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BnVIR:bridging the genotype-phenotype gap to accelerate mining of candidate variations underlying agronomic traits in Brassica napus

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Molecular Plant 2022年第5期15卷 779-782页

作者： Zhiquan Yang Congyuan Liang lulu wei Shengbo Wang Feifan Yin Dongxu Liu Liang Guo Yongming Zhou Qing-Yong Yang National Key Laboratory of Crop Genetic Improvement College of InformaticsHuazhong Agricultural UniversityWuhan 430070China Hubei Key Laboratory of Agricultural Bioinformatics College of InformaticsHuazhong Agricultural UniversityWuhan 430070China

Dear Editor,Genetic variations,which range from single nucleotide polymor-phisms(SNPs)and short insertion/deletions(InDels)to large-scale structural variations(SVs),are the basis of phenotypic diversity.Understanding the effect of genetic variations on traits is critical for uncovering the genetic architecture of various phe-notypes.

关键词： agronomic traits InDel

Editorial of Special Column“Novel Peptides and Peptidomimetics in Drug Discovery”

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Acta Pharmaceutica Sinica B 2021年第9期11卷 2606-2608页

作者： Jianfeng Cai lulu wei Department of Chemistry University of South Florida

Since insulin,a natural peptide composed of 51 amino acids,was first isolated and commercialized in the 1920s,peptide drugs have greatly reshaped our modern drug discovery area^(1).To date,the total number of approved peptide drugs for human use worldwide has exceeded 60^(2).Popular peptide drugs such as liraglutide(Victoza)^(2) and glucagon-like peptide 1(GLP-1)^(3) are the topselling drugs for type 2 diabetes.However,significant hurdles associated with natural peptide sequences as therapeutics,such as metabolic instability and,consequently,have low oral bioavailability and shorter half-life^(4),are still remaining.To overcome the limitations of natural peptides and further optimize their advantages,peptidomimetics emerged as an alternative strategy.

关键词： drugs Popular overcome

Purification and anti-inflammatory effect of selenium-containing protein fraction from selenium-enriched Spirulina platensis

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Food Bioscience 2022年第1期45卷 197-203页

作者： Pingyingzi Jiang Jinhao Meng Lifei Zhang Li Huang lulu wei Yunxia Bai Xiaoling Liu Shubo Li College of Light Industry and Food Engineering Guangxi UniversityNanning530004China

Spirulina platensis is an excellent carrier of Se,and thus widely used in medical fields and as well as in the food industry.However,there is little information about the characteristics and bioactivity of selenium-containing S.platensis proteins(Se-SP).In this study,Se-SP with different molecular weights were isolated from selenium-enriched S.platensis,and the bioactivities(such as antioxidant and anti-inflammatory activities)of Se-SP were investigated.Se-SP3(with a molecular weight range of 20-48 kDa)showed better free radical scavenging ability(ABTS)than the other Se-SPs.In addition,Se-SP3 suppressed inflammatory cytokines,in which decreased by 74% interleukin 6(IL-6),42.28% tumor necrosis factor-α(TNF-α),69.07% content of malondialdehyde(MDA),40.45% interleukin-1β(IL-1β)relative to the LPS group.Moreover,Se-SP3 decreased the nitric oxide(NO)production by 64.84% compared with the LPS group and increased the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px),indicating that Se-SP3 has excellent antioxidant and anti-inflammatory activities,and could be used as a functional food ingredient or natural medicine.

关键词： Selenium Spirulina platensis protein Purification Bioactivities Inflammatory cytokines

An aqueous ZnCl2/Fe(bpy)3Cl2 flow battery with mild electrolyte

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Frontiers of Materials Science 2020年第4期14卷 442-449页

作者： Zhipeng XIE lulu wei Shengwen ZHONG Faculty of Materials Metallurgy and Chemitry Jiangxi University of Science and TechnologyGanzhou 341000China

The kinetics of electrode reaction was investigated by cyclic voltammetry,and cyclic voltammograms show that the reversibility of the Fe(bpy)3^2+/Fe(bpy)^3+electrode reaction is better than that of the Zn/Zn^2+electrode reaction on the graphite disc.However,the Fe(bpy)3^2+ion diffusion in electrolyte is subject to greater resistance than that of the Zn^2+ion one.The stability of the Fe(bpy)3Cl2 solution was investigated by UV-vis spectroscopy,and the performance of a mild redox flow battery employing ZnCl2 and Fe(bpy)3Cl2 in the NaCl aqueous solution with various membranes as the separator was also investigated.It was found that the Celgard 3501 membrane cannot effectively prevent Fe(bpy)3^2+ions from leaking into anolyte,leading to the rapid failure of the flow battery.Although the Nafion 115 membrane can be polluted by Fe(bpy)32+ions,it is not invalidated.The Nafion 115 membrane shows good selectivity,which can avoid Fe(bpy)32 ions from leakage into anolyte.The ZnCl2/Fe(bpy)3Cl2 flow battery with the Nafion 115 membrane exhibits the capacity retention of 80%after 200 cycles.

