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IL-1β, TNF-α and Sambucus nigra Reactive Serum Proteins as Biomarkers of Mild Cognitive Impairment and Alzheimer Disease Progression

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Advances in Alzheimer's Disease 2015年第4期4卷 99-109页

作者： luis Manuel Castillo enrique Moreno Yaneth Rodriguez-Agudelo MireyaChavez-Oliveros Zoila Trujillo Blanca Espinosa Emma Rodriguez-Maldonado luis Felipe Montano Jorge Guevara Departamento de Bioquimica Facultad de MedicinaUniversidad Nacional Autonoma de MexicoMexico CityMexico Departamento de Neuropsicologia Instituto Nacional de Neurologia y NeurocirugiaMexico CityMexico Geriatra Instituto Nacional de Neurologia y Neurocirugia Mexico CityMexico Laboratorio de Bioquimica Instituto Nacional de Enfermedades RespiratoriasMexico CityMexico Departamento de Fisiología Instituto Nacional de CardiologiaMexico CityMexico Departamento de Biologia Celular y Tisular Facultad de MedicinaUniversidad Nacional Autonoma de MexicoMexico CityMexico

Amyloid-β (Aβ) can induce a chronic inflammatory immune response that is associated, amongst many others, to abnormal glycosylation, inducible nitric oxide synthase (iNOS) and nitric oxide (NO). The relation between development of Mild Cognitive Impairment (MCI) and Alzheimer’s disease progression and these serum markers has not been evaluated. Serum levels of iNOs, NO, TNF-α, IL-1β, IL-6, IL-8, IL-10, and IL-12 are determined with commercially available kits. Sialylation of albumin-free serum patterns is determined by Western blot analysis with Sambucus nigra (specific for sialic acid attached to terminal galactose in α2,6 linkage) lectin. Apolipoprotein E (ApoE) haplotype is determined by Western blot using specific anti-ApoE 2, 3 or 4 antibodies. A mini-mental state examination (MMSE) test is also performed in the 10 MCI patients, 19 Alzheimer’s disease (AD) patients and 46 healthy age-matched controls evaluated. The results show an increase of iNOS in MCI and AD but significantly higher NO concentrations are only found in MCI patients. TNF-α and IL-1β concentrations are the only significantly increased cytokines in MCI patients;no differences between control and MCI or AD patients are found in regard to the other cytokines. An abnormal MMSE test result only correlates with a decrease in serum NO concentration in MCI patients. The terminal sialic acid linkage pattern of serum proteins also shows highly significant differences between MCI and AD patient. ApoE3/4 or 4/4 haplotypes are characteristic of MCI and AD patients. Our results imply that increased serum TNF-α, IL-1β, iNOS, NO and alterations of serum proteins glycosylation patterns in adult individuals with an abnormal MMSE test may serve as an early biomarker of MCI and AD development.

关键词： Alzheimer Disease Mild Cognitive Impairment Nitric Oxide Inflammatory Cytokines Glycosylation Apolipoprotein E4

建成环境对步行和自行车出行的影响——以波哥大为例

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城市交通 2016年第5期14卷 83-96页

作者： Robert Cervero Olga L.Sarmiento enrique Jacoby luis Fernando Gomez Andrea Neiman 耿雪加州大学伯克利分校城市和区域规划系美国加利福尼亚州伯克利94720 安第斯大学医学院哥伦比亚波哥大111711 泛美卫生组织美国华盛顿特区20037 艾伯特基金会哥伦比亚协调办公室哥伦比亚波哥大111311 伊利诺伊大学芝加哥分校公共卫生学院美国伊利诺伊州芝加哥60607 中国城市规划设计研究院北京100037

哥伦比亚波哥大以可持续的城市交通系统闻名,包括其密集的自行车道网络以及Ciclovía系统——为骑车者和行人预留的道路空间,可在星期日和节假日休闲出行使用。探讨此类设施以及建成环境的其他属性——城市密度、混合用地、可达性以及... 详细信息

