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Maize Gene Regulatory Network for Phenolic Metabolism

Molecular Plant 2017年第3期10卷 498-515页

作者： Fan Yang Wei Li Nan Jiang Haidong Yu Kengo Morohashi Wilberforce Zachary Oumal daniel E. Morales-Mantilla Fabio Andres Gomez-Canol Eric Mukundi luis daniel prada-salcedo Roberto Alers Velazquez Jasmin Valentin Maria Katherine Mejia-Guerra John Gray Andrea I. Doseff Erich Grotewold Center for Applied Sciences (CAPS) Department of Molecular Genetics Department of Physiology and Cell Biology Heart and Lung Research Institute Molecular Cellular and Developmental Biology (MCDB) Graduate Program Success in Graduate Education (SiGuE) Program The Ohio State University Columbus OH 43210 USA Department of Biological Sciences University of Toledo Toledo OH 43560 USA

The translation of the genotype into phenotype, represented for example by the expression of genes encod- ing enzymes required for the biosynthesis of phytochemicals that are important for interaction of plants with the environment, is largely carried out by transcription factors （TFs） that recognize specific cis-regulatory elements in the genes that they control. TFs and their target genes are organized in gene regulatory net- works （GRNs）, and thus uncovering GRN architecture presents an important biological challenge necessary to explain gene regulation. Linking TFs to the genes they control, central to understanding GRNs, can be car- ried out using gene- or TF-centered approaches. In this study, we employed a gene-centered approach uti- lizing the yeast one-hybrid assay to generate a network of protein-DNA interactions that participate in the transcriptional control of genes involved in the biosynthesis of maize phenolic compounds including gen- eral phenylpropanoids, lignins, and flavonoids. We identified 1100 protein-DNA interactions involving 54 phenolic gene promoters and 568 TFs. A set of 11 TFs recognized 10 or more promoters, suggesting a role in coordinating pathway gene expression. The integration of the gene-centered network with informa- tion derived from TF-centered approaches provides a foundation for a phenolics GRN characterized by in- terlaced feed-forward loops that link developmental regulators with biosynthetic genes.

关键词： phenylpropanoid flavonoid yeast one-hybrid chromatin immunoprecipitation

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Online Demand Response Characterization Based on Variability in Customer Behavior

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Journal of Modern Power Systems and Clean Energy 2024年第3期12卷 936-946页

作者： Lester Marrero daniel Sbárbaro luis García-Santander the Department of Electrical Engineering University of ConcepciónConcepción 4070409Chile

This paper proposes an online framework to characterize demand response(DR)over *** proposed framework facilitates obtaining and updating the daily consumption patterns of *** essential concept of response profile class(RPC)is introduced for characterization and complemented by the measure of the variability in customer *** paper uses a modified version of the incremental clustering by fast search and find of density peaks(CFSFDP)algorithm for daily profiles,considering the multivariate normal kernel density estimator and incremental forms of the Davies-Bouldin(i DB)and Xie-Beni(i XB)validity *** studies conducted using real-world and simulated daily profiles of residential and commercial Chilean end-users have demonstrated how the proposed framework can continuously characterize *** proposed framework is proven to achieve realistic customer models for effective energy management by estimating the customer response to price signals at the distribution system operator(DSO)level.

关键词： Demand response(DR) incremental indice online characterization online clustering response profile class(RPC)

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Association between Helicobacter pylori infection,mismatch repair,HER2 and tumor-infiltrating lymphocytes in gastric cancer

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2487-2503页

作者： Carlos A Castaneda Miluska Castillo luis A Bernabe Joselyn Sanchez Matteo Fassan Katherine Tello Ignacio Ivan Wistuba Ivan Chavez Passiuri Eloy Ruiz Juvenal Sanchez Fernando Barreda daniel Valdivia Yaqueline Bazan Milagros Abad-Licham Claudio Mengoa Hugo Fuentes Paola Montenegro Ebert Poquioma Raul Alatrista Claudio J Flores luis Taxa Faculty of Health Sciences Universidad Cientifica del SurLima 15038Peru GECO PERU Grupo de Estudios Clinicos Oncologicos del PeruLima 15038Peru Department of Research Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Faculty of Human Medicine Universidad Ricardo PalmaLima 15039Peru Department of Medicine Surgical Pathology&Cytopathology UnitUniversity of PaduaPadua 35121Italy Department of Translational Molecular Pathology The University of Texas MD Anderson Cancer CenterHoustonTX 77030United States Department of Abdominal Surgery Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Pathology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Medical Specialties Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Pathology Instituto Regional de Enfermedades Neoplasicas del NorteTrujillo 13001Peru Faculty of Human Medicine Universidad Privada Antenor OrregoTrujillo 13008Peru Department of Surgery Instituto Regional de Enfermedades Neoplasicas del SurArequipa 04002Peru Department of Medical Oncology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Epidemiology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Unidad de Investigación Básica y Traslacional Oncosalud-AUNALima 15038Peru Faculty of Medicine Universidad San Martin de PorresLima 15008Peru

