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Long-noncoding RNAs as epigenetic regulators in neurodegenerative diseases

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Neural Regeneration Research 2023年第6期18卷 1243-1248页

作者： Paola Ruffo Francesca De Amicis Emiliano Giardina Francesca luisa Conforti Medical Genetics Laboratory Department of PharmacyHealth and Nutritional SciencesUniversity of CalabriaRendeItaly Department of Pharmacy Health and Nutritional SciencesUniversity of CalabriaRendeItaly Genomic Medicine Laboratory UILDM IRCCS Fondazione Santa LuciaRomeItaly Department of Biomedicine&Prevention Tor Vergata University of RomeRomeItaly

The growing and rapid development of high-throughput sequencing technologies have allowed a greater understanding of the mechanisms underlying gene expression *** the epigenome and epitranscriptome directs the fate of the transcript influencing the functional outcome of each *** this context,non-coding RNAs play a decisive role in addressing the expression regulation at the gene and chromosomal ***-noncoding RNAs,consisting of more than 200 nucleotides,have been shown to act as epigenetic regulators in several key molecular processes involving neurodegenerative disorders,such as Alzheimer’s disease,Parkinson’s disease,amyotrophic lateral sclerosis and Huntington’s ***-noncoding RNAs are abundantly expressed in the central nervous system,suggesting that their deregulation could trigger neuronal degeneration through RNA *** evaluation of their diagnostic significance and therapeutic potential could lead to new treatments for these diseases for which there is no cure.

关键词： Alzheimer’s disease amyotrophic lateral sclerosis epigenetic mechanism Huntington’s disease long-noncoding RNAs neurodegenerative disease non-coding RNAs Parkinson’s disease

Diagnostic challenges in alcohol use disorder and alcoholic liver disease

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World Journal of Gastroenterology 2014年第25期20卷 8024-8032页

作者： luisa Vonghia Peter Michielsen Geert Dom Sven Francque Department of Gastroenterology and Hepatology University Hospital Antwerp Wilrijkstraat 10 2650 Edegem Belgium Department of Basic Medical Sciences Neuroscience and Sensory OrgansUniversity of BariPoliclinicoPiazza Giulio Cesare70100 BariItaly Collaborative Antwerp Psychiatric Research Institute (CAPRI) Antwerp University Universiteitsplein 12610 Antwerp Belgium

Alcohol use disorders represent a heterogeneous spectrum of clinical manifestations that have been defined by the Diagnostic and Statistical Manual of Mental Disorders-5. Excessive alcohol intake can lead to damage of various organs, including the liver. Alcoholic liver disease includes different injuries ranging from steatosis to cirrhosis and implicates a diagnostic assessment of the liver disease and of its possible complications. There is growing interest in the possible different tools for assessing previous alcohol consumption and for establishing the severity of liver injury, especially by noninvasive methods.

关键词： Alcoholic liver disease Alcohol use disorder Diagnosis Diagnostic and Statistical Manual of Mental Disorders-5 Screening tests Markers of previous alcohol consumption Non-invasive fibrosis assessment

Oleanolic acid:a promising neuroprotective agent for cerebral ischemia

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Neural Regeneration Research 2015年第4期10卷 540-541页

作者： Caltana Laura Nieto María luisa Brusco Alicia Instituto de Biología Celulary Neurociencia IBCN(UBA-CONICET)Buenos AiresArgentina Instituto Biologíay Genética Molecular CSIC-UVAValladolidSpain

Stroke is considered the most common and severely disabling neurological disease. It is one of the leading causes of death after heart disease and cancer, causing 10% deaths worldwide and involving risk factors such as smoking, obesity and nutritional disbalance. Disability affects 75% of stroke survivors through severe mental and/or physical impairment depending on the affected brain area （Go et al., 2014）.

