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Plutella xylostella granulovirus late gene promoter activity in the context of the Autographa californica multiple nucleopolyhedrovirus genome

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Virologica Sinica 2016年第3期31卷 229-239页

作者： He-lin Ren Yuan Hu Ya-Jun Guo lu-lin li Hubei Key Laboratory of Genetic Regulation and Integrative Biology College of Life SciencesCentral China Normal University

Within Baculoviridae, little is known about the molecular mechanisms of replication in betabaculoviruses, despite extensive studies in alphabaculoviruses. In this study, the promoters of nine late genes of the betabaculovirus Plutella xylostella granulovirus(Plxy GV) were cloned into a transient expression vector and the alphabaculovirus Autographa californica multiple nucleopolyhedrovirus(Ac MNPV) genome, and compared with homologous late gene promoters of Ac MNPV in Sf9 cells. In transient expression assays, all Plxy GV late promoters were activated in cells transfected with the individual reporter plasmids together with an Ac MNPV bacmid. In infected cells, reporter gene expression levels with the promoters of Plxy GV e18 and Ac MNPV vp39 and gp41 were significantly higher than those of the corresponding Ac MNPV or Plxy GV promoters,which had fewer late promoter motifs. Observed expression levels were lower for the Plxy GV p6.9,pk1, gran, p10 a, and p10 b promoters than for the corresponding Ac MNPV promoters, despite equal numbers of late promoter motifs, indicating that species-specific elements contained in some late promoters were favored by the native viral RNA polymerases for optimal transcription. The 8-nt sequence TAAATAAG encompassing the ATAAG motif was conserved in the Ac MNPV polh, p10,and pk1 promoters. The 5-nt sequence CAATT located 4 or 5 nt upstream of the T/ATAAG motif was conserved in the promoters of Plxy GV gran, p10 c, and pk1. The results of this study demonstrated that Plxy GV late gene promoters could be effectively activated by the RNA polymerase from Ac MNPV, implying that late gene expression systems are regulated by similar mechanisms in alphabaculoviruses and betabaculoviruses.

关键词： Plutella xylostella granulovirus(PlxyGV) Autographa californica multiple nucleopolyhedrovirus(AcMNPV) late gene expression

Application of systems biology to the study of chronic kidney disease

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Chinese Medical Journal 2012年第14期125卷 2603-2609页

作者： CAO Yu-han lu lin-li ZHANG Jian-dong liU Bi-cheng Institute of Nephrology Zhong Da Hospital Southeast UniversityNanjing Jiangsu 210009 China

Chronic kidney disease （CKD） is a major public health problem that affects about 10% of the general population. Current approaches to characterize the category and progression of CKD are normally based on renal histopathological results and clinical parameters. However, this information is not sufficient to predict CKD progression risk reliably or to guide preventive interventions. Nowadays, the appearance of systems biology has brought forward the concepts of ＂-omics＂ technologies, including genomics, transcriptomics, proteomics, and metabolomics. Systems biology, together with molecular analysis approaches such as microarray analysis, genome-wide association studies （GWAS）, and serial analysis of gene expression （SAGE）, has provided the framework for a comprehensive analysis of renal disease and serves as a starting point for generating novel molecular diagnostic tools for use in nephrology. In particular, analysis of urinary mRNA and protein levels is rapidly evolving as a non-invasive approach for CKD monitoring. All these systems biological molecular approaches are required for application of the concept of ＂personalized medicine＂ to progressive CKD, which will result in tailoring therapy for each patient, in contrast to the ＂one-size-fits-all＂ therapies currently in use.

关键词： ' systems biology chronic kidney disease, microarray analysis, genome-wide association study,serial analysis of gene expression

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存储测试装置聚氨酯灌封料减振特性研究

北京理工大学学报 2016年第10期36卷 1019-1024页

作者：鲁林李晓峰陈玟北京理工大学机电学院北京100081 北京理工大学机械与车辆学院北京100081

为研究聚氨酯封料的减振特性,通过霍普金森杆实验获得了不同密度聚氨酯材料的力学参数,对运用该参数进行的侵彻硬目标仿真获得的结果与空气炮实验测得数据进行了相关性计算,二者间具有较高的相关系数表明仿真模型及材料参数有效,在此基... 详细信息

为研究聚氨酯封料的减振特性,通过霍普金森杆实验获得了不同密度聚氨酯材料的力学参数,对运用该参数进行的侵彻硬目标仿真获得的结果与空气炮实验测得数据进行了相关性计算,二者间具有较高的相关系数表明仿真模型及材料参数有效,在此基础上,基于仿真数据和动力学模型进行了灌封后的测试体传递函数的辨识,分析了该传递函数的幅频特性,对不同密度聚氨酯灌封下的存储测试装置结构减振特性给出了定量评估.

