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Natural killer cells are activated and play a protective role against ZIKA virus infection in mice

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Science Bulletin 2017年第14期62卷 982-984页

作者： Xuefeng Duan Shihua Li Gary Wong Dongfang Wang Haoyu Wang Jiao lu Yuhai Bi xuancheng lu Yi Shi Jinghua Yan Min Fang George Fu Gao CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences University of Chinese Academy of Sciences Institute of Health Sciences Anhui University Laboratory Animal Center Chinese Center for Disease Control and Prevention (China CDC) International College University of Chinese Academy of Sciences National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention (China CDC) Savaid Medical School University of Chinese Academy of Sciences Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of Sciences

Zika virus(ZIKV)is a mosquito-borne Flavivirus that was responsible for an epidemic in the Americas during 2015–2016[1].ZIKV infections in adults have been linked to Guillain-Barrésyndrome[2,3],as well as abnormalities such as abortion,stillbirth,and microcephaly in some cases[4].In addition,we have reported that ZIKV infect testicular stem-like peritubular

关键词：病毒感染细胞活性保护作用杀伤自然小鼠对齐

Establishment of a humanized ST6GAL1 mouse model for influenza research

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Animal Models and Experimental Medicine 2024年第3期7卷 337-346页

作者： Lyu Chao Han Feng Gao Qian Lv Limin lu Ziwei lu Shuangshuang Li Xiaoyan Hu Yuechao Yang Mengjie Zhao Yingze Liu Jun lu xuancheng Duo Shuguang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Institute of Zoology Chinese Academy of SciencesBeijingChina NHC Key Laboratory of Biosafety National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Zhejiang Provincial Key Laboratory of Medical Genetics Key Laboratory of Laboratory MedicineMinistry of EducationSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhouChina

Background:This study aimed to construct and characterize a humanized influenza mouse model expressing ***:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of *** manipulated embryos were transferred into the oviducts of pseudopregnant female *** offspring were identified using *** exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for *** of the humanized gene was tested by performing immunohistochemical(IHC)*** and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were ***:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT *** mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection *** IHC results revealed that hST6GAL1 was expressed in the lungs of humanized ***,the hematologic and biochemical parameters of the positive mice were within the normal ***:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.

关键词： hST6GAL1 humanized mice influenza animal model

Anti-infection effects of heparin on SARS-CoV-2 in a diabetic mouse model

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Zoological Research 2023年第6期44卷 1003-1014页

作者： Zhongyun Zhang Ning Zhang xuancheng lu Min Zhou Xiaoxiang Yan Weiqiong Gu Jingru Yang Qin Zhang Cheng Zhang Yuhuan Gong Mingjun Jia Xiaoyu Zhang Peng Ning Mei Liu Xiaoyan Li Xiaomeng Shi Wenjun Liu George FGao Guang Ning Jiqiu Wang Yuhai Bi Department of Endocrinology and Metabolism Shanghai Institute of Endocrine and Metabolic DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai National Clinical Research Center for Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR ChinaShanghai National Center for Translational MedicineShanghai 200025China CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning(CASCIRE)CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID)Chinese Academy of SciencesBeijing 100101China Laboratory Animal Center Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China Department of Respiratory and Critical Care Medicine Institute of Respiratory DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Cardiology Institute of Cardiovascular DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China School of Laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhouZhejiang 325000China University of Chinese Academy of Sciences Beijing 100049China

