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S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer

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Cancer Communications 2021年第2期41卷 154-170页

作者： Si Li jun Zhang Senmi Qian Xuesong Wu liang Sun Tianyi Ling Yao Jin Wenxiao Li Lichao Sun Maode Lai Fangying Xu Department of Pathology and Pathophysiology and Department of General Surgery of the Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang 310058P.R.China Department of Pathology and Pathophysiology Zhejiang University School of MedicineHangzhouZhejiang 310058P.R.China Key Laboratory of Disease Proteomics of Zhejiang Province Zhejiang University School of MedicineHangzhouZhejiang 310058P.R.China State Key Laboratory of Molecular Oncology National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100021P.R.China

Background:The transforming growth factor-β(TGF-β)pathway plays a pivotal role in inducing epithelial-mesenchymal transition(EMT),which is a key step in cancer invasion and ***,the regulatory mechanism of TGF-βin inducing EMT in colorectal cancer(CRC)has not been fully *** previous studies,it was found that S100A8 may regulate *** study aimed to clarify the role of S100A8 in TGF-β-induced EMT and explore the underlying mechanism in ***:S100A8 and upstream transcription factor 2(USF2)expression was detected by immunohistochemistry in 412 CRC ***-Meier survival analysis was *** vitro,Western blot,and migration and invasion assays were performed to investigate the effects of S100A8 and USF2 on TGF-β-induced *** metastasis models were used to determine in vivo metastasis *** reporter and chromatin immunoprecipitation assay were used to explore the role of USF2 on S100A8 ***:During TGF-β-induced EMT in CRC cells,S100A8 and the transcription factor USF2 were upregulated.S100A8 promoted cell migration and invasion and ***2 transcriptionally regulated S100A8 expression by directly binding to its promoter ***,TGF-βenhanced the USF2/S100A8 signaling axis of CRC cells whereas extracellular S100A8 inhibited the USF2/S100A8 axis of CRC cells.S100A8 expression in tumor cells was associated with poor overall survival in ***2 expression was positively related to S100A8 expression in tumor cells but negatively related to S100A8-positive stromal ***:TGF-βwas found to promote EMT and metastasis through the USF2/S100A8 axis in CRC while extracellular S100A8 suppressed the USF2/S100A8 ***2 was identified as an important switch on the intracellular and extracellular S100A8 feedback loop.

关键词： colorectal cancer epithelial-mesenchymal transition metastasis prognosis transforming growth factor-β upstream transcription factor 2 S100 calcium-binding protein A8

Comparison of Three Influenza Surveillance Data Sources for Timely Detection of Epidemic Onset — Chengdu City, Sichuan Province and Beijing Municipality, China, 2017–2023

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China CDC weekly 2024年第36期6卷 918-923,I0004-I0006页

作者： Mingyue Pan Ying Shen Yao Wang lu Long Xunbo Du Ying Sun Daitao Zhang Hui Yao Yonghong Liu Peng Yang Quanyi Wang Xiaoli Wang liang Wang Beijing Center for Disease Prevention and Control BeijingChina Beijing Research Center for Respiratory Infectious Diseases BeijingChina Chengdu Center for Disease Control and Prevention Chengdu CitySichuan ProvinceChina

What is already known about this topic?The syndromic surveillance system,exemplified by the influenza-like illness(ILI)surveillance system,has long been crucial in providing early warnings of influenza *** is added by this report?The analysis revealed that employing reported influenza case data from the nationwide Notifiable Infectious Diseases Reporting Information System(NIDRIS)enhanced the early detection of influenza epidemics,particularly within the context of multiple respiratory pathogens circulating *** are the implications for public health practice?The NIDRIS,characterized by its extensive coverage,obligatory reporting,high specificity,and real-time data transmission,offers a valuable tool for the effective early detection of influenza *** this system could enhance preparedness and responses to such health crises,potentially mitigating their impact on public health.

