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Soybean Improvement for Lipoxygenase-free by Simple Sequence Repeat(SSR)Markers Selection

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Journal of Botanical Research 2021年第1期3卷 23-31页

作者： Valeria Carpentieri-Pipolo Thales pereira Barreto Daiana Alves da silva Ricardo Vilela Abdelnoor silvana Rockenbach Marin Giuliano Degrassi Embrapa Trigo P.O.Box 451Zip Code 99001-970 Passo FundoRio Grande do SulBrazil Syngenta Proteção de Cultivos LTDA.São Paulo SPBrazil Agronomic Institute(IAC)-Center for Grain and Fiber-Zip Code 13075-630-Campinas SPBrazil Embrapa Soja Zip Code 86001970LondrinaPRBrazil International Centre for Genetic Engineering and Biotechnology(ICGEB) Industrial Biotechnology GroupParque Tecnológico MigueleteSan MartínBuenos AiresRepública Argentina

Beany flavor of soybean(Glycine max(L.)Merr.)is caused by oxidation of polyunsaturated fatty acids by the action of three lipoxygenases(LOX1,LOX2 and LOX3)present in mature *** unpleasant flavor restricts human consumption of soybean *** problem could be solved through genetic elimination of alleles that code these *** cultivars and two hybrid population were selected and analyzed using genetic markers for alleles locus,encoding L_(ox1),L_(ox2)and L_(ox3)*** SSR marker Satt 212 confirmed the presence of the homozygous null-allele L_(x3)in the cultivar BRS 213,which were used for hybridization with BR *** F1 hybrid plants and homozygous L_(x3)lines in F_(2)segregating populations were successfully *** SSR markers Sat090 and Sat417 were the most effective diagnostic markers among all SSR markers ***090 and Satt417 confirmed the presence of the homozygous L_(x2)null-allele in the parental cultivar BRS 213 by flanking L_(x2)loci at 3,00 and 2,77 cM,*** presence of L_(x2)null allele in the F_(2)segregating populations between BRS 213 and BRS 155 was successfully identified with a selection efficiency of 98%and have great potential for further application in the Brazilian breeding program aimed at improving soybean seed quality.

关键词： Glycine max Lipoxygenase isozymes Molecular markers MAS

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

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Agricultural Sciences 2015年第3期6卷 295-303页

作者： Leticia Sayuri Murate Admilton Goncalves de Oliveira Allan Yukio Higashi Andre Riedi Barazetti Ane Stefano Simionato Caroline Santos da silva Glenda Cavalari Simoes Igor Matheus Oliveira dos Santos Marlon Renan Ferreira Martha Viviana Torres Cely Miguel Octavio Pérez Navarro Vanessa Fogaca de Freitas Marco Antonio Nogueira Joao Carlos Palazzo de Mello Rui pereira Leite Jr. Galdino Andrade Department of Microbiology Londrina State UniversityLondrinaBrazil Embrapa Soja LondrinaBrazil Department of Pharmacy and Pharmacology Maringa State UniversityMaringáBrazil Plant Protection Agronomic Institute of ParanáIAPARLondrinaBrazil

This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions’ activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrationswere tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL-1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p<0.05), reducing the incidence of citrus canker lesions.

关键词： X.axonopodis pv.citri Citrus sinensis Antibiosis Biological Control Natural Antibiotic

Altered Expression in Patients with Heart Failure of Circulating MicroRNAs Related to Lipoprotein Metabolism

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Journal of Pharmacy and Pharmacology 2018年第5期6卷 466-474页

作者： Priscila O. Carvalho Fatima R. Freitas ana Elisa M. Martinelli Bruna M. O. silva Milena N. C. Curiati Roberto Kalil-Filho Antonio C. pereira Barretto Raul C. Maranhao Lipid Metabolism Laboratory Heart Institute (InCor) Medical School Hospital University of Sao Paulo Sao Paulo 05403-000Brazil Santa Marcelina Hospital Sao Paulo 08270-070 Brazil Prevention and Rehabilitation Service Heart Institute (InCor) Medical School Hospital University of Sao Paulo So Paulo 05403-000 Brazil Faculty of Pharmaceutical Sciences University of Sao Paulo Sao Paulo 05508-000 Brazil

Unprecedented Low-Cost Hybrid Material for CO2 and CH4 Separation

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Journal of Chemistry and Chemical Engineering 2015年第5期9卷 318-323页

作者： Murilo pereira Moises Joziane Gimenes Meneguin Cleiser Thiago pereira da silva Vicente Lira Kupfer Pedro Augusto Arroyo Andrelson Wellington Rinaldi Emerson Marcelo Girotto Silvia luc.ana Favaro Eduardo Radovanovic Department of Chemical State University of Maring6 Maringa 87020-900 Brazil Department of Chemical Engineering State University of Maringa Maring6 87020-900 Brazil Department of Mechanical Engineering State University of Maringa Maring6 87020-900 Brazil Federal University Technological of Parana Apucarana 86812-460 Brazil

