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化学与微生物实验室

现代装饰 2024年第3期 62-69页

作者： Jose luis Esteban Penelas(设计) Yolanda Hernandez Lorente(设计) maria Esteban Casanas(设计) Antonio guijarro(设计) marina Rodriguez Sotoca(设计) Kevin Roman Cruz(设计) Yakaterina Savina(设计) Imagen Subliminal(摄影) 舒禹(编译) 不详 miguel de guzman+Rocio Romero

建筑呈现明显的后工业美学特征,内设有一个微生物和化学分析实验室,功能设施极为复杂,使用中需要极高的功能效率与超先进的图像兼容。设计重新激活地区周围的强大工业景观,通过穿孔立面,过滤环境并生成独特的室内景观。

关键词：微生物实验室工业景观室内景观功能设施美学特征

Effect of Different Reproductive Stages and Culture Times on Domestic Cat <i>in Vitro </i>Oocyte Maturation

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Advances in Reproductive Sciences 2021年第2期9卷 129-138页

作者： José Ernesto Hernández Pichardo miguel Ramses Del Moral Reyes Michael E. Kjelland José luis Rodríguez Suastegui Department of Agricultural and Animal Production Division of Biological and Health SciencesMetropolitan Autonomous University-Xochimilco Mexico City Mexico Private Clinic Mexico City Mexico Conservation Genetics & Biotech LLC Valley City USA Biological and Health Sciences PhD Program Metropolitan Autonomous University-Xochimilco Mexico City Mexico

The domestic cat has been used as a model to carry out comparative research in assisted reproduction, to be applied in wild cats. The efficiency in domestic cat IVM concerning the reproductive status and/or cultivation times has previously been investigated;however, the studies were carried out separately. The objective of this research was to evaluate the maturation of oocytes of domestic cats of different reproductive stages using two different in vitro culture times. The ovaries were obtained by Ooforo-Salpingo-Hysterectomy of cats that were of the following groups: 1) prepubertal, 2) follicular, 3) pregnant or 4) in anestrus. Maturation was carried out with TCM199 medium supplemented with BSA for 24 h and 48 h. On average, 29 ± 25, 20 ± 15, 17 ± 9 and 17 ± 13 oocytes/cat were recovered from the prepubertal follicular, pregnant, and anestrus stages, respectively, but did not show a significant difference (P > 0.05). Also, meiotic maturation did not show a significant difference between the different reproductive stages at 24 h and 48 h, respectively (P > 0.05). However, in the prepubertal and follicular stages, greater oocyte maturation numbers were observed at 48 h compared to 24 h (P result was not observed in the pregnant and anestrus stages (P > 0.05), indicating that the in vitro culture duration is an important factor during in vitro maturation of domestic cat oocytes.

关键词： Domestic Cat Oocytes In Vitro Maturation Culture Time Estrus Cycle Stages

Patch isolation and shape predict plant functional diversity in a naturally fragmented forest

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Journal of Plant Ecology 2018年第1期11卷 136-146页

作者： Amaranta Arellano-Rivas J.Arturo De-nova miguel A.Munguía-Rosas Instituto de Investigación de Zonas Desérticas Universidad Autónoma de San Luís PotosíSan Luis Potosí78377México Laboratorio de Ecología Terrestre Departamento de Ecología HumanaCentro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional(CINVESTAV)Mérida 97310México Facultad de Agronomía Universidad Autónoma de San Luís PotosíSan Luis Potosí78321México

Aims It is known that taxonomic diversity can be predicted by the spatial configuration of the habitat,in particular by its area and degree of ***,taxonomic diversity is a poor predictor of ecosystem *** functional diversity is strongly linked to the func-tionality and stability of ecosystems,little is known about how changes in the spatial configuration of the habitat affect functional *** this study,we evaluated whether the spatial configuration of forest patches predicts the functional diversity of plants in a fragmented *** Five functional leaf traits(leaf dry matter content,leaf punch force,specific leaf area,leaf size and leaf thickness)were measured for 23 dominant plant species in 20 forest patches in a naturally fragmented forest on the yucatan ***-weighted multivariate and individual trait metrics of functional diversity were calculated and cor-related with size,degree of isolation and the shape of forest *** Findings Patch shape was negatively correlated with multivariate and individ-ual trait(leaf dry matter content and leaf size)metrics of functional *** isolation measures were also negatively correlated with individual trait(leaf dry matter content,leaf punch force and leaf size)metrics of functional *** other words,greater patch shape irregularity and isolation degree impoverish plant func-tional *** is the first report of the negative effects of patch shape irregularity and isolation on the functional diversity of plant communities in a forest that has been fragmented for a long time.

