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Reliable cell purification and determination of cell purity:crucial aspects of olfactory ensheathing cell transplantation for spinal cord repair

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Neural Regeneration Research 2020年第11期15卷 2016-2026页

作者： Ronak Reshamwala Megha Shah lucy belt Jenny A.K.Ekberg James A.St John Griffith Institute for Drug Discovery Griffith UniversityBrisbaneQLDAustralia Menzies Health Institute Queensland Griffith UniversitySouthportQLDAustralia Clem Jones Centre for Neurobiology and Stem Cell Research Griffith UniversityBrisbaneQLDAustralia

Transplantation of olfactory ensheathing cells,the glia of the primary olfactory nervous system,has been trialed for spinal cord injury repair with promising but variable outcomes in animals and *** ensheathing cells can be harvested either from the lamina propria beneath the neuroepithelium in the nasal cavity,or from the olfactory bulb in the *** these areas contain several other cell types,isolating and purifying olfactory ensheathing cells is a critical part of the *** is largely unknown how contaminating cells such as fibroblasts,other glial cell types and supporting cells affect olfactory ensheathing cell function post-transplantation;these cells may also cause unwanted *** is also,however,possible that the presence of some of the contaminant cells can improve ***,we reviewed the last decade of olfactory ensheathing cell transplantation studies in rodents,with a focus on olfactory ensheathing cell *** analyzed how purification methods and resultant cell purity differed between olfactory mucosa-and olfactory bulb-derived cell *** analyzed how the studies reported on olfactory ensheathing cell purity and which criteria were used to define cells as olfactory ensheathing ***,we analyzed the correlation between cell purity and transplantation *** found that olfactory bulb-derived olfactory ensheathing cell preparations are typically purer than mucosa-derived *** concluded that there is an association between high olfactory ensheathing cell purity and favourable outcomes,but the lack of olfactory ensheathing cell-specific markers severely hampers the field.

关键词： antibody astrocyte fibroblast glia glial cell injury nerve neuron trauma surgery

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The Impact of One Road One belt Initiative on East African Infrastructure

The Impact of One Road One Belt Initiative on East African I...

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作者： lucy PATRICK MUGOBE 浙江工商大学

学位级别：硕士

The main purpose of this study is to explore the impact of China’s One belt One Road initiative on the countries in East *** study sought to assess the gains this policy has made on the political,economic,infrastructural development of East *** was extensively reviewed and a theory such as gravity theory was reviewed to underline the framework of this *** study was basically a descriptive study reviewing various studies to draw a *** was gathered thoroughly through published articles and reports from different *** study found out that the OBOR initiative has so far had a significant impact on eastern African countries such as Kenya,Burundi,Tanzania,Uganda,and *** impacts were evident in mostly the infrastructural development of these *** study recommends that similar study be carried out in other sub regions of the continent so as to have a wider view of the impact of the OBOR initiative.

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