关键词： acid Oleanolic acid

Mortality associated with hepatitis C and hepatitis B virus infection: A nationwide study on multiple causes of death data

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World Journal of Gastroenterology 2017年第10期23卷 1866-1871页

作者： Ugo Fedeli Enrico Grande Francesco Grippo luisa Frova Epidemiological Department Italian National Institute of Statistics

To analyze mortality associated with hepatitis C virus (HCV) and hepatitis B virus (HBV) infection in *** certificates mentioning either HBV or HCV infection were retrieved from the Italian National Cause of Death Register for the years 2011-2013. Mortality rates and proportional mortality (percentage of deaths with mention of HCV/HBV among all registered deaths) were computed by gender and age class. The geographical variability in HCV-related mortality rates was investigated by directly age-standardized rates (European standard population). Proportional mortality for HCV and HBV among subjects aged 20-59 years was assessed in the native population and in different immigrant *** infection was mentioned in 1.6% (n = 27730) and HBV infection in 0.2% (n = 3838) of all deaths among subjects aged ≥ 20 years. Mortality rates associated with HCV infection increased exponentially with age in both genders, with a male to female ratio close to unity among the elderly; a further peak was observed in the 50-54 year age group especially among male subjects. HCV-related mortality rates were higher in Southern Italy among elderly people (45/100000 in subjects aged 60-79 and 125/100000 in subjects aged ≥ 80 years), and in North-Western Italy among middle-aged subjects (9/100000 in the 40-59 year age group). Proportional mortality was higher among Italian citizens and North African immigrants for HCV, and among Sub-Saharan African and Asian immigrants for *** ageing, immigration, and new therapeutic approaches are shaping the epidemiology of virus-related chronic liver disease. In spite of limits due to the incomplete reporting and misclassification of the etiology of liver disease, mortality data represent an additional source of information for surveillance.

关键词： Hepatitis C virus Hepatitis B virus Mortality Epidemiology Immigrants

Microbiological assessment of street foods at the point of sale in Maputo(Mozambique)

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Food Quality and Safety 2021年第1期5卷 22-30页

作者： Acacio Salamandaneu Ana Carla Silva luisa Brito Manuel Malfeito-Ferreira Linking Landscape EnvironmentAgriculture and Food(LEAF)Research CentreInstituto Superior de AgronomiaUniversity of LisbonLisbonPortugal Faculdade de Ciencias de Saude Universidade LurioBairro de MarrereNampulaMozambique

Objectives:The aim of this study was to assess the microbiological quality and safety of street food sold in the main streets and informal markets of Maputo,the capital of *** and Methods:From 83 different vendors selling different types of foods,83 samples of ready-to-eat(RTE)street food were ***,Escherichia coli and total coliforms were used as quality and hygiene *** monocytogenes(***)Salmonella and coagulase-positive staphylococc were used as food safety ***:High proportions of unsatisfactory food samples were found in both traditional hot(76.7%)and cold(75%)*** and Salmonella were tested negative in this ***,when coagulase-positive staphylococci was used as a food safety indicator,approximately 25%(23/83)of the food samples analyzed were classified as unsatisfactory/potentially ***:These results,showing that street food sold in Maputo clearly requires adequate sanitary conditions for its preparation and sale,contribute to the development of good manufacturing practices(GMP)for street food in Maputo,*** is the first report on the microbiological quality and safety of street food in Mozambique.

关键词： ready-to-eat street food Escherichia coli coagulase-positive staphylococcr good manufacturing practices good hygiene practices

Diagnostic strategy for occult hepatitis B virus infection

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World Journal of Gastroenterology 2011年第12期17卷 1553-1557页

作者： Sara Ocana Maria luisa Casas Ingrid Buhigas Jose Luis Lledo Service of Clinical Laboratory Hospital Universitario Fundación AlcorcónAv Budapest-128922 AlcorcónMadrid 28036Spain Service of Biochemistry Hospital Universitario Fundación Alcorcón.Av Budapest-1.28922 AlcorcónMadrid 28036Spain Service of Gastroenterology Hospital Universitario Fundación Alcorcón.Av Budapest-1.28922 AlcorcónMadrid 28036Spain Navarro Service of Gastroenterology Hospital Universitario Fundación AlcorcónAv Budapest-1.28922 AlcorcónMadrid 28036Spain

