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Salvianolic acid B protects hepatocytes from H2O2 injury by stabilizing the lysosomal membrane

World Journal of Gastroenterology 2017年第29期23卷 5333-5344页

作者： Xiao-Feng Yan Pei Zhao Dong-Yan Ma Yi-lu Jiang Jiao-Jiao luo Liu Liu Xiao-Ling Wang Department of Biology School of Basic Medical Science Shanghai University of Traditional Chinese Medicine Women and Infant Hospital of Zhengzhou Shanghai university of Traditional Chinese Medicine

AIM To investigate the capability of salvianolic acid B(Sal B) to protect hepatocytes from hydrogen peroxide(H_2O_2)/carbon tetrachloride(CCl_4)-induced lysosomal membrane permeabilization. MeTHODS Cell Counting Kit-8 assay was used to measure cell viability. Apoptosis and death were assayed through flow cytometry. Brd u incorporation was used to detect cell proliferation. Serum alanine aminotransferase activity and liver malondialdehyde(MDA) content were measured. Liver histopathological changes were evaluated using hematoxylin-eosin staining. Lysosomal membrane permeability was detected with Lyso Tracker Green-labeled probes and acridine orange staining. The levels of protein carbonyl content(PCC), cathepsins(Cat)B/D, and lysosome-associated membrane protein 1(LAMP1) were evaluated through western blotting. Cytosol Cat B activity analysis was performed with chemiluminescence detection. The m RNA level ofLAMP1 was evaluated through quantitative real-time polymerase chain reaction. ReSuLTS Results indicated that H_2O_2 induced cell injury/death. Sal B attenuated H_2O_2-induced cell apoptosis and death, restored the inhibition of proliferation, decreased the amount of PCC, and stabilized the lysosome membrane by increasing the LAMP1 protein level and antagonizing Cat B/D leakage into the cytosol. CCl_4 also triggered hepatocyte death. Furthermore, Sal B effectively rescued hepatocytes by increasing LAMP1 expression and by reducing lysosomal enzyme translocation to the *** Sal B protected mouse embryonic hepatocytes from H_2O_2/CCl_4-induced injury/death by stabilizing the lysosomal membrane.

关键词： Lysosomal membrane permeabilization Injury Salvianolic acid B Hepatocyte

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Sephadex G10凝胶色谱法测定磺苄西林钠中的高分子杂质

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华西药学杂志 2007年第6期22卷 699-700页

作者：田贵容吕露阳方梅四川大学华西药学院四川成都610041 成都英创科技发展有限责任公司四川成都610041

目的建立测定磺苄西林钠中高分子杂质的方法。方法采用高效液相色谱法,色谱柱为Sephadex G10凝胶柱,流动相A为0.05 mol.L-1的磷酸盐缓冲液(pH7.0)、B为高纯水,流速为1 ***-1,检测波长254 nm,柱温为25℃。结果磺苄西林钠中高分子杂质含... 详细信息

目的建立测定磺苄西林钠中高分子杂质的方法。方法采用高效液相色谱法,色谱柱为Sephadex G10凝胶柱,流动相A为0.05 mol.L-1的磷酸盐缓冲液(pH7.0)、B为高纯水,流速为1 ***-1,检测波长254 nm,柱温为25℃。结果磺苄西林钠中高分子杂质含量小于0.12%。结论所建方法灵敏、准确、简便,可用于磺苄西林钠中高分子杂质的质量控制。

关键词：葡萄糖凝胶G10 磺苄西林钠高分子杂质

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5种干预疗法对去卵巢骨质疏松大鼠骨髓脂肪细胞数目和PPARγ2蛋白表达水平的影响

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首都体育学院学报 2016年第3期28卷 270-273页

作者：卜淑敏杨涵首都体育学院运动科学与健康学院北京100191 成都体育学院成都610041

采用去卵巢骨质疏松大鼠模型,观察跑台运动、全身垂直振动、金雀异黄酮、雌激素和氯化锂5种干预疗法对去卵巢骨质疏松大鼠骨髓脂肪细胞数目和过氧化物酶体增殖激活物受体γ2(PPARγ2)蛋白表达水平的影响。方法:将80只3月龄雌性SD大鼠按... 详细信息

