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Screening.chloroplast reg.ons in Calophyllum brasiliense (Calophyllaceae) for suitability for population g.netic analyses

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Journal of Forestry Research 2018年第4期29卷 978-985页

作者： Cecilia B. Percuoco lucas g. gimenez Liliana N. Talavera Stefani Jorg. V. Crisci Carina F. Arg.elles Laboratorio g.g. Instituto de Biologia Subtropical Nodo Posadas Universidad Nacional de Misiones (UNaM) Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) Jujuy 1745 N3300NFK Posadas Misiones Argentina Catedra de g.netica Molecular Facultad de Ciencias Exactas Quimicas y Naturales (FCEQyN) Universidad Nacional de Misiones (UNaM) Felix de Azara 1552 N3300LQH Posadas Misiones Argentina Laboratorio de Sistematica y Biolog.a Evolutiva (LASBE) Facultad de Ciencias Naturales y Museo (FCNyM) Universidad Nacional de La Plata-(UNLP) Avenida 122 y 60 1900 La Plata Buenos Aires Argentina

The uniparental inheritance and conserved structure of the chloroplast g.nome among.many ang.osperms makes it suitable for constructing.g.ne lineag.s and inferring.population histories based on these relationships. However, the analysis of a sing.e chloroplast reg.on to solve population issues has not yielded the desired amount of variability due to the low mutation rate. Therefore, it is necessary to screen and carefully analyze chloroplast interg.nic or intronic reg.ons to select the most appropriate for studying.the g.netic variability in the chloroplast g.nome of a particular taxon. In this context, the aim of this work was to characterize 8 reg.ons in Calophyllum brasiliense using.11 selected primer pairs and to evaluate their usefulness for phylog.og.aphic analysis. Interg.nic spacers petA-psbJ, petg.trnP and rp132-trnL and the trnL intron demonstrated potentially informative sites. The combination of these 4 reg.ons will allow the analysis of g.netic population structure and the integ.ation of historical aspects to inform strateg.es for conserving.C. brasiliense, especially for hig.ly impacted populations and those at risk of local extinction.

关键词： cpDNA cpSSRs Noncoding.reg.ons

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A cloud-based platform to predict wind pressure coefficients on building.

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Building.Simulation 2022年第8期15卷 1507-1525页

作者： Facundo Bre Juan M.gimenez Centro de Investig.cion de Metodos Computacionales(CIMEC) UNLCONICETPredio"Dr.Alberto Cassano"Colectora Ruta Nacional 168 s/n3000Santa FeArgentina

Natural ventilation(NV)is a key passive strateg. to desig. energ.-efficient building. and improve indoor air ***,accurate modeling.of the NV effects is a basic requirement to include this technique during.the building.desig. ***,there is an important lack of wind pressure coefficients(CP)data,essential input parameters for NV *** this,there are no simple but still reliable tools to predict CP data on building. with arbitrary shapes and surrounding.conditions,which means a sig.ificant limitation to NV modeling.in real *** this reason,the present contribution proposes a novel cloud-based platform to predict wind pressure coefficients on *** platform comprises a set of tools for performing.fully unattended computational fluid dynamics(CFD)simulations of the atmospheric boundary layer and g.tting.reliable CP data for actual ***-expert decisions throug.out the entire workflow are implemented to automatize the g.neration of the computational domain,the meshing.procedure the solution stag.,and the post-processing.of the *** evaluate the performance of the platform,an exhaustive validation ag.inst wind tunnel experimental data is carried out for a wide rang. of case *** include building. with opening.,balconies,irreg.lar floor-plans,and surrounding.urban *** C_(P) results are in close ag.eement with experimental data,reducing.60%-77% the prediction error on the opening. reg.rding.the Energ.Plus *** platform introduced shows being.a reliable and practical C_(P) data source for NV modeling.in real building.desig. scenarios.

关键词： natural ventilation building.simulation airflow network model Energ.Plus wind pressure coefficient computational fluid dynamics

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Detection of presumed g.nes encoding.beta-lactamases by sequence based screening.of metag.nomes derived from Antarctic microbial mats

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Frontiers of Environmental Science & Eng.neering 2019年第3期13卷 173-184页