In 2008,the European Association for the study of the liver(EASL) defined occult hepatitis B virus infection (OBI) as the"presence of hepatitis B virus(HBV) DNA in the liver(with detectable or undetectable HBV DNA in the serum) of individuals testing hepatitis B surface antigen(HBsAg) negative by currently available assays".Several aspects of occult HBV infection are still poorly understood,including the definition itself and a standardized approach for laboratory-based detection,which is the purpose of this *** clinical significance of OBI has not yet been established;however,in terms of public health,the clinical importance arises from the risk of HBV ***,it is important to detect high-risk groups for occult HBV infection to prevent *** main issue is,perhaps,to identify the target population for screening *** is very low or undetectable in occult HBV infection,even when the most sensitive methods are used,and the detection of the viral DNA reservoir in hepatocytes would provide the best evaluation of occult HBV prevalence in a defined set of ***,this diagnostic approach is obviously unsuitable:blood detection of occult hepatitis B requires assays of the highest sensitivity and specificity with a lower limit of detection<10 IU/mL for HBV DNA and<0.1 ng/mL for HBsAg.

关键词： Occult hepatitis B virus infection Hepatitis B surface antigen Hepatitis B virus DNA Anti-HBc

How Sugars Might Coordinate Chloroplast and Nuclear Gene Expression during Acclimation to High Light Intensities

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Molecular Plant 2014年第7期7卷 1121-1137页

作者： Rainer E. Haiusler luisa Heinrichs Jessica Schmitz Ulf-Ingo Flugge Department of Botany II Cologne Biocenter University of Cologne ZuIpicherstr. 47b 50674 Cologne Germany Present address: Plant Molecular Physiology and Biotechnology Heinrich-Heine-University Universitatsstrasse 1 40225 D~sseldorfGermany

The concept of retrograde control of nuclear gene expression assumes the generation of signals inside the chloroplasts, which are either released from or sensed inside of the organelle. In both cases, downstream signaling path- ways lead eventually to a differential regulation of nuclear gene expression and the production of proteins required in the chloroplast. This concept appears reasonable as the majority of the over 3000 predicted plastidial proteins are encoded by nuclear genes. Hence, the nucleus needs information on the status of the chloroplasts, such as during acclimation responses, which trigger massive changes in the protein composition of the thylakoid membrane and in the stroma. Here, we propose an additional control mechanism of nuclear- and plastome-encoded photosynthesis genes, taking advantage of pathways involved in sugar- or hormonal signaling. Sugars are major end products of photosynthesis and their con- tents respond very sensitively to changes in light intensities. Based on recent findings, we ask the question as to whether the carbohydrate status outside the chloroplast can be directly sensed within the chloroplast stroma. Sugars might syn- chronize the responsiveness of both genomes and thereby help to coordinate the expression of piastome- and nuclear- encoded photosynthesis genes in concert with other, more specific retrograde signals.

关键词： acclimation--physiological assimilate allocation/partitioning carbohydrate metabolism molecular physiology membrane proteins Arabidopsis.

Effects of natural mineral-rich water consumption on the expression of sirtuin 1 and angiogenic factors in the erectile tissue of rats with fructose-induced metabolic syndrome

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Asian Journal of Andrology 2014年第4期16卷 631-638页

作者： Cidalia D Pereira Milton Severo luisa Rafael Maria Joao Martins Delminda Neves Department of Biochemistry Faculty of Medicine University of Porto Department of Clinical Epidemiology Predictive Medicine and Public Health Faculty of Medicine University of Porto Department of Clinical Pathology Sao Joao Hospital Centre EPE Department of Experimental Biology Faculty of Medicine and Institute for Molecular and Cell Biology (IBMC) University of Porto Portugal.

