检索结果-南通市图书馆

Study of Serum Metabonomics and Formula-Pattern Correspondence in Coronary Heart Disease Patients Diagnosed as Phlegm or Blood Stasis Pattern Based on Ultra Performance Liquid Chromatography Mass Spectrometry

引用

Chinese Journal of Integrative Medicine 2018年第12期24卷 905-911页

作者： lu xiao-yan XU Hao ZHAO Tie LI Geng Department of Integrative Cardiology China-Japan Friendship Hospital Cardiovascular Diseases Center Xiyuan HospitalChina Academy of Chinese Medical Sciences

Objectives： To study the characteristics of serum metabonomics in coronary heart disease （CHD） patients diagnosed as phlegm or blood stasis pattern and explore effects of formula-pattern correspondence treatment. Methods： A total of 102 stable CHD patients were enrolled and divided into phlegm group （P group, n=52） and blood stasis group （BS group, n=50） according to pattern identification. Gualou Xiebai Banxia Decoction （瓜萎薤白半夏汤, GXBD） and Xuefu Zhuyu Decoction （血府逐瘀汤, XZD） were used as drug interventions. Relevant indicators of metabonomics were observed by ultra performance liquid chromatography mass spectrometry （UPLC-MS） and pattern recognition. Results： Levels of amino acids and phosphatidylethanolamine （PE） in the CHD group were much higher than those in healthy control group, while the levels of unsaturated fatty acids, sphingosine, Lyso, phosphatidylcholine （PC） were significantly lower （P〈0.01）. Most of the differential metabolites between the CHD and the healthy groups were also common metabolites of phlegm and blood stasis. 7（Z）, 10（Z）- hexadecadienoic acid and DPA were decreased in the P group and increased in the BS group. According to the quantity of retraced metabolites, improvement in metabonomics by formula-pattern correspondence was superior to that without correspondence in the BS group. Based on the varieties of metabolites, GXBD could improve the levels of docosapentaenoic acid （DPA）, sphingomyelin （SM）（d34：1）, and L-Lactic acid and XZD could ameliorate the levels of sphingosine and Vit E in the P group, in the BS group, GXBD could improve vitamin E level and XZD could make improvements in the levels of octadecanoic acid, phosphoglycerol, and SM （d34：1）. Conclusions： Phlegm and blood stasis in CHD patients present specific differential metabolites, and share common metabolites. Remarkable differences have been displayed in pathological properties and severity of phlegm and blood stasis. Patients with phlegm are more likely to have lipid metabo

关键词： ultra performance liquid chromatography mass spectrometry metabonomics phlegm/stasis pattern in coronary heart disease disease-pattern combination formula-pattern correspondence

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Danshen-Chuanxiongqin Injection attenuates cerebral ischemic stroke by inhibiting neuroinflammation via the TLR2/ TLR4-MyD88-NF-κB Pathway in tMCAO mice

引用

Chinese Journal of Natural Medicines 2021年第10期19卷 772-783页

作者： XU xiao-Jing LONG Jin-Bo JIN Kai-Yu CHEN Li-Bing lu xiao-yan FAN xiao-Hui Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China State Key Laboratory of Component-Based Chinese Medicine Tianjin University of Traditional Chinese MedicineTianjin 301617China

