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Establishment of a humanized ST6GAL1 mouse model for influenza research

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Animal Models and Experimental Medicine 2024年第3期7卷 337-346页

作者： Lyu Chao Han Feng Gao Qian Lv Limin lu Ziwei lu Shuangshuang Li Xiaoyan Hu Yuechao Yang Mengjie Zhao Yingze Liu Jun lu xuancheng Duo Shuguang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Institute of Zoology Chinese Academy of SciencesBeijingChina NHC Key Laboratory of Biosafety National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Zhejiang Provincial Key Laboratory of Medical Genetics Key Laboratory of Laboratory MedicineMinistry of EducationSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhouChina

Background:This study aimed to construct and characterize a humanized influenza mouse model expressing ***:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of *** manipulated embryos were transferred into the oviducts of pseudopregnant female *** offspring were identified using *** exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for *** of the humanized gene was tested by performing immunohistochemical(IHC)*** and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were ***:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT *** mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection *** IHC results revealed that hST6GAL1 was expressed in the lungs of humanized ***,the hematologic and biochemical parameters of the positive mice were within the normal ***:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.

关键词： hST6GAL1 humanized mice influenza animal model

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洪涝灾害下新型配电网安全风险评估方法设计

自动化与仪器仪表 2024年第3期 140-143,148页

作者：赵绪亮谭华章陆炫成胡德其广西电网有限责任公司柳州供电局广西柳州545005

当前对于洪涝灾害条件下的配电网安全风险评估方法主要采用云模型计算,该方法易受到系统电压越限的影响,使得评估准确率较低。沃茨。提出洪涝灾害下新型配电网安全风险评估方法设计。从洪涝灾害前、灾中和灾后三个角度选取能够表征电网... 详细信息

当前对于洪涝灾害条件下的配电网安全风险评估方法主要采用云模型计算,该方法易受到系统电压越限的影响,使得评估准确率较低。沃茨。提出洪涝灾害下新型配电网安全风险评估方法设计。从洪涝灾害前、灾中和灾后三个角度选取能够表征电网系统运行状态的风险评估指标,建立评估指标体系,在静态与动态两个维度计算各指标的隶属度函数,根据评判集与指标集确定指标综合权重,以此为依据,结合指标可信度原理,计算系统电压越限概率,进而求取配电网风险值,明确安全风险等级。仿真实验结果表明,所提方法对于洪涝灾害下的配电网安全风险评估具有更高的评估准确率。

关键词：洪涝灾害新型配电网安全风险风险评估

动物生物安全三级模拟实验室的建设与实践探讨

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中国比较医学杂志 2024年第6期34卷 87-92页

作者：再那吾东·玉山李晓燕鹿双双王嘉琪卡力比夏提·艾木拉江刘美刘科亮卢选成传染病溯源预警与智能决策全国重点实验室中国疾病预防控制中心北京102206 省部共建中亚高发病因与防治国家重点实验室新疆医科大学第一附属医院临床医学研究院乌鲁木齐830054 四川省疾病预防控制中心成都610044

动物生物安全三级实验室(ABSL-3实验室)是可以开展高致病性病原微生物感染实验动物的生物安全防护水平为三级的高级别生物安全实验室。近年来随着新发、再发传染病的不断出现,高级别生物安全实验室在病原致病机理、药物和疫苗研发等方... 详细信息

动物生物安全三级实验室(ABSL-3实验室)是可以开展高致病性病原微生物感染实验动物的生物安全防护水平为三级的高级别生物安全实验室。近年来随着新发、再发传染病的不断出现,高级别生物安全实验室在病原致病机理、药物和疫苗研发等方面发挥了越来越重要的硬件支撑作用,ABSL-3实验室的需求与日俱增。与此同时,对于能够胜任ABSL-3实验室工作的人员需求也越来越多,岗前培训的系统化、规范化、标准化成为进入ABSL-3实验室开展工作的人员生物安全的重要保证。ABSL-3实验室的岗前培训工作需要在特定的场所——模拟实验室内开展,因此有必要建立ABSL-3模拟实验室,并建立科学有效的运行标准和机制。本文全面介绍了ABSL-3模拟实验室的设计、建设、运行及作用。

关键词： ABSL-3实验室 ABSL-3模拟实验室实验室获得性感染生物安全风险评估培训

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深埋地下传感器无线通信小波去噪方法

自动化与仪器仪表 2024年第7期 205-207,212页

作者：赵绪亮吉祥韦劼谭华章陆炫成郑杰广西电网有限责任公司柳州供电局广西柳州545005

深埋地下的传感器组网比正常环境复杂,信号在信道离散时会出现码间干扰,导致传感输出信噪比较低,处于高噪声干扰状态。提出基于小波变换的深埋地下传感器无线通信去噪方法。利用小波变换算法对深埋底下的传感信号离散化,抑制码间干扰情... 详细信息

