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Neuronal conversion from glia to replenish the lost neurons

Neural Regeneration Research 2024年第7期19卷 1446-1453页

作者： Shiyu Liang Jing zhou Xiaolin Yu Shuai lu Ruitian Liu National Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijingChina School of Chemical Engineering University of Chinese Academy of SciencesBeijingChina Department of Geriatric Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Neuronal injury,aging,and cerebrovascular and neurodegenerative diseases such as cerebral infarction,Alzheimer’s disease,Parkinson’s disease,frontotemporal dementia,amyotrophic lateral sclerosis,and Huntington’s disease are characte rized by significant neuronal *** rtunately,the neurons of most mammals including humans do not possess the ability to *** of lost neurons becomes an appealing therapeutic strategy to reve rse the disease *** of pluripotent neural stem cells can supplement the missing neurons in the brain,but it carries the risk of causing gene mutation,tumorigenesis,severe inflammation,and obstructive hydrocephalus induced by brain *** of neural or non-neural lineage cells into functional neurons is a promising strategy for the diseases involving neuron loss,which may overcome the above-mentioned disadvantages of neural stem cell *** far,many strategies to transfo rm astrocytes,fibroblasts,microglia,Muller glia,ng2 cells,and other glial cells to mature and functional neurons,or for the conversion between neuronal subtypes have been developed thro ugh the regulation of transcription factors,polypyrimidine tra ct binding protein 1(PTBP1),and small chemical molecules or are based on a combination of several factors and the location in the central nervous ***,some recent papers did not obtain expected results,and discrepancies ***,in this review,we discuss the history of neuronal transdifferentiation,summarize the strategies for neuronal replenishment and conversion from glia,especially astrocytes,and point out that biosafety,new strategies,and the accurate origin of the truly co nverted neurons in vivo should be focused upon in future *** also arises the attention of replenishing the lost neurons from glia by gene therapies such as up-regulation of some transc ription factors or downregulation of PTBP1 or drug interfe rence therapies.

关键词： astrocytes neural stem cells neurodegenerative diseases neuron polypyrimidine tract binding protein 1 repair reprogramming small molecule transcription factor transdifferentiation

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基于换流角的桥臂交替导通多电平换流器电容电压控制

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电力系统自动化 2016年第13期40卷 71-76,113页

作者：冯亚东汪楠楠卢宇陈勇清华大学电机工程与应用电子技术系北京市100086 南京南瑞继保电气有限公司江苏省南京市211102

桥臂交替导通多电平换流器(AAMC)的桥臂由多个全桥子模块和带有反向并联二极管的绝缘栅双极型晶体管(IGBT)串联,通过上下桥臂轮流导通来输出多电平的正弦波。如何维持AAMC子模块电容电压稳定是AAMC研究的难点。从桥臂能量平衡的概念出发... 详细信息

桥臂交替导通多电平换流器(AAMC)的桥臂由多个全桥子模块和带有反向并联二极管的绝缘栅双极型晶体管(IGBT)串联,通过上下桥臂轮流导通来输出多电平的正弦波。如何维持AAMC子模块电容电压稳定是AAMC研究的难点。从桥臂能量平衡的概念出发,建立了在特定角度进行上下桥臂换流实现桥臂子模块电容电压控制的能量平衡方程,提出了换流角的计算方法。通过桥臂最大导通电压的研究,提出了两个换流角的解与无功功率的互补特性。依据无功方向优化选择换流角,有效降低了桥臂最大导通电压,减少了桥臂所需子模块的数量。通过换流角与电容电压耦合机理研究,提出了局部线性化的电容电压一阶模型,以此为基础提出了换流角前馈与比例—积分(PI)控制相结合的电容电压控制策略,既提高了控制响应的快速性,又保证了电容电压的稳定性。通过PSCAD/EMTDC仿真验证了基于换流角的子模块电容电压控制方法的有效性。

