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Functionalized Hydrogels for Articular cartilage Tissue Engineering

Engineering 2022年第6期8卷 71-90页

作者： Liangbin Zhou Peng Guo Matteo d’Este Wenxue Tong Jiankun Xu Hao Yao Martin J.Stoddart Gerjo J.v.M.van Osch Kevin Ki-Wai ho Zhen Li Ling Qin Musculoskeletal Research Laboratory of department of Orthopaedics&Traumatology Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Innovative Orthopaedic Biomaterials and drug Translational Research Laboratory Li Ka Shing Institute of Health SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China AO Research Institute davos DavosCH 7270Switzerland Innovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury Department of Orthopaedic SurgeryThe Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen 518000China department of Orthopaedics and Sports Medicine&department of Otorhinolaryngology Erasmus MCUniversity Medical CenterRotterdam 3000 CAthe Netherlands f Department of Biomechanical EngineeringDelft University of TechnologyDelft 2600 AAthe Netherlands Centre for Translational Medicine Research and development Shenzhen Institute of Advanced TechnologyThe Chinese Academy of SciencesShenzhen 518000China

Articular cartilage(AC)is an avascular and flexible connective tissue located on the bone surface in the diarthrodial *** defects are common in the knees of young and physically active *** of the lack of suitable tissue-engineered artificial matrices,current therapies for AC defects,espe-cially full-thickness AC defects and osteochondral interfaces,fail to replace or regenerate damaged carti-lage *** rapid research and development advancements in AC tissue engineering(ACTE),functionalized hydrogels have emerged as promising cartilage matrix substitutes because of their favor-able biomechanical properties,water content,swelling ability,cytocompatibility,biodegradability,and lubricating *** can be rationally designed and conveniently tuned to simulate the extracel-lular matrix of *** article briefly introduces the composition,structure,and function of AC and its defects,followed by a comprehensive review of the exquisite(bio)design and(bio)fabrication of func-tionalized hydrogels for AC ***,we summarize the challenges encountered in functionalized hydrogel-based strategies for ACTE both in vivo and in vitro and the future directions for clinical translation.

关键词： Articular cartilage Functionalized hydrogels cartilage repair cartilage tissue engineering Clinical translation

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luminescent cyclometalated iridium(Ⅲ) dipyridoquinoxaline indole complexes as biological probes

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Science China Chemistry 2010年第10期53卷 2091-2098页

作者： LO Kenneth Kam-Wing LEUNG Arthur ho-hon department of Biology and Chemistry City University of Hong Kong

Four luminescent cyclometalated iridium(Ⅲ) dipyridoquinoxaline complexes appended with an indole moiety [Ir(N^C)2(N^N)] (PF6) (HN^C = 2-phenylpyridine, Hppy; N^N = 2-(N-(2-(indole-3-acetamido)ethyl)aminocarbonyl)dipyrido[3,2-f:2′,3′-h]quinox- aline, dpqC2indole (1a), N^N = 2-(N-(6-(indole-3-acetamido)hexyl)aminocarbonyl)dipyrido[3,2-f:2′,3′-h]quinoxaline, dpqC6indole (1b); HN^C = 7,8-benzoquinoline, Hbzq, N^N = dpqC2indole (2a), N^N = dpqC6indole (2b)) have been synthesized and characterized. Upon irradiation, all the complexes displayed moderately intense and long-lived luminescence under ambient condi-tions and in 77 K glass. On the basis of the photophysical data, the emission of the complexes has been assigned to an excited state of triplet metal-to-ligand charge-transfer (3MLCT) ((dπ(Ir)→π*(N^N)) character. Cyclic voltammetric studies revealed indole-based and iridium-based oxidations at ca. +1.10 v and +1.24 v vs. SCE, respectively, and ligand-based reductions at ca. -1.07 to-2.29 v vs. SCE. The interactions of the complexes with an indole-binding protein, bovine serum albumin (Bsa), have been examined by emission titrations.

