Hongyan Xing Wensheng Ke Yunlu Shi Zhipeng Sui Ruibin Xu lulu gao Ganggang Guo Jiansheng Li Jiewen Xing Yirong Zhang State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis and Utilization(MOE)Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Agronomy College of Shandong Agricultural University Taian 271018China Key L aboratory of Crop Germplasm Resources and Utilization(MARA) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Center for Crop Functional Genomics and Molecular Breeding China Agricultural UniversityBeijing 100193China Yantai Science and Technology Innovation Promotion Center Yantai 264003China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Maize Research InstituteBeijing Academy of Agriculture and Forestry SciencesBeijing 100097China National Maize Improvement Center of China China Agricultural UniversityBeijing 100193China

Lateral roots play essential roles in drought tolerance in maize(Zea mays L.). However, the genetic basis for the variation in the number of lateral roots in maize remains elusive. Here, we identified a major quantitative trait locus(QTL),q LRT5-1, controlling lateral root number using a recombinant inbred population from a cross between the maize lines Zong3(with many lateral roots) and 87-1(with few lateral roots).Fine-mapping and functional analysis determined that the candidate gene for qLRT5-1,ZmLRT, expresses the primary transcript for the microRNA miR166a. ZmLRT was highly expressed in root tips and lateral root primordia,and knockout and overexpression of ZmLRT increased and decreased lateral root number,respectively. Compared with 87-1, the ZmLRT gene model of Zong3 lacked the second and third exons and contained a 14 bp deletion at the junction between the first exon and intron,which altered the splicing site. In addition,ZmLRT expression was significantly lower in Zong3 than in 87-1, which might be attributed to the insertions of a transposon and over large DNA fragments in the Zong3 ZmLRT promoter region. These mutations decreased the abundance of mature miR166a in Zong3, resulting in increased lateral roots at the seedling ***, miR166a post-transcriptionally repressed five development-related class-Ⅲ homeodomain-leucine zipper genes. Moreover,knockout of ZmLRT enhanced drought tolerance of maize seedlings. Our study furthers our understanding of the genetic basis of lateral root number variation in maize and highlights ZmLRT as a target for improving drought tolerance in maize.

关键词： drought tolerance lateral root number maize miRNA precursor QTL mapping

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Transcriptome analysis of Clematis lanuginosa: Novel features of the molecular events occurring under heat-shock stress

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Ornamental Plant Research 2022年第1期2卷 184-192页

作者： Linfang Li Shuan Wang Qiao Li Linghe Sun Xin Han lulu gao Peng Wang Fenni Lv Rutong Yang Ya Li Institute of Botany Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen)Nanjing 210014China

The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the *** elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of the Clematis large-flowered group of ornamental varieties,was selected for use in this ***,six libraries,including three biological replicates each of control and heat-shock stress samples,were determined using RNA-sequencing *** total,62,050 unigenes were obtained,and 6,439 unigenes exceeded 1 kb in length.A total of 42,377 unigenes were annotated using six *** the two treatments,2,165 differentially expressed genes were identified,with 1,565 being up-regulated and 600 *** addition,51 heat-shock protein-encoding genes were identified,among which the small heat-shock proteins accounted for 68.63%.In total,two heat-shock factors and 12 ribosomal proteins were significantly up-regulated under heatstress *** differential expressions of ethylene-responsive transcription factor,chalcone synthase,cysteine-rich receptor-like kinase and aspartic protease unigenes in guard cells were induced by *** data obtained will assist the elucidation of the molecular events underlying heat-stress responses in ***.

关键词： shock stress ipt

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Estimating Canopy Nitrogen Contents of an Apple Tree Using Hyperspectral Remote Sensing

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遥感科学（中英文版） 2016年第2期4卷 42-50页

作者： Xicun ZHU lulu gao Xianyi FANG Gengxing ZHAO Ling WANG College of Resources and Environment Shandong Agricultural UniversityTaianShandong271018China

Estimating an apple tree canopy nitrogen contents using hyperspectral techniques is important in theoretical and realistic significance for fertilization and *** 80 Fuji apple trees at the early fruiting stage as the research objects,the hyperspectral characteristics of the apple canopy were analyzed *** apple canopy hyperspectral and the canopy nitrogen contents were measured *** canopy hyperspectral characteristics under different nitrogen contents were analyzed and selected the sensitive wave *** apple canopy nitrogen content monitoring models were established by using multiple regression method,robust regression and BP neural network *** results showed that the canopy hyperspectral reflectance had obvious differences under different nitrogen *** sensitive bands concentrate on 724~1136 *** models based on hyperspectral indices are not *** based on robust regression(M regression)and BP neural network are better than multiple statistical model,and the accuracy of the BP neural network monitoring model is the *** results of the study provide a certain reference for estimating apple nutrition using hyperspectral technology.

关键词： Apple Ttrees Canopy Nitrogen Contents Hyperspectrum Estimating Model

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Paternally imprinted LATE-FLOWERING2 transcription factor contributes to paternal-excess interploidy hybridization barriers in wheat∞

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Journal of Integrative Plant Biology 2023年第12期65卷 2587-2603页

作者： Guanghui Yang Man Feng Kuohai Yu Guangxian Cui Yan Zhou Lv Sun lulu gao Yumei Zhang Huiru Peng Yingyin Yao Zhaorong Hu Vincenzo Rossi Ive De Smet Zhongfu Ni Qixin Sun Mingming Xin State Key Laboratory for Agrobiotechnology Key Laboratory of Crop Heterosis Utilization(MOE)Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Qingdao Agricultural University Qingdao 266109China Council for Agricultural Research and Economics Research Centre for Cereal and Industrial CropsBergamo 24126Italy Department of Plant Biotechnology and Bioinformatics Ghent UniversityGhent B‐9052Belgium VIB Center for Plant Systems Biology Ghent B‐9052Belgium