深埋地下的传感器组网比正常环境复杂,信号在信道离散时会出现码间干扰,导致传感输出信噪比较低,处于高噪声干扰状态。提出基于小波变换的深埋地下传感器无线通信去噪方法。利用小波变换算法对深埋底下的传感信号离散化,抑制码间干扰情况,对通信数据去重以及融合处理,并对通讯协议的数据帧头格式设计,建立无连接的双向通讯协议,完成无线通信。在实验中,以深埋地下传感器为基础,对提出的方法进行了抗噪声性能的检验。经实验结果表明:采用提出的方法进行无线通信时,输出信噪比较高,具备较好的抗噪声性能。

关键词：深埋地下小波变换抗噪声传感器融合处理无线通信

《动物研究:体内实验报告》即ARRIVE 2.0指南的解释和阐述(五)

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实验动物与比较医学 2024年第1期44卷 105-114页

作者：马政文李夏莹刘晓宇李垚王剑卢今陈国元卢晓白玉卢选成刘永刚陶雨风庞万勇上海交通大学医学院实验动物科学部上海200025 北京大学生命科学学院实验动物中心北京100871 中国疾病预防控制中心职业卫生与中毒控制所北京100050 中国科学院分子细胞科学卓越创新中心动物实验技术平台上海200031 迪哲医药股份有限公司上海201203 北京诺和诺德医药科技有限公司北京102206 中国疾病预防控制中心传染病溯源预警与智能决策全国重点实验室北京102206 中国农业科学院哈尔滨兽医研究所哈尔滨150001 中国合格评定国家认可中心北京100062 赛诺菲公司全球研发中心转化体内模型研究平台北京100022

提高生物医学研究结果的可重复性是一项重大挑战,研究人员透明且准确地报告其研究过程有利于读者对该研究结果的可靠性进行评估,进而重复该实验或在该成果的基础上进一步探索。ARRIVE 2.0指南是英国国家3Rs中心(NC3Rs)于2019年组织发布... 详细信息

提高生物医学研究结果的可重复性是一项重大挑战,研究人员透明且准确地报告其研究过程有利于读者对该研究结果的可靠性进行评估,进而重复该实验或在该成果的基础上进一步探索。ARRIVE 2.0指南是英国国家3Rs中心(NC3Rs)于2019年组织发布的一份适用于任何与活体动物研究报告相关的指导性清单,用以提高动物体内实验设计、实验实施和实验报告的规范性,以及动物实验结果的可靠性、可重复性和临床转化率。ARRIVE 2.0指南的使用不仅可以丰富动物实验研究报告的细节,确保动物实验结果信息被充分评估和利用,还可以使读者准确且清晰地了解作者所表述的内容,促进基础研究评审过程的透明化和完整性。本文是在国际期刊遵循ARRIVE 2.0指南的最佳实践基础上,对2020年发表于PLoS Biology期刊上的ARRIVE 2.0指南完整解读版(https://***)第五部分包括“推荐11条”里的第6~11条:“动物照护和监测”、“解析/科学阐释”、“可推广性/转化”、“研究方案注册”、“数据获取”和“利益冲突声明”等内容进行了编译、解释和阐述,以期促进国内研究人员充分理解并使用ARRIVE 2.0指南,提高实验动物研究及报告的规范性,助推我国实验动物科技与比较医学研究的高质量发展。

关键词：动物实验 ARRIVE 2.0指南 ARRIVE推荐11条疼痛管理动物照护和监测

Natural killer cells are activated and play a protective role against ZIKA virus infection in mice

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Science Bulletin 2017年第14期62卷 982-984页

作者： Xuefeng Duan Shihua Li Gary Wong Dongfang Wang Haoyu Wang Jiao lu Yuhai Bi xuancheng lu Yi Shi Jinghua Yan Min Fang George Fu Gao CAS Key Laboratory of Pathogenic Microbiology and Immunology Institute of Microbiology Chinese Academy of Sciences University of Chinese Academy of Sciences Institute of Health Sciences Anhui University Laboratory Animal Center Chinese Center for Disease Control and Prevention (China CDC) International College University of Chinese Academy of Sciences National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention (China CDC) Savaid Medical School University of Chinese Academy of Sciences Research Network of Immunity and Health (RNIH) Beijing Institutes of Life Science Chinese Academy of Sciences

Zika virus(ZIKV)is a mosquito-borne Flavivirus that was responsible for an epidemic in the Americas during 2015–2016[1].ZIKV infections in adults have been linked to Guillain-Barrésyndrome[2,3],as well as abnormalities such as abortion,stillbirth,and microcephaly in some cases[4].In addition,we have reported that ZIKV infect testicular stem-like peritubular

关键词：病毒感染细胞活性保护作用杀伤自然小鼠对齐

Anti-infection effects of heparin on SARS-CoV-2 in a diabetic mouse model

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Zoological Research 2023年第6期44卷 1003-1014页