关键词： binding indole iridium luminescence probes

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Spontaneous ca2+ oscillations in subcellular compartments of vascular smooth muscle cells rely on different ca2+ pools

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Cell Research 2004年第5期14卷 379-388页

作者： Olesya d. FEdORYAK,Yvonne SEARLS,Irina v. SMIRNOva,douglas M. BURNS,Lisa STEHNO-BITTEL departments of Physical Therapy and Rehabilitation Sciences University of Kansas Medical Center Kansas City KS 66160 Usa departments of Physical Therapy and Rehabilitation Sciences University of Kansas Medical Center Kansas City KS 66160 Usa department of Anatomy and Cell Biology University of Kansas Medical Center Kansas City KS 66160 Usa departments of Physical Therapy and Rehabilitation Sciences University of Kansas Medical Center Kansas City KS 66160 Usa va Medical Center Kansas City MO 64128 Usa departments of Physical Therapy and Rehabilitation Sciences University of Kansas Medical Center Kansas City KS 66160 Usa department of Anatomy and Cell Biology University of Kansas Medical Center Kansas City KS 66160 Usa

Spontaneous ca2+ oscillations in vascular smooth muscle cells have been modeled using a single ca2+ pool. This report describes spontaneous ca2+ oscillations dependent on two separate ca2+ sources for the nuclear versus cytoplas- mic compartments. Changes in free intracellular ca2+ were monitored with ratiometric ca2+- fluorophores using confo- cal microscopy. On average, spontaneous oscillations developed in 79% of rat aortic smooth muscle cells that were synchronous between the cytoplasm and nucleus. Reduction of extracellular ca2+ (decreased the frequency and amplitude of the cytoplasmic oscillations with 48% of the oscillations asynchronous between the nuclear and cytoplasmic compartments. Similar results were obtained with the ca2+ channel blockers, nimodipine and diltiazem. Arg-vasopressin (AvP) induced a rapid release of intracellular ca2+ stores that was greater in the nuclear compartment (4.20 ± 0.23 ratio units, n = 56) than cytoplasm (2.54 ± 0.28) in cells that had spontaneously developed prior oscillations. Conversely, cells in the same conditions lacking oscillations had a greater AvP-induced ca2+ transient in the cytoplasm (4.99 ± 0.66, n = 17) than in the nucleus (2.67 ± 0.29). Pre-treatment with ca2+ channel blockers depressed the AvP responses in both compartments with the cytoplasmic ca2+ most diminished. depletion of internal ca2+ stores prior to AvP exposure blunted the nuclear response, mimicking the response of cells that lacked prior oscillations. Spontaneous oscillating cells had a greater sarcoplasmic reticulum network than cells that did not oscillate. We propose that sponta- neous nuclear oscillations rely on perinuclear sarcoplasmic reticulum stores, while the cytoplasmic oscillations rely on ca2+ influx.

关键词： oscillations vascular smooth muscle cells nuclear ca2+ vasopressin thapsigargin diltiazem nimodipine.

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Novel Fluorene-thiophene Copolymers and Polymer Blends for Photonic devices

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复旦学报（自然科学版） 2007年第5期46卷 623-页

作者： v.CIMROva P.PvLACKva d.vYPRACHTICKY I.KMINEK J.TOUSEK Institute of Macromolecular Chemistry Academy of Sciences of the Czech RepublicHeyrovského nám. 2CZ-162 06 Praha 6 Czech Republic department of Macromolecular Physics Faculty of Mathematics and PhysicsCharles University V Hole ovikách 2 18000 Prague 8 Czech Republic

1 Results In this contribution we deal with photophysical and photoelectrical study of novel fluorene-thiophene copolymers, and thiophene-based oligomers and polymers, the syntheses of which are reported in our other contributions. Information about electronic structure of the polymers was obtained from cyclic voltammetry and absorption spectroscopy. The absorption of the polymers under study covers the whole visible region; the low band-gap polymer (Eg=1.35 ev) exhibited the long-wavelength absorption ...