Severe acute respiratory syndrome coronavirus 2(SARSCo V-2)infection can result in more severe syndromes and poorer outcomes in patients with diabetes and ***,the precise mechanisms responsible for the combined impact of coronavirus disease 2019(COVID-19)and diabetes have not yet been elucidated,and effective treatment options for SARS-Co V-2-infected diabetic patients remain *** investigate the disease pathogenesis,K18-h ACE2 transgenic(h ACE2^(Tg))mice with a leptin receptor deficiency(h ACE2-Lepr^(-/-))and high-fat diet(h ACE2-HFD)background were *** two mouse models were intranasally infected with a 5×10^(5) median tissue culture infectious dose(TCID_(50))of SARSCo V-2,with serum and lung tissue samples collected at 3days *** h ACE2-Lepr^(-/-)mice were then administered a combination of low-molecular-weight heparin(LMWH)(1 mg/kg or 5 mg/kg)and insulin via subcutaneous injection prior to intranasal infection with1×10^(4) TCID_(50)of SARS-Co *** drug administration continued until the euthanasia of the *** of viral RNA loads,histopathological changes in lung tissue,and inflammation factors were *** demonstrated similar SARS-Co V-2 susceptibility in h ACE2^(Tg)mice under both lean(chow diet)and obese(HFD)***,compared to the h ACE2-Lepr^(+/+)mice,h ACE2-Lepr^(-/-)mice exhibited more severe lung injury,enhanced expression of inflammatory cytokines and hypoxia-inducible factor-1α(HIF-1α),and increased ***,combined LMWH and insulin treatment effectively reduced disease progression and severity,attenuated lung pathological changes,and mitigated inflammatory *** conclusion,preexisting diabetes can lead to more severe lung damage upon SARS-Co V-2 infection,and LMWH may be a valuable therapeutic approach for managing COVID-19patients with diabetes.

关键词： SARS-CoV-2 Diabetes Mouse model Heparin Antiviral therapy

生物安全二级动物实验设施(ABSL-2)运行管理要点分析

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中国比较医学杂志 2019年第7期29卷 80-85页

作者：李晓燕卢选成中国疾病预防控制中心实验动物中心

生物安全二级动物实验设施(ABSL-2)是可以开展感染性动物实验的生物安全实验室,也是目前科研院所使用频率较高的动物实验设施,设施的有效管理是开展动物实验的前提和保证。笔者以所在单位的ABSL-2设施运行管理为参考,总结归纳该类设施... 详细信息

生物安全二级动物实验设施(ABSL-2)是可以开展感染性动物实验的生物安全实验室,也是目前科研院所使用频率较高的动物实验设施,设施的有效管理是开展动物实验的前提和保证。笔者以所在单位的ABSL-2设施运行管理为参考,总结归纳该类设施在日常运行管理中的几个重点环节,以期为同行提供有价值的参考。

关键词：实验动物生物安全二级动物实验设施运行管理

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仿生扑翼无人系统研究综述

智能系统学报 2023年第3期18卷 410-439页

作者：王军张震李富强卢宣成中国矿业大学信息与控制工程学院江苏徐州221000

仿生扑翼无人系统是模仿自然界中蝙蝠、鸟类和昆虫的飞行方式研发的一种新型无人系统,可在高度复杂和动态的环境中飞行,并对环境的干扰具有良好的鲁棒性,是机器人领域未来发展的重要研究方向之一。本文对近年来国内外扑翼无人系统方面... 详细信息

仿生扑翼无人系统是模仿自然界中蝙蝠、鸟类和昆虫的飞行方式研发的一种新型无人系统,可在高度复杂和动态的环境中飞行,并对环境的干扰具有良好的鲁棒性,是机器人领域未来发展的重要研究方向之一。本文对近年来国内外扑翼无人系统方面的相关研究进行综述,分别从空气动力学、系统识别与建模、感知和控制等几个方面介绍了扑翼无人系统的最新进展,最后提炼出扑翼无人系统的各个方面的挑战,并对该领域未来的发展和研究方向做出了展望。

关键词：扑翼无人系统空气动力学数值模拟系统识别建模系统感知姿态控制振动控制

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若尔盖高寒湿地-大气间水热交换湍流通量的日变化特征分析

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高原气象 2020年第4期39卷 719-728页

作者：陆宣承文军田辉杨越杨奥莉成都信息工程大学大气科学学院/高原大气与环境四川省重点实验室四川成都610225 中国科学院西北生态环境与资源研究院/中国科学院寒旱区陆面过程与气候变化重点实验室甘肃兰州730000