作者： Zhongyun Zhang Ning Zhang xuancheng lu Min Zhou Xiaoxiang Yan Weiqiong Gu Jingru Yang Qin Zhang Cheng Zhang Yuhuan Gong Mingjun Jia Xiaoyu Zhang Peng Ning Mei Liu Xiaoyan Li Xiaomeng Shi Wenjun Liu George FGao Guang Ning Jiqiu Wang Yuhai Bi Department of Endocrinology and Metabolism Shanghai Institute of Endocrine and Metabolic DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Shanghai National Clinical Research Center for Metabolic Diseases Key Laboratory for Endocrine and Metabolic Diseases of the National Health Commission of the PR ChinaShanghai National Center for Translational MedicineShanghai 200025China CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyCenter for Influenza Research and Early-warning(CASCIRE)CAS-TWAS Center of Excellence for Emerging Infectious Diseases(CEEID)Chinese Academy of SciencesBeijing 100101China Laboratory Animal Center Chinese Center for Disease Control and Prevention(China CDC)Beijing 102206China Department of Respiratory and Critical Care Medicine Institute of Respiratory DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Cardiology Institute of Cardiovascular DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China School of Laboratory Medicine and Life Sciences Wenzhou Medical UniversityWenzhouZhejiang 325000China University of Chinese Academy of Sciences Beijing 100049China

Severe acute respiratory syndrome coronavirus 2(SARSCo V-2)infection can result in more severe syndromes and poorer outcomes in patients with diabetes and ***,the precise mechanisms responsible for the combined impact of coronavirus disease 2019(COVID-19)and diabetes have not yet been elucidated,and effective treatment options for SARS-Co V-2-infected diabetic patients remain *** investigate the disease pathogenesis,K18-h ACE2 transgenic(h ACE2^(Tg))mice with a leptin receptor deficiency(h ACE2-Lepr^(-/-))and high-fat diet(h ACE2-HFD)background were *** two mouse models were intranasally infected with a 5×10^(5) median tissue culture infectious dose(TCID_(50))of SARSCo V-2,with serum and lung tissue samples collected at 3days *** h ACE2-Lepr^(-/-)mice were then administered a combination of low-molecular-weight heparin(LMWH)(1 mg/kg or 5 mg/kg)and insulin via subcutaneous injection prior to intranasal infection with1×10^(4) TCID_(50)of SARS-Co *** drug administration continued until the euthanasia of the *** of viral RNA loads,histopathological changes in lung tissue,and inflammation factors were *** demonstrated similar SARS-Co V-2 susceptibility in h ACE2^(Tg)mice under both lean(chow diet)and obese(HFD)***,compared to the h ACE2-Lepr^(+/+)mice,h ACE2-Lepr^(-/-)mice exhibited more severe lung injury,enhanced expression of inflammatory cytokines and hypoxia-inducible factor-1α(HIF-1α),and increased ***,combined LMWH and insulin treatment effectively reduced disease progression and severity,attenuated lung pathological changes,and mitigated inflammatory *** conclusion,preexisting diabetes can lead to more severe lung damage upon SARS-Co V-2 infection,and LMWH may be a valuable therapeutic approach for managing COVID-19patients with diabetes.

关键词： SARS-CoV-2 Diabetes Mouse model Heparin Antiviral therapy

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仿生扑翼无人系统研究综述

智能系统学报 2023年第3期18卷 410-439页

作者：王军张震李富强卢宣成中国矿业大学信息与控制工程学院江苏徐州221000

仿生扑翼无人系统是模仿自然界中蝙蝠、鸟类和昆虫的飞行方式研发的一种新型无人系统,可在高度复杂和动态的环境中飞行,并对环境的干扰具有良好的鲁棒性,是机器人领域未来发展的重要研究方向之一。本文对近年来国内外扑翼无人系统方面... 详细信息

仿生扑翼无人系统是模仿自然界中蝙蝠、鸟类和昆虫的飞行方式研发的一种新型无人系统,可在高度复杂和动态的环境中飞行,并对环境的干扰具有良好的鲁棒性,是机器人领域未来发展的重要研究方向之一。本文对近年来国内外扑翼无人系统方面的相关研究进行综述,分别从空气动力学、系统识别与建模、感知和控制等几个方面介绍了扑翼无人系统的最新进展,最后提炼出扑翼无人系统的各个方面的挑战,并对该领域未来的发展和研究方向做出了展望。

关键词：扑翼无人系统空气动力学数值模拟系统识别建模系统感知姿态控制振动控制

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Light touch in quantum:an interview with *** Haroche

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Advanced Photonics 2024年第1期6卷 10-16页

作者： Chao-Yang lu CAS Centre for Excellence in Quantum Information and Quantum Physics HefeiChina NYU Shanghai NYU-ECNU Institute of PhysicsShanghaiChina University of Science and Technology of China Department of Modern PhysicsShanghaiChina

lu:Hello,***,nice to see you again!Let us start the *** 2012,you and David Wineland won the Nobel Prize in *** you describe your work in simple terms to a general audience?And can you tell us how you were originally interested in this field and got into this study?Haroche:Maybe I should start with the second part of the question.I got interested in atomic physics when I was a student atÉcole Normale Supérieure(ENS)in Paris in the 1960s.

关键词： quantum interview Serge