关键词： conjugated polymers photophysics electronic structure photovoltaics

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Prediction of van hove singularity systems in ternary borides

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npj Computational Materials 2023年第1期9卷 247-257页

作者： Yang Sun Zhen Zhang Andrew P.Porter Kirill Kovnir Kai-Ming ho vladimir Antropov department of Physics Xiamen University361005 XiamenChina department of Physics and Astronomy Iowa State UniversityAmesIA 50011USA department of Chemistry Iowa State UniversityAmesIA 50011USA Ames National Laboratory U.S.Department of EnergyAmesIA 50011USA

A computational search for stable structures among bothαandβphases of ternary ATB4 borides(A=Mg,ca,Sr,Ba,Al,Ga,and Zn,T is 3d or 4d transition elements)has been *** found thatα-ATB4 compounds with A=Mg,ca,Al,and T=v,Cr,Mn,Fe,Ni,and Co form a family of structurally stable or almost stable *** systems are metallic in non-magnetic states and characterized by the formation of the localized molecular-like state of 3d transition metal atom dimers,which leads to the appearance of numerous van hove singularities in the electronic *** closeness of such singularities to the Fermi level can be easily tuned by electron *** the atoms in the middle of the 3d row(Cr,Mn,and Fe),these singularities led to magnetic instabilities and magnetic ground states with a weakly metallic or semiconducting *** states appear as non-trivial coexistence of the different spin ladders formed by magnetic dimers of 3d *** magnetic states can be characterized as an analog of the spin glass *** attempts to produce MgFeB4 and associated challenges are discussed,and promising directions for further synthetic studies are formulated.

关键词： metallic ternary magnetic

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Epistaxis in end stage liver disease masquerading as severe upper gastrointestinal hemorrhage

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World Journal of Gastroenterology 2014年第38期20卷 13993-13998页

作者： Marine camus dennis M Jensen Jason d Matthews Gordon v Ohning Thomas O Kovacs Rome Jutabha Kevin A Ghassemi Gustavo A Machicado Gareth S dulai CURE Hemostasis Research Group CURE Digestive Diseases Research CenterDavid Geffen School of Medicine at University of California division of digestive diseases at University of california Los AngelesRonald Reagan Medical Center department of Gastroenterology Lariboisiere Hospital CURE Hemostasis Research Group CURE Digestive Diseases Research CenterDavid Geffen School of Medicine at University of CaliforniaLos AngelesLos AngelesCA 90095United States Gastroenterology division at va Greater Los Angeles Healthcare System Hunterdon Gastrointestinal Associates

AIM: To describe the prevalence, diagnosis, treatment, and outcomes of end stage liver disease (ESLd) patients with severe epistaxis thought to be severe upper gastrointestinal hemorrhage (UGIH).

关键词： Epistaxis Upper gastrointestinal bleeding End stage liver disease Cirrhosis Nasogastric tube Liver transplantation digestive bleeding Nasal packing Coagulopathy

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Laser Machined Fiber‑Based Microprobe: Application in Microscale Electroporation

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Advanced Fiber Materials 2022年第4期4卷 859-872页

作者： Jongwoon Kim Yajun Zhao Shuo Yang Ziang Feng Anbo Wang Rafael v.davalos Xiaoting Jia Bradley department of Electrical and Computer Engineering Virginia TechBlacksburgVA 24060USA department of Biomedical Engineering and Mechanics Virginia TechBlacksburgVA 24061USA

Microscale electroporation devices are mostly restricted to in vitro experiments(i.e.,microchannel and microcapillary).Novel fiber-based microprobes enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to *** developed a flexible,fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining *** fiber consists of four copper electrodes(80μm),one microfluidic channel(30μm),and has an overall diameter of 400μ*** dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser *** feasibility of the fiber probe was validated through numerical simulations and in vitro *** reversible and irreversible microscale electroporation was observed in a 3d collagen scaffold(seeded with U251 human glioma cells)using fluorescent *** ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical *** computational and experimental results of the working fiber-based microprobe suggest the feasibility of in vivo microscale electroporation in space-sensitive areas,such as the deep brain.