关键词：电压源换流器型高压直流输电桥臂交替导通多电平换流器能量平衡方程电容电压平衡控制控制策略

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Nitrogen and boron co-doped graphene nanoribbons as peroxidase-mimicking nanozymes for enhanced biosensing

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Chinese Chemical Letters 2022年第1期33卷 344-348页

作者： Siyu luo Meng Sha Fei Tian Xialian Li Lijie Fu Yingqiu Gu lu-lu Qu Guo-Hai Yang Chengzhou zhu School of Chemistry&Materials Science Jiangsu Normal UniversityXuzhou 221116China Key Laboratory of Pesticide and Chemical Biology of Ministry of Education International Joint Research Center for Intelligent Biosensing Technology and HealthCollege of ChemistryCentral China Normal UniversityWuhan 430079China

The rational design of nanozymes with superior activities is essential for improving bioassay ***,nitrogen and boron co-doped graphene nanoribbons(NB-GNRs)are prepared by a hydrothermal method using urea as the nitrogen source and boric acid as the boron source,*** introduction of co-doped and edge structures provides high defects and active *** resultant NB-GNRs nanozymes show superior peroxidase-like activities to nitrogen-doped and boron-doped counterparts due to the synergistic *** taking advantage of their peroxidase-like activities,NB-GNRs are used for the first time to develop enzyme-linked immunosorbent assay for the detection of *** biosensors exhibit a high performance with a linear range from 0.001 ng/mL to 1000 ng/mL and a detection limit of 0.3 pg/*** to their low cost and high stability,the proposed nanomaterials show great promise in biocatalysis,immunoassay development and environmental monitoring.

关键词： Graphene nanoribbons Heteroatom doping Nanozymes Biosensing Interleukin-6

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2型糖尿病患者血清胎盘生长因子水平与颈动脉粥样硬化的相关性研究

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中国糖尿病杂志 2015年第5期23卷 422-425页

作者：张萍成兴波鲁燕苏州大学附属第一医院内分泌科 215006

目的探讨T2DM患者血清胎盘生长因子(PLGF)与颈动脉粥样硬化(CAS)的关系。方法采用ELISA检测T2DM组、T2DM+CAS组及NC组血清PLGF、FPG、血脂、FIns、HbA1c、高敏C反应蛋白(hsC-RP)、TNF-α及IL-6水平,计算BMI和胰岛素抵抗指数(HOMA-IR)。... 详细信息

目的探讨T2DM患者血清胎盘生长因子(PLGF)与颈动脉粥样硬化(CAS)的关系。方法采用ELISA检测T2DM组、T2DM+CAS组及NC组血清PLGF、FPG、血脂、FIns、HbA1c、高敏C反应蛋白(hsC-RP)、TNF-α及IL-6水平,计算BMI和胰岛素抵抗指数(HOMA-IR)。结果T2DM、T2DM+CAS组血清PLGF水平均高于NC组[(45.35±13.00),(59.24±11.05)vs(27.30±10.22)pg/ml](P<0.01)。T2DM+CAS组血清PLGF水平高于T2DM组(P<0.01)。T2DM组血清PLGF水平与TC、LDL-C、hsC-RP、IL-6呈正相关。LDL-C、IL-6是血清PLGF水平的独立影响因素。PLGF是发生CAS风险的预测因素。结论 T2DM合并CAS患者血清PLGF高于正常人和单纯T2DM患者,且与慢性炎症相关。PLGF可能参与了T2DM患者CAS的发生发展。

关键词：糖尿病 2型胎盘生长因子颈动脉粥样硬化

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抗丁香酚单克隆抗体的研制及胶体金试纸条检测方法的建立

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核农学报 2025年第2期39卷 339-350页

作者：董晓涵翟璐黄雅丽杨加成陈青舟廖杰金仁耀浙江工商大学海洋食品研究院浙江杭州310012 杭州博岳生物技术有限公司浙江杭州310018 杭州医学院公共卫生学院浙江杭州310013 杭州南开日新生物技术有限公司浙江杭州310000 浙江华才检测技术有限公司浙江绍兴311800