关键词： redox flow battery zinc bipyridine iron

星型-三角形负泊松比结构的面内压缩刚度可调控特性

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振动与冲击 2024年第4期43卷 105-114页

作者：魏路路许世维朱国华赵轩西安建筑科技大学机电工程学院西安710055 长安大学汽车运输安全保障技术交通行业重点实验室西安710064

通过准静态面内压缩试验揭示星型-三角形结构(star-triangular honeycomb, STH)的刚度可调控特性。利用有限元模拟的方法研究非均匀壁厚STH的面内压缩刚度可调控机制,分析不同类型胞壁厚度比(t_(1)=t_(3))/t_(2)及胞壁角对其面内压缩刚... 详细信息

通过准静态面内压缩试验揭示星型-三角形结构(star-triangular honeycomb, STH)的刚度可调控特性。利用有限元模拟的方法研究非均匀壁厚STH的面内压缩刚度可调控机制,分析不同类型胞壁厚度比(t_(1)=t_(3))/t_(2)及胞壁角对其面内压缩刚度的影响。结果表明,STH在面内压缩载荷下呈2个线弹性阶段,且强化刚度比初始刚度显著增大。适当地增大内凹胞壁和垂直胞壁的厚度可同时提高STH的初始刚度及强化刚度;当(t_(1)=t_(3))/t_(2)>1.575后,初始刚度仍持续增大,而强化刚度反而逐渐减小,最终不再具有强化刚度阶段。胞壁角α≤30°时,增大胞壁角可提高STH的初始刚度及强化刚度,反之,初始刚度及强化刚度对应的应变随胞壁角的增大逐渐增大,而强化刚度逐渐减小并趋于消失。该研究将为可调节刚度负泊松比结构的设计提供新的思路。

关键词：星型-三角形结构(STH) 负泊松比可调控刚度非均匀壁厚准静态压缩

α/Sulfono-γ-AA peptide hybrids agonist of GLP-1R with prolonged action both in vitro and in vivo

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Acta Pharmaceutica Sinica B 2023年第4期13卷 1648-1659页

作者： Yan Shi Candy Lee Peng Sang Zaid Amso David Huang weixia Zhong Meng Gu lulu wei Vân T.B.Nguyen-Tran Jingyao Zhang weijun Shen Jianfeng Cai Department of Chemistry University of South FloridaTampaFL 33620USA Calibr at Scripps Research La JollaCA 92037USA

Peptides are increasingly important resources for biological and therapeutic development,however,their intrinsic susceptibility to proteolytic degradation represents a big hurdle.As a natural agonist for GLP-1R,glucagon-like peptide 1(GLP-1)is of significant clinical interest for the treatment of type-2 diabetes mellitus,but its in vivo instability and short half-life have largely prevented its therapeutic application.Here,we describe the rational design of a series of a/sulfono-γ-AA peptide hybrid analogues of GLP-1 as the GLP-1R agonists.Certain GLP-1 hybrid analogues exhibited enhanced stability(t_(1/2)>14 days)compared to t_(1/2)(<1 day)of GLP-1 in the blood plasma and in vivo.These newly developed peptide hybrids may be viable alternative of semaglutide for type-2 diabetes treatment.Additionally,our findings suggest that sulfono-γ-AA residues could be adopted to substitute canonical amino acids residues to improve the pharmacological activity of peptide-based drugs.

关键词： GLP-1 Peptidomimetics Helical structures Stability Type-2 diabetes treatments Rational design GLP-1R agonists Pharmacological activity

CSN6 promotes tumorigenesis of gastric cancer by ubiquitin-independent proteasomal degradation of p16INK4a

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Cancer Biology & Medicine 2019年第3期16卷 514-529页

作者： Wenqi Du Zongxiang Liu Wentao Zhu Tongtong Li Zhiman Zhu lulu wei Jun Song Dongsheng Pei Department of Pathology Xuzhou Medical UniversityXuzhou221004China Department of Anatomy Xuzhou MedicalUniversityXuzhou221004China Department of Periodontal Mucosal Diseases the Affiliated Xuzhou StomatologicalHospital of Xuzhou Medical UniversityXuzhou221002China Department of General Surgery the Affiliated Hospital ofXuzhou Medical UniversityXuzhou221002China Institute of Digestive Diseases Xuzhou Medical UniversityXuzhou221002China

Objective: CSN6 is a vital subunit of the constitutive photomorphogenesis 9(COP9) signalosome(CSN), which is responsible for development disorders and promotes ubiquitin-26 S proteasome-dependent degradation in vitro and vivo.Its role in the tumor development of gastric cancer remains unclear.In this study, we investigated the role of CSN6 in gastric cancer progression.Methods: Human gastric cancer samples were collected and immunohistochemistry was performed to identify the role of CSN6 in gastric cancer.The cell proliferation was measured by CCK-8 and the EdU incorporation method.Immunofluorescence localization and a co-immunoprecipitation study were used to show the interaction between the protein CSN6 and p16.Ubiquitination assay was performed to validate whether ubiquitination is involved in CSN6-mediated p16 degradation.BALB/c nude mice were used to produce a tumor model in order to test the effect of CSN6 on cancer growth in vivo.Results: CSN6 expression was dramatically increased in gastric cancer tissues compared with paired adjacent non-tumor tissues and CSN6 was correlated with worse overall and disease-specific survival.Additionally, we also found that CSN6 downregulated p16 protein expression, thereby promoting gastric cancer cell growth and proliferation.Moreover, CSN6 interacted with p16 and a proteasome activator REGγ(PA28γ), thereby facilitating ubiquitin-independent degradation of p16.Conclusions: CSN6 promoted the loss of p16-mediated tumor progression and played an important role in regulating ubiquitin-independent proteasomal degradation of p16.