关键词： nationwide system specificity

Preclinical evaluation of herpes simplex virus armed with granulocyte-macrophage colony-stimulating factor in pancreatic carcinoma

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World Journal of Gastroenterology 2013年第31期19卷 5138-5143页

作者： Hao Liu Shou-jun Yuan Ying-Tai Chen Yi-Bin Xie liang Cui Wei-Zhi Yang De-Xuan Yang Yan-Tao Tian Department of Abdominal Surgery Cancer Hospital Chinese Academy of Medical Sciences Peking Union Medical College Pharmacological and Toxi-cological Laboratory Institute of Radiation Medicine Medical Radiobiology Laboratory Cancer Hospital Chinese Academy of Medical Science Peking Union Medical College

AIM: To investigate the therapeutic efficacy and mechanisms of action of oncolytic-herpes-simplex-virus encoding granulocyte-macrophage colony-stimulating factor(HSVGM-CSF) in pancreatic ***: Tumor blocks were homogenized in a sterile grinder in *** homogenate was injected into the right armpit of each *** vaccination,the mice were randomly assigned into four groups: a control group,a high dose HSVGM-CSFgroup [1 × 107plaque forming units(pfu)/tumor],a medium dose HSVGM-CSF group(5 × 106pfu/tumor) and a low dose HSVGM-CSF group(5 × 105pfu/tumor).After initiation of drug administration,body weights and tumor diameters were measured every 3 *** days later,after decapitation of the animal by cervical dislocation,each tumor was isolated,weighed and stored in 10% formaldehyde *** drug effectiveness was evaluated according to the weight,volume and relative volume change of each ***,GM-CSF protein levels in serum were assayed by enzyme-linked immunosorbent assays at 1,2,3 and 4 d after injection of ***: Injection of the recombinant mouse HSV encoding GM-CSF resulted in a significant reduction in tumor growth compared to the control group,and dosedependent effects were observed: the relative tumor proliferation rates of the low dose,medium dose and high dose groups on 15 d after injection were 45.5%,55.2% and 65.5%,*** inhibition rates of the tumor weights of the low,middle,and high dose groups were 41.4%,46.7% and 50.5%,***,the production of GM-CSF was significantly increased in the mice infected with *** increase in the GM-CSF level was more pronounced in the high dose group compared to the other two dose ***: Our study provides the first evidence that HSVGM-CSFcould inhibit the growth of pancreatic *** enhanced GM-CSF expression might be responsible for the phenomenon.

关键词： Pancreatic carcinoma Gene therapy Animal test Herpes-simplex-virus encoding granulocytemacrophage colony-stimulating factor

Regulation of hepatic lipid metabolism by intestine epithelium-derived exosomes

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Life Metabolism 2023年第6期2卷 42-57页

作者： Tiange Feng Yuan liang Lijun Sun lu Feng Jiajie Min Michael W.Mulholland Yue Yin Weizhen Zhang Department of Physiology and Pathophysiology School of Basic Medical Sciencesand Key Laboratory of Molecular Cardiovascular ScienceMinistry of EducationPeking UniversityBeijing 100191China Department of Surgery University of Michigan Medical CenterAnn ArborMI 48109United States Department of Pharmacology School of Basic Medical Sciencesand Key Laboratory of Molecular Cardiovascular ScienceMinistry of EducationPeking UniversityBeijing 100191China

The“gut-liver axis”is critical for the control of hepatic lipid homeostasis,where the intestine affects the liver through multiple pathways,such as nutrient uptake,gastrointestinal hormone release,and gut microbiota *** intestine-originated exosomes mediate the gut’s influence on liver steatosis remains ***,we aimed to determine whether intestinal epithelium-derived exosomes(intExos)contribute to the regulation of hepatic lipid *** found that mouse intExos could be taken up by hepatic *** fed high-fat diet(HFD)received intExos showed strong resistance to liver *** sequencing of intExos indicated the correlation between miR-21a-5p/miR-145a-5p and hepatic lipid *** liver overexpression of miR-21a-5p and intExos containing miR-21a-5p alleviated hepatic steatosis in mice fed with ***,miR-21a-5p suppressed the expression of Ccl1(C-C motif chemokine ligand 1)in macrophages,as well as lipid transport genes Cd36(cluster of differentiation 36)and Fabp7(fatty acid binding protein 7)in ***-specific inhibition of miR-145a-5p significantly reduced hepatic lipid accumulation in mice fed with HFD through negatively regulating the expression of Btg1(BTG anti-proliferation factor 1),leading to an increase of stearoyl-CoA desaturase-1 and *** study demonstrates that intExos regulate hepatic lipid metabolism and non-alcoholic fatty liver dis-ease(NAFLD)progression via miR-21a-5p and miR-145a-5p pathways,providing novel mediators for the gut-liver crosstalk and potential targets for regulating hepatic lipid metabolism.

