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Advances in Microbiology 2012年第3期2卷 268-276页

作者： Cynthia Barbosa Rustiguel Joao Atílio Jorge luis henrique souza guimaraes Department of Biology Faculdade de FilosofiaCiencias e Letras de Ribeirao PretoUniversidade de Sao PauloSao PauloBrazil

Entomopathogenic fungi, such as Metarhizium anisopliae, are able to control various insect pests. These fungi attack the integument of the host using an enzymatic complex. Among the enzymes found in this complex, chitinase is an important component. However, the relation between the chitinase production and the virulence from different M. anisopliae strains has not been analyzed. In this manuscript it is presented the chitinase production by four M. anisopliae strains with different potential of virulence in Solid-State Fermentation using silkworm chrysalis as substrate. The higher chitinase level was obtained with the strain IBCB 360 (7.14 U/g of substrate) with potential virulence of 68% on Diatrea saccharalis. The enzyme production was optimized for all strains using a factorial planning (CCRD) considering the cultivation time and medium humidity as independent variables. The maximal production of chitinase was obtained at a range from 8 to 12-days old cultures and from 45% to 62% of moisture according to the surface response plot, with high R2 value. The enzyme production by the strain IBCB 167 was increased two-folds under optimized conditions, while for the strains IBCB 360 and 425 the chitinase production was increased four-folds and nine-folds for the strain IBCB 384.

关键词： Entomopathogenic Fungus Metarhizium anisopliae Chitinase Solid-State Fermentation

Production of Invertases by Anamorphic(Aspergillus nidulans)and Teleomorphic(Emericela nidulans)Fungi under Submerged Fermentation Using Rye Flour as Carbon Source

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Advances in Microbiology 2013年第5期3卷 421-429页

作者： Juliana Nunes e Oliveira Alves Joao Atílio Jorge luis henrique souza guimaraes Department of Biology Faculty of PhylosophySciences and Letters of Ribeirao PretoUniversity of Sao PauloSao PauloBrazil

The production of invertases by anamorph (A. nidulans) and teleomorph (E. nidulans) was investigated. The best level of extracellular enzymatic production for anomorph was obtained in Khanna medium containing sucrose as carbon source, whereas for teleomorph the best production was archived using M5 medium containing inulin as carbon source. Despite this, rye flour was selected as carbon source. The extracellular enzyme production was higher for teleomorph than that observed for anomorph for all carbon sources used. The enzyme production was inhibited by the addition of fructose and glucose in the medium containing rye flour as carbon source. The best conditions to recover the higher enzymatic activity were temperature of 54℃ - 62℃ and pH of 4.8 5.6 for both enzymes determined by experimental design (CCRD). The stability of the temperatures at 40℃ and 50℃were similar for both enzymes. The invertases from the anomorph and teleomorph were activated by Mn2+, but the response of each one towards the presence of this cation was different with best activation observed for the anomorph enzyme (+80%). The extracellular enzymes were able to hydrolyze inulin, sucrose and raffinose. However, the affinity was higher for sucrose than inulin. In conclusion, the carbon source assimilation and the invertase production, as well as the enzymes properties, were different for the anomorph and teleomorph mycelia.

关键词： Invertase Fructofuranosidase Aspergillus Emericela

The Optimization of Aspergillus ***4 Tannase Production under Submerged Fermentation

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Advances in Microbiology 2014年第3期4卷 143-150页

作者： Alessandra Goncalves de Melo Rayssa Cristina Faria Pedroso luis henrique souza guimaraes José Guilherme Lembi Ferreira Alves Eustáquio souza Dias Mário Lúcio Vilela de Resende Patrícia Gomes Cardoso Department of Biology Sector of MicrobiologyFederal University of LavrasLavrasBrazil Department of Biology University of Sao PauloRibeirao PretoBrazil Department of Food Science Federal University of LavrasLavrasBrazil Department of Phytopathology Federal University of LavrasLavrasBrazil

Tannase is a hydrolytic enzyme that is involved in the biodegradation of tannins and it has biotechnological potential in the pharmaceutical, chemical, food and beverage industries. Microorganisms, especially filamentous fungi, are important tannase producers. The aims of this work were to find a potential tannase producer and to improve the cultivation conditions. Three Aspergillus species (A. japonicus 246A, A. tamarii 3 and Aspergillus sp. GM4) were investigated in different culture media (Adams, Czapeck, Khanna, M5 and Vogel) and inducers (1% and 2% tannic acid;1% green tea;1% methyl gallate;1% gallic acid). Aspergillus sp. GM4 and Adams medium were selected. The tannase production by Aspergillus sp. GM4 in Adams medium was induced in the presence of 2% (w/v) tannic acid and gallic acid as carbon sources, while green tea was not able to induce tannase production. The Plackett-Burman screening design was performed with the variables MgSO4, KH2PO4, yeast extract, tannic acid, agitation rate and salt solution. The variables MgSO4 and agitation rate were selected for the optimization of tannase production using a Central Composite Rotatable Design. Under optimized conditions, a 2.66-fold increase in the enzyme production was observed with small modifications in the medium composition.