关键词： forest fragmentation functional diversity habitat spatial configuration petenes yucatan

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食用疫苗

科学（中文版） 2005年第7期 10-11页

作者： luis miguel Ariza 聂辉成

为世界上的贫穷人口接种疫苗,没有冷藏设备仍然是一个恼人的难题,因为药品在高温下常常失去效力.在200年前,一个解决方案就是在孤儿身上培育疫苗(见左栏)."那真是一个有趣的创意."亚利桑那生物设计研究所创始人Charles Arntzen说,但这... 详细信息

为世界上的贫穷人口接种疫苗,没有冷藏设备仍然是一个恼人的难题,因为药品在高温下常常失去效力.在200年前,一个解决方案就是在孤儿身上培育疫苗(见左栏)."那真是一个有趣的创意."亚利桑那生物设计研究所创始人Charles Arntzen说,但这种方法在今天行不通了.不过,Arntzen认为可以在作物里保存食用疫苗.

关键词：食用疫苗接种疫苗冷藏设备 200年解决方案创始人研究所作物桑

Design and Experimentation of a 1 MW Horizontal Axis Wind Turbine

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Journal of Power and Energy Engineering 2014年第1期2卷 9-16页

作者： miguel Toledo Velázquez marcelino Vega Del Carmen Juan Abugaber Francis luis A. Moreno Pacheco guilibaldo Tolentino Eslava Instituto Politécnico nacional Laboratorio de Ingeniería Térmica e Hidráulica Aplicada México D.F. México Instituto Politécnico nacional Laboratorio de Ingeniería Térmica e Hidráulica Aplicada México D.F. México.

In this work was carried out the aerodynamics design of a 1 MW horizontal axis wind turbine by using blade element momentum theory (BEM). The generated design was scaled and built for testing purposes in the discharge of an axial flow fan of 80 cm in diameter. Strip theory was used for the aerodynamic performance evaluation. In the numerical calculations was conducted a comparative analysis of the performance curves adding increasingly correction factors to the original equation of ideal flow to reduce the error regarding real operating values got by the experimental tests. Correction factors introduced in the ideal flow equation were the tip loss factor and drag coefficient. BEM results showed good approximation using experimental data for the tip speed ratio less than design. The best approximation of the power coefficient calculation was for tip speed ratio less than 6. BEM method is a tool for practical calculation and can be used for the design and evaluation of wind turbines when the flow rate is not too turbulent and radial velocity components are negligible.

关键词： Wind Turbine Design BET BEM Tip Speed Ratio Tip Lost Factor HAWT

Efficiency of RAPD in assessing genetic variation in commercially viable local varieties of blueberry (<i>Vaccinium</i>)

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Agricultural Sciences 2013年第5期4卷 238-243页

作者： Christian Mandiola Quililongo Pamela Villouta Wheeler luis Velásquez Cumplido miguel Ríos Ramírez Jorge Escobar Fica Departamento de Biología Avda Libertador Bernardo O'Higgins Facultad de Química y Biología Estación Central Universidad de Santiago de Chile Santiago Chile Instituto de Química Laboratorio de Química-Biológica Pontificia Avda Universidad Católica de Valparaíso Facultad de Ciencias Valparaíso Chile Universidad Andrés Bello Facultad de Medicina Center for Integrative Medicine and Innovative Science Santiago Chile

The growth in both production and plantation surface area of blueberries in Chile has been positive, with an estimated surface area of 13,057 hectares (16.9% planting surface) and production in the order of 56,000 tons (16.3% mundial production). Of all the different species of blueberry there are several varieties that have advantages with regard to certain conditions;for example, one variety adapts better to the climate of a particular area, while others are better for early or late harvests, they can also be resistant to fungus, bacteria, etc. Companies are very interested in this issue because prior knowledge of such data can bring financial benefits. Blueberries have a significant degree of morphological variation, which enables recognition of different clones with different environmental adaptation characteristics, but it also allows us to discriminate between different levels of fruit quality, which is of commercial interest. However, these morphological characteristics are late in their expression, making it impossible to recognize the clones in the early stage of their development. A more efficient tool to be able to recognize different clones is the use of molecular markers. Of the techniques based the RAPD (randomly amplified polymorphic DnA). They have the advantage that the method has no need for prior knowledge of the DnA sequence. It is of great interest to the private sector to have prior access to information on the types of clone they possess, in order to then be able to differentiate the varieties, but for this it is necessary to obtain a quick and economical technique. In this study, through the use of RAPD-PCR, it is possible to differentiate between different varieties of Vaccinium grown in Chile in order to then optimize blueberry production in terms of time and resources.

关键词： Blueberry RAPD genetic Variation Molecular marker

Bioclimate-Vegetation Interrelations along the Pacific Rim of north America

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American Journal of Plant Sciences 2012年第10期3卷 1430-1450页

作者： Manuel Peinado gustavo Díaz José Delgadillo Francisco Manuel Ocana-Peinado miguel Angel Macías Juan luis Aguirre Alejandro Aparicio Instituto Universitario de Investigación en Estudios norteamericanos“Benjamin Franklin” Universidad de AlcaláAlcaláde HenaresSpain Facultad de Ciencias Universidad Autónoma de Baja CaliforniaEnsenadaMexico Departamento de Estadística e Investigación Operativa Universidad de GranadaGranadaSpain Departamento de Ciencias Ambientales Universidad de GuadalajaraGuadalajaraMexico

