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Refractory Black Carbon Results and a Method Comparison between Solid-state Cutting and Continuous Melting Sampling of a West Antarctic Snow and Firn Core

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Advances in Atmospheric Sciences 2020年第5期37卷 545-554页

作者： luciano marquetto Susan KASPARI Jefferson Cardia SIMOES Emil BABIK Department of Geological Sciences Central Washington UniversityEllensburgWashington 98926USA Polar and Climatic Center Federal University of Rio Grande do SulPorto AlegreRio Grande do Sul91509-900Brazil

This work presents the refractory black carbon(rBC)results of a snow and firn core drilled in West Antarctica(79°55'34.6"S,94°21'13.3"W)during the 2014?15 austral summer,collected by Brazilian researchers as part of the First Brazilian West Antarctic Ice Sheet *** core was drilled to a depth of 20 m,and we present the results of the first 8 m by comparing two subsampling methods—solid-state cutting and continuous melting—both with discrete *** core was analyzed at the Department of Geological Sciences,Central Washington University(CWU),WA,USA,using a single particle soot photometer(SP2)coupled to a CETAC Marin-5 *** continuous melting system was recently assembled at CWU and these are its first *** also present experimental results regarding SP2 reproducibility,indicating that sample concentration has a greater influence than the analysis time on the reproducibility for low rBC concentrations,like those found in the Antarctic *** was carried out using mainly the rBC variation and sulfur,sodium and strontium as secondary parameters,giving the core 17 years(1998?2014).The data show a well-defined seasonality of rBC concentrations for these first meters,with geometric mean summer/fall concentrations of 0.016μg L^?1 and geometric mean winter/spring concentrations of 0.063μg L^?*** annual rBC concentration geometric mean was 0.029μg L^?1(the lowest of all rBC cores in Antarctica referenced in this work),while the annual rBC flux was 6.1μg m^?2 yr^?1(the lowest flux in West Antarctica records so far).

关键词： black carbon West Antarctica ice core single particle soot photometer

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A simplified pneumatic model for air brake of passenger trains

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Railway Engineering Science 2023年第2期31卷 145-152页

作者： luciano Cantone Andrea Ottati Department of Enterprise Engineering“Mario Lucertini” University of Rome Tor VergataRomeItaly Technical Direction TrenitaliaFSI GroupFlorenceItaly

Braking system performance is relevant for both railway safety and network optimization. Most trains employ air brake systems;air brake systems of freight trains mostly cannot achieve a synchronous application of brake forces, which is usually customary for passenger trains. The paper generalizes a previous air brake pneumatic model to passenger trains and describes the needed modifications. Among them, the way the pressure reduces in the brake pipe is generalized. Moreover, this paper reports an analytical bi-dimensional function for calculating the nozzle diameter equivalent to the electro-pneumatic(EP) or the electronically controlled pneumatic(ECP)brake valve as a function of the wagon length and the time to vent the brake pipe locally. The numerical results of the new model are compared against several experimental tests of high-speed passenger trains of Trenitalia, namely ETR500 and ETR1000. The model is suitable to be integrated into the UIC software TrainDy, aiming to extend its computational field to passenger trains and to simulate the safety of trains during a recovery.

关键词： EP or ECP modeling Model validation TrainDy Train brake Air brake

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On conservation issues of contemporary architecture： The technical design development and the ageing process of the Jubilee Church in Rome by Richard Meier

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Frontiers of Architectural Research 2018年第2期7卷 107-121页

作者： luciano Cardellicchio Kent School of Architecture University of Kent Marlowe Building Canterbury Kent CT2 7NR United Kingdom

A vast amount of iconic buildings distinguished by complex geometries have been constructed in the last two decades in the United Kingdom, Germany, Spain, Portugal, France and Italy. Overall, the construction of these iconic buildings has led to technical innovations. As these buildings are often erected following customised construction details and bespoke technical solutions that are rarely tested in advance, measuring their ageing process has become crucial to understand if these geometries are sustainable in terms of the cost of their maintenance. This study aims to analyse the technical design development and the ageing process of the Jubilee Church in Rome by Richard Meier. Only fourteen years after the opening, this building is affected by extensive decay of construction materials due to both wrong technical design choices and lack of unaffordable maintenance work. This study aims to identify the causes of the premature decay of this building, recording retroactively its technical development and mapping the current state of damages.