Danshen-Chuanxiongqin Injection(DCI)is a commonly used traditional Chinese medicine for the treatment of cerebral ischemic stroke in ***,its underlying mechanisms remain completely *** current study was designed to explore the protective mechanisms of DCI against cerebral ischemic stroke through integrating whole-transcriptome sequencing coupled with network pharmacology ***,using a mouse model of cerebral ischemic stroke by transient middle cerebral artery occlusion(tMCAO),we found that DCI(4.10 mL·kg−1)significantly alleviated cerebral ischemic infarction,neurological deficits,and the pathological injury of hippocampal and cortical neurons in ***,the whole-transcriptome sequencing was performed on brain *** cerebral ischemia disease(CID)network was constructed by integrating transcriptome sequencing data and cerebrovascular disease-related *** results showed CID network was imbalanced due to tMCAO,but a recovery regulation was observed after DCI *** analysis of the key genes with recovery efficiency showed that the neuroinflammation signaling pathway was highly enriched,while the TLR2/TLR4-MyD88-NF-κB pathway was predicted to be ***,the in vivo validation experiments confirmed that DCI exhibited protective effects against cerebral ischemic stroke by inhibiting neuroinflammation via the TLR2/TLR4-MyD88-NF-κB *** interestingly,DCI markedly suppressed the neutrophils infiltrated into the brain parenchyma via the choroid plexus route and showed anti-neuroinflammation *** conclusion,our results provide dependable evidence that inhibiting neuroinflammation via the TLR2/TLR4-MyD88-NF-κB pathway is the main mechanism of DCI against cerebral ischemic stroke in mice.

关键词： Danshen-Chuanxiongqin Injection Cerebral ischemic stroke Network pharmacology Neuroinflammation Neutrophil Choroid plexus

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Potential hepatic and renal toxicity induced by the biflavonoids from Ginkgo biloba

引用

Chinese Journal of Natural Medicines 2019年第9期17卷 672-681页

作者： LI Yun-Ying lu xiao-yan SUN Jia-Li WANG Qing-Qing ZHANG Yao-Dan ZHANG Jian-Bing FAN xiao-Hui Tianjin University of Traditional Chinese Medicine Tianjin 301617China Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Zhejiang University-Wanbangde Pharmaceutical Group Joint Research Center for Chinese Medicine Modernization Hangzhou 310058China

Evidence continues to grow on potential health risks associated with Ginkgo biloba and its *** biflavonoid is a subclass of the flavonoid family in Ginkgo biloba with a plenty of pharmacological properties,the potential toxicological effects of biflavonoids remains largely ***,the aim of this study was to investigate the in vitro and in vivo toxicological effects of the biflavonoids from Ginkgo biloba(i.e.,amentoflavone,sciadopitysin,ginkgetin,isoginkgetin,and bilobetin).In the in vitro cytotoxicity test,the five biflavonoids all reduced cell viability in a dose-dependent manner in human renal tubular epithelial cells(HK-2)and human normal hepatocytes(L-02),indicating they might have potential liver and kidney *** the in vivo experiments,after intragastrical administration of these biflavonoids at 20 mg·kg^–1·d^–1 for 7 days,serum biochemical analysis and histopathological examinations were *** activity of alkaline phosphatase was significantly increased after all the bifl avonoid administrations and widespread hydropic degeneration of hepatocytes was observed in ginkgetin or b ilobetin-treated ***,the five biflavonoids all induced acute kidney injury in treated mice and the main pathological lesions were confirmed to the tubule,glomeruli,and interstitium *** the in vitro and in vivo results suggested that these biflavonoids may be more toxic to the kidney than the liver,we further detected the mechanism of biflavonoids-induced *** increased TUNEL-positive cells were detected in kidney tissues of biflavonoids-treated mice,accompanied by elevated expression of proapoptotic protein BAX and unchanged levels of antiapoptotic protein BCL-2,indicating apoptosis was involved in biflavonoids-induced *** together,our results suggested that the five biflavonoids from Ginkgo biloba may have potential hepatic and renal toxicity and more attentions should be paid to ensure Ginkgo biloba p

关键词： Biflavonoids Ginkgo biloba Potential toxicity Liver Kidney Apoptosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于对数正态分布第k试验寿命的分散系数法

引用

航空动力学报 2006年第4期21卷 738-740页

作者：卢小艳陆山西北工业大学动力与能源学院西安710072

针对发动机构件寿命试验评估中对寿命分散系数计算公式与不同取值的需求,本文导出了寿命符合对数正态分布、基于任意第k试验寿命分散系数计算公式。并给出置信度为0.95、可靠度为99.87%、母体对数正态标准差lσgN=0.13时的寿命分散系数... 详细信息