为制备高特异性和高灵敏度的抗丁香酚(Eugenol)单克隆抗体,建立检测丁香酚的胶体金免疫层析法,本研究采用亲核取代法合成丁香酚半抗原(Eugenol-COOH)并进行质谱(HRMS)鉴定;采用碳二亚胺法制备丁香酚免疫抗原(Eugenol-BSA)和包被抗原(Eug... 详细信息

为制备高特异性和高灵敏度的抗丁香酚(Eugenol)单克隆抗体,建立检测丁香酚的胶体金免疫层析法,本研究采用亲核取代法合成丁香酚半抗原(Eugenol-COOH)并进行质谱(HRMS)鉴定;采用碳二亚胺法制备丁香酚免疫抗原(Eugenol-BSA)和包被抗原(Eugenol-OVA),进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和紫外扫描鉴定;利用免疫抗原Eugenol-BSA免疫小鼠,经细胞融合、筛选和克隆,最终得到1株能稳定分泌抗丁香酚单克隆抗体的杂交瘤细胞株(3D9),并准备腹水,利用间接竞争ELISA(icELISA)方法测定腹水效价;对获得的单克隆抗体进行鉴定,经条件优化建立胶体金免疫层析试纸条检测方法,对试纸条的灵敏度和稳定性进行评价。结果表明,Eugenol-BSA偶联比约为16∶1,Eugenol-OVA偶联比约为10∶1,完全抗原偶联成功;3D9细胞株的抗体类型及亚类均为免疫球蛋白G(IgG1),轻链为κ链;单克隆抗体效价达1×10^(-6);丁香酚浓度与抑制率之间的线性回归方程为y=9.671lnx+10.539(R^(2)=0.998),半数抑制浓度(IC_(50))为59.2 ng·mL^(-1),表明制备的抗丁香酚单克隆抗体具有较高的检测灵敏度。所制备的胶体金免疫试纸条优化参数为:胶体金标记最适pH值9、0.2 mol·L^(-1)K2CO3添加量12μL、抗体最佳标记量7μg·mL^(-1)、胶体金试纸条的T线消失浓度1.8μg·mL^(-1),该参数下的裸眼检出限0.3μg·mL^(-1)。本研究可为丁香酚的现场检测提供有效方法。

关键词：丁香酚单克隆抗体人工抗原胶体金试纸条

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Room-temperature multiple ligands-tailored SnO_(2) quantum dots endow in situ dual-interface binding for upscaling efficient perovskite photovoltaics with high V_(OC)

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Light(Science & Applications) 2021年第12期10卷 2467-2481页

作者： zhiwei Ren Kuan Liu Hanlin Hu Xuyun Guo Yajun Gao Patrick W.K.Fong Qiong Liang Hua Tang Jiaming Huang Hengkai zh.ng Minchao Qin Li Cui Hrisheekesh Thachoth Chandran Dong Shen Ming-Fai Lo Annie ng Charles Surya Minhua Shao Chun-Sing Lee Xinhui lu Frédéric Laquai Ye zhu Gang Li Department of Electronic and Information Engineering Research Institute for Smart Energy(RISE)Guangdong-Hong Kong-Macao(GHM)Joint Laboratory for Photonic-Thermal-Electrical Energy Materials and DevicesThe Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina Department of Electrical and Computer Engineering Nazarbayev UniversityNur-SultanKazakhstan The Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen 518057China Hoffmann Institute of Advanced Materials Shenzhen Polytechnic7098 Liuxian BoulevardShenzhen 518055China Department of Applied Physics The Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina Department of Chemical and Biological Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina King Abdullah University of Science and Technology(KAUST) KAUST Solar Center(KSC)Physical Sciences and Engineering Division(PSE)Material Science and Engineering Program(MSE)Thuwal 23955-6900Kingdom of Saudi Arabia Department of Physics The Chinese University of Hong KongShatin 999077 Hong KongChina Center of Super-Diamond and Advanced Films(COSDAF) Department of ChemistryCity University of Hong KongHong KongChina