关键词： Meier Jubitee ChurchDecay Technology Contemporary Conservation

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Multiphoton single-molecule localization by sequential excitation with light minima

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Light(Science & Applications) 2022年第4期11卷 462-465页

作者： luciano A.Masullo Fernando D.Stefani Centro de Investigaciones en Bionanociencias(CIBION) Consejo Nacional de Investigaciones Cientifcas y Tecnicas(CONICETGodoy Cruz 2390C1425FQD Ciudad Autonoma de Buenos AiresBuenos AiresArgentina Departamento de Fisica Facultad de Ciencias Exactas y NaturalesUniversidad de Buenos AiresGuiraldes 2620C1428EHA Ciudad Autonoma de Buenos AiresBuenos AiresArgentina Present address:Max Planck Institute of Biochemistry Am Klopferspitz 1882152 MartinsriedGermany

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1-2 nm precision with moderate photon counts,enabling tracking and superresolution imaging with true molecular *** this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.

关键词： excitation photon light

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Genes for RNA-binding proteins involved in neuralspecific functions and diseases are downregulated in Rubinstein-Taybi iNeurons

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Neural Regeneration Research 2022年第1期17卷 5-14页

作者： Lidia Larizza luciano Calzari Valentina Alari Silvia Russo Cytogenetics and Molecular Genetics Laboratory IRCCS Istituto Auxologico ItalianoMilanoItaly

Taking advantage of the fast-growing knowledge of RNA-binding proteins(RBPs)we review the signature of downregulated genes for RBPs in the transcriptome of induced pluripotent stem cell neurons(iNeurons)modelling the neurodevelopmental Rubinstein Taybi Syndrome(RSTS)caused by mutations in the genes encoding CBP/p300 *** discuss top and functionally connected downregulated genes sorted to“RNA processing”and“Ribonucleoprotein complex biogenesis”Gene Ontology *** first set of downregulated RBPs includes members of hnRNHP(A1,A2B1,D,G,H2-H1,MAGOHB,PAPBC),core subunits of U small nuclear ribonucleoproteins and Serine-Arginine splicing regulators families,acting in precursor messenger RNA alternative splicing and *** with literature findings on reduced transcript levels of serine/arginine repetitive matrix 4(SRRM4)protein,the main regulator of the neural-specific microexons splicing program upon depletion of Ep300 and Crebbp in mouse neurons,RSTS iNeurons show downregulated genes for proteins impacting this *** link downregulated genes to neurological disorders including the new HNRNPH1-related intellectual disability syndrome with clinical overlap to *** set of downregulated genes for Ribosome biogenesis includes several components of ribosomal subunits and nucleolar proteins,such NOP58 and fibrillarin that form complexes with snoRNAs with a central role in guiding post-transcriptional modifications needed for rRNA *** nucleolar proteins are“dual”players as fibrillarin is also required for epigenetic regulation of ribosomal genes and conversely NOP58-associated snoRNA levels are under the control of NOP58 interactor BMAL1,a transcriptional regulator of the circadian *** downregulated genes for“dual specificity”RBPs such as RUVBL1 and METTL1 highlight the links between chromatin and the RBP-ome and the contribution of perturbations in their cross-talk to *** underline the hub position o

关键词： alternative splicing CBP/p300 chromatin regulators downregulated genes induced pluripotent stem cell-neurons neurodevelopmental disorders ribosome biogenesis RNA-binding proteins RNASeq Rubinstein-Taybi

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Towards a Formal Semantics for UML/MARTE State Machines Based on Hierarchical Timed Automata

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Journal of Computer Science & Technology 2013年第1期28卷 188-202页

作者： Yu Zhou luciano Baresi Matteo Rossi College of Computer Science Nanjing University of Aeronautics and Astronautics Nanjing 210016 China State Key Laboratory for Novel Software Technology Nanjing University Nanjing 210093 China Department of Electronics and Information Politecnico di Milano Milan 20133 Italy

UML is a widely-used, general purpose modeling language. But its lack of a rigorous semantics forbids the thorough analysis of designed solution, and thus precludes the discovery of significant problems at design time. To bridge the gap, the paper investigates the underlying semantics of UML state machine diagrams, along with the time-related modeling elements of MARTE, the profile for modeling and analysis of real-time embedded systems, and proposes a formal operational semantics based on extended hierarchical timed automata. The approach is exemplified on a simple example taken from the automotive domain. Verification is accomplished by translating designed models into the input language of the UPPAAL model checker.

