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Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp Litopenaeus vannamei(Crustacea,Decapoda)

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Advances in Bioscience and Biotechnology 2014年第3期5卷 273-281页

作者： Michele Mantovani Goncalves luciana Correia de almeida Regitano Cosme Damiao Cruz Caio Cesio Salgado Patricia Domingues de Freitas Joao Luis rocha Ana Karina Guerrelhas Teixeira Pedro Manoel Galetti-Junior Departamento de Genetica e Evolucao Universidade Federal de Sao CarlosSao CarlosBrasil Embrapa Pecuaria Sudeste Sao CarlosSPBrasil Departamento de Biologia Geral Universidade Federal de VicosaVicosaBrasil Genearch Aquacultura Ltda Rua Pedro Zuca s/nPraia de PititingaRio do FogoRNBrasil

The cultivation of the marine species of shrimp Litopenaeus vannamei has emerged as one of the segments that best develop in the Brazilian aquaculture, representing the agribusiness that grew the most in recent years. Regarding the economic importance of the farming of this species in Brazil, further studies on genetic improvement were conducted. A F2 segregating population consisting of 192 samples for each G1 and G2 families, from crossing inbred lines was used for the studies performed in the present work. The genetic linkage analysis was based on polymorphic markers derived from nine AFLP (Amplified Fragment Length Polymorphism) primers. Fourteen genetic linkage groups including 103 segregating polymorphic markers were constructed covering350 cMfor G1 and four genetic linkage groups including 59 markers were constructed covering300 cMfor G2. Simple marker analyses were performed among individuals evaluated phenotypically, finding markers linked to genes that may be potentially important and useful to assess characteristics of economic importance for traits related to weight and the disease IMN (idiopathic muscle necrosis). The statistical model including markers explained major proportion of phenotypic characteristic weight in relation to disease incidence IMN.

关键词： Shrimp Litopenaeus vannamei AFLP Genetic Linkage Groups

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Green Tea(Camellia sinensis):Hypocholesterolemic Effects in Humans and Anti-Inflammatory Effects in Animals

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Food and Nutrition Sciences 2014年第22期5卷 2185-2194页

作者： Márcia Reto Cristina almeida Joao rocha Bruno Sepodes Maria-Eduardo Figueira Research Institute for Medicines(iMed.ULisboa) Faculty of PharmacyUniversity of LisbonLisbonPortugal

In its essence, tea is an infusion of Camellia sinensis leaves and it is the most widely consumed beverage in the world, aside water. Animal and epidemiological studies have associated green tea consumption with several health benefits, which include hypocholesterolemic effect and anti-in-flammatory activity. In this study catechins levels of green tea and its effect on the lipid profile of humans were evaluated as well as the protective effects against H2O2-mediated damage in human fibroblasts and anti-inflammatory activity of (–)-epigallocatechingallate (EGCG). The daily consumption of 1200 mL of green tea for 30 days by 15 human volunteers promoted the decrease of cholesterol and LDL levels after 15 days, but this effect was not persistent after 30 days. No changes were observed in lipid profile after the consumption of green tea capsules. EGCG, a major green tea catechin, demonstrated an anti-inflammatory action in rats and an antioxidant effect in cultured human fibroblasts.

关键词： Green Tea Infusions Hypocholesterolemic Effect Anti-Inflammatory Activity Antioxidant Activity

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Rheological and stability analysis of cement pastes incorporating silica-based wastes

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Particuology 2024年第6期89卷 144-152页

作者： Julia da Rosa Martins Janaíde Cavalcante rocha Dachamir Hotza Luciano Senff Graduate Program in Materials Science and Engineering(PPGMAT) Federal University of Santa Catarina(UFSC)Florianópolis88040-900SCBrazil Graduate Program in Civil Engineering(PPGEC) Federal University of Santa Catarina(UFSC)Florianópolis88040-900SCBrazil Graduate Program in Chemical Engineering(POSENQ) Federal University of Santa Catarina(UFSC)Florianópolis88040-900SCBrazil Department of Mobility Engineering Federal University of Santa Catarina(UFSC)Joinville89219-600SCBrazil

