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Air Temperature and Emersion Time Can Affect the Survival Rate and Ammonium Loading of Swimming Crab Portunus trituberculatus Exposed to Air

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Journal of Ocean University of China 2020年第3期19卷 643-652页

作者： lu yunliang ZHU Boshan ZHANG Dan LI Yuquan Marine Science and Engineering College Qingdao Agricultural UniversityQingdao 266109China Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Function Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266235China Tianjin Key Laboratory of Aqua-Ecology and Aquaculture Fisheries CollegeTianjin Agricultural UniversityTianjin 300384China

Ammonium overloading is a common response of aquatic organisms to air exposure during transport. This study elucidated the relationship between ammonium overloading and mortality of crab Portunus trituberculatus. Additionally, we also explored the effects of emersion time and air temperature on ammonium loading and concomitant physiological change. To test the air temperature effect, the crab was exposed to 16, 23 and 30℃ in air for 3 h, respectively, and then recovered in seawater at 23℃ for 12 h. To test the emersion time effect, crab was exposed to 23℃ in air for 0.5 and 3 h, respectively, and then recovered in seawater at 23℃ for 12 h. In the control group, crab was always immersed at 23℃. At each time interval(0.5, 1.5 and 3 h during air exposure and 0.5, 2, 4 and 12 h during recovery), ammonium excretion rate, level of total ammonium, total free amino acids and urea concentration in hemolymph and the hepatopancreas enzyme activity involved in detoxifying ammonium were analysed. Results showed that crab mortality was positively related with emersion time and temperature while ammonium loading was lower at 16 and 30℃ than at 23℃. For crab experiencing thermal inconsistence of culture media(i.e., 16 or 30℃), they were higher in ammonium excretion rate and activities of ammonium detoxification enzymes, which may be the reason that they had a lower ammonium loading. Prolonged emersion time(3.0 h vs. 0.5 h) increased the ammonium overloading and the activity of ammonium detoxification pathways in crab. Our results demonstrated that emersion-induced ammonium overloading may not be the main reason leading to P. trituberculatus death during air exposure and subsequent recovery. When the culture medium changed, thermal variation, compared with constant temperature, could reduce ammonium overloading in crab by elevating the activities of ammonium detoxification enzymes and ammonium excretion rate during recovery period.

关键词： Portunus trituberculatus air temperature emersion time ammonium loading

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Morphological and histological changes in the brains of turbot(Scophthalmus maximus)with gonadal development

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Acta Oceanologica Sinica 2022年第12期41卷 115-122页

作者： Chunyan Zhao Liang Chi Yongshuang Xiao Bing Li yunliang lu Yanting Cui Wenqi Wang Jun Li School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China CAS Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Marine Biology and Biotechnology Laboratory Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in ***(Scophthalmus maximus)has become an economically important marine fish in Europe and North *** research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm ***,the morphology and histology of the organs in the HPG axis have not been *** this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal *** results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory *** telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal *** torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal *** actual total brain volume in mature males was significantly greater(plume in male turbot significantly increased(plustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.

关键词： turbot brain pituitary gonadal development morphology histology

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The Fire Fighters Protecting The Potala Palace

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China's Tibet 2008年第2期19卷 65-69页

作者： Jia Lijun lu yunliang

The Potala Palace is situated on the"red"mountain of Lhasa.A group of police fire fighters, unknown to most people,are actually the"guardians of the national treasures"and are living all year around inside the Potala *** they have formed a consolidated"fireproof wall"to protect this world famous cultural *** they took up residence 23 years ago, there have been no accidental fires in the palace.

关键词： The Fire Fighters Protecting The Potala Palace

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EXTRACTION OF THE STANDARD ENTHALPY OF FORMATION OF MINERALS FROM REVERSED EXPERIMENTAL DATA WITH INS

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矿物岩石 1992年第4期 1-5页

作者： Yin Huian Han Wenxi lu Cheng Wang yunliang(Chengdu College of Geology,Chengdu 610059,PRC)

An improvement on the new method of Intervalhalving-Scanning (INS) proposed by the senior author for derivation of thermodynamic properties of minerals from reversed experiment (REP) data has been made in the present work. The treatment of the REP data of 6 reactions in the system MgO-SiO-HO and the derivation of △H(?) (298. 15K) for minerals Talc,Forsterite and Anthophyllite are chosen as an example for demonstration of the application of the *** is quite different from all the methods for the derivation in the literature and throughout based on thermodynamic principles and equations so that its thermodynamical validity is thoroughgoing.

关键词： Intervalhalving-Scanning Technique Thermodynamical validity Thermodynamic properties of minerals

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Expression and Prognostic Significance of HMGCS2 in Renal Cell Carcinoma

Expression and Prognostic Significance of HMGCS2 in Renal Ce...

