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Biochemical properties of K_(11,48)-branched ubiquitin chains

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Chinese Chemical Letters 2018年第7期29卷 1155-1159页

作者： lu-jun liang Yanyan Si Shan Tang Dongliang Huang Zhipeng A.Wang Changlin Tian Ji-Shen Zheng School of Life Sciences University of Science and Technology of China

As one of the most widely existing post-translational modification models, ubiquitination regulates diverse cellular activities. In eukaryotes, K-branched ubiquitin chains play key roles in cell cycle and protein quality control. However, the structural and biochemical properties of K-branched ubiquitin chains have not been well examined. Here we employed the synthetic linkage-and length-defined K-branched ubiquitin chains to examine their binding and hydrolysis properties in vitro. Quantitatively affinity determination of ubiquitin chains to the proteasome ubiquitin receptor S5 a indicated that the S5 a exhibited preference binding to K-branched chains over K-linked chains, but not K-conjugated chains. In addition, deubiquitination experiments were carried out and the results showed that K-branched chains were preferably hydrolyzed by proteasome-associated deubiquitinase Rpnll than homotypic Kor K-linked chains.

关键词： Post-translational modification Ubiquitination K_(11,48)-branched chains Affinity Deubiquitination Native chemical ligation of peptide hydrazine

Interface-reconstruction Forming Bifunctional(LixTM1-x)O Rock-salt Shell for Enhanced Cyclability in Li-rich Layered Oxide

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Chinese Journal of Structural Chemistry 2020年第8期39卷 1363-1371,1357页

作者： YU Yao-Ming LIU Jia-Jie QI Rui ZUO Chang-Jian ZHAO Wen-Guang lu jun-liang ZHANG Ming-Jian PAN Feng School of Advanced Materials Peking University Shenzhen Graduate SchoolShenzhen 518055China Center for Advanced Radiation Source(ChemMatCARS) the University of ChicagoArgonneIllinois 60439United States

Poor cycling stability,as a long-standing issue,has greatly hindered the commercial application of Li-rich layered oxide cathodes in high-energy-density Li-ion ***-type rock-salt phase is commonly considered electrochemically inert but ***,an ultrathin(LixTM1-x)O rock-salt shell was in situ constructed at the particle surface during the synthesis of Li-rich layered oxide cathodes through a unique soft chemical quenching *** structural/chemical analysis reveals that,it not only inherits the chemical stability of traditional NiO-type rock-salt phase,but also facilitates Li^+diffusion due to the co-occupancy of Li^+and TM *** a bifunctional shell could efficiently prevent TM dissolution and oxygen evolution during the long-term cycling,eventually leading to the enhanced cycling stability for Li-rich layered oxides(92.7%of capacity retention after 200 cycles at 0.5 C).It provides new guidance to design and synthesize new Li-rich layered oxides with the excellent cycling stability through utilizing some electrochemically-inert phases.

关键词： soft chemical quench rock-salt shell core-shell heterostructure Li-rich layered oxide cathodes cycling stability

Ferricyanide-Promoted Oxidative Activation and Ligation of Protein Thioacids in Neutral Aqueous Media

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CCS Chemistry 2024年第8期6卷 2031-2043页

作者： Guo-Chao Chu lu-jun liang Rui Zhao Yan-Yan Guo Chun-Tong Li Chong Zuo Huasong Ai Xiao Hua Zi-Chen Li Yi-Ming Li Lei Liu NewCornerstone Science Laboratory Tsinghua-Peking Center for Life SciencesMinistry of Education Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologyCenter for Synthetic and Systems BiologyDepartment of ChemistryTsinghua UniversityBeijing 100084 School of Food and Biological Engineering Hefei University of TechnologyHefei 230009 Center for BioAnalytical Chemistry Hefei National Laboratory of Physical Science at MicroscaleUniversity of Science and Technology of ChinaHefei 230026 Department of Chemistry University of Science and Technology of ChinaHefei 230026

Ferricyanide-promoted oxidative activation of Nacylatedα-aminothioacids for amide bond formation withα-aminonitriles was recently shown to be a plausible pathway for prebiotic peptide *** we describe the finding that by adding sodium azide and thiols,ferricyanide oxidation can elicit highly efficient and clean conversion of fully unprotected peptide or protein thioacids in neutral aqueous media to the corresponding *** transformation enables the development of ferricyanide-promoted thioacid-based native chemical ligation(NCL)as a new redox-based method for chemical protein synthesis,which does not need to change pH and is therefore operationally easy for ligation at small *** effectiveness of the ferricyanide-promoted thioacid-based NCL was illustrated by synthesis of an ISG15-modified MDA5 segment under nondenaturing conditions and synthesis of an acetylated ubiquitin(Ub)-modified histone H2A through an N-to-C sequential *** work broadens the concept of on-demand oxidative activation strategy for protein ligation and provides a new useful supplement to the repertoire of methods for chemical protein synthesis,particularly for studies on proteins carrying Ub family modifications.