Consuming a high-fructose diet induces metabolic syndrome （MS）-Iike features, including endothelial dysfunction. Erectile dysfunction is an early manifestation of endothelial dysfunction and systemic vascular disease. Because mineral deficiency intensifies the deleterious effects of fructose consumption and mineral ingestion is protective against MS, we aimed to characterize the effects of 8weeks of natural mineral-rich water consumption on the structural organization and expression of vascular growth factors and receptors on the corpus cavernosum （CC） in 10% fructose-fed Sprague-Dawley rats （FRUCT）. Differences were not observed in the organization of the CC either on the expression of vascular endothelial growth factor （VEGF） or the components of the angiopoietins/Tie2 system. However, opposing expression patterns were observed for VEGF receptors （an increase and a decrease for VEGFR1 and VEGFR2, respectively） in FRUCT animals, with these patterns being strengthened by mineral-rich water ingestion. Mineral-rich water ingestion （FRUCTMIN） increased the proportion of smooth muscle cells compared with FRUCT rats and induced an upregulatory tendency of sirtuin I expression compared with the control and FRUCT groups. Western blot results were consistent with the dual immunofluorescence evaluation. Plasma oxidized low-density lipoprotein and plasma testosterone levels were similar among the experimental groups, although a tendency for an increase in the former was observed in the FRUCTMIN group. The mineral-rich water-treated rats presented changes similar to those observed in rats treated with MS-protective polyphenol-rich beverages or subjected to energy restriction, which led us to hypothesize that the effects of mineral-rich water consumption may be more vast than those directly observed in this study.

关键词： angiopoietins erectile tissue hypersaline sodium-rich naturally sparkling mineral water receptors sirtuin 1 vascularendothelial growth factor

Unlocking the in vitro anti-Trypanosoma cruzi activity of halophyte plants from the southern Portugal

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Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 714-719页

作者： Marta Oliveira Policarpo Ademar Sales Junior Maria Joao Rodrigues Marina DellaGreca luisa Barreira Silvane Maria Fonseca Murta Alvaro Jose Romanha luisa Custodio Center of Marine Sciences (CCMAR) University of AlgarveCampus de Gambelas 8005-139 FaroPortugal Centro de Pesquisas Rene Rachou FIOCRUZ Belo HorizonteMinas GeraisBrazil Dipartimento di Chimica Organica e Biochimica Universita` Federico Ⅱ Complesso Universitario Monte Sant'AngeloVia Cintia 4I-80126NapoliItaly

Objective:To evaluate the in vitro anti-Trypanosoma cruzi（***） activity of organic extracts prepared from halophyte species collected in the southern coast of Portugal（Algarve）,and chemically characterize the most active ***:Acetone,dichloromethane and methanol extracts were prepared from 31 halophyte species and tested in vitro against trypomastigotes and intracellular amastigotes of the Tulahuen strain of *** most active extract was fractionated by preparative HPLC-DAD,affording 11 *** most selective fraction was fully characterized by ***:From 94 samples tested,one was active,namely the root dichloromethane extract of Juncus acutus(IC50 < 20 μg/mL).This extract was fractionated by HPLC,affording 11 fractions,one of them containing only a pure compound（juncunol）,and tested for anti-parasitic *** 8(IC50 = 4.1 μg/mL) was the most active,and was further characterized by *** major compounds were phenanthrenes,9,10-dihydrophenanthrenes and ***:Our results suggest that the compounds identified in fraction 8 are likely responsible for the observed anti parasitic *** research is in progress aiming to isolate and identify the specific active *** the best of our knowledge,this is the first report on the in vitro anti *** activity of halophyte species.

关键词： Chagas disease Halophytes Trypanosoma cruzi Phenanthrenes Juncunol