关键词： CSN6 gastric cancer proliferation p16 REGγ

One-step synthesis and self-assembly behavior of thermo-responsive star-shaped β-cyclodextrin-（P（M EO2MA-co-PEGMA））21 copolymers

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Frontiers of Materials Science 2017年第3期11卷 223-232页

作者： lulu wei Beibei LU Lei LI Jianning WU Zhiyong LIU Xuhong GUO School of Chemistry and Chemical Engineering Shihezi University/Key Laboratory of Materials-Oriented Chemical Engineering of Xinjiang Uygur Autonomous Region/Engineering Research Center of Materials-Oriented Chemical Engineering of Xinjiang Bingtuan Shihezi 832003 China State Key Laboratory of Chemical Engineering East China University of Science and Technology Shanghai 200237 China

A novel β-cyclodextrin-poly（2-（2-methoxyethoxy）ethyl methacrylate）-co- poly（ethylene glycol） methacrylate （abbreviated as： β-CD-（P（MEO2MA-co-PEGMA））21） was prepared by using the one-step strategy, and then the star-shaped copolymers were used in the atom transfer radical polymerization （ATRP）. The structure of star-shaped β- CD-（P（MEO2MA-co-PEGMA））21 copolymers were studied by FTIR, 1H NMR and gel permeation chromatography （GPC）. The star-shaped copolymers could self-assembled into micelles in aqueous solution owing to the outer amphiphilic β-CD as a core and the hydrophilic P（MEO2MA-co-PEGMA） segments as a shell. These thermo-responsive starshaped copolymers micelles exhibited lower critical solution temperature （LCST） in water, which could be finely tuned by changing the feed ratio of MEO2MA to PEGMA. The LCST of star-shaped β-CD-（P（MEO2MA-co-PEGMA））21 copolymer micelles were increased from 35℃ to 58℃ with the increasing content of PEGMA. The results were investigated by DLS and TEM. When the temperature was higher than corresponding LCSTs, the micelles started to associate and form spherical nanoparticles. Therefore, β- CD-（P（MEO2MA-co-PEGMA））21 star-shaped copolymer micelles could be potentially applied in nano-carrier, nano-reactor, smart materials and biomedical fields.

关键词： star-shaped copolymers thermo-responsive β-cyclodextrin （β-CD） self- assembly atom transfer radical polymerization （ATRP）

Peptidomimetic-based antibody surrogate for HER2

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Acta Pharmaceutica Sinica B 2021年第9期11卷 2645-2654页

作者： Mengmeng Zheng Chunpu Li Mi Zhou Ru Jia Fengyu She lulu wei Feng Cheng Qi Li Jianfeng Cai Yan Wang Department of Chemistry University of South FloridaTampaFL 33620USA Department of Medical Oncology&Cancer Institute of Medicine Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai 201203China Academy of Integrative Medicine Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmaceutical Science College of PharmacyUniversity of South FloridaTampaFL 33612USA

Inhibition of human epidermal growth factor receptor 2 mediated cell signaling pathway is an important therapeutic strategy for HER2-positive cancers.Although monoclonal antibodies are currently used as marketed drugs,their large molecular weight,high cost of production and susceptibility to proteolysis could be a hurdle for long-term application.In this study,we reported a strategy for the development of artificial antibody based on y-AApeptides to target HER2 extracellular domain(ECD).To achieve this,we synthesized a one-bead-two-compound(OBTC)library containing 320,000 cyclic y-AApeptides,from which we identified a y-AApeptide,M-3-6,that tightly binds to HER2 selectively.Subsequently,we designed an antibody-like dimer of M-3-6,named M-3-6-D,which showed excellent binding affinity toward HER2 comparable to monoclonal antibodies.Intriguingly,M-3-6-D was completely resistant toward enzymatic degradation.In addition,it could effectively inhibit the phosphorylation of HER2,as well as the downstream signaling pathways of AKT and ERK.Furthermore,M-3-6-D also efficiently inhibited cell proliferation in vitro,and suppressed tumor growth in SKBR3 xenograft model in vivo,implying its therapeutic potential for the treatment of cancers.Its small molecular weight,antibody-like property,resistance to proteolysis,may enable it a new generation of artificial antibody surrogate.Furthermore,our strategy of artificial antibody surrogate based on dimers of cyclicγ-AApeptides could be applied to a myriad of disease-related receptor targets in future.

关键词： HER2 Anti-cancer Peptidomimetics γ-AApeptides Antibody-surrogate