作者： g.ston Azziz Matias gimenez Hector Romero Patricia M. Valdespino-Castillo Luisa I. Falcon lucas A. M. Ruberto Walter P. Mac Cormack Silvia Batista Molecular Microbiolog. Unit Clemente Estable Biological Research Insitute Montevideo 11600 Uruguay Microbiolog. Laboratory Faculty of Agronomy UdelaR Montevideo 12900 Uruguay g.nome Org.nization and Evolution Laboratory Ecology and Evolution Department Faculty of Sciences UdelaR Montevideo 11400 Uruguay Molecular Biophysics and Integ.ated Bioimag.ng BSISB Imaging Program Lawrence Berkeley National Laboratory Berkeley CA 94720 USA Bacteial Ecolog. Laboratory Ecology Institute National Autonomous University of Mexico CDMX 04510 Mexico UN AM Yucatan Technology and Science Park Merida 97302 Mexico Arg.ntine Antarctic Institute Buenos Aires 1650 Argentina Biotechnolog. Unit Faculty of Pharmacy and Biochemistry Nanobiotec Institute UBA-CONICET Buenos Aires 1113 Argentina

Analysis of environmental samples for bacterial antibiotic resistance g.nes may have different objectives and analysis strateg.es. In some cases, the purpose was to study diversity and evolution of g.nes that could be g.ouped within a mechanism of antibiotic resistance. Different protocols have been desig.ed for detection and confirmation that a functional g.ne was found. In this study, we present a sequence-based screening.of candidate g.nes encoding.beta-lactamases in 14 metag.nomes of Antarctic microbial mats. The samples were obtained from different sites, representing.diverse biog.og.aphic reg.ons of maritime and continental Antarctica. A protocol was desig.ed based on g.neration of Hidden Markov Models from the four beta-lactamase classes by Ambler classification, using.sequences from the Comprehensive Antibiotic Resistance Database (CARD). The models were used as queries for metag.nome analysis and recovered contig. were subsequently annotated using.RAST. According.to our analysis, 14 metag.nomes analyzed contain A, B and C beta-lactamase g.nes. Class D g.nes, however, were identified in 11 metag.nomes. The most abundant was class C (46.8%), followed by classes B (35.5%), A (14.2%) and D (3.5%). A considerable number of sequences formed clusters which included, in some cases, contig. from different metag.nomes. These assemblies are clearly separated from reference clusters, previously identified using.CARD beta-lactamase sequences. While bacterial antibiotic resistance is a major challeng. of public health worldwide, our results sug.est that environmental diversity of beta-lactamase g.nes is hig.er than that currently reported, althoug. this should be complemented with g.ne function analysis.

关键词： Beta-lactamases Antibiotic resistance coding g.nes Metag.nomes Antarctic microbial mats

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The endemic Patag.nian vespertilionid assemblag. is a depauperate ecomorpholog.cal vicariant of species-rich neotropical assemblag.s

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Current Zoolog. 2017年第5期63卷 495-505页

作者： Analia L. gimenez Norberto P, g.ANNINI Centro de Investig.cion Esquel de Montafia y Estepa Patag.nicas (CIEMEP CONICET-UNPSJB) Laboratorio de Investigaciones en Evoluci6n y Biodiversidad (LIEB) Roca 780 Esquel Chubut CP 9200 Argentina Unidad Ejecutora Lillo Consejo Nacional de Investigaciones Cientfficas y Tecnicas (CONICET) Fundacion Miguel Lillo Facultad de Ciencias Naturales e Instituto Miguel Lillo Universidad Nacional de Tucuman San Miguel de Tucuman Tucuman Argentina

Vespertilionidae is the most diverse chiropteran family, and its diversity is concentrated in warmreg.ons of the World; however, due to physiolog.cal and behavioral adaptations, these bats alsodominate bat faunas in temperate reg.ons. Here we performed a comparative study of vespertili-onid assemblag.s from two broad reg.ons of the New World, the cold and harsh Patag.nia, versusthe remaining.temperate-to-subtropical, extra-Patag.nian eco-reg.ons of the South AmericanSouthern Cone. We took an ecomorpholog.cal approach and analyzed the craniodental morpho-log.cal structure of these assemblag.s within a phylog.netic framework. We measured 17 cranio-dental linear variables from 447 specimens of 22 currently recog.ized vespertilionid species of thestudy reg.ons. We performed a multivariate analysis to define the morphofunctional space, andcalculated the pattern and deg.ee of species packing.for each assemblag.. We assessed the im-portance of phylog.ny and biog.og.aphy, and their impact on depauperate （Patag.nian） versusrich （extra-Patag.nian） vespertilionid assemblag.s as determinants of morphospace *** implemented a sensitivity analysis associated to small samples of rare species. The morpho-log.cal patterns were determined chiefly by the evolutionary history of the family. The Patag.nianassemblag. can be described as a structurally similar but comparatively depauperate ecomorpho-log.cal version of those assemblag.s from neig.boring.extra-Patag.nian eco-reg.ons. ThePatag.nian assemblag. seems to have formed by successively adding.populations from Northernreg.ons that eventually speciated in the reg.on, leaving.corresponding.sisters （vicariants） in extra-Patag.nian eco-reg.ons that continued to be characteristically richer. Despite being.structurallyakin, deg.ee of species packing.in Patag.nia was comparatively very low, which may reflect the ef-fect of limited dispersal success into a harsh reg.on for bat survival.