关键词：聚氨酯本构参数霍普金森杆传递函数存储测试

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不同叶顶间隙对泵喷推进器性能的影响

华中科技大学学报（自然科学版） 2017年第8期45卷 110-114页

作者：鹿麟李强高跃飞中北大学机电工程学院山西太原030051

基于均质多相流的RANS方程与分块网格技术,结合剪切应力SSTk-ω湍流模型,对E779A桨进行了非空化与空化数值模拟,数值计算结果与实验值符合较好,验证了数值计算方法的合理性与有效性.在此基础上,对不同叶顶间隙的泵喷推进器进行了非空化... 详细信息

基于均质多相流的RANS方程与分块网格技术,结合剪切应力SSTk-ω湍流模型,对E779A桨进行了非空化与空化数值模拟,数值计算结果与实验值符合较好,验证了数值计算方法的合理性与有效性.在此基础上,对不同叶顶间隙的泵喷推进器进行了非空化与空化数值模拟计算,重点对叶顶间隙处流场进行了精细后处理.结果表明:未空化时,间隙尺寸越大,泵喷推进器整体效率值越低,最大间隙与最小间隙之间的效率降幅最大为8.62%,叶顶间隙尺寸直接对泵喷推进器的最优进速系数产生影响.当空化发生时,在最优进速系数下的推进器效率下降幅度最小;间隙尺寸越大,空化后的推进器整体效率越低;在较高转速下,较大间隙的空化面积在叶稍处有更为明显的激增量.

关键词：泵喷推进器叶顶间隙动力推进系统数值模拟顶隙泄涡

Preliminary Clinical Observation of Arteriovenous Sheathotomy for Treatment of Branch Retinal Vein Occlusion

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Eye Science 2003年第1期19卷 33-38页

作者： lin lu, Yonghao li, Changxian Yi, Mei li, Xiulan lu, Jinglin ZhangZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China ZhongshanOphthalmicCenter SunFat-senUniversityGuangzhou510060China

Purpose: To document the anatomic and functional improvement of six patients withbranch retinal vein occlusion (BRVO) following successful arteriovenous adventitialsheathotomy (AAS).Methods: Retrospective study of 6 patients (6 eyes) with BRVO treated with AAS. Allpatients were not eligible for laser photocoagulation and had both macular edema andintraretinal hemorrhage. The visual acuity was in the range of 0.4 to 0.02. All patientsunderwent pars plana vitrectomy and AAS. The clinical improvement was determined byfundus photograph, fluorescein angiography (FAG), optical coherence tomography(OCT) and multifocal electroretinography (ERG). All patients were followedpostoperatively for an average of 20 months ranging from 12 to 24 ***: Sheathotomy and decompression of the arteriole/venule (A/V) crossing wereachieved in all 6 patients. 5 patients have improved their best-corrected visual acuity 4lines or more. The best one could reach to 1.0. One month after the operation, fundusphotograph and FAG demonstrated the resolution of intraretinal hemorrhage, reduction ofnon-perfusion area and apparent resolution of retinal venous dilation and tortuosity. OCTconfirmed remarkable reduction of retinal thickness. The microcysts at the foveadiminished. Multifocal ERG showed the recovery of the central peak at the macular andthe peripheral response density. However, capillary nonperfusion area andmicroaneurysm were found out by FAG in four patients at the points distal to thesheathotomy three months after the ***: Anatomic and functional improvement of retina can be achieved in patientswith BRV0 through AAS. However, the capillary nonperfusion and microaneurysm mayfollow this surgical procedure in some cases that need further treatment with laserphotocoagulation. The better visual improvement may be expected in the patients withearlier surgical intervention.

关键词：视网膜分支血管阻塞临床特点荧光血管造影术 X线检查治疗

Advances in the Development of Gradient Scaffolds Made of Nano-Micromaterials for Musculoskeletal Tissue Regeneration

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NANO-MICRO LETTERS 2025年第1期17卷 1-46页

作者： Fang, Lei lin, Xiaoqi Xu, Ruian liu, lu Zhang, Yu Tian, Feng li, Jiao Jiao Xue, Jiajia Beijing Univ Chem Technol Beijing Lab Biomed Mat Beijing 100029 Peoples R China Beijing Univ Chem Technol State Key Lab Organ Inorgan Composites Beijing 100029 Peoples R China Univ Technol Sydney Fac Engn & IT Sch Biomed Engn Sydney NSW 2007 Australia

