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Cinobufotalin prevents bone loss induced by ovariectomy in mice through the BMPs/SMAD and Wnt/β-catenin signaling pathways

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Animal Models and Experimental Medicine 2024年第3期7卷 208-221页

作者： Da-zhuang lu Li-jun Zeng Yang Li Ran-li Gu Meng-long Hu Ping Zhang Peng Yu Xiao Zhang Zheng-wei Xie Hao Liu Yong-sheng Zhou Department of Prosthodontics Peking University School and Hospital of StomatologyBeijingChina National Center of Stomatology BeijingChina National Clinical Research Center for Oral Diseases BeijingChina Beijing Key Laboratory of Digital Stomatology BeijingChina Department of Cariology and Endodontology Peking University School and Hospital of StomatologyBeijingChina Peking University International Cancer Institute Peking University Health Science CenterPeking UniversityBeijingChina Central Laboratory Peking University School and Hospital of StomatologyBeijingChina National Engineering Research Center of Oral Biomaterials and Digital Medical Devices BeijingChina

Background:Osteoporosis is a chronic bone disease characterized by bone loss and decreased bone ***,current anti-resorptive drugs carry a risk of various *** deep learning-based efficacy prediction system(DLEPS)is a forecasting tool that can effectively compete in drug screening and prediction based on gene expression *** study aimed to explore the protective effect and potential mechanisms of cinobufotalin(CB),a traditional Chinese medicine(TCM),on bone ***:DLEPS was employed for screening anti-osteoporotic agents according to gene profile changes in primary ***-CT,histological and morphological analysis were applied for the bone protective detection of CB,and the osteogenic differentiation/function in human bone marrow mesenchymal stem cells(hBMMSCs)were also *** underlying mechanism was verified using qRT-PCR,Western blot(WB),immunofluorescence(IF),***:A safe concentration(0.25mg/kg in vivo,0.05μM in vitro)of CB could effectively preserve bone mass in estrogen deficiency-induced bone loss and promote osteogenic differentiation/function of *** BMPs/SMAD and Wnt/β-catenin signaling pathways participated in CB-induced osteogenic differentiation,further regulating the expression of osteogenesis-associated factors,and ultimately promoting ***:Our study demonstrated that CB could significantly reverse estrogen deficiency-induced bone loss,further promoting osteogenic differentiation/function of hBMMSCs,with BMPs/SMAD and Wnt/β-catenin signaling pathways involved.

关键词： BMPs/SMAD bone loss cinobufotalin hBMMSCs osteogenesis osteoporosis Wnt/β-catenin signaling pathways

典型城市不同河流沉积物中抗生素抗性基因的时空分布特征:以石家庄为例

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环境科学 2024年第6期45卷 3176-3185页

作者：赵波宋圆梦卢梦淇白寅虎陈昊达高赛王琳静张璐璐崔建升河北科技大学环境科学与工程学院石家庄050000 河北省污染防治生物技术实验室石家庄050000 河北省水利规划设计研究院有限公司石家庄050021

河流是抗生素抗性基因(ARGs)的重要储库.然而,目前有关河流中ARGs的研究多集中在其时空分布,而较少对同一区域城市和农村河流中ARGs的种类和丰度进行比较.鉴于此,以石家庄市不同河流为例,分别在2020年12月和2021年4月,选取了2条农村河流... 详细信息