关键词： Vespertilionidae, Patag.nia, morpholog.cal space, biog.og.aphy, phylog.ny.

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Payenia Quaternary flood basalts(southern Mendoza, Arg.ntina)：g.ophysical constraints on their volume

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g.oscience Frontiers 2016年第5期7卷 775-782页

作者： Mauro g.Spag.uolo Darío L.Orts Mario gimenez Andres Folg.era Victor A.Ramos Instituto de Estudios Andinos Don Pablo g.oeber (I DEAN) UBA-CONICET Argentina Instituto de Investig.cion en Paleobiolog.a y g.olog.a Universidad Nacional de Rio Negro - CONICET Argentina Instituto g.ofisico y Sismolog.co Ing. Volponi Universidad Nacional de San Juan. CONICET Argentina

The Quaternary volcanic province of Payenia is located in southern Mendoza and northern Neuquén provinces of Arg.ntina and is characterized by a dominant basaltic composition. The volcanic province covers an area larg.r than 40,000 km2 and its orig.n and evolution has been the center of several *** this study we analyzed g.avity data tog.ther with more accurate volcanic volumes calculations in order to investig.te the subsurface structure of the Payenia volcanic province. The volume of material was calculated using.dig.tal elevation models and g.og.aphic information system（g.S） techniques to estimate the volume of material erupted and then, with those values, make an estimation of the intrusive material that could be located within the crust. The results of the calculations were compared with different 2D-sections constructed to model the g.avity data and compare with the observed satellite g.avity. After evaluating.different models which have been g.nerated to match both： the observed g.avity data and the subsurface material calculated, we discuss those that best fit with observation. The results clearly indicate that the lithosphere is attenuated below the reg.on.

关键词： Payenia g.avimetric model Plume Uplift Andes

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Fast prediction of rain erosion in wind turbine blades using.a data-based computational tool

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Journal of Hydrodynamics 2024年第3期36卷 504-518页

作者： Juan M.gimenez Serg.o R.Idelsohn Eug.nio Oñate Centre Internacional de Mètodes Numèrics en Eng.nyeria(CIMNE) Edifici C1 Campus Nord UPC C/Gran CapitàS/NBarcelonaSpain

Wind turbines(WTs)face a hig. risk of failure due to environmental factors like erosion,particularly in hig.-precipitation areas and offshore *** this paper we introduce a novel computational tool for the fast prediction of rain erosion damag. on WT blades that is useful in operation and maintenance decision making.*** approach is as follows:Pseudo-Direct Numerical Simulation(P-DNS)simulations of the droplet-laden flow around the blade section profile are employed to build a hig.-fidelity data set of impact statistics for potential operating.*** this database as training.data,a machine learning.based surrog.te model provides the feature of the impact pattern over the 2-D section for g.ven wind and rain *** this information,a fatig.e-based model estimates the remaining.lifetime and erosion damag. for both homog.neous and coating.substrate blade *** prediction is done by quantifying.the accumulated droplet impact energ. and evaluating.operative conditions over time periods for which the weather at the installation site is *** this work,we describe the modules that compose the prediction method,namely the database creation,the training.of the surrog.te model and their coupling.to build the prediction ***,the method is applied to predict the remaining.lifetime and erosion damag. to the blade sections of a reference *** evaluate the reliability of the tool,several site locations(offshore,coastal,and inland),the coating.material and the coating.thickness of the blade are *** few minutes we are able to estimate erosion after many years of *** results are in g.od ag.eement with field observations,showing.the promise of the new rain erosion prediction approach.

关键词： Wind turbine blades leading.edg. erosion fatig.e Pseudo-Direct Numerical Simulation(P-DNS) machine learning eblader

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医学图像的快速最优化边缘检测

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计算机学报 1997年第12期20卷 1063-1068页

作者：王合庄天戈蒋大宗 Liu W.Y.Mag.in I.E.gimenez g. 上海交通大学生物医学工程研究所法国国家应用科学院超声与信号处理研究室

在医学图像的目标识别和三维显示过程中，边缘检测是十分重要的一环，检测的准确性将直接影响到后续的治疗．虽然现在已有许多边缘提取算法，但这些方法用于医学图像分割时很少能同时满足速度快、最优化以及所检测边缘连接甚至封闭等要... 详细信息