关键词： timed automata state machine diagram formal semantics

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Quantifying the relative importance of direct and indirect effects influencing bird nestling growth

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Integrative Zoology 2022年第3期17卷 408-419页

作者： luciano N.SEGURA Facundo X.PALACIO División Zoología Vertebrados Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataBuenos AiresArgentina

Nestling growth parameters are integral components of avian life-history strategies as they are crucial determinants of individual *** many factors impact on nestling growth,the relative contribution of each one is still debated in the *** studies rely on the assumption that each factor directly affects nestling growth,but indirect effects mediated by other factors are usually the rule in *** this study,we present a comprehensive view of both direct and indirect factors affecting nestling growth using the Red-crested Cardinal(Paroaria coronata)as model *** evaluated the relative importance of different habitat(forest structure),biotic interactions(botfly larvae ectoparasitism,number of siblings,hatching order),and temporal factors(time of breeding)on nestling growth parameters in 278 nestlings of 128 nests by using piecewise structural equation *** found that botfly ectoparasitism had the strongest direct effect on nestling growth and,in turn,forest structure increased the probability of botfly ***,the interaction between the number of siblings and hatching order influenced nestling growth,indicating that the first and second nestlings had disproportionately higher growth rates in large than in small *** of breeding also showed a strong positive indirect effect on botfly occurrence,as well as a weak direct positive effect on nestling *** results demonstrate that,under natural conditions,nestling growth is driven by different factors acting not only directly,but also indirectly on this essential life history trait,and that these factors weave a complex web of interrelated variables.

关键词： biotic interactions forest structure Red-crested Cardinal structural equation models time of breeding

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β-Estradiol 17-acetate enhances the in vitro vitality of endothelial cells isolated from the brain of patients subjected to neurosurgery

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Neural Regeneration Research 2023年第2期18卷 389-395页

作者： Sonia Guzzo Pasquale De Bonis Barbara Pavan luciano Fadiga Department of Neuroscience and Rehabilitation Section of PhysiologyUniversity of FerraraFerraraItaly Center for Translational Neurophysiology of Speech and Communication(CTNSC) Italian Institute of Technology(IIT)FerraraItaly Department of Translational Medicine and for Romagna University of FerraraFerraraItaly

In the current landscape of endothelial cell isolation for building in vitro models of the blood-brain barrier,our work moves towards reproducing the features of the neurovascular unit to achieve glial compliance through an innovative biomimetic coating technology for brain chronic *** hypothesized that the autologous origin of human brain mic rovascular endothelial cells(hBMECs)is the first requirement for the suitable coating to prevent the glial inflammato ry response trigge red by foreign *** re,this study established a new procedure to preserve the in vitro viability of hBMECs isolated from gray and white matter specimens taken from neurosurge ry *** adult hBMECs is generally considered a challenging task due to the difficult survival ex vivo and progressive reduction in proliferation of these *** addition of 10 nMβ-estradiol 17-acetate to the hBMEC culture medium was found to be an essential and discriminating factor promoting adhesion and proliferation both after isolation and thawing,suppo rting the well-known protective role played by estrogens on *** particular,β-estradiol 17-acetate was critical for both freshly isolated and thawed female-derived hBMECs,while it was not necessary for freshly isolated male-derived hBMECs;however,it did countera ct the decay in the viability of the latter after *** tumo r-free hBMECs were thus cultured for up to 2 months and their growth efficiency was assessed befo re and after two periods of *** pite the thermal stress,the hBMECs remained viable and suitable for re-freezing and storage for several *** approach increasing in vitro viability of hBMECs opens new perspectives for the use of cryopreserved autologous hBMECs as biomimetic therapeutic tools,offering the potential to avoid additional surgical sampling for each patient.