河流是抗生素抗性基因(ARGs)的重要储库.然而,目前有关河流中ARGs的研究多集中在其时空分布,而较少对同一区域城市和农村河流中ARGs的种类和丰度进行比较.鉴于此,以石家庄市不同河流为例,分别在2020年12月和2021年4月,选取了2条农村河流和3条城市河流,布设了15个采样点,采集了各个样点的沉积物,运用宏基因组测序技术分析了各样点沉积物中ARGs的种类和丰度,比较了城市和农村河流沉积物中ARGs的时空差异.结果表明:①在城市和农村河流中分别检出162种(4 776±4 452,丰度,下同)和79种ARGs(1 043±632),ARGs种类和丰度均呈城市>农村的趋势;②在城市河流中磺胺类(SAs,27%)、氨基糖苷类(AGs,26%)和多药类(MDs,15%) ARGs的相对丰度最高;而在农村河流中MDs类ARGs的相对丰度最高(65%),且城市河流中ARGs的复杂程度高于农村河流.③城市河流中SAs、AGs、MDs、四环素类(TCs)、利胆醇类(PNs)、大环内酯类(MLS)、β-内酰胺类(β-lactams)和二氨基嘧啶类(DAPs)ARGs间呈显著正相关(P <0.01),而糖肽类(GPs)ARGs与各类ARGs呈显著负相关(P <0.05和P <0.01);而在农村河流中MDs和SAs类ARGs呈显著正相关(P <0.05),氨基香豆素类(ACs)ARGs与肽类(PTs)、利福霉素类(RMs)和磷霉素类(FMs)ARGs呈显著负相关(P <0.05和P <0.01);④在时间分布上,城市河流12月和4月分别检出162种(4 776±4 452)和148种(5 673±5 626)ARGs;而农村河流12月和4月分别检出79种(1 043±632)和46种(467±183)ARGs;⑤RDA分析结果表明,城市和农村河流中的ARGs均呈时间分布差异;相关分析表明城市河流主要与工业企业数量显著相关,而农村河流则主要与牧业产值显著相关.总体而言,通过比较城市和农村河流中ARGs的时空异质性,识别其主要社会影响因子,可为后续河流中ARGs风险管控提供数据支撑.

关键词：抗生素抗性基因(ARGs) 时空分布宏基因组城市河流农村河流社会经济因子

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纳米液滴撞击高温平板壁的分子动力学模拟

物理学报 2024年第10期73卷 126-135页

作者：冯山青龚路远权生林郭亚丽沈胜强大连理工大学能源与动力学院热能综合利用技术国家地方联合工程研究中心大连116024

采用分子动力学的方法对纳米液滴撞击高温平板壁面产生的Leidenfrost现象进行了探究,分析了液滴撞击不同温度壁面对Leidenfrost现象的影响.结果表明,随着壁面温度的提升,液滴蒸发的速度更快,脱离壁面的时刻越早,脱离壁面时的速度也越大... 详细信息

采用分子动力学的方法对纳米液滴撞击高温平板壁面产生的Leidenfrost现象进行了探究,分析了液滴撞击不同温度壁面对Leidenfrost现象的影响.结果表明,随着壁面温度的提升,液滴蒸发的速度更快,脱离壁面的时刻越早,脱离壁面时的速度也越大,最终悬浮时液滴体积也越大.通过分析脱离壁面前一时刻液滴内部的密度、温度分布发现,由于高温壁面具有更高的热通量致使蒸发过程更快进而产生更厚的蒸气层,该蒸气层将阻碍换热,使得液滴在壁面温度更高时液滴内部的平均温度越低,且平均密度越小.

关键词：分子动力学液滴 Leidenfrost现象

Cr^(3+)掺杂Ca_(4)HfGe_(3)O_(12)宽带近红外荧光粉的发光特性及应用

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无机化学学报 2024年第2期40卷 345-352页

作者：绳星星肖峰吕锦彬新疆第二医学院生物医学工程学院克拉玛依834000

近红外荧光粉在生物活体成像领域展现出重要的应用前景。但活体成像用近红外荧光粉存在种类匮乏、耐温性差等瓶颈问题。采用固相法合成了宽带近红外Ca_(4)HfGe_(3)O_(12):xCr^(3+)(0≤x≤0.09)荧光粉。X射线衍射和能谱分析的结果表明Cr^... 详细信息

近红外荧光粉在生物活体成像领域展现出重要的应用前景。但活体成像用近红外荧光粉存在种类匮乏、耐温性差等瓶颈问题。采用固相法合成了宽带近红外Ca_(4)HfGe_(3)O_(12):xCr^(3+)(0≤x≤0.09)荧光粉。X射线衍射和能谱分析的结果表明Cr^(3+)离子成功进入Ca_(4)HfGe_(3)O_(12)晶格。在469 nm蓝光激发下,Ca_(4)HfGe_(3)O_(12):xCr^(3+)荧光粉发射出690~1200 nm的宽带近红外光,峰值波长为825 nm(4T2-4A2),半高宽达到141 nm,Cr^(3+)掺杂最佳浓度为0.03。依据激发光谱峰形和寿命衰减行为,证实Cr^(3+)仅占据基质中一种阳离子格位。Ca_(4)HfGe_(3)O_(12):0.03Cr^(3+)荧光粉的荧光量子效率为33.63%,该荧光粉发射光谱在400 K下的积分面积为室温下的60.5%,表明该样品具有优良的热稳定性。采用自制近红外荧光粉转换器件照射人手掌和滤波片遮挡的水果,观察到清晰地静脉血管和遮挡水果的轮廓。