采用去卵巢骨质疏松大鼠模型,观察跑台运动、全身垂直振动、金雀异黄酮、雌激素和氯化锂5种干预疗法对去卵巢骨质疏松大鼠骨髓脂肪细胞数目和过氧化物酶体增殖激活物受体γ2(PPARγ2)蛋白表达水平的影响。方法:将80只3月龄雌性SD大鼠按体重分层后随机分为假手术组和去卵巢组,去卵巢10周后,将去卵巢组大鼠按体重分层后又随机分为去卵巢组、雌激素组、金雀异黄酮组、氯化锂组、跑台运动组和全身垂直振动组。去卵巢第11周时,不同组别的大鼠开始进行不同干预处理。干预8周后,用He染色法观察左胫骨组织形态学和骨髓脂肪细胞空泡数目的变化;用Western blotting检测右胫骨近端骨组织PPARγ2蛋白表达水平的变化。结果:经雌激素、金雀异黄酮、跑台运动和氯化锂干预后,去卵巢骨质疏松大鼠胫骨松质骨骨小梁数目显著增加,脂肪空泡数目和PPARγ2蛋白表达水平显著下降,而经全身垂直振动干预后,去卵巢骨质疏松大鼠胫骨松质骨骨小梁数目显著增加,脂肪空泡数量显著减少,但PPARγ2蛋白表达水平无显著变化。结论:雌激素、金雀异黄酮、跑台运动和氯化锂可能是通过下调去卵巢骨质疏松大鼠骨组织PPARγ2蛋白的表达水平而抑制去卵巢骨质疏松大鼠骨髓细胞向脂肪细胞的分化。

关键词：去卵巢大鼠跑台运动全身垂直振动金雀异黄酮 PPARγ2

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女运动员心肌收缩力信号探测方法和部位的研究

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体育科学 2008年第9期28卷 55-60页

作者：步斌王爱华成都体育学院运动医学系四川成都610041

目的:应用运动心力监测仪和运动试验的方法检测女运动员的心力变化趋势(CCCT)和第一心音(S1)幅值,对比研究心前区不同位置,胸骨体中下部(T区)和心尖部(M区))心力信号的差异性和相关性,以确定女运动员心音传感器放置的较佳位置;为女运动... 详细信息

目的:应用运动心力监测仪和运动试验的方法检测女运动员的心力变化趋势(CCCT)和第一心音(S1)幅值,对比研究心前区不同位置,胸骨体中下部(T区)和心尖部(M区))心力信号的差异性和相关性,以确定女运动员心音传感器放置的较佳位置;为女运动员心脏机能评定和临床康复女病人心功能评定提供新的思路;方法:随机纳入受试者60人,分为女运动员组(FA组),普通女大学生组(FC组),男运动员组(MA组)及普通男大学生组(MC组)。受试者在功率自行车上做递增负荷实验直至疲劳,应用运动心力监测仪记录受试者在运动的每个阶段的S1幅值,通过对每个受试者在完成不同运动的过程中胸骨体中下部S1幅值和心尖部S1幅值的监测,分别比较在不同运动负荷下女性受试者的胸骨体中下部S1幅值和心尖部S1幅值,男性受试者胸骨体中下部S1幅值和心尖部S1幅值;比较分析FA组与FC组,MA组与MC组在不同负荷下的心力变化趋势(CCCT),以及不同位置间的CCCT和S1之间的关系。结果:1)胸骨体中下部S1幅值与心尖部S1幅值的比较,在安静时及运动停止后5minFA组与FC组,MA组与MC组之间均存在非常显著性差异;胸骨体中下部S1幅值比心尖部S1幅值高2倍左右,且信号清晰,噪音干扰较少;2)胸骨体中下部S1幅值/心尖部S1幅值的相对比值,运动员与普通大学生之间不存在显著性差异;3)胸骨体中下部S1幅值和心尖部S1幅值呈正相关,其相关系数女性受试者为r=0.925(P<0.01),男性受试者为r=0.871(P<0.01);4)各组不同位置之间的CCCT数据均不存在显著性差异。结论:1)男女性同一受试者,同一时间段内,不同位置间的S1幅值比较存在显著性差异。胸骨体中下部采集的S1幅值较高,大约是在心尖部采集的S1幅值的2倍左右,且信号清晰,噪音干扰较少;2)心前区S1幅值和胸骨体中下部S1幅值呈高度正相关,因而心前区胸骨体下端可以作为今后女运动员心力信号采集的一个常规部位;3)两位置的CCCT数据间不存在显著性差异,这表明用CCCT这一相对值检测心肌收缩力可不受部位,胸壁厚薄等因素的影响,是一项较为可靠的心力检测指标。