关键词： Fiber probe Polymer Micromachine Irreversible electroporation Reversible electroporation Microscale electroporation

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Survival after inflammatory bowel disease-associated colorectal cancer in the Colon cancer Family Registry

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World Journal of Gastroenterology 2013年第21期19卷 3241-3248页

作者： Scott v Adams dennis J Ahnen John A Baron Peter T campbell Steven Gallinger William M Grady Loic LeMarchand Noralane M Lindor John d Potter Polly A Newcomb cancer Prevention Public Health SciencesFred Hutchinson Cancer Research CenterSeattleWA 98109United States department of Medicine Denver VA Medical Center and University of Colorado School of MedicineDenverCO 80045United States department of Medicine University of North CarolinaChapel HillNC 27599United States Epidemiology Research Program Department of Intramural ResearchAmerican Cancer SocietyAtlantaGA 30303United States division of General Surgery University of TorontoTorontoON M5G2C4Canada cancer Research Center of Hawaii University of HawaiiHonoluluHI 96813United States department of Health Science Research Mayo Clinic ArizonaScottsdaleAZ 85259United States

AIM: To investigate the survival of individuals with colorectal cancer (CRC) with inflammatory bowel disease (IBd-associated CRC) compared to that of individuals without IBd diagnosed with CRC. METhodS: Epidemiologic, clinical, and follow-up data were obtained from the Colon cancer Family Registry (Colon CFR). IBd-associated cases were identified from self-report of physician diagnosis. For a subset of participants, medical records were examined to confirm self-report of IBd. Cox proportional hazards regression was applied to estimate adjusted hazard ratios (aHR) and 95%CI of mortality, comparing IBd-associated to non-IBd-associated CRC, adjusted for age at CRC diagnosis, sex, Colon CFR phase, and number of prior endoscopies. Following imputation to complete CRC stage information, adjustment for CRC stage was examined. RESULTS: A total of 7202 CRC cases, including 250 cases of IBd-associated CRC, were analyzed. Over a twelve year follow-up period following CRC diagnosis, 2013 and 74 deaths occurred among non-IBd associated CRC and IBd-associated CRC patients, respectively. The difference in survival between IBd-associated and non-IBd CRC cases was not statistically significant (aHR = 1.08; 95%CI: 0.85-1.36). however, the assumption of proportional hazards necessary for valid inference from Cox regression was not met over the entire follow-up period, and we therefore limited analyses to within five years after CRC diagnosis when the assumption of proportional hazards was met. Over this period, there was evidence of worse prognosis for IBd-associated CRC (aHR = 1.36; 95%CI: 1.05-1.76). Results were similar when adjusted for CRC stage, or restricted to IBd confirmed in medical records. CONCluSION: These results support the hypothesis that IBd-associated CRC has a worse prognosis than non-IBd-associated CRC.

关键词： Colorectal cancer Inflammatory bowel disease Outcomes research cancer survival Inflammation

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REvIEW OF PARTICLE PHYSICS

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Chinese Physics C 2014年第9期38卷 1-4页

