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Effect of ions on the activity of brain acetylcholinesterase from tropical ifsh

Journal of Coastal Life Medicine 2015年第7期 505-514页

作者： Caio rodrigo Dias Assis Amanda Guedes Linhares Vagne Melo Oliveira renata Cristina Penha Franca Juliana ferreira Santos Elba Verônica Matoso Maciel Carvalho ranilson Souza Bezerra luiz Bezerra Carvalho Jr Laboratory of Enzymology-LABENZ Biochemistry Department and Laboratory of Immunopathology Keizo AsamiFederal University of PernambucoRecife-PEBrazil Academic Unit of Serra Talhada Federal Rural University of PernambucoSerra Talhada-PEBrazil Laboratory of Glycoproteins Biochemistry DepartmentFederal University of PernambucoRecife-PEBrazil

Objective:To investigate the effect of ions on brain acetylcholinesterase(AChE;EC 3.***.1.7)activities from economic important fish[pirarucu,Arapaima gigas;tambaqui,Colossoma macropomum;cobia,rachycentron canadum(***)and Nile tilapia,Oreochromis niloticus(***)]comparing with a commercial enzyme from electric eel[Electrophorus electricus(***)].Methods:Thein vitro exposure was performed at concentrations ranging from 0.001 to 10 mmol/L(except for ethylene diamine tetraacetic acid;up to 150 mmol/L).Inhibition kinetics *** *** were also observed through four methods(Michaelis-Menten,Lineweaver-Burk,Dixon and Cornish-Bowden plots)in order to investigate the type of inhibition produced by some ***:Hg^(2+),As^(3+),Cu^(2+),Zn^(2+),Cd^(2+)caused inhibition in all the species under ***^(2+),Mg^(2+)and Mn^(2+)induced slight activation *** enzyme while Pb^(2+),Ba^(2+),Fe^(2+),Li+inhibited the AChE from some of the analyzed *** lowest IC50 and Ki values were estimated *** AChE in presence of Hg^(2+),Pb^(2+),Zn^(2+).Under our experimental conditions,the results *** ***,As^(3+),Cu^(2+),Cd^(2+),Pb^(2+)and Zn^(2+)showed a non-competitive/mixed-type inhibition,while Hg^(2+)inhibited the enzyme in a mixed/competitive-like ***:*** AChE activity was affected by ten of fifteen ions under study showing that this enzyme could undergo interference by these ions when used as pesticide biosensor in environmental *** hindrance would be less relevant for the crude extracts.

关键词： Ions Acetylcholinesterase Biomarkers Fish

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