关键词： Aspergillus sp. Response Surface Methodology Submerged Fermentation Tannase Tannic Acid

Development of Junction Elements from Study of the Bionics

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Journal of Bionic Engineering 2007年第1期4卷 41-46页

作者： Wilson Kindlein Junior luis henrique Alves Cndido André Canal Marques Sandra souza dos Santos Maurício da Silva Viegas Laboratory of Design and Material Selection-LdSM-Materials Department (DEMAT) School of Engineering–EE Federal University of Rio Grande do Sul-Brazil-UFRGS Brazil

The applications of bionic methodology developed by the Laboratory of Design and Material Selection as basis in the creation of junction elements were demonstrated. These elements favor the application of Ecodesign in reference to the effectiveness of product dismount aiming the reduction of ambient impact in all its phases of use. The creation, the development and the confection of new junction elements were described, and case studies of new products developed specifically with this purpose were presented.

关键词： bionics Ecodesign junction elements

Alendronate disturbs femoral growth due to changes during immunolocalization of transforming growth factor-β1 and bone morphogenetic protein-2 in epiphyseal plate

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World Journal of Experimental Medicine 2020年第1期10卷 1-9页

作者： Juliana souza Vieira Emanuelle Juliana Cunha Juliana Feltrin de souza luis henrique Koeler Chaves Jessica Lakes de souza Allan Fernando Giovanini Clinical Dentistry University PositivoCuritibaPR 81280330Brazil Stomatology Department University Federal of ParanáCuritibaPR 80210-170Brazil

BACKGROUND The epiphyseal growth plate is an important anatomical segment localized on the ends of a long *** the abovementioned atractive reasons for alendronate’s use,few data on the effect of alendronate during epiphyseal growth *** Verify the effect of alendronate on the growth epiphyseal plate,and compare its effect with the size of the femur during the double-staining of the immunolocalization of transforming growth factor-β1(TGF-β1)and bone morphogenetic protein-2(BMP2)in endochondral ossifing in specimens that have received *** Forty newborn rats were randomly divided into two groups:a control group(were given applications of 1 mg/kg physiologic saline)and a group that received Alendronate(a dose of 2.5 mg/kg).These groups were then divided into two subgroups for euthanasia in two and 12 d of *** euthanasia,the femurs were removed,and the femoral bones were measured linearly between the apex of the greater trochanter until the lower intercondylar midlle face to verify the probable bone growth between 3 and 12 d in control and alednroanto treated ***,the surgical pieces were also sent to the histopathology laboratory to produce histological *** obtained slides were stained with hematoxylin and eosin to measure each of the cartilage zones in endochondral *** other slides were immunohistochemically tested for anti-TGF-β1 and BMP-2 antibodies to investigate the immunolocalization of these proteins in the epiphyseal plaque *** On the third day,some diferences between the control group and specimens treated with alendronate were ***,we found similarities in size between the femoral bones when we compared the control group with the specimens that received *** the 12^th day,the bone size of the mice receiving the drug was significantly smaller than those of the control *** results coincide with changes in the TGF-β1 and BMP-2 *** the speci

关键词： Alendronate Bone development Epiphyseal plate Bone morphogentic protein-2 Transforming growth factor-β1

Microstructural characterization and mechanical behavior analysis of 7075-T6 aluminum subjected to simulated lightning strikes

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Chinese Journal of Aeronautics 2021年第12期34卷 39-50页

作者： luis henrique SANTOS Weslei Patrick Teodosio SOUSA Sara Silva Ferreira DE DAFE Pedro Americo Magalhaes JUNIOR Department of Mechanical Engineering Pontificia Universidade Catolica de Minas GeraisBelo Horizonte 30535-901Brazil

