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Improvement of the Quality of Brazilian Conilon through Wet Processing: A Sensorial Perspective

Agricultural Sciences 2019年第3期10卷 395-411页

作者： lucas louzada pereira Aldemar Polonini Moreli Tais Rizzo Moreira Carla Schwengber Ten Caten Joao Paulo pereira Marcate Danieli Grancieri Debona Rogério Carvalho Guarconi Federal Institute of Education Science and Technology of Espirito Santo Venda Nova do Imigrante CampusCoffee Analysis and Research Laboratory—LAPCVenda Nova do ImigranteBrazil Federal University of Espírito Santo Center for Agrarian Sciences and EngineeringJerônimo MonteiroBrazil Federal University of Rio Grande do Sul Department of Production and Transportation EngineeringPorto AlegreBrazil Capixaba Institute for Technical Assistance Research and Extension—INCAPERDepartment of StatisticsDomingos MartinsBrazil

Conilon coffee represents almost 20% of all coffee production in the Brazilian territory;however, it is inexpressive in relation to the sensory quality when compared to Arabica coffee. It is noteworthy that the coffee from the species Coffea canephora PIERRE has a denser sensorial profile, is less sweet, less acidic and with strong pronunciation of the body at the time of tasting. This study has unprecedentedly applied the use of starter cultures (yeast) to optimize and modify the metabolic pathways and consequently the sensorial quality perceived by Q-graders. The experiments were conducted in a randomized block design with five replicates, in the 3 × 4 factorial scheme, with three fermentation times—24, 48 and 72 hours and four wet processing tests—Washed, Yeast fermentation, Fully washed without yeast and Fully washed with yeast. The sensorial results indicate a new perspective for the application of wet processing with starter cultures for Conilon coffee. They indicate gains in sensory scale, opening a new phase of studies on spontaneous and induced fermentation for Conilon coffee in Brazil and worldwide.

关键词： Spontaneous Fermentation Induced Fermentation Sensory Routes Conilon Coffee Q-Graders Sensory Aspects

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Sensory Profile of Fermented Arabica Coffee in the Perception of American Cupping Tasters

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Agricultural Sciences 2019年第3期10卷 321-329页

作者： lucas louzada pereira Rogério Carvalho Guarconi Tais Rizzo Moreira Dério Brioschi Jr. Joao Paulo pereira Marcate Luiz Henrique Bozzi Pimenta de Sousa Aldemar Polonini Moreli Danieli Grancieri Debona Carla Schwengber Ten Caten Federal Institute of Education Science and Technology of Espírito Santo Venda Nova do Imigrante CampusFood Science and Technology ProgramRua Elizabeth Minete PerimVitóriaBrazil Capixaba Institute for Technical Assistance Research and ExtensionINCAPERDepartment of StatisticsDomingos MartinsBrazil Department of Forest Sciences and Wood Federal University of Espirito SantoCenter for Agrarian Sciences and EngineeringJeronimo MonteiroBrazil Department of Production and Transportation Engineering Federal University of Rio Grande do SulPorto AlegreBrazil

The consumption of specialty coffees has been growing year after year, indicating that several niches and market forms according to the sensorial orientation of the consumers, as well as the cupping tasters and agents are responsible for the composition of blends, and for the physical and sensory classification of the coffees in the industry. This study presented 5 methods of spontaneous and induced fermentation to different North American cupping tasters, in order to evaluate coffee quality potential, based on the Specialty Coffee Association (SCA) protocol. During the months of May to July of 2017, 150 coffee samples were evaluated. Initial results indicate that North American cupping tasters evaluated dry fermented coffees using yeast culture as superiors, in the sensorial aspect, than the more conventional methods in Brazil. Such indication opens new discussions on the acceptance of a determined product, followed by the potential for modification of the sensory route as of the fermentation that will affect the attributes.