关键词： chemical protein synthesis protein thioacid ferricyanide oxidation native chemical ligation ubiquitin family modifications

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双参量孤子细胞自动机

物理学报 1993年第12期42卷 1895-1900页

作者：陈陆君梁昌洪西安电子科技大学西安710071

提出一种双参量孤子细胞自动机,给出速度的代数表达式及裂变条件,分析了相互作用规律和各种守恒量,引入并阐明了基本孤子概念及其守恒特征的应用。

关键词：细胞自动机孤立子相互作用

Circulating free DNA in the era of precision oncology:Pre-and post-analytical concerns

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Chronic Diseases and Translational Medicine 2016年第4期2卷 -页

作者： jun-liang lu Zhi-Yong liang Department of Pathology Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100730 China

Cancer treatment has entered the era of precision medicine, where knowledge of a patient's genetic profile is used to facilitate early diagnosis, drug selection, prognosis, prediction of drug responsiveness, the onset of secondary resistance, and relapse. Circulating free DNA (cfDNA) has emerged as an ideal source of genetic information for cancer patients, and numerous studies have explored its validity in various clinical applications. However, clinical implementation of cfDNA-based tests has been slow. In this review, we addressed some of the pre-and post-analytical issues regarding cfDNA tests. First, we summarized the charac-teristics of cfDNA and reviewed the methods used to identify tumor-derived cfDNA from the pool of total cfDNA. Second, we described the procedures used to extract cfDNA, which have a great impact on representativeness and yield. Finally, we discussed our thoughts on the validation of cfDNA-based tests and the reporting of test results amid drastic limitations.

关键词： Circulating free DNA Characterization Extraction Validation Interpretation

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基于RS485的船舶货油装卸系统仿真研究

系统仿真学报 2007年第24期19卷 5827-5831页

作者：秦品乐林焰陈明吕均梁大连理工大学船舶CAD中心辽宁大连116023 大连造船重工有限责任公司辽宁大连116005

针对成品油船货油装卸系统自动化研究的关键技术问题,进行了半实物仿真研究。通过对货油装卸工艺的分析,构建了基于RS485总线的由各种硬件设备组成的仿真平台,并应用组态王6.5和VB混合编程技术完成了基于DDE的实时通信程序的开发。为了... 详细信息

针对成品油船货油装卸系统自动化研究的关键技术问题,进行了半实物仿真研究。通过对货油装卸工艺的分析,构建了基于RS485总线的由各种硬件设备组成的仿真平台,并应用组态王6.5和VB混合编程技术完成了基于DDE的实时通信程序的开发。为了减小由液位传感器数据采集延时带来的误差,提出了传感器测量和流量变频控制相结合的改进方案。仿真结果表明:该仿真系统满足货油装卸过程中状态监测及控制的实时要求,对研制实船的货油装卸系统有重要参考价值。

关键词：货油装卸系统组态王6.5 仿真 RS485标准 DDE

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我国旅游业信息化服务模式探讨

商业经济与管理 2005年第6期 61-64页

作者：陆均良朱路平浙江大学浙江杭州310028

信息化服务是现代旅游服务企业的主要特征,本文根据旅游业信息化服务存在的一些问题,特别是针对小规模旅游企业的现状,提出了适合这些企业开展的信息化服务模式框架,即W-C-I模式。

关键词：旅游业信息化模式小规模企业服务

多层螺旋CT在评价门脉海绵样变侧支血管中的应用

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中国医学影像技术 2006年第6期22卷 927-929页

作者：路军良张立华杨宁中国医学科学院中国协和医科大学北京协和医院放射科北京100730

目的利用64层螺旋CT增强扫描和血管重建对门脉海绵样变的解剖关系及侧支血管的形成情况进行评价。方法对我院2004年8月-2005年5月期间收集的18例门脉海绵样变资料进行分析。结果在门脉闭塞后,引起相应血管的纡曲扩张及侧支血管的形成,... 详细信息

目的利用64层螺旋CT增强扫描和血管重建对门脉海绵样变的解剖关系及侧支血管的形成情况进行评价。方法对我院2004年8月-2005年5月期间收集的18例门脉海绵样变资料进行分析。结果在门脉闭塞后,引起相应血管的纡曲扩张及侧支血管的形成,其中有门脉周围形成门门侧支循环,胆囊壁静脉的曲张,胃网膜静脉的增粗纡曲,胆管壁静脉的纡曲扩张,腹膜后椎体旁侧支血管的开放,脾肾静脉短路的开放,胃底食管静脉的曲张。结论多层螺旋CT血管重建在诊断门脉海绵样变方面有重要的临床意义。

关键词：体层摄影术 X线计算机血管造影术海绵样变侧支血管

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中国饭店企业选择网络分销的关键因素研究

财贸研究 2008年第1期19卷 114-120页

作者：陆均良沈华玉浙江大学管理学院浙江杭州310058

从管理者角度对饭店选择网络分销渠道的关键影响因素进行研究,通过前期对饭店管理者和专家访谈以及问卷调查,结合定性分析和定量分析的方法,从八个大的方面总结归纳出26个小的影响因子。在调查研究的基础上,从中提取关键性的因子,得出... 详细信息

从管理者角度对饭店选择网络分销渠道的关键影响因素进行研究,通过前期对饭店管理者和专家访谈以及问卷调查,结合定性分析和定量分析的方法,从八个大的方面总结归纳出26个小的影响因子。在调查研究的基础上,从中提取关键性的因子,得出了影响饭店选择网络分销渠道(中介)的五个关键因素,按重要性顺序排列为:形象基础、效益因素、系统提供商、技术支持和市场因素。为我国饭店企业开展网络分销提供了新的指导和理论视角。

关键词：网络分销中介网络电子商务饭店关键因素