Aircrafts damages caused by lightning strikes have been known since the early days of ***,the physical effects on the aircraft structure are still being *** work seeks to evaluate the lightning strike effects in the aluminum alloy *** were submitted to lightning strike simulation in laboratory and the damages evaluated through characterization *** and profilometry tests have shown material loss to 0.272 mm depth in the damaged *** addition,it was detected the material accumulation occurrence in the damage vicinity of the *** the damage,it was found a region where metallurgical changes were *** tensile and microhardness tests results have shown reduction in the percentage elongation and hardness increasing in the material affected by *** results are corroborated by the X-Ray Diffraction(XRD)and Rietveld Method(red line)that indicated an increasing in dislocation density and micro-deformation in the material *** microscopy results have shown the presence of microcracks on the normal and cross-section surface of the samples *** Energy Dispersive X-Ray Spectroscopy(EDXS)and Electron Backscattered Diffraction Test(EBSD)found coarse intermetallic phases and precipitates compounds with dimensions greater than 1 lm in *** were responsible for nucleation of the microcracks that propagate along the material grain boundaries.

关键词： Aircraft structure Characterization techniques Lightning strikes Precipitates Microcracks

Microalbuminuria in White and Black Hypertensive Nondiabetic Brazilian Patients

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World Journal of Cardiovascular Diseases 2014年第5期4卷 236-243页

作者： Andreza Alves Belo Eduardo luis guimaraes Machado Amintas Fabiano de souza Figueiredo Estevao Lanna Figueiredo Centro de Desenvolvimento Inovacao Ciencia e Tecnologia—CDICTBHTECBelo HorizonteMGBrazil Faculdade de Ciencias Medicas de Minas Gerais Belo HorizonteMGBrazil Departamento de Análises Clinicas e Toxicologicas Faculdade de FarmaciaUniversidade Federal de Minas GeraisBelo HorizonteMGBrazil Hospital Lifecenter Belo HorizonteMGBrazil

Microalbuminuria (MAU) is a predictor of cardiovascular mortality in patients with diabetes mellitus (DM) and hypertension (HTN) and also in an unselected population. The American Diabetes Association (ADA) and the National Kidney Foundation (NKF) define MAU as an albumin/creatinine ratio (ACR) between 30 and 300 μg/mg in both men and women. Aim: To evaluate the possible relationship among MAU, HTN and gender and ethnicity in Brazilian nondiabetic primary hypertensive patients. Design: Population-based study. Participants: Ninety-eight men and women, seventy-two black and twenty-six white nondiabetic primary hypertensive patients aged 20 years or older were selected. Forty healthy individuals, paired according to age, gender, and ethnics were used as controls. Methods: Early-morning midstream urine was used. Urinary albumin was spectrophotometrically measured with Coomassie Brillant Blue G-250. Creatinine was determined by a method based on Jaffe’s reaction. ACR (μg albumin/mg creatinine) was calculated. Data are expressed as medians. Results: ACR level was significantly higher in 98 hypertensive patients (38.00) than in 40 control individuals (23.00) (P < 0.001). ACR level was significantly higher in 48 hypertensive male (46.00) than in 50 hypertensive female (34.00) (P = 0.008). No significant effect of ethnicity on ACR levels between 26 hypertensive Whites (35.50) and 72 hypertensive Blacks (38.00) was observed (P = 0.978). Conclusions: The ACR level, significantly higher in hypertensive patients than in control individuals, supports data from the literature. To our knowledge, this is the first study demonstrating that the ACR level is significantly higher in men than in women. The lack of an ethnicity effect supports what was already asserted, namely, that in Brazil, at an individual level, color, as determined by physical evaluation, is a poor predictor of genomic African ancestry, as estimated by molecular markers.

关键词： Albumin Creatinine Rate Microalbuminuria Primary Hypertension Ethnic Groups Brazilian

Production and Partial Characterization of an Extracellular Phytase Produced by Muscodor sp. under Submerged Fermentation

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Advances in Microbiology 2016年第1期6卷 23-32页

作者： Natálie Martins Alves luis henrique souza Guimarães Roberta Hilsdorf Piccoli Patrícia Gomes Cardoso Department of Biology Sector of Microbiology Federal University of Lavras Lavras Brazil Department of Biology University of Sao Paulo Ribeirao Preto Brazil Department of Food Science Federal University of Lavras Lavras Brazil