关键词： Arabica Coffee Fermentation Acceptance Cupping Tasters

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Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca^(2+) Signaling

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Chinese Journal of Integrative Medicine 2024年第6期30卷 525-533页

作者： lucas dos Santos Zamarioli Michele Rosana Maia Santos Adolfo Garcia Erustes Vanessa Marques Meccatti Thais Cristine pereira Soraya S Smaili Maria Cristina Marcucci Carlos Rocha Oliveira Gustavo JS pereira Claudia Bincoletto Department of Pharmacology Paulista School of MedicineFederal University of São PauloSão PauloSP04044-020Brazil Research Group on Phytocomplexes and Cell Signaling School of Health SciencesAnhembi Morumbi UniversitySão PauloSP03164-000Brazil Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State University(ICT-UNESP)São PauloSP12231-280Brazil

Objective To evaluate the chemical composition and effects of Artemisia vulgaris(AV)hydroalcoholic extract(HEAV)on breast cancer cells(MCF-7 and SKBR-3),chronic myeloid leukemia(K562)and NIH/3T3 *** Phytochemical analysis of HEAV was done by high-performance liquid chromatography-mass(HPLC)*** and cell death studies were performed using trypan blue and Annexin/FITC-7AAD,***-1(Fer-1)and necrostatin-1(Nec-1)were used to assess the mode of HEAV-induced cell death and acetoxymethylester(BAPTA-AM)was used to verify the involvement of cytosolic calcium in this *** calcium measurements were made using *** HEAV decreased the viability of MCF-7,SKBR-3 and K562 cells(P<0.05).The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts(P<0.05).Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD(P<0.05).The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone(P<0.01).HEAV-induced Ca^(2+)release mainly from lysosomes in K562 cells(P<0.01).Furthermore,BAPTA-AM,an intracellular Ca^(2+)chelator,decreased the number of non-viable cells treated with HEAV(P<0.05).Conclusions HEAV is cytotoxic and activates several modalities of cell death,which are partially dependent on lysosomal release of Ca^(2+).These effects may be related to artemisinin and caffeoylquinic acids,the main compounds identified in HEAV.

关键词： Artemisia vulgaris necroptosis ferroptosis late apoptosis Ca^(2+)signaling

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Effects of Imazethapyr-Based Herbicide Formulation in the Zebrafish (Danio rerio) Hepatocyte Cell Line (ZF-L): Cytotoxicity and Oxidative Stress

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Journal of Environmental Protection 2024年第6期15卷 747-765页

作者： Edila Maria Kickhöfel Ferrer Milena Mattes Cerveira Pedro Henrique Flores Da Cruz Cleiton Jesus Andrade pereira lucas Moraes Berneira Claudio Martin pereira de pereira Nathalia Stark Pedra Roselia Maria Spanevello Jean Carlos Bauer Vieira Clarissa Piccinin Frizzo Mateus Tavares Kütter Janice Luehring Giongo Rodrigo de Almeida Vaucher Center of Chemical Pharmaceutical and Food Sciences Microorganism Biochemistry and Molecular Biology Research Laboratory (LAPEBBIOM) Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Center of Chemical Pharmaceutical and Food Sciences Laboratory for Lipidomic and Bio-Organic Research Bioforensic Research Group Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Center of Chemical Pharmaceutical and Food Sciences Neurochemistry Inflammation and Cancer Laboratory Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Department of Chemistry Heterocycle Chemistry Center (NUQUIMHE) Federal University of Santa Maria (UFSM) Santa Maria Rio Grande do Sul Brazil Department of Microbiology and Parasitology Biology Institute Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil

Seizures of agrochemical formulations have increased in Brazil and Rio Grande do Sul is among the Brazilian states with the highest number of seizures of these products obtained illicitly. The use of illicit formulations can cause significant harm to agricultural production, the environment, and non-target species. This study evaluated the cytotoxicity and oxidative stress of a seized formulation containing the herbicide imazethapyr (IMZT). Characterization of the herbicide included gas chromatography-mass spectrometry (GC-MS) and thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)). Hemolytic and cytotoxicity assays in ZF-L hepatic cells showed IC50 values of 12.75 µg/mL, 3.01 µg/mL, 2.67 µg/mL, and 1.61 µg/mL for erythrocytes, [3(4,5-dimethyl)-2 bromide-5 diphenyl tetrazolium] (MTT), neutral red (NR), and lactate dehydrogenase (LDH) assays, respectively. The median IC50 of 2.84 µg/mL was used in oxidative stress assays, revealing increased reactive oxygen species (ROS) production, reduced total sulfhydryl content, and decreased superoxide dismutase (SOD) and catalase (CAT) activity. This study is the first to report in vitro oxidative stress induced by IMZT in the ZF-L cell line, emphasizing the importance of in vitro assays for assessing the toxic effects of seized agrochemicals on human health and the environment.