BACKGROUND The influence of Helicobacter-pylori(***)infection and the characteristics of gastric cancer(GC)on tumor-infiltrating lymphocyte(TIL)levels has not been extensively *** of infiltrating-immune-cell subtypes as well as survival is necessary to obtain comprehensive *** To determine the rates of deficient mismatch-repair(dMMR),HER2-status and *** infection and their association with TIL levels in *** Samples from 503 resected GC tumors were included and TIL levels were evaluated following the international-TILs-working-group recommendations with assessment of the intratumoral(IT),stromal(ST)and invasive-border(IB)*** density of CD3,CD8 and CD163 immune cells,and dMMR and HER2-status were determined by immunohistochemistry(IHC).*** infection was evaluated by routine histology and quantitative PCR(qPCR)in a subset of *** dMMR was found in 34.4%,HER2+in 5%and ***-positive in 55.7%of *** IT-TIL was associated with grade-3(P=0.038),while ST-TIL with grade-1(P<0.001),intestinal-histology(P<0.001)and no-recurrence(P=0.003).dMMR was associated with high TIL levels in the ST(P=0.019)and IB(P=0.01)compartments,and STCD3(P=0.049)and ST-CD8(P=0.05)***2-was associated with high IT-CD8(P=0.009).*** was associated with high IT-TIL levels(P=0.009)when assessed by routine-histology,and with high TIL levels in the 3 compartments(P=0.002-0.047)and CD8 density in the IT and ST compartments(P=0.001)when assessed by qPCR.A longer overall survival was associated with low IT-CD163(P=0.003)and CD8/CD3(P=0.001 in IT and P=0.002 in ST)and high IT-CD3(P=0.021),ST-CD3(P=0.003)and CD3/CD163(P=0.002).CONCLUSION TIL levels were related to dMMR and *** CD8/CD3 and high CD163/CD3 were associated with lower recurrence and longer survival.

关键词： Lymphocytes Macrophages Gastric cancer Helicobacter pylori HER2 Mismatch repair

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RANKL inhibition reduces lesional cellularity and Gαs variant expression and enables osteogenic maturation in fibrous dysplasia

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Bone Research 2024年第1期12卷 165-179页

作者： luis F.de Castro Jarred M.Whitlock Zachary Michel Kristen Pan Jocelyn Taylor Vivian Szymczuk Brendan Boyce daniel Martin Vardit Kram Rebeca Galisteo Kamran Melikov Leonid V.Chernomordik Michael T.Collins Alison M.Boyce Skeletal Disorders and Mineral Homeostasis Section National Institute of Dental and Craniofacial ResearchNational Institutes of HealthBethesdaMDUSA Section on Membrane Biology Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaMDUSA Metabolic Bone Disorders Unit National Institute of Dental and Craniofacial ResearchNational Institutes of HealthBethesdaMDUSA Department of Plastic and Reconstructive Surgery The Johns Hopkins Medical InstitutionsBaltimoreMDUSA Department of Pathology and Laboratory Medicine University of Rochester Medical CenterRochesterNYUSA NIDCR Genomics and Computational Biology Core National Institute of Dental and Craniofacial ResearchNational Institutes of HealthBethesdaMDUSA

