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In Vitro Evaluation of Gamma Irradiation on a Gel Formulation of Cratylia Mollis: Rheological Proporties and Microbiological Control

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Journal of Cosmetics, Dermatological Sciences and Applications 2012年第2期2卷 45-50页

作者： Maria Helena Madruga Lima-Ribeiro Ralph Santos-Oliveira Mauricelia Firmino De Santana Terezinha De Jesus Andreoli Pinto Irene Satiko Kikuchi Cheila Goncalves Mothe Luana Cassandra Breitenbach Barroso Coelho Maria Tereza Dos Santos Correia Ana Maria Dos Anjos Carneiro-Leao Laboratory of Immunopathology Keizo Asami(LIKA) Federal University of PernambucoRecifeBrazil Nuclear Engineering Institute Laboratory of NanoradiopharmaceuticalsRio de JaneiroBrazil Biochemistry Department Federal University of PernambucoRecifeBrazil Pharmacy Department University of Sao PauloSao PauloBrazil School of Chemistry Federal University of Rio de JaneiroRio de JaneiroBrazil Departament of Morphology and Animal Physiology Federal Rural University of PernambucoRecifeBrazil

Lectin Cramoll-1,4, obtained from Cratylia mollis seeds (beans camaratu) was structurally characterized, biologically and pharmacologically, but its use as a biopharmaceutical is not well documented. The objective of this study is to propose a biopharmaceutical formulation lectin Cramoll-1,4, test their hemagglutinating properties in vitro as well as the use of gamma radiation as a continuous process of decontamination formulation. It was made of the extraction and purification Cramoll-1,4, was developed a gel formulation using Carbopol? as a vehicle, at concentrations of 50, 100, 200, 300 and 600 μg was irradiated with 60Co gamma rays in a dose of 7.549 kGy·h–1. The proposed formulation at a concentration of 300 μg produced an increase in the hemagglutinating units Cramoll-1,4 due to the synergistic effect caused by gamma radiation. Considering the diverse use of lectins, specific molecular and structural factors, as well as changes resulting from its formulation, concentration, irradiation and route of administration is of utmost importance to continue the studies in vitro, for subsequent application in vivo to characterize the physiological and molecular processes involved in the response and cellular effects.

关键词： Gamma Radiation Natural Products Hemagglutinating Activity Cramoll-1 4

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Phytochemical bioprospecting, antioxidant, antimicrobial and cytotoxicity activities of saline extract from Tithonia diversifolia(Hemsl) A.Gray leaves

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Asian Pacific Journal of Tropical Biomedicine 2018年第5期8卷 245-253页

作者： Bruno Rafael Barboza Bárbara Rafaela da Silva Barros Bárbara de Azevedo Ramos Maiara Celine de Moura Thiago Henrique Napoleao Maria Tereza dos Santos Correia Luana Cassandra Breitenbach Barroso Coelho Iranildo José da Cruz Filho Ana Maria Souto Maior Túlio Diego da Silva Leylianne de Cássia Rodrigues Nerys Edson Renan Barros de Santana Cláudia Sampaio de Andrade Lima Virgínia Maria Barros de Lorena Cristiane Moutinho Lagos de Melo Department of Antibiotics Biosciences Centre Federal University of Pernambuco Department of Biochemistry Biosciences Centre Federal University of Pernambuco Center for Strategic Techmologies of the Northeast Department of Biophysics Biosciences Centre Federal University of Pernambuco Aggeu Magalhaes Institute IAM/FIOCRUZ

Objective:To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting,and investigate its safety against animal cells.Methods:The saline extract was prepared,with NaCl(0.15 M),by constant stirring of the dried and pulverized leaves,followed by volume reduction by lyophilization.The extract was phytochemical characterized using ultra-performance liquid chromatography,and total phenol and flavonoid analysis also was performed.The antioxidant capacity was determined through DPPH*radical,the antimicrobial property was evaluated against standard bacteria and fungi,and the viability assays were performed against mice splenocytes.Results:Fifteen compounds were identified belonging to two main classes terpenoids and phenolics.The extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of flavonoid.Moreover,extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule[(3.042±0.019)mg AAE/g and(4.12±0.10)mg AAE/g to saline extract and butylated hydroxytoluene,respectively].The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes,in concentrations lower than 50μg/mL,promoting higher proliferation in these cells.Conclusions:Saline extract from Tithonia diversifolia leaves presents potential antioxidant,antifungal properties and induces immunostimulation in mice splenocytes.

关键词： Tithonia diversifolia Extract Antioxidant Antifungal properties Immunostimulation

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Stability of the Antioxidant Activity of Flavonoids after Gamma Irradiation

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Food and Nutrition Sciences 2011年第5期2卷 490-493页

作者： Antonio Fernando de Melo Vaz Marthyna Pessoa de Souza Adriana Carla Cavalcante Malta Argolo Ana Maria Mendonca de Albuquerque Melo Maria Luiza Vilela Oliva Haroldo Sátiro Xavier Luana Cassandra Breitenbach Barroso Coelho Maria Tereza dos Santos Correia Department of Biochemistry Federal University of PernambucoRecifeBrazil Department of Biophysics and Radiobiology Fed-eral University of PernambucoRecifeBrazil Department of Biochemistry Federal University of Sao PauloSao PauloBrazil Department of Pharmaceutical Science Federal University of PernambucoRecifeBrazil

Food irradiation has the purpose of destroying insects or microorganisms, thereby increasing the safety and shelf life of foods. Flavonoids are ubiquitous plant secondary products with radical scavenger ability. In the present study their antioxidant stability after gamma irradiation was evaluated. The flavonoids showed fast scavenger ability measured with the 2.2-diphenyl-1-picrylhydrazyl radical (DPPH) after high doses of radiation. The low damage caused by irra- diation on antioxidant capacity of the flavonoids shows their potential use in combating chemical oxidation of bio- molecules in irradiated foods.

关键词： Flavonoid Food Irradiation Scavenger Capacity Gamma Irradiation

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