关键词： Agrochemical Imidazolinone Zebrafish Cell Viability Oxidative Damage

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Antimicrobial and synergistic effects of lemongrass and geranium essential oils against Streptococcus mutans,Staphylococcus aureus,and Candida spp.

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World Journal of Critical Care Medicine 2024年第3期13卷 63-74页

作者： Patrícia Michelle Nagai de Lima Thaís Cristine pereira Lara Steffany de Carvalho Letícia Ferreira dos Santos Carlos Eduardo Rocha Oliveira lucas de Paula Ramos Maria Cristina Marcucci Amjad Abu Hasna Luciane Dias de Oliveira Department of Biosciences and Oral Diagnosis Institute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000SPBrazil Anhembi Morumbi University School of MedicineSão Josédos Campos 12230-002SPBrazil Department of Restorative Dentistry Endodontics DivisionInstitute of Science and TechnologySão Paulo State UniversitySão Josédos Campos 12245000São PauloBrazil

BACKGROUND The oral cavity harbors more than 700 species of bacteria,which play crucial roles in the development of various oral diseases including caries,endodontic infection,periodontal infection,and diverse oral *** To investigate the antimicrobial action of Cymbopogon Schoenanthus and Pelargonium graveolens essential oils against Streptococcus mutans,Staphylococcus aureus,Candida albicans,***,and *** Minimum microbicidal concentration was determined following Clinical and Laboratory Standards Institute *** synergistic antimicrobial activity was evaluated using the Broth microdilution checkerboard method,and the antibiofilm activity was evaluated with the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide *** were analyzed by one-way analysis of variance followed by the Tukey post-hoc test(P≤0.05).RESULTS *** and *** essential oils were as effective as 0.12%chlorhexidine against *** and *** monotypic biofilms after 24 *** 24 h *** essential oil at 0.25%was more effective than the nystatin group,and *** essential oil at 0.25%was as effective as the nystatin *** *** and *** essential oils are effective against ***,***,***,***,and *** at different concentrations after 5 min and 24 h.

关键词： Antifungal effect Lemongrass Geranium Candida albicans Candida dubliniensis Candida krusei Staphylococcus aureus Streptococcus mutans

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Effects of submergence in water on seed germination and vigor of the Copaifera lucens(Fabaceae) seedlings

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Journal of Forestry Research 2014年第4期25卷 903-908页

作者： Daniela Baldez Vidal Isis Leite Andrade Eusinia louzada pereira Andrade Marcelo Schramm Mielke Department of Agricultural and Environmental Sciences State University of Santa Cruz BR 415 km 16 Ilhéus 45662-300 Bahia Brazil Department of Biological Sciences State University of Santa Cruz BR 415 km 16 Ilhéus 45662-300 Bahia Brazil

We analyzed the tolerance of Copaifera lucens seeds to sub- mersion in water to assess the use of this species for direct seeding in riparian forest restoration programs. Seeds were submerged in water for 2, 4, 8, 16 and 32 days or not submerged （control = 0 days of submer- gence）. For the control and at the end of each period of submersion, germination and seedling vigor tests were carried out. For germination tests, seeds were sown in plastic pots containing sand and kept in labora- tory conditions. The percentage of seed germination, the germination rate and the average germination time were analyzed. For seedlings, total biomass, leaf area, leaf mass per area and leaf area ratio were analyzed. Submersion time drastically affected the dissolved oxygen content and seed germination. Between 4 and 8 days of submersion there was a de- crease from 83.8% to 15.6% in the germination percentage. No seed germination occurred after 16 days of submersion. Although there was a significant decrease in the percentage of seed germination between 4 and 8 days of submersion, seedling vigor was not affected. Seeds of this species were partially tolerant to submersion in water, suggesting that C. lucens is a promising species for direct seeding in riparian forest restora- tion projects.