关键词：女子运动员心肌收缩力心脏听诊区心力变化趋势

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350t倾动炉筑炉技术

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有色设备 2004年第Z1期18卷 55-57,63页

作者：鲁兴华郑步东江西铜业集团公司贵溪冶炼厂江西贵溪335424

综述我国第一台倾动炉的结构特点、耐火材料应用情况及筑炉技术,为我国建设第二台倾动炉提供参考。

关键词：倾动炉含铜物料镁铬砖筑炉技术

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Low-loading gold in situ doped with sulfur by biomolecule-assisted approach for promoted electrochemical carbon dioxide reduction

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Nano Research 2023年第2期16卷 2059-2064页

作者： Meijie Tan Xiaoqian Han Sen Ru Chao Zhang Zhouru Ji Zhaolin Shi Guomeng Qiao Yunying Wang Ruixue Cui Qiquan luo Jiqing Jiao Yaguang Li Tongbu lu MOe International Joint Laboratory of Materials Microstructure Institute for New Energy Materials and Low Carbon TechnologiesSchool of Materials Science and EngineeringTianjin University of TechnologyTianjin 300384China Institutes of Physical Science and Information Technology Anhui UniversityHefei 230601China Haihe Laboratory of Sustainable Chemical Transformations Tianjin 300192China College of Materials Science and engineering State Key Laboratory of Bio-Fibers and Eco-TextilesQingdao UniversityQingdao 266071China Hebei Key Lab of Optic-electronic Information and Materials College of Physics Science and TechnologyInstitute of Life Science and Green DevelopmentHebei UniversityBaoding 071002China

For electrochemical carbon dioxide reduction(CO_(2)RR),CO_(2)-to-CO conversion is considered an ideal route towards carbon neutrality for practical ***(Au)is known as a promising catalyst with high selectivity for CO;however,it suffers from high cost and low mass-specific *** this study,we design and prepare a catalyst featuring uniform S-doped Au nanoparticles on N-doped carbon support(denoted as S-Au/NC)by an in situ synthesis strategy using *** S-Au/NC displays high activity and selectivity for CO in CO_(2)RR with a Au loading as low as 0.4 wt.%.The Faradaic efficiency of CO(FeCO)for S-Au/NC is above 95%at−0.75 V(***);by contrast,the FeCO of Au/NC(without S)is only 58%.The Tafel slope is 77.4 mV·dec−1,revealing a favorable kinetics ***,S-Au/NC exhibits an excellent long-term stability for CO_(2)*** functional theory(DFT)calculations reveal that the S dopant can boost the activity by reducing the free energy change of the potential-limiting step(formation of the*COOH intermediate).This work not only demonstrates a model catalyst featuring significantly reduced use of noble metals,but also establishes an in situ synthesis strategy for preparing high-performance catalysts.

关键词： electrochemical CO_(2)reduction nanocomposites heterogeneous catalyst in situ sulfured heteroatoms

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Blast Pressure Measurements of an underwater Detonation in the Sea

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Journal of Marine Science and Application 2021年第4期20卷 706-713页

作者： Alpaslan Tatlısuluoğlu Serdar Beji Ph.D.student at I.T.u.and underwater Search and RescueCommand TR-34469 IstanbulTurkey Faculty of Naval Architecture and Ocean engineering Istanbul Technical UniversityTR-34469 IstanbulTurkey

Blast pressure measurements of a controlled underwater explosion in the sea were carried *** explosive of 25-kg trinitro-toluene(TNT)equivalent was detonated,and the blast pressures were recorded by eight diferent high-performance pressure sensors that work at the nonresonant high-voltage output in adverse underwater *** peak pressure values are used to establish a relationship in the well-known form of empirical underwater explosion(uNDeX)loading *** of the formula are redetermined by employing the least-squares method in two diferent forms for best ftting to the measured *** newly determined constants are found to be only slightly diferent from the generally accepted ones.