针对发动机构件寿命试验评估中对寿命分散系数计算公式与不同取值的需求,本文导出了寿命符合对数正态分布、基于任意第k试验寿命分散系数计算公式。并给出置信度为0.95、可靠度为99.87%、母体对数正态标准差lσgN=0.13时的寿命分散系数。本文基于最差试件寿命的分散系数与已有文献一致,而基于最好试件寿命分散系数较已有文献更为合理;采用本文公式可得到任意给定置信度、可靠度、寿命分散条件下的寿命分散系数,可供工程上评估构件寿命时使用。

关键词：航空航天推进系统疲劳寿命可靠性对数正态分布分散系数试验评定

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Mechanism of Chronic Stress-induced Reduced Atherosclerotic Medial Area and Increased Plaque Instability in Rabbit Models of Chronic Stress

引用

Chinese Medical Journal 2018年第2期131卷 161-170页

作者： Ze-Mou Yu xiao-Tao Deng Ruo-Mei Qi lu-yan xiao Chong-Qing yang Tao Gong Deoartment of Neuroloav Beiiinq Hospital National Center of Gerontology Beijing 100730 China Denartment of Radioloav. Beiiina Hospital National Center of Gerontology Beijing 100730 China The MOH'Key Laboratory of Geriatrics Beijing Hospital National Center of Gerontology Beijing 100730 China Department of Laboratory Beijing Hospital National Center of Gerontology Beijing 100730 China Department of Pathology Beijing Hospital National Center of Gerontology Beijing 100730 China

Background： Chronic stress contributes to increased risks ofatherosclerotic diseases including heart disease, stroke, and transient ischemic attack. However, its underline mechanisms are poorly understood. This study aimed to elucidate the mechanism via which chronic stress exerts its effect on atherosclerosis （AS）. Methods： Fifty male New Zealand white rabbits were used. Aortic balloon-injury model was applied. Both social stress and physical stress methods were adopted to establish chronic stress models. The lumen stenotic degree, intimal and medial areas, maximum fibrous cap thickness, and plaque contents were measured with histological sections. Proteomic methods were applied to detect protein changes in abdominal aortas to identify the specialized mediators. Real-time reverse transcription-polymerase chain reaction was used for further verification and investigation. Results： The stress rabbits exhibited lower body weight, worse fur state, more inactivity behavior, and higher serum cortisol level. Chronic stress was significantly associated with the decreased medial area and increased plaque instability, which was manifested by thinner fibrous caps, larger lipid cores, more macrophages, and new vessels but fewer smooth muscle cells and elastic fibers. After chronic stress, the apoptosis-related genes UBE2K, BAX, b）~S, Ca.v^ase 3, Caspase 9, and P53 were upregulated, and B^Z-2/BAX was down-regulated; the angiogenesis-related genes ANG and VEGF-A were also highly expressed in atherosclerotic arteries. Conclusions： Rabbit models of chronic stress were successfully established by applying both social stress and physical stress for 8 weeks. Chronic stress can reduce AS tunica media and accelerate plaque instability by promoting apoptosis and neovascularization.

关键词： Atherosclerosis Chronic Stress: Plaque Stability Rabbit Model Tunica Media

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Zinc finger protein A20 protects rats against chronic liver allograft dysfunction

引用

World Journal of Gastroenterology 2012年第27期18卷 3537-3550页

作者： Jie yang Ming-Qing Xu lu-Nan yan xiao-Bo Chen Jiao Liu Department of Liver and Vascular Surgery Liver Transplanta-tion Center West China Hospital Sichuan University Chengdu610041 Sichuan Province China