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2019年中国肿瘤标志物学术大会暨第十三届肿瘤标志物青年科学家论坛

作者： lu,yunliang Zhang,Zhe Zhou,Xiaoying The first affiliated hospital of guangxi medical university Life Science Institute Guangxi Medical University

Background:Kidney cancer is a malignant tumor that originates in the urinary tubular epithelial system of the renal *** incidence is ranked 6 th in men and 8 th in *** have shown that the incidence of renal cancer in China is gradually increasing.m HMGCS2 is a rate-limiting enzyme produced by ketone *** condenses acetaminophen coenzyme A and acetamidine coenzyme A to form *** step is an irreversible key step in the formation of ketone *** to reports,HMGCS2 plays an important role in the development of various ***,the clinical significance and molecular mechanism of HMGCS2 in renal cell carcinoma(RCC) remains ***,this study aimed to investigate the role of HMGCS2 in the pathogenesis of renal *** and Methods:Renal cancer tissues and adjacent tissues were collected for immunohistochemistry(IHC) analysis to observe the expression of *** HMGCS2 expression data was downloaded from the tumor genomic map(TCGA) to analyze the clinical significance of *** analysis assessed the diagnostic and prognostic significance of HMGCS2 in IHC and TCGA *** data analysis site(Aipufu) was used to analyze the association between DNA methylation and mRNA expression of HMGCS2 in renal cell ***:Analysis of IHC and TCGA data showed a significant reduction in HMGCS2 in renal cell carcinoma *** expression of HMGCS2 is associated with tumor size,metastasis and T *** analysis showed that the up-regulation of HMGCS2 predicted a decline in overall survival of ***2 is hypermethylated in promoter DNA in KIRC,and the frequency of methylation in normal tissues is ***:HMGCS2 may be a suppressor gene in the development of renal cell carcinoma,and its expression in renal cell carcinoma is *** may be related to hypermethylation of the DNA promoter region of HMGCS2 in renal cancer,and further validation is *** addition,HMGCS2 is expected

关键词： HMGCS2 renal cell carcinoma methylation immunohistochemistry diagnosis

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Vertical gradients of neutral winds observed by ICON and estimated by the Horizontal Wind Model during the geomagnetic storm on August 26−28,2021

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Earth and Planetary Physics 2025年第1期9卷 69-80页

作者： JiaWei Wu Chao Xiong YuYang Huang yunliang Zhou Department of Space Physics School of Electronic InformationHubei Luojia LaboratoryWuhan UniversityWuhan 430072China

The Michelson Interferometer for Global High-resolution Thermospheric Imaging(MIGHTI)onboard the Ionospheric Connection Explorer(ICON)satellite offers the opportunity to investigate the altitude profile of thermospheric *** this study,we used the red-line measurements of MIGHTI to compare with the results estimated by Horizontal Wind Model 14(HWM14).The data selected included both the geomagnetic quiet period(December 2019 to August 2022)and the geomagnetic storm on August 26-28,*** the geomagnetic quiet period,the estimations of neutral winds from HWM14 showed relatively good agreement with the observations from *** to the ICON observations,near the equator,zonal winds reverse from westward to eastward at around 06:00 local time(LT)at higher altitudes,and the stronger westward winds appear at later LTs at lower *** around 16:00 LT,eastward winds at 300 km reverse to westward,and vertical gradients of zonal winds similar to those at sunrise hours can be *** the middle latitudes,zonal winds reverse about 2-4 h *** winds vary more significantly than zonal winds with seasonal and latitudinal *** to the ICON observations,in the northern low latitudes,vertical reversals of meridional winds are found at 08:00-13:00 LT from 300 to 160 km and at around 18:00 LT from 300 to 200 km during the June *** reversals of meridional winds are found at 04:00-07:00 LT from 300 to 160 km and at 22:00-02:00 LT from 270 to 200 km during the December *** the southern low latitudes,meridional wind reversals occur at 08:00-11:00 LT from 200 to 160 km and at 21:00-02:00 LT from 300 to 200 km during the June *** the December solstice,reversals of the meridional wind appear at 20:00-01:00 LT below 200 km and at 06:00-11:00 LT from 300 to 160 *** the northern middle latitudes,the northward winds are dominant at 08:00-14:00 LT at 230 km during the June *** winds persist until

关键词： horizontal neutral winds vertical gradients Ionospheric Connection Explorer satellite Horizontal Wind Model 14 geomagnetic storm

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赵固二矿14040下顺槽底抽巷围岩松动圈测定

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山西焦煤科技 2022年第9期46卷 16-18,53页

作者：卢运良河南焦煤能源有限公司救护大队河南焦作454150

为确定赵固二矿14040下顺槽底抽巷围岩松动圈范围,采用CXK12(A)-Z矿用本安型钻孔成像仪对巷道围岩松动圈进行测定。测定结果表明,14040下顺槽底抽巷整体围岩松动圈深度在2.8~3.8 m,瓦斯抽采孔封孔深度应大于4 m,以防止漏气现象发生,提... 详细信息

为确定赵固二矿14040下顺槽底抽巷围岩松动圈范围,采用CXK12(A)-Z矿用本安型钻孔成像仪对巷道围岩松动圈进行测定。测定结果表明,14040下顺槽底抽巷整体围岩松动圈深度在2.8~3.8 m,瓦斯抽采孔封孔深度应大于4 m,以防止漏气现象发生,提高抽采效果。对于围岩松动圈较深、抽采钻孔封孔难度大的区段,采取壁后注浆加固等措施,改善巷道围岩条件,在提升巷道支护强度的同时能够有效保证瓦斯抽采效率。