This study investigates the influence of waste characteristics,especially zeta potential,on the properties of cement pastes and *** focus is to evaluate the impact of the zeta potential of cement particles and waste materials on the sedimentation speed,rheology,and hardening time of stabilized cement *** Cement II F 40,retarder additive,silica,and fly ash were used in the *** pastes were prepared,and during the stabilization period,their rheological properties and pH were *** zeta potential and sedimentation speed of the cement and waste particles were measured at the pH that the pastes presented during the entire stabilization *** the stabilization period,the pastes were subjected to the hardening time *** zeta potential analyses revealed diverse values for the different powder types,with the cement particles exhibiting a zeta potential of−3.0 mV,the silica particles exhibiting−10.5 mV,and the fly ash particles exhibiting−20.3 *** influence of the high zeta potential modulus was observed on the sedimentation speed,with the solution containing fly ash exhibiting a speed of 40.01μm/s,whereas the solution containing only cement exhibited a speed of 99.38μm/*** the pastes,the results indicate that the presence of fly ash particles with a significantly negative zeta potential led to a 16%reduction in hardening time compared to particles with a lower modulus of zeta *** tests showed that the inclusion of fly ash particles prevented the formation of *** the zeta potential influenced agglomerate formation and hardening time,it was found to have no effect on yield stress or viscosity.

关键词： Wastes Zeta potential Setting time Rheometry

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Scleral surgery for the treatment of presbyopia: where are we today?

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Eye and Vision 2018年第1期5卷 37-47页

作者： AnnMarie Hipsley Brad Hall Karolinne M.rocha Ace Vision Group Inc 39655 Eureka DriveNewarkCA 94560USA Sengi Data CambridgeONCanada Storm Eye Institute Medical University of South CarolinaCharlestonSCUSA

Presbyopia corrections traditionally have been approached with attempts to exchange power,either at the cornea or the lens planes,inducing multifocality,or altering asphericity to impact the optical *** that affect the visual axis,such as spectacle and contact lens correction,refractive surgeries,corneal onlays and inlays,and intraocular lenses are typically unable to restore true accommodation to the presbyopic *** aim is instead to enhance‘pseudoaccommodation’by facilitating an extended depth-of-focus for which vision is *** is a true lack of technology that approaches presbyopia from a treatment based or therapy based solution,rather than a‘vision correction’solution that compromises other components of the optical *** surgical procedures seek to restore true accommodation combined with pseudoaccommodation and have several advantages over other more invasive options to treat *** the theoretical justification of scleral surgical procedures remains controversial,there has nevertheless been increasing interest and evidence to support scleral surgical and therapeutic approaches to treat *** progress in scleral surgery techniques and understanding of the mechanisms of action have been achieved since the 1970s,and this remains an active area of *** this article,we discuss the historic scleral surgical procedures,the two scleral procedures currently available,as well as an outlook of the future for the scleral surgical space for treating presbyopia.

关键词： Scleral surgery Presbyopia Accommodation Presbyopia treatment

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Water yield and biomass production for on a eucalypt-dominated Mediterranean catchment under different climate scenarios

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Journal of Forestry Research 2023年第5期34卷 1263-1278页

作者： Joao rocha Ana Quintela Dalila Serpa Jan Jacob Keizer Sérgio Fabres RAIZ-Forest and Paper Research Institute Quinta de S.FranciscoRua JoséEstevao(EN 230-1)3800–783 EixoAveiroPortugal Department of Environment and Planning Centre of Environmental and Marine Studies(CESAM)University of AveiroCampus Universitário de Santiago3810–193 AveiroPortugal