关键词：热稳定性 Ca_(4)HfGe_(3)O_(12) 近红外荧光粉 Cr^(3+)离子发光材料

Proteomic profiling of cerebrospinal fluid in pediatric myelin oligodendrocyte glycoprotein antibody-associated disease

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World Journal of Pediatrics 2024年第3期20卷 259-271页

作者： Yi-Long Wang Meng-ying Zhu Zhe-Feng Yuan Xiao-Yan Ren Xiao-Tong Guo Yi Hua lu Xu Cong-ying Zhao Li-Hua Jiang Xin Zhang Guo-Xia sheng Pei-Fang Jiang Zheng-Yan Zhao Feng Gao Department of Neurology Children's HospitalZhejiang University School of MedicineHangzhou 310052China Children's Hospital Zhejiang University School of MedicineHangzhou 310052China Children's Hospital National Clinical Research Center for Child HealthZhejiang University School of MedicineHangzhou 310052China

Background Myelin oligodendrocyte glycoprotein(MOG)antibody-associated disease(MOGAD)is an autoimmune demy-elinating disorder of the central nervous *** Extracted proteins from 34 cerebrospinal fluid(CSF)samples[patients with MOGAD(MOG group,n=12);healthy controls(HC group,n=12);patients with MOG seronegative and metagenomics next-generation sequencing-negative inflammatory neurological diseases(IND group,n=10)]were processed and subjected to label-free quantitative *** partial least squares-discriminant analysis(PLS-DA)and orthogonal PLS-DA(O-PLS-DA)models were also performed based on proteomics *** analysis of differentially expressed proteins(DEPs)was performed using Gene Ontology,InterPro,and Kyoto Encyclopedia Genes and *** enzyme-linked immunosorbent assay was used to determine the complement levels in serum from patients with *** Four hundred and twenty-nine DEPs(149 upregulated and 280 downregulated proteins)were identified in the MOG group compared to the HC group according to the P value and fold change(FC).Using the O-PLS-DA model,872 differentially abundant proteins were identified with variable importance projection(VIP)scores>*** proteins(gamma-glutamyl hydrolase,cathepsin F,interalpha-trypsin inhibitor heavy chain 5,latent transforming growth factor beta-binding protein 4 and leukocyte-associated immunoglobulin-like receptor 1)overlapping between the top 30 DEPs with top-ranked P value and FC and top 30 proteins in PLS-DA VIP lists were *** analysis revealed that the dysregulated proteins in the MOG group were primarily involved in complement and coagulation cascades,cell adhesion,axon guidance,and glycosphingolipid biosynthesis compared to the HC *** The proteomic alterations in CSF samples from children with MOGAD identified in the current study might provide opportunities for developing novel biomarker candidates.

关键词： Acute disseminated encephalomyelitis Cerebrospinal fluid Complement cascades Myelin oligodendrocyte glycoprotein-associated disease Proteomics

Development of a nomogram for predicting liver transplantation prognosis in hepatocellular carcinoma

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World Journal of Gastroenterology 2024年第21期30卷 2763-2776页

作者： Li He Wan-sheng Ji Hai-Long Jin Wen-Jing lu Yuan-Yuan Zhang Hua-Guang Wang Yu-Yu Liu Shuang Qiu Meng Xu Zi-Peng Lei Qian Zheng Xiao-Li Yang Qing Zhang Department of Organ Transplantation The Third Medical Centre of Chinese PLA General HospitalBeijing 100039China School of Clinical Medicine Shandong Second Medical UniversityWeifang 261053Shandong ProvinceChina Clinical Research Center The Affiliated Hospital of Shandong Second Medical UniversityWeifang 261053Shandong ProvinceChina Department of Laboratory Medicine The Third Medical Centre of Chinese PLA General HospitalBeijing 100039China Physiatry Department Naval Aviation UniversityYantai 100071Shandong ProvinceChina