哥伦比亚波哥大以可持续的城市交通系统闻名,包括其密集的自行车道网络以及Ciclovía系统——为骑车者和行人预留的道路空间,可在星期日和节假日休闲出行使用。探讨此类设施以及建成环境的其他属性——城市密度、混合用地、可达性以及与公共交通车站的距离——如何影响步行和自行车出行行为以及Ciclovía的使用。结果表明,道路设施设计要素例如道路密度、连通性、与Ciclovía车道的距离,与体力活动相关;而建成环境的其他属性如密度和用地混合程度,对体力活动影响甚微。这可能是由于波哥大建成区的大部分街区均在非机动交通出行占绝对优势的时期演变发展——紧凑、用地混合、交通可达性水平相似。由此可见,影响非机动交通出行的因素是设施设计而非一般的街区用地属性。

关键词：建成环境自行车出行健康体力活动公共交通步行

rate of adverse events of gastroduodenal snare polypectomy for non-flat polyp is low: A prospective and multicenter study

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World Journal of Gastroenterology 2017年第47期23卷 8405-8414页

作者： Henry Córdova Lidia Argüello Carme Loras Antonio Naranjo Rodríguez Faust Riu Pons Joan B Gornals David Nicolás-Pérez Xavier Andújar Murcia luis Hernández Santos Santolaria Carles Leal Carles Pons enrique Pérez-Cuadrado-Robles Orlando García-Bosch Michel Papo Berger José luis Ulla Rocha Cristina Sánchez-Montes Gloria Fernández-Esparrach Endoscopy Unit.Institut de Malalties Digestives Hospital ClínicIDIBAPSCIBERehdUniversitat de Barcelona Digestive Endoscopy Unit Digestive Diseases DepartmentGastrointestinal Endoscopy Research GroupIISLa Fe Polytechnic University Hospital Hospital Universitari Mútua de Terrassa CIBERehd Hospital Reina Sofía Hospital Del Mar Hospital Universitari de Bellvitge IDIBELLUniversitat de BarcelonaHospitalet de Llobregat Hospital Universitario de Canarias Hospital Santos Reyes Aranda de Duero Hospital San Jorge Consorci Hospitalari de Vic Universitat de Vic Hospital de Viladecans Hospital Morales Meseguer Hospital Moisès Broggi Sant Joan Despí Hospital Joan XXIII Complejo Hospitalario de Pontevedra

AIM To evaluate the rate of adverse events(AEs) during consecutive gastric and duodenal polypectomies in several Spanish centers. METHODS Polypectomies of protruded gastric or duodenal polyps ≥ 5 mm using hot snare were prospectively included. Prophylactic measures of hemorrhage were allowed in predefined cases. AEs were defined and graded according to the lexicon recommended by the American Society for Gastrointestinal Endoscopy. Patients were followed for 48 h, one week and 1 mo after theprocedure. RESULTS308 patients were included and a single polypectomy was performed in 205. Only 36(11.7%) were on prior anticoagulant therapy. Mean polyp size was 15 ± 8.9 mm(5-60) and in 294 cases(95.4%) were located in the stomach. Hemorrhage prophylaxis was performed in 219(71.1%) patients. Nine patients presented AEs(2.9%), and 6 of them were bleeding(n = 6, 1.9%)(in 5 out of 6 AE, different types of endoscopic treatment were performed). Other 24 hemorrhagic episodes could be managed without any change in the outcome of the endoscopy and, consequently, were considered incidents. We did not find any independent risk factor of *** Gastroduodenal polypectomy using prophylactic measures has a rate of AEs small enough to consider this procedure a safe and effective method for polyp resection independently of the polyp size and location.