Worldwide,forests are vital in the regulation of the water cycle regulation and in water balance *** of ecohydrological responses of production forests is essential to support management strategies,especially where water is already *** climatological patterns are expected to impact thermopluviometric regimes,water cycle components,hydrological responses,and plant physiology,evapotranspiration rates,crop productivity and land management *** work(1)assessed the impacts of different predicted climate conditions on water yield;(2)inferred the impacts of climate change on biomass production on eucalypt-to-eucalypt succes *** this end,the widely accepted Soil and Water Assessment Tool(SWAT)was run with the RCA,HIRHAM5 and RACMO climate models for two emission scenarios(RCP 4.5 and8.5).Three 12-year periods were considered to simulate tree growth under coppice *** results revealed an overall reduction in streamflow and water yield in the catchment in line with the projected reduction in total annual ***,HIRHAM5 and RACMO models forecast a slight shift in seasonal streamflow of up to 2 months(for2024-2048)in line with the projected increase in precipitation from May to *** biomass production,the extreme climate model(RCA)and severe emis sion scenario(RCP 8.5)predicted a decrease up to 46%.However,in the less extreme and more-correlated(with actual catchment climate conditions)climate models(RACMO and HIRHAM5)and in the less extreme emission scenario(RCP 4.5),biomass production increased(up to 20%),and the growth cycle was slightly *** was proven to be a valuable tool to assess climate change impacts on a eucalypt-dominated catchment and is a suitable decision-support tool for forest managers.

关键词： Climate scenarios Forested catchments Forest ecohydrological modelling Eucalypt biomass production SWAT model

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Denitrification-nitrification process in permeable coastal sediments:An investigation on the effect of salinity and nitrate availability using flow-through reactors

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Acta Oceanologica Sinica 2021年第9期40卷 1-12页

作者： Shan Jiang Mark Kavanagh Juan Severino Pino Ibanhez Carlos rocha State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200062China School of Natural Sciences Trinity College DublinDublin 2Ireland Trinity Centre for the Environment School of Natural SciencesTrinity College DublinDublin 2Ireland Instituto de Investigacions Marinas Consejo Superior de Investigaciones CientiTicasVigo 36208Spain

Permeable coastal sediments act as a reactive node in the littoral zone,transforming nutrients via a wide range of biogeochemical *** rates are controlled by abiotic factors,e.g.,salinity,temperature or solute ***,a series of incubation experiments,using flow-through reactors,were conducted to simulate the biogeochemical cycling of nitrate (NO_(3)^(-)) and phosphorus(P)in permeable sediments under different NO_(3)^(-) availability conditions(factor I)along a salinity gradient(admixture of river and seawater,factor Ⅱ).In an oligotrophic scenario,i.e.,unamended NO_(3)^(-) concentrations in both river and seawater,sediments acted as a permanent net source of NO_(3)^(-) to the water *** peak production rate occurred at an intermediate salinity(20).IncreasingNO_(3)^(-) availability in river water significantly enhanced netNO_(3)^(-) removal rates within the salinity range of 0 to 30,likely via the denitrification pathway based on the sediment microbiota *** this scenario,the most active removal was obtained at salinity of *** both river and seawater were spiked with NO_(3)^(-),the highest removal rate switched to the highest salinity(36).It suggests the salinity preference of the NO_(3)^(-) removal pathway by local denitrifiers(e.g.,Bacillus and Paracoccus)and that NO_(3)^(-) removal in coastal sediments can be significantly constrained by the dilution related NO_(3)^(-) *** with the obtained variation for NO_(3)^(-) reactions,permeable sediments acted as a sink of soluble reactive P in all treatments,regardless of salinity and NO_(3)^(-) input concentrations,indicating a possibility of P-deficiency for coastal water from the intensive cycling in permeable ***,the net production of dissolved organic carbon(DOC)in all treatments was positively correlated with the measured NO_(3)^(-) reaction rates,indicating that the DOC supply may not be the key factor for NO_(3)^(-) removal rates due

关键词： NO_(3)^(-)production and removal flow-through reactors salinity stable isotopes permeable sediments