关键词： exosome non-alcoholic fatty liver disease miR-21a-5p miR-145a-5p lipid metabolism

PpERF3 positively regulates ABA biosynthesis by activating PpNCED2/3 transcription during fruit ripening in peach

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Horticulture Research 2019年第1期6卷 1535-1544页

作者： Xiaobei Wang Wenfang Zeng Yifeng Ding Yan Wang liang Niu Jia-Long Yao Lei Pan Zhenhua lu Guochao Cui Guohuai Li Zhiqiang Wang Zhengzhou Fruit Research Institute Chinese Academy of Agricultural Sciences450009 ZhengzhouChina Key Laboratory of Horticultural Plant Biology Ministry of EducationHuazhong Agricultural University430070 WuhanChina The New Zealand Institute for Plant&Food Research Limited AucklandNew Zealand

The plant hormone ethylene regulates ripening in climacteric *** phytohormone abscisic acid(ABA)affects ethylene biosynthesis,but whether ethylene influences ABA biosynthesis is *** explore this possibility,we investigated the interactions between the ABA biosynthesis genes PpNCED2/3 and the ethylene response transcription factor PpERF3 in peach *** ABA content increased during fruit maturation and reached a peak at stage S4 *** increase was greatly inhibited by the ethylene inhibitor 1-MCP,which also suppressed PpERF3 ***3 shared a similar expression profile with PpNCED2/3,encoding a rate-limiting enzyme involved in ABA biosynthesis,during fruit ripening.A yeast one-hybrid assay suggested that the nuclear-localized PpERF3 might bind to the promoters of PpNCED2/***3 increased the expression of PpNCED2/3 as shown by dual-luciferase reporters,promoter-GUS assays and transient expression analyses in peach ***,these results suggest that ethylene promotes ABA biosynthesis through PpERF3’s regulation of the expression of ABA biosynthesis genes PpNCED2/3.

关键词： ERF limiting fruit

Determination of Focal Depths of the MS5.8 Alxa Left Banner,Inner Mongolia Earthquake Sequence

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Earthquake Research in China 2018年第2期32卷 245-253页

作者： Li Bin Wang Shubo Wei Jianmin Zhang Haoming Liu Yongmei Wu jun Hao liang Earthquake Agency of Inner Mongolia Autonomous Region Hohhot 010010China

Using the double-difference earthquake location algorithm,the deterministic method (PTD method) and the CAP seismic moment tensor inversion method,the paper selects the primary waveform data of 78 earthquakes recorded by the "China Earthquake Science Array Probe Project in the Northern Part of North South Seismic Belt ",the "China Earthquake Scientific Exploration Array Data Center"of Institute of Geophysics,China Earthquake Administration,and the Inner Mongolia Digital Seismic Network to calculate the focal depths of the mainshock and the seismic sequence of the M_S5.8 Alxa Left Banner earthquake in Inner Mongolia. The results show that the focal depth of the main shock is 20. 6 km,determined by the double-difference earthquake location method,18. 1 km by the PTD method,and 19. 2 km by the CAP method. The focal depth of the earthquake sequence calculated by the double-difference location method is larger. The deterministic method (PTD method) and double-difference location method are the methods that fit the tectonic characteristics of the seismic source area,and the CAP method is suitable for larger earthquakes.

关键词： Earthquake sequence Focal depth Double-difference earthquake location method PTD method CAP method

Combined detection of plasma GATA5 and SFRP2 methylation is a valid noninvasive biomarker for colorectal cancer and adenomas

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World Journal of Gastroenterology 2015年第9期21卷 2629-2637页

作者： Xie Zhang Yu-Fei Song Hong-Na lu Dan-Ping Wang Xue-Song Zhang Shi-liang Huang Bei-Lei Sun Zhi-Gang Huang Department of Gastroenterology Ningbo Medical Treatment Center Li Huili Hospital School of Medicine Ningbo University Department of Gastroenterology Zhejiang Provincial People’s Hospital