作者： K.A.Olive K.Agashe C.Amsler M.Antonelli J.-F.Arguin d.M.Asner H.Baer H.R.Band R.M.Barnett T.Basaglia C.W.Bauer J.J.Beatty v.I.Belousov J.Beringer G.Bernardi S.Bethke H.Bichsel O.Biebe E.Blucher S.Blusk G.Brooijmans O.Buchmueller v.Burkert M.A.Bychkov R.N.cahn M.carena A.Ceccucci A.Cerr d.Chakraborty M.-C.Chen R.S.Chivukula K.Copic G.Cowan O.dahl G.d'Ambrosio T.damour d.de Florian A.de Gouvea T.deGrand P.de Jong G.dissertor B.A.dobrescu M.doser M.drees H.K.dreiner d.A.Edwards S.Eidelman J.Erler v.v.Ezhela W.Fetscher B.d.Fields B.Foster A.Freitas T.K.Gaisser H.Gallagher L.Garren H.-J.Gerber G.Gerbier T.Gershon T.Gherghetta S.Golwala M.Goodman C.Grab A.v.Gritsan C.Grojean d.E.Groom M.Grnewald A.Gurtu T.Gutsche H.E.Haber K.Hagiwara C.Hanhart S.Hashimoto Y.Hayato K.G.Hayes M.Heffner B.Heltsley J.J.Hernandez-Rey K.Hikasa A.hocker J.holder A.holtkamp J.Huston J.d.Jackson K.F.Johnson T.Junk M.Kado d.Karlen U.F.Katz S.R.Klein E.Klempt R.v.Kowalewski F.Krauss M.Kreps B.Krusche Yu.v.Kuyanov Y.Kwon O.Lahav J.Laiho P.Langacker A.Liddle Z.Ligeti C.-J.Lin T.M.Liss L.Littenberg K.S.lugovsky S.B.lugovsky F.Maltoni T.Mannel A.v.Manohar W.J.Marciano A.d.Martin A.Masoni J.Matthews d.Milstead P.Molaro K.Monig F.Moortgat M.J.Mortonson H.Murayama K.Nakamura M.Narain P.Nason S.Navas M.Neubert P.Nevski Y.Nir L.Pape J.Parsons C.Patrignani J.A.Peacock M.Pennington S.T.Petcov Kavli IPMU A.Piepke A.Pomarol A.Quadt S.Raby J.Rademacker G.Raffel B.N.Ratcliff P.Richardson A.Ringwald S.Roesler S.Rolli A.Romaniouk L.J.Rosenberg J L.Rosner G.Rybka C.T.sachrajda Y.sakai G.P.salam S.sarkar F.sauli O.Schneider K.Scholberg d.Scott v.Sharma S.R.Sharpe M.Silari T.Sjostrand P.Skands J.G.Smith G.F.Smoot S.Spanier H.Spieler C.Spiering A.Stahl T.Stanev S.L.Stone T.Sumiyoshi M.J.Syphers F.Takahashi M.Tanabashi J.Terning L.Tiator M.Titov N.P.Tkachenko N.A.Tornqvist d.Tovey G.valencia G.venanzoni M.G.vincter P.vogel A.vogt S.P.Wakely W.Walkowiak C.W.Walter d.R.Ward G.Weiglein d.H.Weinberg E.J.Weinberg M.White L.R.Wiencke C.G.Wohl L.Wolfenstein J.Womersley C.L. University of Minnesota School of Physics and Astronomy116 Church St.S.E.MinneapolisMN 55455USA University of Maryland Department of PhysicsCollege ParkMD 20742-4111USA Albert Einstein Center for Fundamental Physics Universitat BernCH-3012 BernSwitzerland Lab.Nazionali di Frascati dell'INFN CP 13via E.Fermi40I-00044 Frascati(Roma)Italy departement de physique Universite de MontrealC.P.6128succ.centre-villeMontreal(Quebec) H3C 3J7Canada Pacific Northwest National Laboratory 902 Battelle BoulevardRichlandWA 99352USA department of Physics and Astronomy University of OklahomaNormanOK 73019USA department of Physics University of WisconsinMadisonWI 53706USA Physics division Lawrence Berkeley National Laboratory1 Cyclotron RoadBerkeleyCA 94720USA CERN European Organization for Nuclear ResearchCH-1211 Geneve 23Switzerland department of Physics The Ohio State University191 W.Woodruff Ave.ColumbusOH 43210 COMPAS Group Institute for High Energy PhysicsRU- 142284ProtvinoRussia LPNHE IN2P3-CNRS et Universites de Paris 6 et 7F-75252 ParisFrance Max-Planck-Institute of Physics 80805 MunichGermany department of Physics University of WashingtonSeattleWA 98195USA ludwig-Maximilians-Universitat Fakultdt für PhysikSchellingstr.4D-80799 MünchenGermany Enrico Fermi Institute and department of Physics University of ChicagoChicagoIL 60637-1433USA department of Physics Syracuse UniversitySyracuseNY13244USA department of Physics Columbia University538 W.120th StreetNew YorkNY10027 USA High Energy Physics Group Blackett LaboratoryImperial CollegePrince Consort RoadLondon SW7 2AZUK Jefferson Lab 12000 Jefferson Ave.Newport NewsVA 23606USA department of Physics University of VirginiaPO Box 400714CharlottesvilleVA 22904USA Fermi National Accelerator Laboratory P.O.Box 500BataviaIL 60510USA Kavli Institute for Cosmological Physics University of ChicagoChicagoIL 60637-1433USA department of Physics and Astronomy University of SussexFalmerBrighton BN1 9QHUK department o