关键词： underwater explosions High-pressure shock waves efects of directionality on blast pressure records Pressure loading formulas Applications of the least-squares method

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Nutritional, Microbiological and Sensory Characteristics of Malted Soy-Kunu Zaki: An Improved Traditional Beverage

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Advances in Microbiology 2013年第4期3卷 389-397页

作者： Aminat O. Adelekan Adediran e. Alamu Ngozi u. Arisa Yetunde O. Adebayo Abidemi S. Dosa Department of Food Science and Technology Bells University of Technology Ota Nigeria

The nutritional, microbiological and sensory characteristics of improved kunu-zaki produced from malted cereals enriched with malted soymilk at different substitution levels (0% - 30%) were investigated. enrichment with soymilk and malting increased the protein, amino acid, ash and moisture content of the improved kunu-zaki. Malting resulted in a decrease in carbohydrate and fat content. The protein content of the malted samples ranged between 2.79% and 3.82% while that of unmalted was 2.36%. There was decrease in the concentration of phytic acid and trypsin inhibitor after malting but the concentration increased as soymilk was added. Microbiological analyses showed that microbial load of samples increased with the addition of soymilk. There was a significant difference (p 0.05) in the sensory attributes, acceptability decrease with increase in soymilk addition because of the beany flavor.

关键词： Nutritional Microbiological Malting Sensory Kunu-Zaki

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Two Innovative Techniques to Study the environmental Impacts of urban Forms

Two Innovative Techniques to Study the Environmental Impacts...

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第四届粤港澳可持续发展研讨会

作者： u Wa TANG Department of Civil and environmental engineering university of Macau

This paper introduces two innovative techniques,i.e.,the vehicle-following measuring technique and the high-spatial resolution air/noise modeling technique,that have been used recently to study of urban environment in the Macao *** related interna- tional conference papers have been awarded as one of the best student papers in the annual din- ner of the Hong Kong Society for Transportation Studies in 2007 and the annual dinner of the International Association of Chinese Professionals in Geographic Information Sciences in 2008. By using the vehicle-following measuring technique,vehicle gaseous emissions(NO, CO,CO and HC)and driver’s particle exposures(PM1,PM2.5,and PM10)have been measured during heavy traffic congestion in urban *** with the traditional la- boratory tests,the vehicle-following measurement technique can obtain a wide variety of vehi- cle emission data on the road as cooperation from the vehicle’s owners is no longer necessary. By using the high-spatial resolution modeling technique,traffic-induced air and noise pollu- tions in front of individual building facades on both sides of the road can be *** two innovative techniques have the capability to provide city decision-makers with a different perspective on vehicle transport and urban form that both impact air quality and noise.

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碳纳米管压阻效应及其应用研究

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功能材料 2004年第Z1期35卷 2881-2883页

作者：王万录廖克俊吕建伟万步勇张毅重庆大学应用物理系重庆400044 重庆大学应用物理系重庆400044 重庆大学应用物理系重庆400044 重庆大学应用物理系重庆400044 重庆大学应用物理系重庆400044

利用了三点弯曲法研究了碳纳米管膜压阻效应.研究中所用的碳纳米管是用化学气相沉积法合成的.实验结果表明,碘掺杂和未掺杂的碳纳米管膜的压阻因子在500微形变和室温下分别为350和65,超过了单晶硅和多晶硅的压阻因子.同时还发现化学处... 详细信息

利用了三点弯曲法研究了碳纳米管膜压阻效应.研究中所用的碳纳米管是用化学气相沉积法合成的.实验结果表明,碘掺杂和未掺杂的碳纳米管膜的压阻因子在500微形变和室温下分别为350和65,超过了单晶硅和多晶硅的压阻因子.同时还发现化学处理还能进一步增强碳纳米管膜的压阻效应.碘掺杂碳纳米管膜的压阻效应也许是由于在形变时带隙变化所致,而未掺杂碳纳米管压阻效应主要是由管之间接触电阻变化引起的.碳纳米管压阻效应可用于旋转速度的测量.

关键词：压阻效应碳纳米管碘掺杂形变诱导电阻变化

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