AIM:To investigate GATA5,SFRP2,and ITGA4 methylation in plasma DNA as noninvasive biomarkers for colorectal cancer(CRC) or ***:There were 57 CRC patients,30 adenomas patients,and 47 control patients enrolled in this ***-specific polymerase chain reaction was used to determine the promoter methylation status of GATA5,SFRP2,and ITGA4 genes in plasma DNA,and their association with clinical outcome in *** predictive ability of GATA5,SFRP2,and ITGA4 methylation,individually or in combination,to detect CRC or adenomas was further ***:Hypermethylated GATA5 was detected in plasma in 61.4%(35/57) of CRC cases,43.33%(13/30) of adenoma cases,and 21.28%(10/47) of control *** hypermethylation of SFRP2 was detected in 54.39%(31/57),40.00%(12/30),and 27.66%(13/47) in plasma samples from CRC,adenomas,and controls,***4 methylation was detected in 36.84%(21/57) of plasma samples of CRC patients and in 30.00%(9/30) of plasma samples from patients with colorectal adenomas,and the specificity of this individual biomarker was 80.85%(9/47).Moreover,GATA5 methylation in the plasma was significantly correlated with larger tumor size(P =0.019),differentiation status(P =0.038),TNM stage(P =0.008),and lymph node metastasis(P =0.008).SFRP2 and ITGA4 methylation in plasma significantly correlated with differentiation status(SFRP2,P =0.012; ITGA4,P =0.007),TNM stage(SFRP2,P =0.034; ITGA4,P =0.021),and lymph node metastasis(SFRP2,P =0.034; ITGA4,P =0.021).From the perspective of predictive power and cost-performance,using GATA5 and SFRP2 together as methylation markers seemed the most favorable predictor for CRC(OR =8.06;95%CI:2.54-25.5; P luSION:A combination of GATA5 and SFRP2 methylation could be promising as a marker for the detection and diagnosis of CRC and adenomas.

关键词： Colorectal cancer GATA binding protein 5 Secreted

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劳动生产率增速变动与日本经济长期低迷

日本学刊 2014年第6期 93-109页

作者：梁军曲阜师范大学经济学院

与20世纪80年代相比,90年代以来日本的劳动生产率增速呈现出显著下降趋势。企业员工培训的相对缺失、就业人口年龄结构的老龄化、技术进步步伐的减缓、产业结构调整的滞后、国民投资率的下降、国内外自然条件的恶化等一系列因素,导致了... 详细信息

与20世纪80年代相比,90年代以来日本的劳动生产率增速呈现出显著下降趋势。企业员工培训的相对缺失、就业人口年龄结构的老龄化、技术进步步伐的减缓、产业结构调整的滞后、国民投资率的下降、国内外自然条件的恶化等一系列因素,导致了日本劳动生产率增速的下降。劳动生产率增速下降,抑制了日本潜在产出的增长,与需求不足引发的产出缺口,共同导致了日本经济增长的长期低迷。

关键词：日本经济劳动生产率潜在产出产出缺口

抗阻运动对慢性癫痫大鼠癫痫发作和记忆缺陷的影响

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基因组学与应用生物学 2019年第10期38卷 4857-4863页

作者：梁军三亚航空旅游职业学院

为了揭示抗阻运动对慢性癫痫大鼠癫痫发作和记忆缺陷的影响,本研究将雄性Wistar大鼠随机分为4组,即抗阻运动癫痫组(REG)、抗阻运动对照组(RCG)、癫痫组(EG)和对照组(CG),每组20只。通过腹腔注射毛果芸香碱诱导癫痫,然后REG组和RCG组大... 详细信息

为了揭示抗阻运动对慢性癫痫大鼠癫痫发作和记忆缺陷的影响,本研究将雄性Wistar大鼠随机分为4组,即抗阻运动癫痫组(REG)、抗阻运动对照组(RCG)、癫痫组(EG)和对照组(CG),每组20只。通过腹腔注射毛果芸香碱诱导癫痫,然后REG组和RCG组大鼠进行4周的抗阻运动,记录各组大鼠的癫痫发作次数,并记录抑制性回避试验中大鼠进入黑色盒子的潜伏期,检测各组大鼠海马的IGF-1、BDNF、CREB、ERK和mTOR水平。研究显示,抗阻运动后,EG组的癫痫发作次数显著高于REG组。REG组的潜伏期显著高于EG组。抗阻运动后,EG组大鼠海马IGF-1蛋白水平显著高于REG组。此外,EG组大鼠海马BDNF蛋白水平显著低于REG组。REG组的ERK和mTOR磷酸化水平显著高于EG组。4组大鼠海马总CREB表达水平及CREB磷酸化水平均无显著差异。本研究表明,抗阻运动可有效降低癫痫大鼠的癫痫发作次数,并提高大鼠记忆力,减轻癫痫引起的记忆缺陷。抗阻运动抑制了癫痫诱导的IGF-1的上调,促进了BDNF的上调,并促进了与细胞增殖、生长、分化和存活相关的ERK和mTOR蛋白的活化。

关键词：癫痫抗阻运动记忆缺陷胰岛素样生长因子1 脑源性神经营养因子