AIM： To investigate the effect of zinc finger protein A20 on chronic liver allograft dysfunction in rats. METHODS： AIIogeneic liver transplantation from DA rats to Lewis rats was performed. Chronic liver allograft dysfunction was induced in the rats by administering low-dose tacrolimus at postoperative day （POD） 5. Hepatic overexpression of A20 was achieved by recom- binant adenovirus （rAd.）-mediated gene transfer ad- ministered intravenously every 10 d starting from POD 10. The recipient rats were injected with physiologi- cal saline, rAdEasy-A20 （1 × 109 pfu/30 g weight） or rAdEasy （1 × 109 pfu/30 g weight） every 10 d through the tail vein for 3 mo starting from POD 10. Liver tissue samples were harvested on POD 30 and POD 60. RESULTS： Liver-transplanted rats treated with only tacrolimus showed chronic allograft dysfunction with severe hepatic fibrosis. A20 overexpression ameliorated the effects on liver function, attenuated liver allograft fibrosis and prolonged the survival of the recipient rats. Treatment with A20 suppressed hepatic protein pro- duction of tumor growth factor （TGF）-β1, interleukin- 113, caspase-8, CD40, CD40L, intercellular adhesion molecule-i, vascular cell adhesion molecule-1 and E-selectin. A20 treatment suppressed liver cell apopto- sis and inhibited nuclear factor-KB activation of Kupffer cells （KCs）, liver sinusoidal endothelial cells （LSECs） and hepatic stellate cells （HSCs）, and it subsequently decreased cytokine mRNA expression in KCs and LSECs and reduced the production of TGF-β1 in HSCs. CONCluSION： A20 might prevent chronic liver allogra- ft dysfunction by re-establishing functional homeostasis of KCs, LSECs and HSCs.

关键词： Chronic allograft dysfunction Liver trans-plantation Zinc finger protein A20 Rat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

新媒体时代下少数民族题材电影传播与交流模式研究

引用

贵州民族研究 2018年第10期39卷 106-109页

作者：鲁小艳西安财经大学文学院陕西西安710061

新媒体时代,少数民族题材电影的传播与交流模式均发生了重大的改变。论文在分析新媒体时代下少数民族题材电影传播现状的基础上,以市场和政策两个导向为切入点,分析了当前少数民族题材电影传播的两种主要模式,并就如何提振少数民族题材... 详细信息

新媒体时代,少数民族题材电影的传播与交流模式均发生了重大的改变。论文在分析新媒体时代下少数民族题材电影传播现状的基础上,以市场和政策两个导向为切入点,分析了当前少数民族题材电影传播的两种主要模式,并就如何提振少数民族题材电影传播效果的模式进行初步探讨。

关键词：新媒体少数民族题材电影传播模式传播策略

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

小鼠胚胎干细胞条件培养基体外促进人角膜内皮细胞增殖的研究

引用

眼科新进展 2016年第3期36卷 231-236页

作者：鹿晓燕郑州大学第一附属医院眼科河南省郑州市450052

目的观察小鼠胚胎干细胞条件培养基(mouse embryonic stem cells conditioned medium,ESC-CM)是否可以在体外促进人角膜内皮细胞(human corneal endothelial cells,HCECs)的增殖。方法利用角膜内皮后弹力层组织块方法进行原代培养P0 HC... 详细信息