The zeolite synthesis using waste as silicon source is a promising low-cost process. Sugarcane bagasse ash is a hazardous solid waste generated in large amounts in the sugar industry. Thus, development of new procedures for its productive reuse is relevant. Therefore, this waste was used to prepare a new ZCNT (zeolite/carbon nanotube material). In this work, ZCNT was applied in a CO2/CH4 separation. The adsorption took place at 303 K under pressure of 0.1 until 20 bar and the data were fitted by Langmuir, Toth and Freundlich equations. The adsorbed amount at 20 bar found was 4.7 mmol of CO2/g and 4.1 mmol of CH4/g; at 3 bar the adsorbed amount was 4.1 mmol of CO2/g and 1.2 mmol of CH4/g. This result indicates that ZCNT has potential application in CO2/CH4 separation at low pressures.

关键词：低成本工艺 CH4 CO2 分离混合材料 Freundlich方程危险固体废物碳纳米管材料

Long-term Observation of Nighttime Clouds over Sao Joao do Cariri （7.4° S, 36.5° W）

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Journal of Environmental Science and Engineering(A) 2017年第9期6卷 431-438页

作者： Joyrles Femandes de Moraes Igo Paulino Edyvania Emily pereira Martins Amauri Fragoso de Medeiros ana Roberta Paulino Ricardo Arlen Buriti Department of Physics Federal University of Campina Grande (UFCG) Campina Grande 58429-900 Brazil

Factors Associated with Umbilical Cord Stump Healing Practices: Literature Review

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Open Journal of Obstetrics and Gynecology 2020年第8期10卷 1067-1078页

作者： Marilya Rodrigues Reis Marília Cordeiro de Sousa Lays de Souza silva Lívia Roberta Rodrigues Conceição Marcela de Andrade Silvestre Camila de Pina Soares Sudário Renata Calciolari Rossi Janaína Valadares Guimarães Thaíla Corrêa Castral Karina Machado Siqueira Pedro Teixeira Meireles Bruno Belmonte Martineli Gomes George Kemil Abdalla Douglas Reis Abdalla ana Karina Marques Salge Faculty of Nursing Federal University of Goiás Goiania Brazil UNIOESTE Presidente Prudente Brazil Medicine Course University of Uberaba Uberaba MG Brazil Biomedicine University of Sao Paulo Ribeirao Preto Brazil Health Sciences Faculty of Human Talents Uberaba Brazil

Objective: To analyze the evidence in the evolution of scientific production on the factors associated with curative practices of the umbilical stump. Materials and Methods: The study data collection took place in April 2017. The electronic databases used to search the articles were: PubMed (National Library of Medicine and National Institutes of Health) and VHL (Virtual Health Library). We used the keywords “perinatal care”, “umbilical cord”, “patient care team”, “neonatology”, “newborn”, in the Portuguese, English and Spanish languages, accompanied by the expression AND and selected through DeCS (Descriptors in Health Sciences). Results: A final sample of 9 articles was obtained from the PubMed and VHL databases, which met the inclusions criteria. It has been found that traditional umbilical stump care practices are still present in many communities, however, have been replaced by appropriate practices, the most recommended being: chlorhexidine 4% sanitation, soap and water and alcohol antisepsis 70%. Conclusions: There is a need for multi-professional involvement, approaching these women since pregnancy, with detailed information and accessible language about proper care with the newborn and the umbilical stump.

关键词： Perinatal Care The Umbilical Cord Patient Care Team Neonatology Newborn

Theranostics:纳米热疗法或可软化肿瘤,提高癌症靶向疗法的治疗效率

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现代生物医学进展 2017年第3期17卷 I0003-I0003页

作者： Iris Marangon Amanda A. K. silva Thomas Guilbert Jelena Kolosnjaj-Tabi Carmen Marchiol Sharuja Natkhunarajah Foucault Chamming’s Cécilia Ménard-Moyon Alberto Bianco Jean-luc Gennisson Gilles Renault Florence Gazeau Laboratoire Matière et Systèmes Complexes (MSC) CNRS-UMR 7057 Université Paris Diderot Sorbonne Paris Cité 10 rue Alice Domon et Léonie Duquet F-75205 Paris Cedex 13 France Plateforme Imagerie Institut Cochin Inserm U1016-CNRS UMR8104-Université Paris Descartes Sorbonne Paris Cité Paris 22 rue Méchain 75014 Paris France PARCC Inserm U970 Paris Cardiovascular Research Center-PARCC / Université Paris-Descartes Sorbonne Paris Cité 56 rue Leblanc 75015 Paris France University of Strasbourg CNRS Immunopathology and Therapeutic Chemistry UPR 3572 67000 Strasbourg France Institut Langevin CNRS-UMR 7587 1 place Jussieu 75205 Paris France.✉ Corresponding author: Florence GAZEAU rf.toredid-sirap-vinu@uaezag.ecnerolf. Tel: +33 (0)1 57 27 62 03 Fax: +33 (0)1 57 27 62 11.Competing Interests: The authors have declared that no competing interest exists.