In most of the raw materials of plant origin used in animal feed, a portion of the phosphorus is stored as phytic acid or phytate. Phytate is the main storage form of phosphorus in vegetables but is not readily assimilated into food at low concentrations of the enzyme phytase. In addition to making phosphorous unavailable, phytate binds divalent cations such as calcium, copper, magnesium, iron, manganese and zinc, preventing the absorption of these nutrients in the gut of the animal. Phytase promotes the hydrolysis of the phytate phosphorus-releasing molecule, thereby increasing its bioavailability in feed. Phytase is distributed in plant and animal tissues and it is synthesized by some species of bacteria and fungi. The addition of this enzyme in the diet of animals is essential to promote greater uptake of phosphorus and also contributes to a decrease in the levels of phosphorus excreted by animals, thus reducing the pollution caused by excess phosphorus in the environment. This work aimed to select a fungus that stands out in the production of phytase among 100 isolates from Brazilian caves belonging to the genera Aspergillus, Penicillium and Cladosporium and 13 endophytic fungi of the aerial part of the coffee plant. For selection, the fungi were cultured in medium containing phytic acid as a sole source of phosphorus. After seven days at 25 °C, we evaluated growth and enzyme production by the presence of the phytic acid halo degradation (Enzymatic Index-EI) surrounding the colonies. Forty-seven species produced phytase, and the fungi Penicillium minioluteum (CF279) and Muscodor sp. (UBSX) showed higher degradation halos, 2.41 and 4.46, respectively. Considering the Muscodor sp. as the main source of phytase, high enzymatic levels were obtained when the fungus was grown under submerged fermentation with initial pH of 5.0 using wheat bran as additional carbon source for 144 h, at 125 rpm and 30 °C. Additionally, the enzyme was stable at pH 5.0 and 40 °C, and inhi

关键词： Endophytic Fungus Muscodor sp. Phosphorus Phytase Phytic Acid

Intestinal Obstruction Associated with Pharmacobezoar in a Patient with Crohn’s Disease: A Case Report

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Surgical Science 2023年第7期14卷 469-473页

作者： Marina Gabrielle Epstein Gabriel Garbato Gabriel Maccapani Camille Diem Benatti Ivan Carlos Batista luis henrique Barreto Chaves Amanda Domit Dall’Alba Vila Nova Star Hospital S&#227o Paulo Brazil Albert Einstein Medical School S&#227o Paulo Brazil

Crohn’s disease might result from a complex interplay between genetic susceptibility, environmental factors, and altered gut microbiota, leading to dysregulated innate and adaptive immune responses. The reported case presents a patient with intestinal subocclusion associated with a pharmacobezoar who had a good evolution with clinical treatment. This case report aims to demonstrate the complexity of diagnostic search, even with the classic version of the disease. And it also comes to show the need for a thorough medical history and differential diagnosis investigation.

关键词： Intestinal Obstruction Pharmacobezoars Crohn’s Disease

Germination,Vigor of Seeds and Emergence of Fabaceae Seedling in Bovine Faeces

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American Journal of Plant Sciences 2014年第14期5卷 2170-2177页

作者： Renata Vianna Lima henrique Duarte Vieira Francielle de souza guimaraes Nardele Moreno Rohem Jr. Tatiana Oliveira da Silva Alberto Magno Fernandes Cynthia Pires guimaraes Amanda Justino Acha Bruno Borges Deminicis Department of Fitotecnia State University of North Fluminense Darcy Ribeiro(UENF)Campos dos GoytacazesRio de JaneiroBrazil Department of Zootecnia State University of North Fluminense Darcy Ribeiro(UENF)Campos dos GoytacazesRio de JaneiroBrazil

This study was conducted to evaluate the physiological quality of forage seeds after passage through the gastrointestinal tract of cattle, as well as the emergence of seedlings derived from these seeds. Three Fabaceae species were used: tropical kudzu, leucaena and calopo. One hundred grams of seeds of each species were offered, separately, to five bovines. After the start of the dispersion, the faeces of the animals were collected every 6 hours, in a total of 72 hours, being taken to the greenhouse immediately after each collection. The study evaluated the total number of seedlings in the stool, the lengths of roots and aerial parts, as well as the natural and dry seedling weight thirty days after the disposal of the faeces in the greenhouse. The experimental design consisted of randomized blocks, with twelve treatments (sampling periods) and five replications (cattle). The best performance for the total number of seedlings in the faeces of cattle was observed in the kudzu species. The length of the root, length of the aerial part and dry matter seedling weight was observed in the leucaena species, followed by kudzu. The calopo showed low results for all variables due to the high degradation of the seeds.

关键词： Pueraria phaseoloides Leucaena leucocephala Calopogonium mucunoides Dispersal Physiological Quality