The benchmark tin oxide(SnO_(2))electron transporting layers(ETLs)have enabled remarkable progress in planar perovskite solar cell(PSCs).However,the energy loss is still a challenge due to the lack of“hidden interface”*** report a novel ligand-tailored ultrafine SnO_(2) quantum dots(QDs)via a facile rapid room temperature ***,the ligand-tailored SnO_(2) QDs ETL with multi-functional terminal groups in situ refines the buried interfaces with both the perovskite and transparent electrode via enhanced interface binding and perovskite *** novel ETLs induce synergistic effects of physical and chemical interfacial modulation and preferred perovskite crystallization-directing,delivering reduced interface defects,suppressed non-radiative recombination and elongated charge carrier *** conversion efficiency(PCE)of 23.02%(0.04 cm^(2))and 21.6%(0.98 cm^(2),V_(OC) loss:0.336 V)have been achieved for the blade-coated PSCs(1.54 eV E_(g))with our new ETLs,representing a record for SnO_(2) based blade-coated ***,a substantially enhanced PCE(V_(OC))from 20.4%(1.15 V)to 22.8%(1.24 V,90 mV higher V_(OC),0.04 cm^(2) device)in the blade-coated 1.61 eV PSCs system,via replacing the benchmark commercial colloidal SnO_(2) with our new ETLs.

关键词： perovskite interface quantum

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CD4^+CD45RB^(high)T细胞转入SCID鼠诱导的结肠炎模型

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世界华人消化杂志 2005年第24期13卷 2866-2869页

作者：陆丽华冉志华上海交通大学医学院附属仁济医院上海市消化疾病研究所上海市200001

炎症性肠病的病因未明,因此此疾病的动物模型的制备比较困难.现有的多种制模方法都不能完全模拟人的炎症性肠病.我们介绍一种比较新的免疫方法诱导的结肠炎动物模型,即用CD4+CD45RBhighT细胞转入SCID鼠诱导产生结肠炎.以下就此模型的制... 详细信息

炎症性肠病的病因未明,因此此疾病的动物模型的制备比较困难.现有的多种制模方法都不能完全模拟人的炎症性肠病.我们介绍一种比较新的免疫方法诱导的结肠炎动物模型,即用CD4+CD45RBhighT细胞转入SCID鼠诱导产生结肠炎.以下就此模型的制备方法、原理、特点、与IBD的相似处、用途、优缺点作一综述.

关键词： β-catenin Wnt信号通路消化道肿瘤

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Potential involvement of nitric oxide synthase but not inducible nitric oxide synthase in the development of experimental corneal neovascularization

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International Journal of Ophthalmology(English edition) 2011年第4期4卷 343-348页

作者： Yuan Chen Gao-Qin Liu and Pei-Rong lu Department of Ophthalmology the First Affiliated Hospital of Soochow University Suzhou 215006 Jiangsu Province China

AIM: To investigate the effect of nitric oxide and its synthetase on experimental corneal neovascularization (CRNV). METHODS: CRNV was induced by alkali injury in mice, nitric oxide synthetase (NOS) was inhibited by ng-nitro-L-arginine (L-NAME) and inducible nitric oxide synthetase (iNOS) was inhibited by aminoguanidine hemisulfate salt (AG). The inhibitory effect was detected at day 2 and 4 after corneal alkali injury by reverse transcription polymerase chain reaction (RT-PCR). CRNV was compared between the control and the treated mice by microscopic observation and corneal whole mount CD31 immunostaining. RESULTS: The inhibition of L-NAME to NOS and AG to iNOS after corneal injury was confirmed by RT-PCR (P 0.05). CONCluSION: Our findings suggest that NOS but not iNOS plays a critical role in alkali injury induced CRNV.

