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Hydrogen peroxide biosensor based on electrodeposition of zinc oxide nanoflowers onto carbon nanotubes film electrode

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Chinese Chemical Letters 2008年第3期19卷 314-318页

作者： Hui Ping Bai Xu Xiao lu Guang ming yang Yun Hui yang Department of Chemistry and Chemical Engineering Yunnan Normal University Kunming 650092 China

A new amperometric biosensor for hydrogen peroxide was developed based on adsorption of horseradish peroxidase at the glassy carbon electrode modified with zinc oxide nanoflowers produced by electrodeposition onto multi-walled carbon nanotubes （MWNTs） film. The morphology of the MWNTs/nano-ZnO electrode has been investigated by scanning electron microscopy （SEM）, and the electrochemical performance of the electrode has also been studied by amperometric method. The resulting electrode offered an excellent detection for hydrogen peroxide at -0.11 V with a linear response range of 9.9×10^-7 to 2.9×10^-3 mol/L with a correlation coefficient of 0.991, and response time 〈5 s. The biosensor displays rapid response and expanded linear response range, and excellent stability.

关键词： Multi-walled carbon nanotubes Biosensor Electrodeposition Horseradish peroxidase ZnO nanoflowers

Alemtuzumab induction therapy in highly sensitized kidney transplant recipients

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Chinese Medical Journal 2011年第5期124卷 664-668页

作者： lu Tie-ming yang Shun-liang WU Wei-zhen TAN Jian-ming Department of Urology Fuzhou General Hospital of Nanjing Command Chinese People's Liberation Army Fuzhou Fujian 350025 China

Background Immunosuppression for immunologically high-risk kidney transplant patients usually involves antithymocyte globulin induction with triple drug maintenance therapy. Alemtuzumab, a humanized anti-CD52 antibody, was expected to be a promising induction therapy agent for kidney transplantation. However, currently no consensus is available about its efficacy and safety. This study aimed to evaluate the efficacy and safety of alemtuzumab as immune induction therapy in highly sensitized kidney transplant *** In this prospective, open-label, randomized, controlled trial, we enrolled 23 highly immunological risk patients （panel reactive antibody 〉20%）. They were divided into two groups： alemtuzumab group （trial group） and anti-thymocyte globulin （ATG） group （control group）. Patients in the alemtuzumab group received intravenous alemtuzumab （15 mg） as a single dose before reperfusion. At the 24th hour post-operation, another dosage of alemtuzumab （15 mg） was given. The control group received a bolus of rabbit ATG （9 mg/kg）, which was given 2 hours before kidney transplantation and lasted until the removal of vascular clamps when the anastomoses were completed. Maintenance immunosuppression in both groups comprised standard triple therapy consisting of tacrolimus, prednisone, and mycophenolate mofetil （MMF）. Acute rejection （AR） and infection episodes were recorded, and kidney function was monitored during a 2-year follow-up. X2 test, ttest and Kaplan-Meier analysis were performed with SPSS17.0 *** Median follow-up was 338 days. In both the alemtuzumab group and ATG group, creatinine and blood urea nitrogen values in surviving recipients were similar （P 〉0.05）. White blood cell counts were significantly reduced in the alemtuzumab group for the most time points up to 6 months （P 〈0.05）. One patient receiving alemtuzumab died for acute myocardial infarction at the 65th day post-operation. Two ATG patients died for severe pulmonary infection

关键词： kidney transplantation alemtuzumab antithymocyte globulin panel reactive antibody immune induction

Effects of Plastic Film Mulching of Millet on Soil Moisture and Temperature in Semi-Arid Areas in South Ningxia of China

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Agricultural Sciences in China 2005年第11期4卷 865-871页

作者： LIAO Yun-cheng ZHANG De-qi JIA Zhi-kuan ZHANG Li lu yang-ming College of Agronomy Northwest A＆F University Yangling 712100 P.R. China

The effects of film mulching of millet on soil water content were studied in semi-arid areas in the Loess Plateau of South Ningxia, China. Different mulching methods including water micro-collecting farming （WF）, water micro-collecting farming in winter fallow （WW）, hole seeding on film （HF）, hole seeding on film in winter fallow （HW） were compared to determine the effects of mulching methods on soil water collecting and conservation during millet growth periods of 2003-2004, as well as the variation tendency of water content after rainfall, output of millet and water use efficiency （WUE）. The experimental results in the two successive years indicated that water micro-collecting farming had a better function of collecting water after rainfall, and side infiltrated water was stored under the ridges and the top layer 0-40 cm soil water changes were great. WF had obvious role in water collection and preservation of soil moisture. It effectively improved the water supply capacity by about 19.05% in the end of growth seasons. The storage of HW and WW increased by 24.9 and 7.1 mm compared with CK, and output of yield were obviously increased. Film mulching increased the yield of millet and enhanced water use efficiency （WUE）. During different growth periods, WF exhibited better water storage function with lower water consumption, and demonstrated optimal social and ecological benefits.