关键词： Copaiba Brazilian Atlantic Forest forest restoration germi-nation vigor

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On the impact of grinding conditions in the flotation of semi-soluble salt-type mineral-containing ores driven by surface or particle geometry effects?

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International Journal of Mining Science and Technology 2023年第7期33卷 855-872页

作者： lucas pereira Nathalie Kupka Duong Huu Hoang Bruno Michaux Sondos Saquran Doreen Ebert Martin Rudolph Helmholtz-Zentrum Dresden-Rossendorf Helmholtz Institute Freiberg for Resource TechnologyFreiberg 09599Germany Maelgwyn Mineral Services Ltd Cardiff CF244PAUnited Kingdom

Grinding and flotation processes are often studied independently, despite the well-established grinding influence on flotation performance, which affects not only particle size and thus liberation but also shape and leads to complex changes in pulp chemistry affecting the particle surface properties relevant for selective bubble attachment. Yet, no study jointly investigated these possible causes and many are limited to single mineral flotation. We relate grinding conditions to changes in pulp chemistry and particle surface properties and assess their impact on upgrading. We studied three non-sulfide ores with different feed grades and valuables: scheelite, apatite, and fluorite. These were dry-, wet-, and wet conditionedground before flotation in a laboratory mechanical cell. Results were evaluated with bulk-and particle-specific methodologies. The selectivity of the process is higher after dry grinding for the fluorite and apatite ores and irrelevant for the scheelite ore. Variations in flotation kinetics of individual particles associated to their size and shape are not sufficient to explain these results. The higher concentration of Ca2+and Mg2+observed in the pulp after wet grinding, altering particle surface properties, better explains the phenomenon. Additionally, we demonstrate how particle shape impacts are system specific and related to both entrainment and true flotation.

关键词： Grinding-flotation interactions Semi-soluble salt-type minerals Geometallurgy Particle-based separation modelling Surface interactions

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Testing the robustness of particle-based separation models for the magnetic separation of a complex skarn ore

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International Journal of Mining Science and Technology 2022年第3期32卷 645-655页

作者： lucas pereira Max Frenzel Markus Buchmann Marius Kern Raimon Tolosana-Delgado K.Gerald van den Boogaart Jens Gutzmer Hemholtz-Zentrum Dresden-Rossendorf Helmholtz Institute Freiberg for Resource Technology09599 FreibergGermany German Aerospace Center Institute of Low-Carbon Industrial Processes03046 CottbusGermany TU Bergakademie Freiberg Institute for Stochastics09599 FreibergGermany

Physical separation processes are best understood in terms of the behaviour of individual ore ***,while different empirical particle-based separation modelling approaches have been developed,their predictive performance has never been tested under variable process ***,we investigated the predictive performance of a state-of-the-art particle-based separation model under variable feed composition for a laboratory-scale magnetic separation of a skarn *** scenarios were investigated:one in which the mass flow of the different processing streams could be measured and one in which it had to be estimated from *** both scenarios,the predictive models were sufficiently general to predict the process outcomes of new samples of variable ***,the scenario in which mass flow could be measured was4%more precise in predicting mass *** process behaviour of minerals present at concentrations above 0.1%by weight could be accurately *** findings indicate the potential use of this method to minimize the costs of metallurgical testwork while providing in-depth understanding of the recovery behaviour of individual ore ***,the method may be used to establish powerful tools to forecast mineral recoveries for partly new ore types at a running mining operation.