Interploidy hybridization between hexaploid and tetraploid genotypes occurred repeatedly during genomic introgression events throughout wheat evolution,and is commonly employed in wheat breeding *** wheat usually serves as maternal parent because the reciprocal cross generates progeny with severe defects and poor seed germination,but the underlying mechanism is poorly ***,we performed detailed analysis of phenotypic variation in endosperm between two interploidy reciprocal crosses arising from tetraploid(Triticum durum,AABB)and hexaploid wheat(Triticum aestivum,AABBDD).In the paternal‐versus the maternal‐excess cross,the timing of endosperm cellularization was delayed and starch granule accumulation in the endosperm was repressed,causing reduced germination *** expression profiles of genes involved in nutrient metabolism differed strongly between these endosperm ***,expression patterns of parental alleles were dramatically disturbed in interploidy versus intraploidy crosses,leading to increased number of imprinted *** endosperm‐specific TaLFL2 showed a paternally imprinted expression pattern in interploidy crosses partially due to allele‐specific DNA *** TaLFL2 binds to and represses a nutrient accumulation regulator TaNAC019,leading to reduced storage protein and starch accumulation during endosperm development in paternal‐excess cross,as confirmed by interploidy crosses between tetraploid wild‐type and clustered regularly interspaced palindromic repeats(CRISPR)–CRISPR‐associated protein 9 generated hexaploid *** findings reveal a contribution of genomic imprinting to paternal‐excess interploidy hybridization barriers during wheat evolution history and explains why experienced breeders preferentially exploit maternal‐excess interploidy crosses in wheat breeding programs.

关键词： imprinting interploidy cross wheat

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Stereological and Pathological Study on the Ultrastructure of Vero Cells Infected Enterovirus 71

Stereological and Pathological Study on the Ultrastructure o...

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第十四届中国体视学与图像分析学术会议

作者： Shen Hong Lin Peixin gao lulu Huang Yeen Zhang Yaozhong Chen Qing Dept.of Pathology Southern Medical University Dept.of Epidemiology Southern Medical University

Introduction Enterovirus 71(EV71)can cause hand,foot and mouth *** study aim to find the stereological characteristics of vero cells infected EV71 in uhrastructure,to explore its pathogenesis *** and Methods Vero cells infected EV71 were observedwith transmission electron *** were cut in *** magnification times is one hundred thousand for

关键词： Enterovirus 71(EV71) Vero cells Mitochondria Stereology Pathology

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Improve the Prediction Accuracy of Apple Tree Canopy Nitrogen Content through Multiple Scattering Correction Using Spectroscopy

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Agricultural Sciences 2016年第10期7卷 651-659页

作者： lulu gao Xicun Zhu Cheng Li Lizhen Cheng Ling Wang Gengxing Zhao Yuanmao Jiang College of Resources and Environment Shandong Agricultural University Taian Shandong National Engineering Laboratory on Efficient Utilization of Soil and Fertilization Taian Shandong College of Horticulture Science and Engineering Shandong Agricultural University Taian Shandong

Method: Use Multiple Scattering Correction to eliminate the interference of scattering on spectrum in the process of field measurement so as to improve the accuracy of prediction model of tree canopy nitrogen content. Apple trees in Qixia of Yantai City were taken as the test material. The spectral reflectivity of apple tree canopy went through the First Derivative (FD) and Multiple Scattering Correction (MSC) plus first derivative, respectively. The correlation coefficients were calculated between spectral reflectivity and nitrogen content. The Support Vector Machine (SVM) method was used to establish the prediction model. The result indicates that the MSC pre-processing can improve the correlation between spectral reflectivity and nitrogen content. The SVM model with MSC + FD pre-processing was a good way to predict the nitrogen content. The calibration R2 of the model was 0.746;the validation R2 was 0.720;and its RMSE was 0.452 g·kgˉ1. MSC can commendably eliminate scattering error to improve the prediction accuracy of prediction model.

关键词： Multiple Scattering Correction Hyperspectrum Apple Tree Canopy Nitrogen Content Support Vector Machine

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批量制备静电纺有序纳米纤维的研究进展

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现代纺织技术 2021年第2期29卷 7-21页

作者：高璐璐徐岚苏州大学纺织与服装工程学院江苏苏州215123 苏州大学现代丝绸国家工程实验室江苏苏州215123

静电纺丝是一种常用且有效地制备纳米纤维的方法,但其制备的纳米纤维多为无序排列结构,具有各向同性、力学性能较差等缺陷,限制了纳米纤维的应用。因此,对静电纺丝装置进行相应的改进,从而得到具有各向异性且力学性能较好的有序纳米纤维... 详细信息

静电纺丝是一种常用且有效地制备纳米纤维的方法,但其制备的纳米纤维多为无序排列结构,具有各向同性、力学性能较差等缺陷,限制了纳米纤维的应用。因此,对静电纺丝装置进行相应的改进,从而得到具有各向异性且力学性能较好的有序纳米纤维,可进一步拓宽纳米纤维的应用领域。此外,传统单针头静电纺丝技术制备效率低,使得静电纺有序纳米纤维产量不高。为了提升产量,研究人员提出了多针头及无针头静电纺丝技术。本文从静电纺有序纳米纤维和批量制备静电纺纳米纤维两个角度出发,重点分析了为提高纳米纤维有序度和生产效率而使用的各种静电纺丝设备,系统概述了批量制备静电纺有序纳米纤维的研究进展。

关键词：静电纺丝装置批量制备有序纳米纤维

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