BACKGROUND At present,liver transplantation(LT)is one of the best treatments for hepatocellular carcinoma(HCC).Accurately predicting the survival status after LT can significantly improve the survival rate after LT,and ensure the best way to make rational use of liver *** To develop a model for predicting prognosis after LT in patients with *** Clinical data and follow-up information of 160 patients with HCC who underwent LT were collected and *** expression levels of alphafetoprotein(AFP),des-gamma-carboxy prothrombin,Golgi protein 73,cytokeratin-18 epitopes M30 and M65 were measured using a fully automated chemiluminescence *** best cutoff value of biomarkers was determined using the Youden *** regression analysis was used to identify the independent risk factors.A forest model was constructed using the random forest *** evaluated the accuracy of the nomogram using the area under the curve,using the calibration curve to assess consistency.A decision curve analysis(DCA)was used to evaluate the clinical utility of the *** The total tumor diameter(TTD),vascular invasion(VI),AFP,and cytokeratin-18 epitopes M30(CK18-M30)were identified as important risk factors for outcome after *** nomogram had a higher predictive accuracy than the Milan,University of California,San Francisco,and Hangzhou *** calibration curve analyses indicated a good *** survival and recurrence-free survival(RFS)of high-risk groups were significantly lower than those of low-and middle-risk groups(P<0.001).The DCA shows that the model has better clinical *** The study developed a predictive nomogram based on TTD,VI,AFP,and CK18-M30 that could accurately predict overall survival and RFS after *** can screen for patients with better postoperative prognosis,and improve longterm survival for LT patients.

关键词： Hepatocellular carcinoma Liver transplantation Liver cancer Nomogram Prognosis

Diagnostic performance of intravascular ultrasound-based fractional flow reserve in evaluating of intermediate left main stenosis

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Journal of Geriatric Cardiology 2024年第1期21卷 34-43页

作者： Yong-Gang SUI Cheng YANG Chang-Dong GUAN Yan-lu XU Na-Qiong WU Wei-Xian YANG Yong-Jian WU Ke-Fei DOU Yue-Jin YANG Shu-Bin QIAO Wei YU Bo XU sheng-Xian TU Jie QIAN Department of Cardiology Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Catheterization Laboratories Fuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina Biomedical Instrument Institute School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghaiChina National Clinical Research Center for Cardiovascular Diseases BeijingChina

BACKGROUND The recently introduced ultrasonic flow ratio(UFR),is a novel fast computational method to derive fractional flow reserve(FFR)from intravascular ultrasound(IVUS)*** the present study,we evaluate the diagnostic performance of UFR in patients with intermediate left main(LM)*** This is a prospective,single center study enrolling consecutive patients with presence of intermediated LM lesions(diameter stenosis of 30%-80%by visual estimation)underwent IVUS and FFR *** independent core laboratory assessed offline UFR and IVUS-derived minimal lumen area(MLA)in a blinded *** Both UFR and FFR were successfully achieved in 41 LM patients(mean age,62.0±9.9 years,46.3%diabetes).An acceptable correlation between UFR and FFR was identified(r=0.688,Plute numerical difference of 0.03(standard difference:0.01).The area under the curve(AUC)in diagnosis of physiologically significant coronary stenosis for UFR was 0.94(95%CI:0.87-1.01),which was significantly higher than angiographic identified stenosis>50%(AUC=0.66,Pluation of intermediate LM stenosis.

关键词： stenosis flow fractional

视觉语言模型引导的文本知识嵌入的小样本增量学习

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软件学报 2024年第5期35卷 2101-2119页

作者：姚涵涛余璐徐常胜多模态人工智能系统全国重点实验室(中国科学院自动化研究所) 北京100190 中国科学院大学人工智能学院北京100049 天津理工大学计算机科学与工程学院天津300384

真实场景往往面临数据稀缺和数据动态变化的问题,小样本增量学习的目的是利用少量数据推理数据知识并减缓模型对于旧知识的灾难性遗忘.已有的小样本增量学习的算法(CEC和FACT等)主要是利用视觉特征来调整特征编码器或者分类器,实现模型... 详细信息