关键词： Polypectomy Bleeding Adverse events Protruded polyps Gastroduodenal Foregut

Anatomical characterization of central, apical and minimal corneal thickness

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International Journal of Ophthalmology(English edition) 2014年第4期7卷 668-672页

作者： Federico Saenz-Frances Martha Cecilia Bermúdez-Vallecilla Lara Borrego-Sanz luis Jaez J osé Marìa Martinez-de-la-Casa Laura Morales-Fernandez enrique Santos-Bueso Julián Garcia-Sanchez Julián Garcia-Feijoo Department of Ophthalmology Hospital Clínico San CarlosUniversidad Complutense de Madrid Instituto de Tecnologìa del Conocimiento Universidad Complutense de Madrid

·AIM: To anatomically locate the points of minimum corneal thickness and central corneal thickness(pupil center) in relation to the corneal apex.·METHODS: Observational, cross-sectional study, 299 healthy volunteers. Thickness at the corneal apex(AT),minimum corneal thickness(MT) and corneal thickness at the pupil center(PT) were determined using the pentacam. Distances from the corneal apex to MT(MD)and PT(PD) were calculated and their quadrant position(taking the corneal apex as the reference) determined:point of minimum thickness(MC) and point of central thickness(PC) depending on the quadrant position. Two multivariate linear regression models were constructed to examine the influence of age, gender, power of the flattest and steepest corneal axes, position of the flattest axis, corneal volume(determined using the Pentacam)and PT on MD and PD. The effects of these variables on MC and PC were also determined in two multinomial regression models.·RESULTS: MT was located at a mean distance of 0.909 mm from the apex(79.4% in the inferior-temporal quadrant). PT was located at a mean distance of 0.156 mm from the apex. The linear regression model for MD indicated it was significantly influenced by corneal volume(B =-0.024; 95% CI:-0.043 to-0.004). No significant relations were identified in the linear regression model for PD or the multinomial logisticregressions for MC and PC.·CONCLUSION: MT was typically located at the inferiortemporal quadrant of the cornea and its distance to the corneal apex tended to decrease with the increment of corneal volume.

关键词： cornealthickness anatomy glaucoma

Qigong improves balance in young women: a pilot study

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Journal of Integrative Medicine 2013年第4期11卷 241-245页

作者： María Victoria González López-Arza enrique Varela-Donoso Jesús Montanero-Fernández Juan Rodríguez-Mansilla Blanca González-Sánchez luis González López-Arza Medicine and Surgery Department Extremadura University School of Medicine Adolor Research Group of the Extremadura University Physical and Rehabilitation Medicine Department Complutense University School of Medicine Mathematics Department Extremadura University School of Sciences Health Care Centre of Zafra

BACKGROUND： Balance problems are common in people of all ages and can lead to falls, thus causing fractures with consequent disability. Qigong practice has long been part of daily life in Chinese culture, and has good effects on physical health maintenance. OBJECTIVE： The present work describes the change in balance in young, healthy women after practising Qigong for eight weeks. DESIGN, SETTING, PARTICIPANTS AND INTERVENTIONS： The study took the form of a controlled, randomised longitudinal trial, and involved 30 women aged 18-25 years. The subjects had no prior experience of Qigong or Tai Chi and were unaware of the aims of the study. Subjects were randomly assigned to a Qigong intervention group or a control group. Those in the Qigong intervention group performed ＂exercises in 20 figures for health and long-life＂（Wang Ziping） for 1 h twice per week, for 4 weeks. The control group undertook no exercise at all. MAIN OUTCOME MEASURES： The main outcome measure was the stabilometry values. These were obtained in a unipodal support test, using a plantar pressure platform with optical sensors. RESULTS： The Qigong subjects showed a significant improvement in their stabilometry results （40.1% pre-intervention and 56.4% post-intervention）（P〈0.045）, while no improvement was seen in the control group （51.2% pre-intervention and 53.5% post-intervention）. At the beginning of the intervention, the stabilometry values recorded for the Qigong intervention group were worse than those recorded for the control group （40.15% and 51.21% respectively; P=0.121）. However, a comparison of the post-intervention values between these groups showed that these differences have disappeared （P=0.653）. CONCLUSION： Qigong can improve balance in healthy, young women.