This study was designed to examine relationships between climate and vegetation of the Pacific rim of north America, from the Mediterranean deserts of California to Alaska’s boreal taiga. Relations were inferred from temperature and rainfall data recorded at 457 weather stations and by sampling the vegetation around these stations. Climate data were used to construct climatograms, calculate forty one variables and detect main latitudinal and longitudinal gradients. In order to identify the best functions able to relate our variables, polynomial and non-polynomial regressions were performed. The k-means algorithm was the clustering method used to validate the variables that could best support our bioclimatic classification. The variable that best fitted our classification was finally used to prepare a discriminatory key for bioclimates. Across this extensive area three macrobioclimates were identified, Mediterranean, Temperate and Bo- real, within which we were able to distinguish nine bioclimates. Finally, we relate the different types of potential natural vegetation to each of these bioclimates and describe their floristic composition and physiognomy.

关键词： Bioclimatology Boreal Forests Mediterranean Vegetation Plant Formations Temperate Rainforests Zonobiomes

The interplay of nurse and target plant traits influences magnitude and direction of facilitative interactions under different combinations of stress and disturbance intensities in Andean dry grassland

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Journal of Plant Ecology 2016年第3期9卷 296-310页

作者： Andrea Catorci Luca Malatesta Jose luis Velasquez Federico maria Tardella Horacio Zeballos School of Biosciences and Veterinary Medicine University of Camerinovia Pontoni 5CamerinoMCItaly School of Advanced Studies PhD Course in Environmental Sciences and Public Health University of Camerinovia Lili 55CamerinoMCItaly Centro de Estudios y Promoción del Desarrollo(Desco) Málaga Grenet 678ArequipaPeru Instituto de Ciencias de la naturaleza Territorio Yenergia RenovablesPontificia Universidad Católica del Perù.Av.Universitaria 1801San Miguel 32LimaPerù

Aims Facilitation is a key process in vegetation dynamics,driving the response to natural and anthropogenic *** harsh-grazed systems,palatable plants mainly survive when nested under unpalatable tussocks and *** magnitude and direction of positive interactions are driven by resource availability,extent of herbivory and type of nurse *** hypothesized that different combinations of disturbance and environmental stress affect community composition in the dry Puna(southern Peruvian Andes)by modifying nurse types and plant interactions in magnitude and specific *** investigated whether different combinations of stress and disturbance influence species richness,type and frequency of occurrence of nurse and beneficiary species and magnitude and patterns of plant interactions;whether nurse species influence these interactions and target species change their interactions under different combinations of stress and disturbance and whether plant functional traits differ in the studied communities and influence the pattern of spatial *** We selected three plant communities subject to different precipitation and management regimes:in each we laid a number of transects proportional to its *** collected include species presence/absence,type of spatial interactions with nurse species and functional *** calculated species richness and rarefaction patterns,described the patterns of plant-plant spatial interactions and investigated the associations between nurse and other species in the three communities using indicator species analysis(ISA).We performed ISA and correlation analysis to investigate whether plant functional traits influenced facilitative *** Findings We found that different combinations of stress and disturbance shaped a complex set of responses,including changes in the nurse species *** composition influenced magnitude and direction of plant interactions under different stress

关键词： dry Puna facilitation functional traits nurse and beneficiary species species-specific interaction stress-gradient hypothesis

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Modelling the User

Computer Technology and Application 2013年第7期4卷 327-334页

作者： Evangelos Bekiaris maria gemou Karel Van Isacker Karin Slegerst Lieve Laporte nicky Salmon luis miguel Bascones Mercedes Turrero Ivan Carmona Centre for Research and Technology Hellas (CERTH) Hellenic Institute of Transport (HIT) Thermi Thessaloniki 57001 Greece European Platform for Rehabilitation (EPR) Hellenic Institute of Transport (HIT) Brussels B-1000 Belgium Centre for User Experience Research (CUO) K. U. Leuven Leuven 3605-3000 Belgium Social Research Department Technosite-Fundacion ONCE Madrid 28037 Spain

The relatively high percentage of people with disabilities in Europe combined with the facts of ageing population, strong relation of impairment to age, and as State of the Art shows, dissatisfaction or even unawareness of people with disabilities of available assistive technology are revealing the necessity to incorporate a user-centric approach that beyond 2nd generation practices will achieve to provide embedded and built-in accessibility solutions, as well as toolkits for developers, for ＂engraving＂ accessibility in existing and emerging mass-market ICT-based products, aiming to make accessibility open, plug and play, personalised and configurable, realistic and applicable in various contexts, keeping always the user in the loop. The AEgIS （Accessibility Everywhere： groundwork, Infrastructure, Standards） IP （Integrated Project） of the 7th European Framework Programme seeks to determine whether 3rd generation access techniques will provide a more accessible, more exploitable and deeply embeddable approach in mainstream ICT （information and communication technologies）. This paper presents the holistic UCD （user-centered design） implementation plan, upon which AEgIS has been based in order to achieve its targets, starting from modelling its target users, in the most efficient way possible.

关键词： Accessibility open source UCD mobile applications desktop applications RIA (rich internet applications) people withdisabilities.