The Review summarizes much of particle physics and cosmology. Using data from previous editions, plus 3,283 new measurements from 899 papers, we list, evaluate, and average measured properties of gauge bosons and the recently discovered Higgs boson, leptons, quarks, mesons, and baryons. We summarize searches for hypothetical particles such as heavy neutrinos, supersymmetric and technicolor particles, axions, dark photons, etc. All the particle properties and search limits are listed in Summary Tables. We also give numerous tables, figures, formulae, and reviews of topics such as Supersymmetry, Extra dimensions, Particle detectors, Probability, and Statistics. Among the 112 reviews are many that are new or heavily revised including those on： dark Energy, Higgs Boson Physics, Electroweak Model, Neutrino Cross Section Measurements, Monte carlo Neutrino Generators, Top Quark, dark Matter, dynamical Electroweak Symmetry Breaking, Accelerator Physics of Colliders, High-Energy Collider Parameters, Big Bang Nucleosynthesis, Astrophysical Constants and Cosmological Parameters. A booklet is available containing the Summary Tables and abbreviated versions of some of the other sections of this full Review. All tables, listings, and reviews （and errata） are also available on the Particle data Group website： http ：//pdg. lbl. gov.

关键词： REvIEW OF PARTICLE PHYSICS

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Characterization of Ablation Thresholds for 3d-Cultured Patient-derived Glioma Stem Cells in Response to High-Frequency Irreversible Electroporation

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Research 2019年第1期2019卷 1005-1018页

作者： J.W.Ivey E.M.Wasson N.Alinezhadbalalami A.Kanitkar W.debinski Z.Sheng R.v.davalos S.S.verbridge School of Biomedical Engineering and Sciences Virginia Tech-Wake Forest UniversityBlacksburgVA 24061USA department of Mechanical Engineering Virginia TechBlacksburgVA 24061USA Brain Tumor Center of Excellence Comprehensive Cancer CenterWake Forest Baptist Medical CenterWinston-SalemNC 27157USA virginia Tech carilion Research Institute RoanokeVA 24061USA department of Internal Medicine Virginia Tech Carilion School of MedicineRoanokeVA 24016USA Faculty of Health Science Virginia TechBlacksburgVA 24061USA

High-frequency irreversible electroporation(H-FIRE)is a technique that uses pulsed electric felds that have been shown to ablate malignant *** order to evaluate the clinical potential of H-FIRE to treat glioblastoma(GBM),a primary brain tumor,we have studied the efects of high-frequency waveforms on therapy-resistant glioma stem-like cell(GSC)*** demonstrate that patient-derived GSCs are more susceptible to H-FIRE damage than primary normal *** selectivity presents an opportunity for a degree of malignant cell targeting as bulk tumor cells and tumor stem cells are seen to exhibit similar lethal electric feld thresholds,signifcantly lower than that of healthy ***,neural stem cell(NSC)populations also exhibit a similar sensitivity to these *** observation may suggest that diferent considerations be taken when applying these therapies in younger versus older patients,where the importance of preserving NSC populations may impose diferent restrictions on *** also demonstrate variability in threshold among the three patient-derived GSC lines studied,suggesting the need for personalized cell-specifc characterization in the development of potential clinical *** work may provide further useful insights regarding this patient-dependent variability observed that could inform targeted and personalized treatment.

关键词： clinical younger irreversible

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