关键词： Millet Dynamic change of soil moisture Film mulching Water micro-collecting farming

Possible aerosol transmission of COVID-19 and special precautions in dentistry

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2020年第5期21卷 361-368页

作者： Zi-yu GE lu-ming yang Jia-jia XIA Xiao-hui FU Yan-zhen ZHANG Department of General Dentistry the Second Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310009China

Since its emergence in December 2019,corona virus disease 2019(COVID-19)has impacted several countries,affecting more than 90 thousand patients and making it a global public *** routes of transmission are direct contact,and droplet and possible aerosol *** to the unique nature of dentistry,most dental procedures generate significant amounts of droplets and aerosols,posing potential risks of infection *** the significance of aerosol transmission and its implications in dentistry can facilitate the identification and correction of negligence in daily dental *** addition to the standard precautions,some special precautions that should be implemented during an outbreak have been raised in this review.

关键词： Corona virus disease 2019(COVID-19) Aerosol Infection control

Brusatol Inhibits Proliferation,Migration,and Invasion of Nonsmall Cell lung Cancer PC-9 Cells

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World Journal of Traditional Chinese Medicine 2023年第4期9卷 454-460页

作者： lu-ming yang Wen-Min Zhou Qiao-Ru Guo Xin-Yue Fan Dong-Yu Huang Xiao-Fei Sun Jie Yuan Hua Yu Hu-Biao Chen Jian-Ye Zhang Key Laboratory of Molecular Target and Clinical Pharmacology and The State Key Laboratory of Respiratory Disease School of Pharmaceutical Sciences and The Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhouChina Department of Pharmaceutical Sciences Xinjiang Second Medical CollegeKaramay China Institute of Chinese Medical Sciences State Key Laboratory of Quality Research in Chinese MedicineUniversity of MacaoMacaoChina School of Chinese Medicine Hong Kong Baptist UniversityHong KongChina

Objective:The purpose of this study was to investigate the inhibitory effect of brusatol,a nigakilactone extracted from Brucea javanica,on lung cancer for development of therapeutic *** explored the effects of brusatol on the proliferation,migration,and invasion of lung cancer PC-9 cells in vitro and analyzed the mechanisms *** and Methods:MTT assay was used to determine the effect of brusatol on the proliferative capacity of PC-9 and H1975 cells in *** half-maximal inhibitory concentrations(IC_(50))were calculated and used as a reference for subsequent *** in the number and size of tumor cell clusters were monitored by the colony formation assay as evidence for the effect of brusatol on cell *** effect of brusatol on the migration and invasion of PC-9 cells was evaluated using wound healing and transwell assays,*** in lung cancer cells was detected using the Annexin V-FITC/propidium iodide *** correlated anticancer mechanism was detected using Western ***:The IC_(50)values of brusatol acting on PC-9 and H1975 cells were 1.58±0.30μM and 31.34±2.72μM,respectively,according to the MTT *** addition,brusatol suppressed PC-9 cell proliferation,migration,and invasion,as well as induced apoptosis,which may be related to the downregulation of epidermal growth factor receptor(EGFR),β-catenin,Akt,and STAT3 ***:Brusatol showed potent anticancer activity against lung cancer PC-9 cells,inhibiting the proliferative capacity and metastatic potential of PC-9 *** anticancer effect may be related to the downregulation of EGFR,β-catenin,Akt,and STAT3.

关键词： Antitumor brusatol lung cancer migration proliferation

Expression of ΔDNMT3B Variants and Its Association with Estrogen/Progestogen Receptor Status in Breast Cancer

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Chinese Journal of Cancer Research 2009年第3期21卷 229-234页

作者： Ning-hong Liu lu yang ming-lei Zhuo Jie Wang Shu-hang Wang Jun Zhao Hua Bai Key Laboratory of Carcinogenesis and Translational Research （Ministry of Education） Department of Thoracic Medical Oncology Peking University School of Oncology Beijing Cancer Hospital ＆ Institute Beijing 100142 China

Objective： Our previous study has showed that △DNMT3B is the predominant form of DNMT3B in non-small cell lung cancer （NSCLC）. In this study, we aimed to explore the expression patterns of the △DNMT3B variants in breast cancer and to identify whether the pattern was similar to that in NSCLC or not and its clinical significance. Methods： Expression of seven △DNMT3B variants in 59 breast cancer and the corresponding normal tissue was measured using RT-PCR. The correlations between the expressions of △DNMT3B variants and the clinical parameters including ER/PR status, clincopathologic feature and survivals were analyzed. Results： There were significant differences in the expression ratios of △DNMT3B1-7 variants between breast cancer tissues and normal tissues （P〈0.001）. The positive ratio of △DNMT3B1-7 variants were 66%, 71%, 17%, 51%, 76.2%, 50% and 61% in tumor tissue, respectively; while 16%, 8.4%, 3.38%, 3.38%, 11.8%, 13.5% and 5.08% in the corresponding normal tissue, which was different from the pattern of △DNMT3B1-7 expression in NSCLC （62%, 76%, 2.5%, 46%, 18%, 27% and 16% in tumor tissue, respectively; while 18%, 11%, 0%, 3.3%, 0%, 0% and 0% in normal lung tissue, respectively; P〈0.0001）. Expressions of △DNMT3B2, 3B4 and 3B7 were higher in the patients with negative estrogen receptor （ER） than those with positive estrogen receptor （P=0.035, P=0.0141 and P=0.0219, respectively）. △DNMT3B7 expression was higher for the patients with negative progestogen receptor （PR） compared to those with positive progestogen receptor （P=0.0379）. Expression ratio of △DNMT3B5 in stage Ⅲ tumors is lower than that in stage Ⅰ/Ⅱ ones （P= 0.041）. But we did not find any relation between the △DNMT3B variants and the patients＇ survival. Conclusion： The pattern of △DNMT3B variants in breast cancer is different from that in NSCLC. Expressions of △DNMT3B2, 3B4 and 3B7 are associated with estrogen receptors status. While △DNMT3B7 is associated with progestogen receptor. No relation