关键词： corneal neovascularization nitric oxide synthase inducible nitric oxide synthase

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Effect of propofol on generation of inflammatory mediator of monocytes

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Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 940-945页

作者： Yun Nie Yan-Xi lu Li-Hong Lv Operating Room First People's Hospital of Ji'nan

Objective:To evaluate the effect of propofol with different concentrations on the expression of inflammatory mediators of mterleukin and tumor-necrosis factor- α(TNF- α) by stimulating the mouse primary monocytes and human monocytic cell line with lipopolysaccharide(LPS)and also discuss the effeet of propofol on the secretion of inflammatory mediator and its possible molecular ***:The mononuclear cells of mouse spleen were separated and then purified to obtain the primary *** dose-effect relationship of production of pro-inflammatory cytokines by monocytes which were stimulated by LPS,namely the monocytes were stimulated by the dose of 0-500 ng/mL for 24 *** was employed to detect the concentration of IL-6,TL-8 and TNF-α.The effect of propofol on the secretion of above pro-inflammatory cytokines by the monocytes was *** were divided into the control group,the 0.1%DMSO group,the LPS group and the treatment group with LPS + different dose of propofol(propofol 1-100 μg/mL).ELISA was employed to detect the concentration of IL-6,IL-8 and TNF-α.The change in the expression of important signaling molecules in Toll-like receptor and NF-κ B signaling pathway was detected after THP-1 cells were treated with ***:The concentration of TNF- α was(3 863±153)pg/mL after 12 h of stimulation by LPS and then its concentration was decreased *** the concentration of IL-6 and IL-8 was relatively high after 24 h of stimulation by LPS,(5 627±330) pg/mL and(1 626±200) pg/mL,*** propofol could inhibit the expression of IL-6,IL-8 and TNF- α caused by *** the intervention treatment of 50 μg/mL propofol,the concentration of IL-6,IL-8 and TNF-α was significantly decreased(Plusions:The propofol can inhibit the expression of TLR-4 and NF-κ B to inhibit the activation of p38 and the expression of pro-inflammatory cytokines.

关键词： Propofol LPS IL-6 IL-8 TNF-α Anti-inflammation

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A capacitive sensor based on molecularly imprinted polymers and poly(p-aminobenzene sulfonic acid) film for detection of pazufloxacin mesilate

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Science China Chemistry 2007年第4期50卷 547-553页

作者： zhOU lu YE GuangRong YUAN Ruo CHAI YaQin CHEN SuMing College of Chemistry and Chemical Engineering Southwest UniversityChongqing 400715China

A novel capacitive sensor for pazufloxacin mesilate (pazufloxacin) determination was developed by electropolymerizing p-aminobenzene sulfonic (p-ABSA) and molecularly imprinted polymers (MPs), which was synthesized through thermal radical copolymerization of metharylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of pazufloxacin template molecules, on the gold electrode surface. Furthermore, 1-dedecanethiol was used to insulate the modified electrode. Alternating current (ac) impedance experiments were carried out with a Model IM6e to obtain the capacitance responses. Under the optimum conditions, the sensor showed linear capacitance response to pazufloxacin in the range of 5 ng·mL?1 to 5 μg·mL?1 with a relative standard deviation (RSD) 5.3% (n=7) and a detection limit of 1.8 ng·mL?1. The recoveries for different concentration levels of pazufloxacin samples varied from 94.0% to 102.0%. Electrochemical experiments indicated the capacitive sensor exhibited good sensitivity and selectivity and showed excellent parameters of regeneration and stability.

关键词： capacitive sensor molecularly imprinted polymers p-aminobenzene sulfonic acid pazufloxacin mesilate

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