关键词： Metallurgical tests Particle-based separation modelling Magnetic separation Cassiterite recovery

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Flight performance and wing morphology in the bat Carollia perspicillata:biophysical models and energetics

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Integrative Zoology 2023年第5期18卷 876-890页

作者： lucas de Oliveira CARNEIRO Breno MELLADO Marcelo Rodrigues NOGUEIRA Ariovaldo pereira da CRUZ-NETO Leandro Rabello MONTEIRO Laboratório de Ciências Ambientais Centro de Biociências e BiotecnologiaUniversidade Estadual do Norte FluminenseCampos dos GoytacazesRio de JaneiroBrasil Departamento de Biodiversidade Universidade Estadual PaulistaRio ClaroSão PauloBrasil

Studies on functional performance are important to understand the processes responsible for the evolution of *** trait variation within species influences the energetic cost of locomotion and impacts life history traits,with ecological and evolutionary *** study examined wing morphology correlates of flight performance measured by energetic expenditure in the Seba’s short-tailed bat,Carollia *** the flight experiments,nature caught bats(59 females,57 males)were allowed to fly for 3 min in a *** each flight,thermographic images were taken to measure body temperature,and biophysical models were used to calculate sensible heat loss as a measure of energetic *** morphological traits were measured for each individual and associated with heat loss and power required to fly on performance *** morphological traits explained 7-10%of flight energetic cost,and morphologies with the best performance would save the energy equivalent to 9-30%of total daily *** optimal performance areas within the *** morphospace were consistent with predicted selection trends from the literature.A trade-off between demands for flight speed and maneuverability was *** loading and camber presented sexual *** morphological differences are likely associated with more economical but less maneuverable flight in females,leading them to fly more often in open areas along the forest *** findings demonstrate how small scale changes in wing morphology can affect life history strategies and fitness.

关键词： aerodynamics Chiroptera infrared thermography performance surfaces Phyllostomidae wing morphometrics

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Inter-Individual Rapid Force Improvements after Mixed Session and Traditional Periodization in Aging Adults:A Randomized Trial

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Journal of Science in Sport and Exercise 2021年第2期3卷 125-137页

作者： Bruno Monteiro Moura Ewertton de Souza Bezerra lucas B.R.Orssatto Antonio Renato pereira Moro Fernando Diefenthaeler Universidade Federal de Santa Catarina Centro de DesportosLaboratório de BiomecânicaFlorianópolisCEP 88040-900Brazil Universidade Federal do Amazonas Faculdade de Educação Física e FisioterapiaLaboratório de Estudo do Desempenho HumanoManausCEP 38400-678Brazil School of Exercise and Nutrition Sciences Queensland University of TechnologyBrisbane 4059Australia

Background Different periodization models have been widely used to improve maximum and rapid force in aging ***,it is unclear if some specific model can influence the remarkable heterogeneity of responsiveness to rapid torque improvements following resistance *** Compare the effects of mixed session periodization(MSP)and traditional(TP)models on neuromuscular performance and individual responsiveness in aging *** Twenty-two aging men and women(64.6±5.2 years old;1.68±0.1 m;77.8±16.0 kg)completed the intervention *** were assigned into MSP(n=11)or TP(n=11)resistance training for lower *** outcome measures normalized dynamic leg press(5-RM:BM),absolute and normalized knee isometric peak torque(PT and PT:BM)and rate of torque development,and impulse were assessed at baseline and after three blocks of a 9-wk resistance training(3 sessions per week).Thigh leg lean mass was assessed by dual-energy x-ray absorptiometry at baseline and after the *** Similar increases were observed for 5-RM:BM(MSP:28.8%±13.5%and TP:26.4%±7.6%,P0.05).Absolute and normalized RTD remained unchanged throughout intervention(P>0.05),but both groups improved impulse(MSP:22.1%±25.7%and TP:12.6%±45.2%,P<0.001).Only MSP responders(n=5)presented significant time effect for absolute RTD(P<0.05)and impulse(P<0.05).Conclusion Although both periodization protocols increased 5-RM:BM and PT,it was not interchangeable through improve-ments in RTD even adopting power training ***,these results suggest a great inter-individual variability following different periodization models for aging people.

关键词： Rate of torque development Impulse Strength training Muscular strength Hypertrophy Periodization

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