关键词： △DNMT3B variants RT-PCR Breast cancer Estrogen receptor Progestogen receptor

Dynamics of Primary Events in the Photocycle of Excited Bacteriorhodopsin

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Acta Biochimica et Biophysica Sinica 2004年第11期36卷 724-728页

作者： Jun-Jun lu, ming ming, Yi yang, Jia WU, Bo LI, Jian-Dong DING, Qing-Guo LI, and Shi-Xiong QIANDepartment of Physics, Fudan University, Shanghai 200433, China Department of Macromolecular Science, Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433, China Department of Physiology and Biophysics, School of Life Sciences, Fudan University, Shanghai 200433, China Department of Physics Fudan University Shanghai 200433 China

Transient dynamic behavior of the excited bacteriorhodopsin (BR), which was isolated from the strain H. salinarum, was studied at excitation wavelength from 585 to 639 nm. With the one-color femtosecond (fs) pump-probe technique, we revealed the primary events in BR's photocycle that took place in an ultrafast time scale. From the analysis of the decay components of the dynamical traces, it was evident that the isomerization of the retinal

关键词： bacteriorhodopsin transient dynamics primary events femtosecond laser pump-probe technique

基于PLC的船舶发电机组负荷功率自动控制系统设计

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舰船科学技术 2019年第2期41卷 91-93页

作者：卢明阳仰恩大学工程技术学院福建泉州362014

现代船舶对于发电机组的要求越来越高,而且工况也越来越复杂,经常会出现负载功率剧烈变化的情况,对船舶发电机组负荷功率进行自动控制、监测和管理对于保障船舶电站的稳定安全工作具有非常重要的意义。本文提出一种基于三菱PLC的发电机... 详细信息

现代船舶对于发电机组的要求越来越高,而且工况也越来越复杂,经常会出现负载功率剧烈变化的情况,对船舶发电机组负荷功率进行自动控制、监测和管理对于保障船舶电站的稳定安全工作具有非常重要的意义。本文提出一种基于三菱PLC的发电机组负荷功率自动控制系统,详细分析了系统的需求,对发电机组的并车原理进行总结,最后对系统的整体结构和程序进行设计。系统能够通过发电机组的并车和解列以及负载调节等实现功率的自动调节,系统具有很高的稳定性和抗干扰性。

关键词：功率调节并车发电机组 PLC

新中国成立以来基层党组织功能调适的动因、历程与启示

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内蒙古社会科学 2024年第4期45卷 72-81+213页

作者：张浩鲁杜阳明中山大学马克思主义学院

功能调适是政党等政治主体为适应时代变化和实践需要对自身功能进行有针对性的调整。新中国成立以来，基于我国社会主要矛盾的发展转化、不同时期或阶段党的中心任务的现实需求、基层党组织先进性建设的主体自觉以及我国发展历史方位的... 详细信息

功能调适是政党等政治主体为适应时代变化和实践需要对自身功能进行有针对性的调整。新中国成立以来，基于我国社会主要矛盾的发展转化、不同时期或阶段党的中心任务的现实需求、基层党组织先进性建设的主体自觉以及我国发展历史方位的深刻演进等多方面动因，党的基层组织对自身功能进行了深刻调整。新中国成立以来基层党组织功能调适的历程分为三个阶段：社会主义革命和建设时期，基层党组织以实现由新民主主义向社会主义的转变为目的进行功能调适；改革开放和社会主义现代化建设新时期，基层党组织以探索社会主义建设的正确道路和追求社会生产力的稳步增长为目的进行功能调适；中国特色社会主义进入新时代，基层党组织以奋进“两个一百年”奋斗目标为目的进行功能调适。新中国成立以来基层党组织的功能调适历程告诉我们，基层党组织的功能调适要坚持以夯实党的执政根基为根本目的，以厘清基层党组织的角色定位为前提基础，以执行党的政治路线为原则遵循，以调动各方积极性为实践要义，以解决现实问题为方向引领。

关键词：基层党组织功能调适生成动因历史进程现实启示