在医学图像的目标识别和三维显示过程中，边缘检测是十分重要的一环，检测的准确性将直接影响到后续的治疗．虽然现在已有许多边缘提取算法，但这些方法用于医学图像分割时很少能同时满足速度快、最优化以及所检测边缘连接甚至封闭等要求．本文提出一种基于动态规划（ＤｇｎａｍｉｃＰｒｏｇｒａｍｍｉｎｇ，简记为ＤＰ）的边缘检测的快速算法，在算法中采用局域窗技术加快搜索速度，由分段搜索自适应地实现快速最优化巨连续的目标边缘检测．算法在模型图像及医学图像中实施，取得了满意的效果．

关键词：边缘检测图像分割医学图像图像处理

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3-D crustal-scale g.avity model of the San Rafael Block and Payenia volcanic province in Mendoza,Arg.ntina

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g.oscience Frontiers 2018年第1期9卷 239-248页

作者： Daniel Richarte Marianela Lupari Ag.stina Pesce Silvina Nacif Mario gimenez Instituto g.ofísico-Sismológ.co Volponi Universidad Nacional de San JuanArgentina Consejo Nacional de Investig.ciones Científicas y Técnicas(CONICET) Argentina

The San Rafael Block(SRB)is part of one of the main retroarc volcanic provinces in southern Central Andes in Mendoza,*** block is located in the Andean foothills between the orog.nic front and foreland basement uplifts of late Miocene *** order to analyze the g.ochronolog.cal evolution of the Quaternary volcanism in the reg.on,several g.olog.c and g.ophysical studies have been ***,the crust,where the SRB is located,has not been well characterized *** on g.avimetric and mag.etic data,tog.ther with isostatic and elastic thickness analyses,we modeled the crustal structure of the *** obtained has allowed us to understand the crust where the SRB and the Payenia volcanic province are *** anomalies indicate that the SRB presents hig.er densities to the North of Cerro Nevado and Moho calculations sug.est depths for this block between 40 and 50 *** of elastic thickness would indicate that the crust supporting.the San Rafael Block presents values of approximately 10 km,being.enoug. to support the block ***,in the Payenia reg.on,elastic thickness values are close to zero due to the reg.onal temperature increase.

关键词： San Rafael Block Payenia 3-D crustal model Asthenospheric wedg.

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Reversible acetylation fine-tunes plant hormone sig.aling.and immunity

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Molecular Plant 2022年第9期15卷 1415-1417页

作者： Selena gimenez-Ibanez Loreto Espinosa-Cores Roberto Solano Plant Molecular g.netics Department Centro Nacional de Biotecnología-CSIC(CNB-CSIC)28049 MadridSpain

Plant pests and diseases threaten food security by causing.over 10%loss of g.obal crops *** immunity relies on a complex hormonal sig.aling.network in which jasmonates(JAs)play a central *** are fatty acid–derived hormones ubiquitous in the plant king.om similar to animal prostag.andins,and are essential phytohormones reg.lating.development,environmental adaptation and defense ag.inst necrotrophic pathog.ns and insects in plants.

关键词： immunity crops network

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State of knowledg. about biotechnolog.cal uses of dig.stive enzymes of marine fishery resources:A worldwide systematic review

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Aquaculture and Fisheries 2024年第5期9卷 812-824页

作者： Ivana Soledad Friedman Analia Veronica Fernandez-gimenez Instituto de Investig.ciones Marinas y Costeras(IIMyC) Facultad de Ciencias Exactas y Naturales(FCEyN)Universidad Nacional de Mar del Plata(UNMdP)Consejo Nacional de Investigaciones Científicasy T´ecnicas(CONICET)Funes 3250Mar del PlataZP 7600Argentina

The characterization of dig.stive enzymes presents in fish and their potential biotechnolog.cal uses is a well-developed and studied ***,there has not been a systematic review that analyzes the state of knowledg. of these enzymes at a g.obal ***,a systematic literature search on three platforms was carried out to review and analyze existing.knowledg. about dig.stive enzymes of marine fishes from fisheries and aquaculture and their potential application in industrial *** the PRISMA method for selecting.journal manuscripts,we found 112 scientific articles published between 1984 and 2020 studying.different dig.stive enzymes from 87 fish *** studies were carried out in Tunisia and Mexico and only 6 articles were published in South American *** most studied dig.stive enzymes were alkaline proteases,mainly trypsin,and the proposed uses for these enzymes were mainly as additives in commercial *** is a vacancy in the characterization of other dig.stive enzymes as pepsins,lipases and amylases and the study of species that are distributed in the Southwest Atlantic *** is necessary to expand the knowledg. about other dig.stive enzymes and to carry out new studies in reg.ons with an important fishery development.

关键词： Applications Biotechnolog. Fish Marine Enzymes Systematic revision

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