关键词： Qigong musculoskeletal equilibrium women pilot projects

Probability of Osteoporotic Vertebral Fractures Assessment Based on DXA Measurements and Finite Element Simulation

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Advances in Bioscience and Biotechnology 2014年第6期5卷 527-545页

作者： enrique López Elena Ibarz Antonio Herrera Jesús Mateo Antonio Lobo-Escolar Sergio Puértolas luis Gracia Aragón Health Sciences Institute Zaragoza Spain Department of Design and Manufacturing Engineering University of Zaragoza Zaragoza Spain Department of Mechanical Engineering University of Zaragoza Zaragoza Spain Department of Orthopaedic Surgery and Traumatology Miguel Servet University Hospital Zaragoza Spain Department of Surgery University of Zaragoza Zaragoza Spain

Osteoporotic vertebral fractures represent major cause of disability, loss of quality of life and even mortality among the elderly population. Decisions on drug therapy are based on the assessment of risk factors for fracture, from bone mineral density measurements. The combination of biomechanical models with clinical studies could better estimate bone strength and support the specialists in their decision. A model to assess the probability of fracture, based on the Damage and Fracture Mechanics has been developed, evaluating the mechanical magnitudes involved in the fracture process from clinical bone mineral density measurements. The model is intended for simulating the degenerative process in the skeleton, with the consequent lost of bone mass and hence the decrease of its mechanical resistance which enables the fracture due to different traumatisms. Clinical studies were chosen, both in non-treatment conditions and receiving drug therapy, and fitted to specific patients according their actual bone mineral density measures. The predictive model is applied in a finite element simulation of the lumbar spine. The fracture zone would be determined according loading scenario (fall, impact, accidental loads, etc.), using the mechanical properties of bone obtained from the evolutionary model corresponding to the considered time. Bone mineral density evolution in untreated patients and in those under different treatments was analyzed. Evolutionary curves of fracture probability were obtained from the evolution of mechanical damage. The evolutionary curve of the untreated group of patients presented a marked increase of the fracture probability, while the curves of patients under drug treatment showed variable decreased risks, depending on the therapy type. The finite element model allowed obtaining detailed maps of damage and fracture probability, identifying high-risk local zones at vertebral body, which are the usual localization of osteoporotic vertebral fractures. T

关键词： Osteoporosis Osteoporotic Vertebral Fracture Predictive Model Finite Elements Fracture Risk Fracture Probability

Gut microbes limit growth in house sparrow nestlings(Passer domesticus)but not through limitations in digestive capacity

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Integrative Zoology 2018年第2期13卷 139-151页

作者： Kevin D.KOHL Antonio BRUN Seth R.BORDENSTEIN enrique CAVIEDES-VIDAL William H.KARASOV Department of Biological Sciences Vanderbilt UniversityNashvilleTennesseeUSA Department of Biological Sciences University of PittsburghPittsburghPennsylvaniaUSA Department of Forest and Wildlife Ecology University of Wisconsin-MadisonMadisonWisconsinUSA Department of Pathology Microbiologyand ImmunologyVanderbilt UniversityNashvilleTennesseeUSA Institute for Multidisciplinary Research in Biology of San luis National Scientific and Technical Research CouncilSan LuisArgentina Department of Biochemistry and Biological Sciences Universidad Nacional de San LuisSan LuisArgentina

Recent research often lauds the services and beneficial effects of host-associated microbes on ***,hosting these microbes may come at a *** example,germ-free and antibiotic-treated birds generally grow faster than their conventional *** the wild,juvenile body size is correlated with survival,so hosting a microbiota may incur a fitness *** altricial nestlings represent an interesting study system in which to investigate these interactions,given that they exhibit the fastest growth rates among vertebrates,and growth is limited by their digestive *** investigated whether reduction and restructuring of the microbiota by antibiotic treatment would:(i)increase growth and food conversion efficiency in nestling house sparrows(Passer domesticus);(ii)alter aspects of gut anatomy or function(particularly activities of digestive carbohydrases and their regulation in response to dietary change);and(iii)whether there were correlations between relative abundances of microbial taxa,digestive function and nestling *** treatment significantly increased growth and food conversion efficiency in *** did not alter aspects of gut anatomy that we considered but depressed intestinal maltase *** were no significant correlations between abundances of microbial taxa and aspects of host ***,we conclude that microbial-induced growth limitation in developing birds is not driven by interactions with digestive ***,decreased energetic and material costs of immune function or beneficial effects from microbes enriched under antibiotic treatment may underlie these *** the costs and tradeoffs of hosting gut microbial communities represents an avenue of future research.