肿瘤的机械阻力和标准疗法的附带损伤往往会阻碍抵御癌症的效果，近日，来自法国国家健康与医学研究所等机构的研究人员通过对肿瘤加热成功软化了恶性肿瘤，这种方法称之为纳米热疗法，其能够让肿瘤对治疗制剂更加敏感，相关研究刊登于... 详细信息

肿瘤的机械阻力和标准疗法的附带损伤往往会阻碍抵御癌症的效果，近日，来自法国国家健康与医学研究所等机构的研究人员通过对肿瘤加热成功软化了恶性肿瘤，这种方法称之为纳米热疗法，其能够让肿瘤对治疗制剂更加敏感，相关研究刊登于国际杂志Theranostics上。

关键词：恶性肿瘤热疗法软化纳米癌症疗效附带损伤机械阻力

Vibration analysis and Fatigue Assessment of *** II:Mechanical Equipment

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Journal of Civil Engineering and Architecture 2021年第10期15卷 504-510页

作者： ana C.S.da silva Gabriel M.Sant’Anna lucia H.G.Cardoso AndréV.Castilho JoséG.S.da silva State University of Rio de Janeiro(UERJ) Rio de Janeiro/RJ 20550-900Brazil Structural Engineering Department(ESTR) State University of Rio de Janeiro(UERJ)Rio de Janeiro/RJ 20550-900Brazil

Floors subjected to mechanical equipment loads frequently present problems associated with excessive vibration which can cause human discomfort or even reduce the structure service *** this context,this work aims to develop an analysis methodology in order to assess the fatigue performance of steel-concrete composite floors,when subjected to vibrations induced by mechanical *** studied structural model corresponds to a steel-concrete composite floor spanning 10 m by 10 m,with a total area of 100 m^(2).The numerical model developed for the dynamic analysis adopted the usual mesh refinement techniques present in finite element method(FEM)simulations implemented in the ANSYS *** investigated floor dynamic response was calculated through the consideration of the dynamic loadings imposed by the mechanical equipment,simulated based on the use of harmonic forces applied on the concrete ***,the dynamic structural response was performed considering several scenarios for the positioning of the equipment,in order to verify the occurrence of excessive *** fatigue assessment is based on a linear cumulative damage rule through the use of the Rainflow-counting algorithm and S-N curves from traditional design *** results of this investigation indicated that the equipment position affects directly the floor dynamic structural response and also significantly influences the structure service life.

关键词： Steel-concrete composite floors mechanical equipment dynamic structural analysis fatigue assessment.

Surface Finish Evaluation of AISI P100 Steel after Grinding with MQL Technique with Different Flow Rates

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Journal of Mechanics Engineering and Automation 2020年第2期10卷 60-65页

作者： Bruno Souza Abrão Mayara Fernanda pereira Cleudes Guimarães Lurian Souza Vieira da silva Rodrigo deSouza Ruzzi Rosemar Batista da silva Eduardo Carlos Bianchi Alisson Rocha Machado School of Mechanical Federal University of UberlandiaUberlandia/MGCEP 38.408-208Brazil School of Engineering São Paulo State UniversityBauru/SPCEP 17.033-360Brazil Mechanical Engineering Graduate Program Pontifícia Universidade Católica do Paraná(PUC-PR)R.Imaculada Conceição1155Bairro Prado VelhoCEP 80215-901Curitiba/PRBrazil

Grinding operation is a finishing process often employed when high precision and narrow geometric tolerances are *** requirements can be achieved only if cutting conditions are properly selected,especially the cooling-lubrication *** general,grinding is performed in presence of cutting fluid,however,due to the environmental impacts and costs of the conventional coolant delivery technique(flow rates from 4 L/min to 300 L/min),alternative cooling-lubrication techniques have been developed on restriction of the coolants *** the several techniques,MQL(minimum quantity of lubricant)technique has received special attention from machining users because of its advantages in terms of surface quality of workpiece and drastic reduction in use of *** this context,this paper evaluated the performance of the MQL technique as compared to the flood coolant in peripheral surface grinding of AISI P100(VP100)steel with conventional aluminum oxide grinding wheel in relation to the surface roughness(Ra and Rz).Input parameters tested were equivalent chip thickness(0.09μm,0.18μm and 0.27μm)and flow rate of the cutting fluid(60 mL/h,150 mL/h and 240 mL/h)of the MQL *** showed that the grinding with MQL technique provided lower surface roughness values compared to conventional flood cooling,especially when machining under the intermediary cutting ***,with exception of heq of 0.09 m,the MQL technique resulted in lower values of Rz parameter as compared to the conventional coolant technique,regardless of the flow rate tested.

关键词： Grinding AISI P100 steel MQL technique equivalent chip thickness surface roughness