Fibrous dysplasia(FD)is a rare,disabling skeletal disease for which there are no established *** evidence supports inhibiting the osteoclastogenic factor receptor activator of nuclear kappa-B ligand(RANKL)as a potential treatment *** this study,we investigated the mechanisms underlying RANKL inhibition in FD tissue and its likely indirect effects on osteoprogenitors by evaluating human FD tissue pre-and post-treatment in a phase 2 clinical trial of denosumab(NCT03571191)and in murine in vivo and ex vivo preclinical *** analysis of human and mouse tissue demonstrated increased osteogenic maturation,reduced cellularity,and reduced expression of the pathogenic Gαs variant in FD lesions after RANKL *** sequencing of human and mouse tissue supported these *** interaction between osteoclasts and mutant osteoprogenitors was further assessed in an ex vivo lesion model,which indicated that the proliferation of abnormal FD osteoprogenitors was dependent on *** results from this study demonstrated that,in addition to its expected antiosteoclastic effect,denosumab reduces FD lesion activity by decreasing FD cell proliferation and increasing osteogenic maturation,leading to increased bone formation within *** findings highlight the unappreciated role of cellular crosstalk between osteoclasts and preosteoblasts/osteoblasts as a driver of FD pathology and demonstrate a novel mechanism of action of denosumab in the treatment of bone disease.

关键词： dysplasia treatment finding

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Unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neuropathology and behavioral deficits in parkinsonian rats withα-synucleinopathy

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Neural Regeneration Research 2024年第9期19卷 2057-2067页

作者： Bismark Gatica-Garcia Michael J.Bannon Irma Alicia Martínez-Dávila luis O.Soto-Rojas David Reyes-Corona Lourdes Escobedo Minerva Maldonado-Berny ME Gutierrez-Castillo Armando J.Espadas-Alvarez Manuel A.Fernandez-Parrilla Juan U.Mascotte-Cruz CP Rodríguez-Oviedo Irais E.Valenzuela-Arzeta Claudia Luna-Herrera Francisco E.Lopez-Salas Jaime Santoyo-Salazar daniel Martinez-Fong Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Laboratorio de Patogénesis Molecular Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Red de Medicina para la Educación y Desarrollo y la Investigación Científica de Iztacala(Red MEDICI) Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Nanoparticle Therapy Institute AguascalientesMéxico Departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Escuela Nacional de Medicina y Homeopatía Instituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Fisiología Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Biología Molecular y Biotecnología Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoMéxico Departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa de Nanociencias y Nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Parkinsonism by unilateral,intranigralβ-sitosterolβ-D-glucoside administration in rats is distinguished in that theα-synuclein insult begins unilaterally but spreads bilaterally and increases in severity over time,thus replicating several clinical features of Parkinson’s disease,a typicalα-*** Nurr1 repressesα-synuclein,we evaluated whether unilateral transfected of rNurr1-V5 transgene via neurotensin-polyplex to the substantia nigra on day 30 after unilateralβ-sitosterolβ-D-glucoside lesion could affect bilateral neuropathology and sensorimotor deficits on day 30 *** study found that rNurr1-V5 expression but not that of the green fluorescent protein(the negative control)reducedβ-sitosterolβ-D-glucoside-induced ***,a bilateral increase in tyrosine hydroxylase-positive cells and arborization occurred in the substantia nigra and increased tyrosine hydroxylase-positive ramifications in the *** addition,tyrosine hydroxylase-positive cells displayed less senescence markerβ-galactosidase and more neuron-cytoskeleton markerβIII-tubulin and brain-derived neurotrophic factor.A significant decrease in activated microglia(positive to ionized calcium-binding adaptor molecule 1)and neurotoxic astrocytes(positive to glial fibrillary acidic protein and complement component 3)and increased neurotrophic astrocytes(positive to glial fibrillary acidic protein and S100 calcium-binding protein A10)also occurred in the substantia *** effects followed the bilateral reduction inα-synuclein aggregates in the nigrostriatal system,improving sensorimotor *** results show that unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neurodegeneration(senescence and loss of neuron-cytoskeleton and tyrosine hydroxylase-positive cells),neuroinflammation(activated microglia,neurotoxic astrocytes),α-synuclein aggregation,and sensorimotor *** neurotrophic astrocytes and

关键词： A1 astrocytes A2 astrocytes gene therapy microglia motor deficits nanoparticles neurodegeneration neuroinflammation senescence α-synuclein aggregates

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Venopulmonary ECMO Improved Hypoxemia and Supported the Right Ventricle in a Patient with Decompensated Eisenmenger Syndrome

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World Journal of Cardiovascular Surgery 2024年第1期14卷 7-19页

作者： daniel Manzur-Sandoval Gian Manuel Jiménez-Rodríguez Edgar García-Cruz Ramón Espinosa-Soto Erika Yamali Ramirez-Marcano Yessenia Máyory Téllez-López José luis Elizalde-Silva Gustavo Rojas-Velasco Cardiovascular Critical Care Unit Ignacio Ch&#225vez National Institute of Cardiology Mexico City Mexico Adult Congenital Heart Disease Unit Ignacio Ch&#225vez National Institute of Cardiology Mexico City Mexico Cardiology Department Ignacio Ch&#225vez National Institute of Cardiology Mexico City Mexico

