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Development of Marker-Free Transgenic Cry1Ab Rice with Lepidopteran Pest Resistance by Agrobacterium Mixture-Mediated Co-transformation

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Rice science 2009年第3期16卷 181-186页

作者： QI Yong-bin YE Sheng-hai lu yan-ting JIN Qing-sheng ZHANG Xiao-ming Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Biotechnology Institute Zhejiang University Hangzhou 310029 China

CrylAb gene was transformed into four rice varieties, Zhejing 22, Zhejing 27, Jiahua 1 and Xiushui 63 mediated by Agrobacterium-mixture co-transformation. Rice genotype had an important effect on callus induction and transformation efficiency. Different mixtures of Agrobacterium strains （EHA105 and EHA101） contained Hpt and CrylAb genes resulted in different frequencies of resistant calli. There was no correlation between the frequency of transformants with the ratio of the Agrobacterium strain mixture contained Hpt and CrylAb genes. A total of 509 transgenic plants were obtained from the four rice varieties, and 272 T2 progenies were analyzed for CrylAb and Hpt genes. PCR analysis revealed that 412 regenerated plants were Hpt positive （80.94%）, 62 plants were also CrylAb co-transformants （15.05% in total frequency）, and 42 plants among the 272 T2 progenies were CrylAb positive but Hpt negative. This suggests that marker-free transgenic plants could be produced by co-transformation mediated by mixed Agrobacterium strains with the selectable marker gene and target gene Southern blot analysis of five independent marker-free T2 transgenic lines co-transformed from Zhejing 22 showed that CrylAb gene had been inserted into rice genome with a single copy. The transgenic plants showed significantly stronger resistance to lepidopteron than the non-transgenic plants under no application of insecticides against lepidopteron.

关键词： rice (Oryza sativa) Agrobacterium mixture-mediated co-transformation CrylAb gene Hpt gene gene transfer marker-free transgenic plants pest resistance

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碳纤维布与钢板复合加固钢筋混凝土梁刚度分析

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铁道学报 2007年第1期29卷 72-76页

作者：卢亦焱周婷武汉大学土木建筑工程学院湖北武汉430072

碳纤维布与钢板复合加固是一种新型的加固方法,可应用于桥梁等大跨度梁的加固。本文对12根碳纤维布与钢板复合加固钢筋混凝土梁进行了试验研究,结果表明,碳纤维布与钢板复合加固方法可较大幅度地提高钢筋混凝土梁的截面刚度,减小其变形... 详细信息

碳纤维布与钢板复合加固是一种新型的加固方法,可应用于桥梁等大跨度梁的加固。本文对12根碳纤维布与钢板复合加固钢筋混凝土梁进行了试验研究,结果表明,碳纤维布与钢板复合加固方法可较大幅度地提高钢筋混凝土梁的截面刚度,减小其变形。在试验研究的基础上,分析钢筋混凝土梁在碳纤维布与钢板复合加固后截面刚度的变化规律,并运用刚度解析法对截面刚度进行了计算,同时结合相关试验资料的统计分析,提出复合加固梁截面刚度的简化计算公式。其计算结果与试验结果吻合较好,可为实际工程应用提供参考。

关键词：碳纤维布钢板复合加固钢筋混凝土梁刚度

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AutoCAD Express Tools在采煤作业循环图表制作中的应用

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煤炭技术 2010年第9期29卷 78-78,92页

作者：路延廷黑龙江省国土资源厅哈尔滨150027

根据采煤作业循环图表中线型的特点,结合AutoCAD Express Tools Volumes效率工具库的具体操作过程,介绍了采煤作业循环图表中多种线型的制作方法。着重分析了形定义文件的概念和形文件的创建方法,以及在使用过程中存在的问题和解决方法。

关键词：循环图表线型形定义文件创建

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中医治疗糖尿病抑郁症共病的研究进展

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中国实验方剂学杂志 2022年第2期28卷 266-272页

作者：潘瑾薛晓燕马婷逯艳婷山东中医药大学济南250355 山东中医药大学山东省中医经典名方协同创新中心济南250355

糖尿病和抑郁症均为难治愈慢性疾病,二者相生相伴,临床上称之为糖尿病抑郁症共病。大量的流行病学和临床研究已证实,糖尿病与抑郁症交互,导致糖尿病抑郁症共病病情复杂,治疗难度大,预后差。现有临床治疗糖尿病抑郁症共病的方法大多将糖... 详细信息