关键词：围岩松动圈松动圈范围钻孔窥视瓦斯抽采

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干露对三疣梭子蟹抗氧化和应激能力的影响

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水产学报 2014年第12期38卷 1996-2004页

作者：姜娜王芳路允良董双林中国海洋大学水产学院海水养殖教育部重点实验室

在实验室条件下,研究了干露时间(0.5、1.5和3 h)胁迫对三疣梭子蟹抗氧化能力和应激能力的影响,初步探讨了能够较灵敏地指示梭子蟹健康状况的胁迫指标。结果显示:(1)干露时间胁迫对超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量均无显著影响... 详细信息

在实验室条件下,研究了干露时间(0.5、1.5和3 h)胁迫对三疣梭子蟹抗氧化能力和应激能力的影响,初步探讨了能够较灵敏地指示梭子蟹健康状况的胁迫指标。结果显示:(1)干露时间胁迫对超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量均无显著影响(P>0.05);但干露时间显著影响肝胰脏总抗氧化能力(T-AOC)水平、热休克蛋白70(HSP70)表达量和肌肉乳酸含量(P0.05)。1.5 h干露后MDA含量在入水恢复阶段呈先升高后降低趋势,各时间点与对照组差异不显著(P>0.05);而3 h干露后入水恢复10 h时显著增加(P<0.05)。1.5 h干露后入水恢复4 h时,T-AOC水平显著高于对照组(P<0.05);3 h干露后入水恢复0.5 h时显著升高(P<0.05),2~10 h内基本恢复到对照组水平。干露时间胁迫后,乳酸含量均在入水恢复0.5 h时达到最大值;HSP70表达量均在入水恢复2~4 h内达到峰值。研究表明,相比于其他所选指标,T-AOC水平能够较敏感地反映干露时间胁迫对机体的生理状态的影响;SOD和MDA相互配合能够较灵敏地反映不同干露时长后机体在恢复阶段的免疫水平。

关键词：三疣梭子蟹干露时间胁迫入水恢复抗氧化应激

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中国特色自贸港立法权行使路径研究

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国际商务研究 2025年第1期46卷 101-114页

作者：刘云亮卢晋海南大学海南海口570228

中国特色自贸港实施开放型经济新体制,亟需高水平开放、高质量发展的法治创新保障。自贸港立法权行使不仅需要创新自贸港法规内容体系,而且更需要创新自贸港立法路径,这充分彰显自贸港改革开放与立法创新法治保障的良性互动关系,尤其凸... 详细信息

中国特色自贸港实施开放型经济新体制,亟需高水平开放、高质量发展的法治创新保障。自贸港立法权行使不仅需要创新自贸港法规内容体系,而且更需要创新自贸港立法路径,这充分彰显自贸港改革开放与立法创新法治保障的良性互动关系,尤其凸显自贸港立法权行使路径创新与自贸港法规体系构建的新颖性。自贸港法规制定权、调法调规变通权和自贸港立法授权成为自贸港立法权行使路径创新渠道,促成自贸港立法权能拓展新内容。自贸港法规制定权需要对标国际经贸规则。自贸港调法调规聚焦适用具有更多实用性和先行性的领域。自贸港立法权行使不仅聚焦自贸港法规内容的制度型开放,立法内容致力于促进高水平开放和高质量发展,而且注重自贸港法规的合宪性与改革创新相融合的立法监督机制协同推进。敢于变通和创制立法,遵循立法授权,成为自贸港立法权行使路径规制,更是当下加快实现封关运作的立法及调法调规的需要,确保自贸港扩大制度型开放、融入国际高水平自贸网络的法治保障。

关键词：自贸港高水平开放立法创新调法调规

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赵固二矿回采巷道注浆锚索超前支护技术研究与应用

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现代矿业 2022年第11期38卷 224-227页

作者：卢运良刘栋河南焦煤能源有限公司救护大队焦作煤业(集团)新乡能源有限公司赵固二矿

巷道围岩稳定性直接关系到煤矿安全、高效生产,为保障巷道围岩稳定,以焦作煤田赵固二矿11012工作面为工程背景,综合利用现场调研、岩石力学试验、理论分析、工业性试验等手段,研究了回采巷道超前影响区域巷道顶板变形破坏特征,建立了上... 详细信息

巷道围岩稳定性直接关系到煤矿安全、高效生产,为保障巷道围岩稳定,以焦作煤田赵固二矿11012工作面为工程背景,综合利用现场调研、岩石力学试验、理论分析、工业性试验等手段,研究了回采巷道超前影响区域巷道顶板变形破坏特征,建立了上覆岩层-顶板-煤柱为统一体系的力学模型,得到了巷道围岩顶板拉伸、剪切破坏的力学判据。在上述分析的基础上,提出了适合本地质条件下的注浆锚索支护技术方案,应用后巷道围岩整体实现了高强全锚注一体化动态耦合控制。

关键词：回采巷道超前支护注浆锚索围岩稳定性

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