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Sarcopenia in chronic viral hepatitis:From concept to clinical relevance

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World Journal of Hepatology 2023年第5期15卷 649-665页

作者： Marta Paula Pereira Coelho Pedro Alves Soares Vaz de Castro Thaís Pontello de Vries Enrico Antônio Colosimo Juliana Maria Trindade Bezerra Gifone Aguiar rocha luciana Diniz Silva Sciences Applied to Adult Health Care Post-Graduate Programme Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Medical Undergraduate Student Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Statistics Instituto de Ciências ExatasUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Biological Sciences Universidade Estadual do MaranhãoAçailândia 65715-000MaranhãoBrazil Post-Graduate Programme of Animal Science Universidade Estadual do MaranhãoSão Luiz do Maranhão 65.055-310MaranhãoBrazil Laboratory of Research in Bacteriology Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil Department of Internal Medicine Faculdade de MedicinaUniversidade Federal de Minas GeraisBelo Horizonte 30130-100Minas GeraisBrazil

Although the frequency of metabolic risk factors for cirrhosis and hepatocellular carcinoma(HCC)is increasing,chronic hepatitis B(CHB)and chronic hepatitis C(CHC)remain the most relevant risk factors for advanced liver disease *** addition to liver damage,hepatitis B virus(HBV)and hepatitis C virus(HCV)infections are associated with a myriad of extrahepatic manifestations including mixed cryoglobulinaemia,lymphoproliferative disorders,renal disease,insulin resistance,type 2 diabetes,sicca syndrome,rheumatoid arthritislike polyarthritis,and autoantibody ***,the list has grown to include *** of muscle mass or muscle function is a critical feature of malnutrition in cirrhotic patients and has been found in approximately 23.0%-60.0%of patients with advanced liver ***,among published studies,there is significant heterogeneity in the aetiologies of hepatic diseases and measurement methods used to determine *** particular,the interaction between sarcopenia,CHB and CHC has not been completely clarified in a real-world *** can result from a complex and multifaceted virus-host-environment interplay in individuals chronically infected with HBV or ***,in the present review,we provide an overview of the concept,prevalence,clinical relevance,and potential mechanisms of sarcopenia in patients with chronic viral hepatitis,with an emphasis on clinical outcomes,which have been associated with skeletal muscle loss in these patients.A comprehensive overview of sarcopenia in individuals chronically infected with HBV or HCV,independent of the stage of the liver disease,will reinforce the necessity of an integrated medical/nutritional/physical education approach in the daily clinical care of patients with CHB and CHC.

关键词： clinical hepatitis Sarcopenia

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Diet and intestinal bacterial overgrowth: Is there evidence?

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World Journal of Clinical Cases 2022年第15期10卷 4713-4716页

作者： Claudineia Souza Raquel rocha Helma Pinchemel Cotrim Programa de Pós graduacao em Medicina e Saúde Universidade Federal da BahiaSalvador 40110-060BABrazil Sciences of Nutrition Federal University of BahiaSalvador 40110-060BABrazil

The intestinal microbiota and its role in health and disease processes have been the subject of several *** is known that changes in the intestinal microbiota occur due to several factors,such as the use of medication,age,lifestyle and diseases,which can modify intestinal homeostasis and lead to excessive growth of bacteria in the small intestine,triggering a clinical condition called small bowel bacterial overgrowth(SIBO).Individuals with SIBO may present gastrointestinal symptoms ranging from nausea,diarrhea and/or constipation,and flatulence to distension and abdominal pain,resulting from poor absorption of nutrients or changes in intestinal *** gold-standard treatment is based on the use of antibiotics to eradicate bacterial *** studies have evaluated diets in the treatment of SIBO;however,the studies are of low methodological quality,making extrapolation of the results to clinical practice ***,there is still not enough scientific evidence to support a specific type of diet for the treatment of SIBO.