This review highlights the gradient variations in the structural composition of musculoskeletal tissues and comprehensively examines recent progress in the fabrication and application of biomimetic gradient scaffolds for musculoskeletal *** challenges and prospects of gradient scaffolds for clinical application are discussed. The intricate hierarchical structure of musculoskeletal tissues, including bone and interface tissues, necessitates the use of complex scaffold designs and material structures to serve as tissue-engineered substitutes. This has led to growing interest in the development of gradient bone scaffolds with hierarchical structures mimicking the extracellular matrix of native tissues to achieve improved therapeutic outcomes. Building on the anatomical characteristics of bone and interfacial tissues, this review provides a summary of current strategies used to design and fabricate biomimetic gradient scaffolds for repairing musculoskeletal tissues, specifically focusing on methods used to construct compositional and structural gradients within the scaffolds. The latest applications of gradient scaffolds for the regeneration of bone, osteochondral, and tendon-to-bone interfaces are presented. Furthermore, the current progress of testing gradient scaffolds in physiologically relevant animal models of skeletal repair is discussed, as well as the challenges and prospects of moving these scaffolds into clinical application for treating musculoskeletal injuries.

关键词： Gradient scaffolds Musculoskeletal tissues Advanced manufacturing Biomaterials Tissue regeneration

基于二水草酸镁(MgC_2O_4·2H_2O)的无水碳酸镁(MgCO_3)的高压制备和表征

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物理学报 2017年第3期66卷 123-129页

作者：梁文李泽明王璐颖陈琳李和平中国科学院地球化学研究所贵阳550081 中国科学院大学北京100049

无水碳酸镁因为其重要的研究价值和广泛的应用而备受关注,因此探索无水碳酸镁的制备方法已经成为材料加工的一个重要课题.以二水草酸镁为起始原料,使用高温高压反应法成功地合成了高纯度的无水碳酸镁,对所得样品分别进行了粉末X射线衍射... 详细信息

无水碳酸镁因为其重要的研究价值和广泛的应用而备受关注,因此探索无水碳酸镁的制备方法已经成为材料加工的一个重要课题.以二水草酸镁为起始原料,使用高温高压反应法成功地合成了高纯度的无水碳酸镁,对所得样品分别进行了粉末X射线衍射和Raman光谱的表征.通过热重分析以及尝试不同合成条件,给出了二水草酸镁-碳酸镁P-T相图并解释了高温高压合成碳酸镁的原理.利用偏光显微镜观察样品碳酸镁形貌、晶粒度以及晶体解理面,同时对样品的单晶粒做微区电子探针分析,给出了样品的镁含量.

关键词：无水碳酸镁高压合成

Fine-tuning the spatial allocation of phytohormones for smart canopy, smart breeding, and smart agriculture

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Science China life Sciences 2025年第1期 309-310页

作者： Tao Zhou lin li National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University HuBei HongShan Laboratory

The dual challenges of global climate change and population growth have increased demand for higher crop yields per unit area. The innovative technology of smart agriculture aims to synergize breeding, cultivation, and production through the application of biological and informatics technologies. Historically, the “Green Revolution” introduced semidwarf varieties of certain key crops by utilizing mutations affecting gibberellin pathways, which significantly increased planting densities and yields.

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干涉型光子晶体光纤陀螺技术研究

光学学报 2018年第3期38卷 51-59页

作者：杨远洪杨福铃陆林李慧李帅北京航空航天大学仪器科学与光电工程学院北京100191

为满足空间和其他领域的高精度制导导航控制系统的迫切需求,开展了基于实芯保偏光子晶体光纤(PMPCFs)的干涉型光子晶体光纤陀螺(PCFOG)的技术研究,研究和开发了PCFOG专用PM-PCFs和掺铒PCFs(EDPCFs)的制备技术、PCFs耦合技术和熔接工艺... 详细信息

为满足空间和其他领域的高精度制导导航控制系统的迫切需求,开展了基于实芯保偏光子晶体光纤(PMPCFs)的干涉型光子晶体光纤陀螺(PCFOG)的技术研究,研究和开发了PCFOG专用PM-PCFs和掺铒PCFs(EDPCFs)的制备技术、PCFs耦合技术和熔接工艺等关键技术,研制出了PCFOG原型样机并进行了典型环境实验。研究结果表明,PCFOG在精度和环境适应性等方面都具备明显优势,具备工程应用条件。

关键词：遥感光子晶体光纤光纤陀螺温度辐射