真实场景往往面临数据稀缺和数据动态变化的问题,小样本增量学习的目的是利用少量数据推理数据知识并减缓模型对于旧知识的灾难性遗忘.已有的小样本增量学习的算法(CEC和FACT等)主要是利用视觉特征来调整特征编码器或者分类器,实现模型对于新数据的迁移和旧数据的抗遗忘.但是少量数据的视觉特征往往难以建模一个类别的完整特征分布,导致上述算法的泛化能力较弱.相比于视觉特征,图像类别描述的文本特征具有较好的泛化性和抗遗忘性.因此,在视觉语言模型的基础上,研究基于文本知识嵌入的小样本增量学习,通过在视觉特征中嵌入具有抗遗忘能力的文本特征,实现小样本增量学习中新旧类别数据的有效学习.具体而言,在基础学习阶段,利用视觉语言模型抽取图像的预训练视觉特征和类别的文本描述,并通过文本编码器实现预训练视觉特征到文本空间的映射.进一步利用视觉编码器融合学习到的文本特征和预训练视觉特征抽象具有高辨别能力的视觉特征.在增量学习阶段,提出类别空间引导的抗遗忘学习,利用旧数据的类别空间编码和新数据特征微调视觉编码器和文本编码器,实现新数据知识学习的同时复习旧知识.在4个数据集(CIFAR-100,CUB-200,Car-196和mini Image Net)上验证算法的有效性,证明基于视觉语言模型文本知识嵌入可以在视觉特征的基础上进一步提升小样本增量学习的鲁棒性.

关键词：小样本增量学习视觉语言模型文本知识嵌入类别空间引导的抗遗忘学习

芹糖葛根素对非酒精性脂肪肝小鼠肝脂代谢的影响

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中国临床药理学杂志 2024年第11期40卷 1598-1602页

作者：尚志胡银璐徐圣麟林莉莉上海中医药大学附属曙光医院中西医结合疫病研究所上海200021 无锡卫生高等职业技术学校药学系江苏无锡214028 天津中医药大学中药学院天津301617

目的研究芹糖葛根素对非酒精性脂肪肝(NAFLD)模型小鼠的防治作用,及其对肝脂代谢的影响。方法将32只雄性C57BL/6J小鼠随机分为正常组、模型组和低、高剂量实验组,每组8只。正常组给予普通维持饲料;其余3组饲养高脂饮食构建NAFLD模型。... 详细信息

目的研究芹糖葛根素对非酒精性脂肪肝(NAFLD)模型小鼠的防治作用,及其对肝脂代谢的影响。方法将32只雄性C57BL/6J小鼠随机分为正常组、模型组和低、高剂量实验组,每组8只。正常组给予普通维持饲料;其余3组饲养高脂饮食构建NAFLD模型。低、高剂量实验组分别灌胃给予5、10 mg·kg^(-1)芹糖葛根素;正常组与模型组均灌胃给予等量二甲基亚砜。4组小鼠每天给药1次,连续给药8周。观察小鼠体质量变化,用试剂盒检测小鼠血清中谷丙转氨酶(GPT)、谷草转氨酶(GOT)含量和肝组织中总胆固醇(TC)、三酰甘油(TG)含量,用蛋白质印迹法检测乙酰辅酶A羧化酶1(ACC1)和磷酸化5′-磷酸腺苷活化蛋白激酶(p-AMPK)蛋白的表达水平。结果正常组、模型组和低、高剂量实验组的体质量分别为(32.69±1.37)、(45.51±3.29)、(41.18±3.22)和(38.28±2.62)g,血清GPT分别为(36.53±6.44)、(134.56±39.91)、(121.54±43.38)和(75.92±25.98)U·L^(-1),小鼠血清GOT分别为(60.81±8.74)、(188.51±39.70)、(156.02±41.67)和(126.79±16.49)U·L^(-1),肝TC分别为(2.17±0.24)、(6.46±1.22)、(5.86±0.66)和(3.62±0.45)mmol·L^(-1),肝TG分别为(0.57±0.09)、(1.39±0.27)、(1.28±0.34)和(0.73±0.19)mmol·L^(-1),ACC1蛋白相对表达水平分别为1.01±0.04、2.00±0.02、1.47±0.08和1.20±0.09,p-AMPK蛋白相对表达水平分别为1.03±0.09、0.33±0.02、0.66±0.04和0.95±0.08。模型组的上述指标与高剂量实验组和正常组相比较,在统计学上差异均有统计学意义(均P<0.05)。结论芹糖葛根素通过调控AMPK信号通路,从而改善NAFLD小鼠的脂代谢。

关键词：芹糖葛根素非酒精性脂肪肝脂代谢 5′-磷酸腺苷活化蛋白激酶信号通路