关键词： Small intestinal bacterial overgrowth Treatment Diet Nutrition

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Preliminary results of a new intrastromal corneal ring segment as a tissue saving procedure in photorefractive keratectomy to correct moderate to high myopia

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International Journal of Ophthalmology(English edition) 2020年第12期13卷 1955-1960页

作者： Sandro Coscarelli Pablo Rodrigues Guilherme rocha Leonardo Torquetti Ennio Coscarelli Eye Clinic Belo Horizonte 30140-001Brazil Escola Paulista de Medicina Sao Paulo 04023-062Brazil Hospital de Olhos de Brasilia Brasilia 70200-670Brazil Center of Excellence in Ophthalmology Para de Minas 35660-051Brazil

AIM:To evaluate the clinical results after implantation of a new intrastromal corneal ring segment(ICRS)associated with photorefractive keratectomy(PRK)to correct high myopia(HM)patients with thin ***:We evaluated 42 eyes of 23 HM patients that had ICRS implantation followed by *** mean age of patients was 29.1±7.12 y(range 18 to 40 years old).Uncorrected visual acuity(UCVA),best corrected visual acuity(BCVA),keratometry,spherical equivalent,pachymetry,and aberrometry were compared using ANOVA with repeated measurements evaluated preoperatively and at last follow-up visit after the *** refractive predictability and simulated/real corneal ablation were also ***:The mean follow-up time after PRK was 6.8±1.6 *** mean preoperative UCVA improved from 20/800 preoperative to 20/100 after ICRS and 20/35 after *** mean preoperative BCVA was 20/25(range from 20/30 to 20/20)and remained unchanged after ICRS *** the PRK the mean BCVA was 20/25(range from 20/30 to 20/20).The mean spherical equivalent decreased from-7.25±1.12(range-5.00 to-9.00)preoperatively to-3.32±1.0(range-2.00 to-5.00)postoperatively(P<0.001)after ICRS implantation and decreased from-2.44±1.51 preoperatively to 0.32±0.45(range-0.625 to 0.875)postoperatively(P<0.001)after *** change in BCVA and topographic astigmatism was statistically significant(P<0.0001).CONCLUSION:ICRS in HM associated with PRK can be a tissue saving procedure and an alternative surgical option for correction of moderate to high myopia.

关键词： high myopia intrastromal corneal ring segments photorefractive keratectomy

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Antimicrobial Resistance in <i>Enterococcus</i>sp Isolated from Soft Cheese in Southern Brazil

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Advances in Microbiology 2014年第3期4卷 175-181页

作者： luciana Furlaneto-Maia Kátia Real rocha Fernanda Carla Henrique Amanda Giazzi Márcia Cristina Furlaneto Department of Food Microbiology Technological Federal University of Paraná Londrina Brazil Department of Microbiology State University of Londrina Londrina Brazil

The aim of this research was to study the incidence of antibiotic resistance in 56 Enterococcus strains isolated from dairy products. The identification of enterococci was detected by polymerase chain reaction (PCR) using specific primers to E. faecalis, E. faecium, E. gallinarum and E. casseliflavus, and antibiotic resistance was tested by the disk diffusion method. The most prevalent species was E. faecium with a rate of 58.33%, followed by 27.77% E. faecalis, 11.11% E. casseliflavus and 2.7% E. gallinarum. Distribution of resistance was found in different species. All isolates were susceptible to chloramphenicol, ampicillin, imipenem and amoxicillin/clavulanic acid. In addition, isolates resistant to tetracyclin, nalidixic acid, amikacin, erythromycin, vancomycin and cephalothin were detected. A total of 66.6% of E. faecium and 58.3% of E. faecalis strain were resistant to multiple drugs. The van(A) gene was detected in 100% of vancomycin resistant enterococci. Considering the results of our study, dairy enterococci can be considered a potential source for dissemination of antibiotic resistances.

关键词： Enterococci Dairy Products Foods Antimicrobial Resistance

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