湿地近地面水热交换对陆面过程乃至天气气候变化有着显著影响,准确量化湿地与大气间的水热交换通量具有重要意义。本文利用中国科学院西北生态环境与资源研究院若尔盖花湖湿地陆面过程与气候变化观测场(下称花湖观测场)2017年3月至2018... 详细信息

湿地近地面水热交换对陆面过程乃至天气气候变化有着显著影响,准确量化湿地与大气间的水热交换通量具有重要意义。本文利用中国科学院西北生态环境与资源研究院若尔盖花湖湿地陆面过程与气候变化观测场(下称花湖观测场)2017年3月至2018年3月涡动相关系统的观测数据,各季节选取3个典型晴天,分析了若尔盖湿地近地面的感热通量和潜热通量日变化特征,并与鄂陵湖和玛曲草原的观测值进行对比,同时计算了湿地下垫面的能量闭合率。结果表明:若尔盖高寒湿地-大气间的水热交换过程存在着明显的日变化特征。感热通量和潜热通量的日变化过程都为单峰型,在14:00(北京时,下同)-15:00达到最大值,感热通量最大值可达101.7 W·m^-2。潜热通量最大值可达412.6 W·m^-2。寒冷干燥季节的感热通量日平均值比温暖湿润季节大18.0%;而温暖潮湿季节潜热通量日平均值比寒冷干燥季节高68.7%。本文还将湿地水热交换过程与玛曲草原以及鄂陵湖湖面的观测数据进行了对比发现:夏季,若尔盖湿地近地面与鄂陵湖湖面向大气输送的感热和潜热总量相当,但湿地日变化幅度远大于湖面,通常为湖面的4~7倍。玛曲草原夏季感热通量日变幅约为若尔盖湿地的1.5倍,而湿地夏季潜热通量总量约为草原的1.2倍。在地表向上的通量中,能量不平衡所占的比例:春季27.7%,夏季22.7%,秋季15.7%,冬季19.4%。湿地全年主要以潜热的形式向大气输送能量,夏季潜热通量占有效能量的比例可达58.0%。

关键词：若尔盖高寒湿地水热交换通量日变化

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教学中动物实验替代方法应用进展

中国比较医学杂志 2020年第7期30卷 133-138页

作者：蒋亚君荣蓉刘晓宇卢选成中国疾病预防控制中心实验动物中心北京102206

1959年英国动物学家***和微生物学家***提出替代、减少和优化的“3R原则”。其中替代是开展动物实验时首要考虑的原则。本文综述了国际和我国教学中实验动物替代方法应用现状,对目前可用替代方法包括模型、道具和机械模拟器;胶片和视频... 详细信息

1959年英国动物学家***和微生物学家***提出替代、减少和优化的“3R原则”。其中替代是开展动物实验时首要考虑的原则。本文综述了国际和我国教学中实验动物替代方法应用现状,对目前可用替代方法包括模型、道具和机械模拟器;胶片和视频教学;虚拟仿真实验系统以及3D打印技术等进行了介绍,对于如何在我国切实推动教学领域动物替代方法,提出了须提高重视、储备技术以及保证经费等建议。

关键词：教学实验动物实验动物替代实验动物福利

多重耐药纹带棒杆菌BALB/c小鼠肺部感染模型的建立和评估

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中华微生物学和免疫学杂志 2020年第12期40卷 941-949页

作者：柴媛王艳艳卢选成英兰吕莹莹王俊瑞内蒙古医科大学第一临床医学院呼和浩特010050 内蒙古医科大学附属医院检验科呼和浩特010050 中国疾病预防控制中心动物中心北京102206 内蒙古自治区第四医院病理科呼和浩特010110