目的观察小鼠胚胎干细胞条件培养基(mouse embryonic stem cells conditioned medium,ESC-CM)是否可以在体外促进人角膜内皮细胞(human corneal endothelial cells,HCECs)的增殖。方法利用角膜内皮后弹力层组织块方法进行原代培养P0 HCECs。实验组使用含有25%ESC-CM的培养液进行培养,对照组使用普通角膜内皮细胞培养液(corneal endothelium medium,CEM)进行培养。倒置相差显微镜、反转录聚合酶链反应(reverse-transcription polymerase chain reaction,RT-PCR)鉴定HCECs;倒置相差显微镜观察细胞的形态及萌出时间;Western Blot、免疫组织化学法观察HCECs的泵相关功能蛋白(zona occludens protein-1,ZO-1)及Na^+-K^+-ATP酶的表达。Giemsa染色细胞克隆实验、免疫组织化学及流式细胞学检测Ki67阳性率的方法比较HCECs的增殖能力;流式细胞学方法检查细胞周期及细胞凋亡情况。Western Blot和免疫组织化学方法检测细胞周期负性调节蛋白P21的水平,初步探讨其可能的作用机制。结果原代培养时,25%ESC-CM组培养的HCECs P2细胞爬出,细胞形态呈典型多角形结构。CEM在P2时细胞形态变大,失去了多角形结构。25%ESC-CM组和CEM组均表达ZO-1、Na^+-K^+-ATP酶。25%ESC-CM组的Ki67阳性率、克隆形成数量、进入到细胞周期S期和G2期的比例均高于CEM组(均为P<0.05)。25%ESC-CM组的细胞凋亡数量和P21阳性率均低于CEM组(均为P<0.05)。结论 25%ESC-CM组可显著促进HCECs增殖;其作用可能是通过抑制P21蛋白的表达和抑制细胞凋亡实现的,为HCECs体外大量扩增提供了一种新方法。

关键词：胚胎干细胞角膜内皮细胞增殖

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于管网水力模型的雨水调蓄池运行效率评估

引用

中国给水排水 2012年第17期28卷 44-48页

作者：卢小艳李田董鲁燕同济大学环境科学与工程学院上海200092

初期雨水调蓄池是合流制溢流污染控制的主要措施。结合合肥市杏花排水系统调蓄池工程的初步设计,以InfoWorks CS排水管网水力模型为工具,探讨合理的调蓄池设计与运行参数,分析不同截流倍数与调蓄规模下溢流控制设施的长期运行效果。结... 详细信息

初期雨水调蓄池是合流制溢流污染控制的主要措施。结合合肥市杏花排水系统调蓄池工程的初步设计,以InfoWorks CS排水管网水力模型为工具,探讨合理的调蓄池设计与运行参数,分析不同截流倍数与调蓄规模下溢流控制设施的长期运行效果。结果表明,该排水系统调蓄池合理的最高蓄满水位和开闸水位均为8.5 m;在现状2倍截流倍数下,调蓄池宜采用4～5 mm径流深度的容积;在有限范围内增加截流倍数对溢流污染削减效果的提高作用不显著;综合考虑系统运行效果和实际应用的可操作性,推荐采用2倍截流倍数和较大的调蓄容积来达到溢流控制目标。

关键词：合流制溢流调蓄池管网水力模型调蓄容积截流倍数

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

协同创新背景下的地方高校与企业合作发展策略

引用

实验室研究与探索 2016年第9期35卷 250-253页

作者：吕小艳文衍宣广西大学新农村发展研究院广西南宁530004 广西大学化学化工学院广西南宁530004

我国校企合作虽已取得了一些实践成果,但与发达国家相比,我国的校企合作开展深度不够,特别是地方高校与企业的合作发展有待进一步提高和深化。本文从协同创新视角分析了地方高校与企业的合作需求及动因,发现加强校企深度合作是地方高校... 详细信息

我国校企合作虽已取得了一些实践成果,但与发达国家相比,我国的校企合作开展深度不够,特别是地方高校与企业的合作发展有待进一步提高和深化。本文从协同创新视角分析了地方高校与企业的合作需求及动因,发现加强校企深度合作是地方高校和企业推动协同创新必然选择,共生是地方高校与企业深度合作、协同创新的根本动力。通过加强政府引导与组织协同、共同制定校企协同创新战略、共建协同创新平台、建设有利于校企协同创新的文化氛围可以构建良好的校企共生机制,推动地方高校与企业深度合作与持续发展。

关键词：协同创新地方高校校企合作发展策略

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：