糖尿病和抑郁症均为难治愈慢性疾病,二者相生相伴,临床上称之为糖尿病抑郁症共病。大量的流行病学和临床研究已证实,糖尿病与抑郁症交互,导致糖尿病抑郁症共病病情复杂,治疗难度大,预后差。现有临床治疗糖尿病抑郁症共病的方法大多将糖尿病与抑郁症作为两个独立的疾病进行治疗,存在显效率低不良反应强等诸多缺陷。中医治疗糖尿病抑郁症共病具有多靶点、多途径、整体协调的特点,能够调动机体多个系统之间的协同作用,在糖尿病抑郁症共病的治疗中显现出诸多优势。但是中医治疗糖尿病抑郁症共病的疗效评价大多只局限于临床症状的改善、血糖水平的控制及抑郁量表评分,缺少作用机制的深入研究,因此,许多研究者致力于探索中医药治疗糖尿病合并抑郁症的潜在作用机制。明确糖尿病抑郁症的共病机制是治疗糖尿病抑郁症共病的关键枢纽,在此,笔者回顾了中医治疗糖尿病抑郁症共病的相关文献,根据其作用机制进行介绍,并概括整理了糖尿病抑郁症的共病机制,主要包括胰岛素抵抗机制、氧化应激和炎症机制、神经系统紊乱机制。各个机制之间相互作用,进一步加重了糖尿病抑郁症共病的复杂性,笔者绘制了糖尿病与抑郁症共病的机制通路图,以期为临床诊疗提供新思路。

关键词：糖尿病抑郁症共病机制中药复方中医外治

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Al_2O_3/SiC纳米复相陶瓷材料的研究进展

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材料科学与工艺 2005年第4期13卷 337-340页

作者：颜鲁婷司文捷苗赫濯北京交通大学理学院北京100044 清华大学材料科学与工程系北京100084

Al2O3/SiC纳米复相陶瓷由于具有优异的室温及高温机械性能而成为结构陶瓷领域研究的热点.本文就Al2O3/SiC纳米复相陶瓷的不同制备加工方式及增强增韧机理进行了详细的阐述.其中粉体的均匀混合是制备过程的关键因素,残余应力及裂纹偏转... 详细信息

Al2O3/SiC纳米复相陶瓷由于具有优异的室温及高温机械性能而成为结构陶瓷领域研究的热点.本文就Al2O3/SiC纳米复相陶瓷的不同制备加工方式及增强增韧机理进行了详细的阐述.其中粉体的均匀混合是制备过程的关键因素,残余应力及裂纹偏转导致的穿晶断裂以及裂纹尖端SiC颗粒的桥联作用是复相陶瓷强度和韧性增加的主导因素.

关键词：纳米复相陶瓷制备显微结构机理

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Relation between Starlight and Nebular Emission Lines of Star-Forming Galaxies

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Research in Astronomy and Astrophysics 2005年第3期5卷 221-228页

作者： Hong-Lin lu, Hong-yan Zhou, ting-Gui Wang, Zhen-Quan Zhuang, Xiao-Bo Dong, Jun-Xian Wang and Cheng Li Department for Electronics of Science & technology, University of Science and Technology of China, Hefei 230026 Center for Astrophysics, University of Science and Technology of China, Hefei 230026 Department for Electronics of Science & technology University of Science and Technology of China Hefei 230026 Center for Astrophysics University of Science and Technology of China Hefei 230026

We present an exercise that intends to establish a relationship between the strength of nebular emission lines and optical stellar features in the spectrum of a galaxy. After accurately subtracting the stellar continuum and the underlying stellar absorption, we made reliable measurements of the emission lines of all the galaxies in the Sloan Digital Sky Survey Data Release 2 (SDSS DR2). More than 4000 star-forming galaxies with high S/N ratio of both the stellar spectrum and the emission lines are selected. These galaxy spectra are fitted with the 10 PCs of Yip et al., after all the emission line regions have been filtered out. We find that the flux of hydrogen Balmer emission lines, Ha and Hβcan be well recovered from the PCs, while the metal lines are not well reproduced. The fluxes of Ha and Hβmeasured from the PC-reconstructed spectra and from the observed spectra agree well with an rms scatter of only - 0.1 dex. This result suggests that, with moderate spectral resolution and S/N ratio, the optica

关键词： galaxies: fundamental parameters - galaxies: starburst - radiation: lines continuum

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