关键词： β-estradiol 17-acetate 17β-estradiol cryopreservation gender-specific gray matter human brain microvascular endothelial cells surgical resections vascular protection white matter

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Advances in selenium supplementation:From selenium-enriched yeast to potential selenium-enriched insects,and selenium nanoparticles

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Animal Nutrition 2023年第3期14卷 193-203页

作者： Luca Ferrari Donata M.I.R.Cattaneo Rossella Abbate Michele Manoni Matteo Ottoboni Alice luciano Christoph von Holst luciano Pinotti Department of Veterinary Medicine and Animal Sciences(DIVAS) Universita degli Studi di Milano26900 LodiItaly European Commission Joint Research Centre(JRC)2440 GeelBelgium CRC I-WE(Coordinating Research Centre:Innovation for Well-Being and Environment) Universita degli Studi di Milano20133 MilanItaly

Selenium(Se)is an essential micronutrient that plays an important role in animal and human development and physiological *** review surveys the role of Se in the environment,plants and animal bodies,and discusses data on Se biofortification with different sources of supplementation,from inorganic to organic forms,with special focus on Se-enriched yeast(Se-yeast).Although Se-yeast remains one of the main sources of organic Se,other emerging and innovative sources are reviewed,such as Se-enriched insects and Se-nanoparticles and their potential use in animal ***-enriched insects are discussed as an option for supplying Se in organic form to livestock ***-nanoparticles are also discussed,as they represent a more biocompatible and less toxic source of inorganic Se for animal organisms,compared to selenite and *** also provide up to date information on the legal framework in the EU,USA,and Canada of Se that is contained in feed *** the scientific evidence available in the literature,it can be concluded that among the inorganic forms,sodium selenite is still one of the main options,whereas Se-yeast remains the primary organic ***,other potential sources such as Se-enriched insects and Se-nanoparticles are being investigated as they could potentially combine a high bioavailability and reduced Se emissions in the environment.

关键词： Selenium Selenium-enriched yeast Selenium nanoparticle Selenium-enriched insect Speciation analysis

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Different canopy openings affect underground traits in herbaceous plants of a southern forest in Patagonia

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Journal of Plant Ecology 2016年第5期9卷 542-552页

作者： luciano J.Selzer Carlos A.Busso CADIC-CONICET Houssay 200(9410)UshuaiaTierra del FuegoArgentina.Instituto de Ciencias PolaresAmbiente y Recursos Naturales.Universidad Nacional de Tierra del FuegoAlem 1036(9410)UshuaiaTierra del FuegoArgentina CERZOS-CONICET San Andrés 800(8000)Bahía BlancaBuenos AiresArgentina.Depto.de Agronomía-Universidad Nacional del SurSan Andrés 800(8000)Bahía BlancaBuenos AiresArgentina

Aims Forest canopy openings modify the natural environment,producing changes in light quality and intensity,precipitation and *** turn,these changes promote the acclimation of understory ***,little work has been done on underground responses to those environmental *** objective of this work was to determine how Osmorhiza depauperata,Phleum alpinum and Poa pratensis change its root length density and root colonization by mycorrhiza as a function of light availability in a Nothofagus pumilio(***)forest harvested following the variable retention *** We selected three microenvironments in an old growth forest harvested by the variable retention prescription:aggregated retention,dispersed retention with influence of aggregated retention and dispersed retention.A non-harvested primary forest(PF),similar to the harvested one,was used as a *** 2 months,from October 2008 to April 2009,we took soil cores from randomly selected *** these soil cores,root length density and colonization percentage(CP)by arbuscular mycorrhizae were *** Findings Light availability changed significantly among the *** general,root length density was significantly greater in *** than in *** and both species greater than in *** availability increased root length density in all species,although the magnitude of these increases difference among *** length density was 187%greater in ***,101%in *** and 94%in *** in the disperse retention system than in the *** CP was higher in *** than in *** and ***,it was lower in the PF than in the harvested *** were very low.

关键词： root length density mycorrhizal colonization light shade understory Tierra del Fuego

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