关键词： antibiotics food conversion efficiency gut microbiota host-microbe interactions maltase

Daily expression of sodium-dependent glucose cotransporter-1protein in jejunum during rat ontogeny

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Animal Nutrition 2019年第3期5卷 290-296页

作者： Juan I.Baston Fabricio D.Cid enrique Caviedes-Vidal Juan G.Chediack Laboratorio de Fisiopatología Endometrial Instituto de Biología y Medicina Experimental(IByME)CONICETCiudad Autoonoma de Buenos AiresC1428ADNArgentina Laboratorio de Biología Integrativa“Prof.E.Caviedes Codelia” Facultad de QuímicaBioquímica y FarmaciaUniversidad Nacional de San LuisSan LuisD5700HHWArgentina Instituto Multidisciplinario de Investigaciones en Biología-San luis(IMIBIO-SL CCT-SL)CONICETSan Luis D5700HHWArgentina

It is widely known that intestinal capacities such as the enzymatic hydrolysis of carbohydrates,lipids and proteins,and the subsequent absorption of the hydrolyzed *** evolutionary matched to dietary loads and feeding *** this study,we demonstrate that the protein expression of apically located sodium-dependent glucose cotransporter-1(SGLT-1)throughout rat ontogeny is daily adjusted to afford glucose uptake when the load of this metabolically essential monosaccharide in the intestinal lumen is *** jejunal expression of SGLT-1 protein in 14 one-day-old suckling pups was found to increase at dark and early light phase(P<0.05).when they have a better access to mother *** weaning21-d-old and juvenile 28-d-old rats,the cotransporter expression was high throughout the entire day(P<0.05).Finally,adult 90-d-old rats showed a well-developed circadian rhythm for SGLT-1 protein(P<0.05).whose expression increased at late light and dark phase when the highest intestinal glucose load was *** our knowledge,these results are the first reporting the daily profile of SGLT-1 expression during rat early developmental stage and may contribute to understand the biological significance of a well-established molecular capacity to deal with the crucial increase of glucose load in the diet during the weaning process.

关键词： Intestinal SGLT-1 Protein expression Daily rhythms Ontogeny Rat

Zinc and Lead Biosorption by Delftia tsuruhatensis: A Bacterial Strain Resistant to Metals Isolated from Mine Tailings

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Journal of Water Resource and Protection 2012年第4期4卷 207-216页

作者： Dorian A. Bautista-Hernández Landy I. Ramírez-Burgos enrique Duran-Páramo luis Fernández-Linares Faculty of Chemistry Programa de Ingeniería Química Ambiental y Química Ambiental PIQAyQA Universidad Nacional Autónoma de México México City México Laboratory of Bioprocesses Department of Bioprocesses Unidad Profesional Interdisciplinaria de Biotecnología UPIBI-Instituto Politécnico Nacional México City México Rearch Centre for Environmental Quality Instituto Tecnológico de Estudios Superiores de Monterrey Campus Estado de México Atizapán de Zaragoza México

A bacterial strain capable of Zinc and Lead biosorption was isolated from mine tailings. This strain showed the highest minimum inhibitory concentrations (MIC) of metals among other isolates in metal-resistance tests. Sorption tests were conducted placing 0.015 g of dry biomass in 10 ml of metallic solution at fixed pH. Contact was analyzed at different times (kinetics) and different initial concentrations (isotherm). The biomass was separated by centrifugation and the concentration of non-absorbed metal was determined using atomic absorption spectroscopy. The strain was identified by 16S sequencing as Delftia tsuruhatensis. The order of toxicity of the metals to the bacterium was Zn > Pb > Se > Ni > Cu = Al. Zinc and Lead absorption kinetics were adjusted to the pseudo second order equation (r2 = 0.99), showing that equilibrium was reached at 40 and 20 min, respectively. Maximal absorption of Pb and Zn was 0.216 and 0.207 mmol?g–1, respectively;which can be considered a median magnitude capacity when compared to other biosorbents described in the literature.

关键词： Biosorption Langmuir and Freundlich Isotherms Delftia Tsuruhatensis Pb(II) Zn(II)