目的建立多重耐药纹带棒杆菌小鼠肺部感染模型,对感染小鼠肺部病理改变及炎性指标变化进行评价,为更好认识纹带棒杆菌致病性提供实验依据。方法采用腹腔注射环磷酰胺的方法构建BALB/c小鼠免疫抑制状态,利用超声雾化方法将纹带棒杆菌混悬... 详细信息

目的建立多重耐药纹带棒杆菌小鼠肺部感染模型,对感染小鼠肺部病理改变及炎性指标变化进行评价,为更好认识纹带棒杆菌致病性提供实验依据。方法采用腹腔注射环磷酰胺的方法构建BALB/c小鼠免疫抑制状态,利用超声雾化方法将纹带棒杆菌混悬液(高浓度组:109 CFU/ml;低浓度组:108 CFU/ml)感染BALB/c小鼠。分别在感染后24、48、72和96 h观察小鼠状态,检测小鼠肺组织病理学改变、肺组织和支气管肺泡灌洗液(BALF)中细菌菌落计数及血清IL-6、TNF-α水平的变化。结果实验组小鼠于感染纹带棒杆菌后状态变差、体重下降。感染后24和48 h,小鼠肺组织外观呈明显充血、水肿状态;肺组织HE染色结果显示肺部炎性改变程度随感染时间延长不断加重,感染后96 h小鼠肺间质重度充血,大量炎细胞浸润。与低浓度组小鼠相比,各感染时间点高浓度组小鼠肺组织炎性改变程度更为严重。小鼠外周血白细胞及中性粒细胞计数在感染后24 h开始增高,96 h达到峰值。感染后24 h,肺组织及BALF中纹带棒杆菌大量增殖,并且高浓度组小鼠BALF中菌落计数明显高于低浓度组[(15.13±0.70)×105 CFU/ml vs(30.83±1.10)×105 CFU/ml,F=1253.498,P=0.000]。与对照组相比,实验组小鼠各感染时间点血清IL-6水平均高于对照组,但高低浓度组实验小鼠TNF-α水平变化趋势不完全一致。结论本研究成功构建了纹带棒杆菌BALB/c小鼠肺部感染模型。免疫抑制小鼠在纹带棒杆菌攻击下呈现典型肺部感染病理表现。临床样本特别是下呼吸道标本中分离到多重耐药纹带棒杆菌时应给予更多关注。

关键词：纹带棒杆菌动物模型肺部感染

Understanding the local structure and thermophysical behavior of Mg-La liquid alloys via machine learning potential

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 439-449页

作者： Jia Zhao Taixi Feng Guimin lu National Engineering Research Center for Integrated Utilization of Salt Lake Resource East China University of Science and TechnologyShanghai 200237China Joint International Laboratory for Potassium and Lithium Strategic Resources East China University of Science and TechnologyShanghai 200237China

The local structure and thermophysical behavior of Mg-La liquid alloys were in-depth understood using deep potential molecular dynamic(DPMD) simulation driven via machine learning to promote the development of Mg-La alloys. The robustness of the trained deep potential(DP) model was thoroughly evaluated through several aspects, including root-mean-square errors(RMSEs), energy and force data, and structural information comparison results;the results indicate the carefully trained DP model is reliable. The component and temperature dependence of the local structure in the Mg-La liquid alloy was analyzed. The effect of Mg content in the system on the first coordination shell of the atomic pairs is the same as that of temperature. The pre-peak demonstrated in the structure factor indicates the presence of a medium-range ordered structure in the Mg-La liquid alloy, which is particularly pronounced in the 80at% Mg system and disappears at elevated temperatures. The density, self-diffusion coefficient, and shear viscosity for the Mg-La liquid alloy were predicted via DPMD simulation, the evolution patterns with Mg content and temperature were subsequently discussed, and a database was established accordingly. Finally, the mixing enthalpy and elemental activity of the Mg-La liquid alloy at 1200 K were reliably evaluated,which provides new guidance for related studies.

关键词： magnesium-lanthanum liquid alloys local structure macroscopic properties thermodynamic behavior deep potential mo-lecular dynamic simulation