Mechanical circulatory and/or respiratory assistance with extracorporeal membrane oxygenation (ECMO) has become a standard of care for patients with circulatory (venoarterial) and/or respiratory (venovenous) failure refractory to standard therapies. Adult patients with congenital heart disease are an increasingly recognized and growing population and include various groups, such as undiagnosed cases in childhood and palliated and/or corrected cases, which require subsequent care because of residual lesions, cardiac arrest/insufficiency, and arrhythmias, among other conditions. In addition, these patients are prone to developing pathologies that are typical of adulthood with a generally increased risk of morbidity and mortality because of their low reserves and organic damage associated with the underlying heart disease, which makes them candidates for ECMO. These patients represent an additional challenge in this therapy because malformations and the presence of a shunt can generally affect the usual cannulation methods and hemodynamic and oximetry monitoring. Thus, the configuration decision must be made on a case-by-case basis. Here, we present a cannulation method, venopulmonary artery ECMO, which provides hemodynamic and respiratory support, and may be ideal for patients with shunts and/or right ventricular dysfunction. To our knowledge, this is the first report of this configuration in patients with congenital heart diseases.

关键词： Eisenmenger Syndrome Venopulmonary Artery ECMO Refractory Hypoxemia Right Ventricular Dysfunction

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Adding fifth dimension to optoacoustic imaging: volumetric time-resolved spectrally enriched tomography

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Light(Science & Applications) 2014年第1期3卷 330-334页

作者： Xose luis Dean-Ben daniel Razansky Institute for Biological and Medical Imaging(IBMI) Technical University of Munich and Helmholtz Center Munich85764 NeuherbergGermany

Optoacoustics provides a unique set of capabilities for bioimaging,associated with the intrinsic combination of ultrasound-and light-related advantages,such as high spatial and temporal resolution as well as powerful spectrally enriched imaging contrast in biological *** demonstrate here,for the first time,the acquisition,processing and visualization of five-dimensional optoacoustic data,thus offering unparallel imaging capacities among the current bioimaging *** newly discovered performance is enabled by simultaneous volumetric detection and processing of multispectral data and is further showcased here by attaining time-resolved volumetric blood oxygenation maps in deep human vessels and real-time tracking of contrast agent distribution in a murine model in vivo.

关键词： biophotonics multispectral optoacoustic imaging photoacoustic tomography

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Tribological performance of uncoated and TiCN-coated D2,M2 and M4 steels under lubricated condition

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Journal of Iron and Steel Research International 2017年第8期24卷 823-829页

作者： luis daniel Aguilera Camacho Santos García Miranda Karla Judith Moreno Instituto Tecnologico de Celaya/TNM Apartado Postal 5738010-CelayaGuanajuatoMexico

Hard coatings are used to improve the wear resistance of metals which largely depends on adhesion between substrate and *** wear and friction behavior of uncoated and TiCN-coated D2,M2 and M4 steels were evaluated by apin-on-disk test under lubricated *** order to evaluate the influence of lubricant on wear performance,dry friction tests were also *** results showed that friction coefficients were very similar for both uncoated and TiCN-coated *** lubricated conditions,the uncoated D2 tool steel exhibited the lowest friction coefficient,but the TiCN-coated D2 steel presented the smallest wear *** was the main wear mechanism in all the ***,microhardness measurements were carried out,finding an influence of the steel substrate on the hardness of the ***,adhesion test was conducted,suggesting agood adhesion of class 1 between substrates and TiCN coatings.

关键词： D2 tool steel M2 high speed steel M4 high speed steel TiCN coating Friction Wear

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Prognostic role of selection criteria for liver transplantation in patients with hepatocellular carcinoma:Review and bibliometric

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iLIVER 2024年第1期3卷 52-61页

作者： Pamela Scarlett Espinoza Loyola Diana Laura Muratalla Bautista Karen Adela Hernández Bautista Elizabeth Gil White JoséAntonio González Moreno daniel Angel Torres del Real Víctor Manuel Páez Zayas Carla Escorza-Molina Fernando Mondragón Rodríguez Omar Vásquez Gómez luis Jorge Fernández López Paul Santiago Mogrovejo Vázquez Isidoro Aczel Sánchez-Cedillo Víctor Jose Visag Castillo Social Service Internship Universidad Nacional Autónoma de MéxicoMexico City 04510Mexico Social Service Internship Universidad AnahuacMexico City 04510Mexico Department of Surgery Division of Transplantation Surgery Hospital General de MéxicoMexico City 06720Mexico Departament of Gastroenterology Division of HepatologyHospital General de MéxicoMexico City 06720Mexico Departmanet of Anesthesiology Hospital General de MéxicoMexico City 06720Mexico

Hepatocellular carcinoma(HCC)is one of the most common cancers in the world and is one of the leading indications for liver transplantation,liver transplantation is the gold standard treatment for end stage liver *** is based up on radiological characteristics and rarely biopsy *** treatment must be individualized to each patient to improve recurrences and *** this article,we focus on the different selection criteria for liver *** study aimed to investigate the distribution laws and research frontiers of international literature,so as to present holistic bibliometric evaluation of the studies on 5-year survival and disease-free recurrence in 5 years,according to hepatocarcinoma criteria for liver *** paper aims to review and analyze 5-year survival and disease-free recurrence based on hepatocarcinoma criteria for liver *** systematically examines and summarizes distribution characteristics and research frontiers through bibliometric analysis.A bibliographic search was implemented in PubMed/Medline,Clinical Key,Science Direct and Index Medicus with MESH terms,from the year 1996–*** selected for transplantation using the Metroticket 2.0(MT2)criteria had the highest overall survival along with patients selected for transplantation using the Milan Criteria had the best 5-year disease-free *** Metroticket 2.0 criteria(MT2)and Milan Criteria(MC)have shown the most favorable post-transplant outcomes for patients with hepatocellular carcinoma(HCC).However,MC demonstrated the best 5-year disease-free recurrence rate,underscoring the significance of taking into account tumor morphology and biology when determining the eligibility of HCC patients for liver *** distribution characteristics and research frontiers by bibliometrics concerning prognostic role of selection criteria for liver transplantation in patients with hepatocellular carcinoma the collaboration

关键词： Hepatocellular carcinoma Liver transplantation Survival Criteria Recurrence Bibliometrics

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Minimally invasive nephrectomy for inflammatory renal disease

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Asian Journal of Urology 2020年第4期7卷 345-350页

作者： Paula Andrea Pena Lynda Torres-Castellanos German Patino Stefania prada luis Gabriel Villarraga Nicolas Fernandez Pontificia Universidad Javeriana School of MedicineBogota D.C.Colombia Department of Urology Hospital Universitario San IgnacioPontificia Universidad JaverianaSchool of MedicineBogota D.C.Colombia Division of Urology Hospital Universitario San IgnacioPontificia Universidad JaverianaBogota D.C.Colombia Division of Urology Hospital Universitario San IgnacioPontificia Universidad JaverianaSchool of MedicineBogota D.C.Colombia Department of Urology Fundacion Santa Fe de BogotaColombia Division of Urology Hospital for SickKidsUniversity of TorontoTorontoCanada

Objective:Once chronic inflammatory renal disease(IRD)develops,it creates a severe peri-fibrotic process,which makes it a relative contraindication for minimally invasive surgery(MIS).Our objective is to show that laparoscopic nephrectomy(LN)is a surgical option in IRD with fewer complications and better ***:Retrospective review of patients who underwent a modified-surgical laparoscopic transperitoneal nephrectomy was *** search included all operated patients between May 2013 and May 2018 that had a pathology result with any renal inflammatory condition(xanthogranulomatous pyelonephritis,chronic nephritis,and renal tuberculosis).We describe intra-operative variables such as operative time,blood loss,conversion rate,postoperative complications and length of hospital ***:There were 51 patients who underwent laparoscopic nephrectomy with a confirmatory pathology report for *** identified four(8%)major complications;three of them required transfusion and one conversion to open *** mean operative time was 233108 *** estimated blood loss was 206242 mL excluding the conversion cases and 281423 mL including *** mean length of hospital stay was 3.02.0 ***:Laparoscopic nephrectomy for IRD can safely be *** is a reproducible technique with low risks and complication *** experience supports that releasing the kidney first and leaving the hilum for the end is a safe approach when vascular structures are embedded into a single block of inflammatory and scar tissue.

关键词： Inflammation Kidney diseases Laparoscopy Minimally invasive surgical procedures Nephrectomy Nephritis Nephroureterectomy

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