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Portable dielectrophoresis for biology:ADEPT facilitates cell trapping,separation,and interactions

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Microsystems & Nanoengineering 2024年第2期10卷 193-205页

作者： lourdes Albina Nirupa Julius Dora Akgül Gowri Krishnan Fabian Falk Jan Korvink Vlad Badilita Department Institute of Microstructure TechnologyKarlsruhe Institute of TechnologyHermann-von-Helmholtz-Platz 1Eggenstein-Leopoldshafen76344Baden-WürttembergGermany

Dielectrophoresis is a powerful and well-established technique that allows label-free,non-invasive manipulation of cells and particles by leveraging their electrical *** practical implementation of the associated electronics and user interface in a biology laboratory,however,requires an engineering background,thus hindering the broader adoption of the *** order to address these challenges and to bridge the gap between biologists and the engineering skills required for the implementation of DEP platforms,we report here a custom-built,compact,universal electronic platform termed ADEPT(adaptable dielectrophoresis embedded platform tool)for use with a simple microfluidic chip containing six *** versatility of the open-source platform is ensured by a custom-developed graphical user interface that permits simple reconfiguration of the control signals to address a wide-range of specific applications:(i)precision positioning of the single bacterium/cell/particle in the micrometer range;(ii)viability-based separation by achieving a 94%efficiency in separating live and dead yeast;(iii)phenotype-based separation by achieving a 96%efficiency in separating yeast and Bacillus subtilis;(iv)cell–cell interactions by steering a phagocytosis process where a granulocyte engulfs *** RGB-S ***,the set of experiments and the platform form a complete basis for a wide range of possible applications addressing various biological questions exploiting the plug-and-play design and the intuitive GUI of ADEPT.

关键词： Together separation separating

The dorsal root ganglion as a target for neurorestoration in neuropathic pain

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Neural Regeneration Research 2024年第2期19卷 296-301页

作者： Guillermo Estivill-Torrús Ana Belen Martínez-Padilla lourdes Sánchez-Salido Anne Baron-Van Evercooren Beatriz García-Díaz Instituto de Investigación Biomédica de Málaga y Plataforma en Nanomedicina(IBIMA Plataforma BIONAND) MálagaSpain Unidad Clínica de Neurociencias Hospital Regional Universitario de MálagaMálagaSpain Departamento de Biología Molecular y Bioquímica Universidad de MálagaSpain Sorbonne Université Institut du Cerveau-Paris Brain Institute-ICMINSERMCNRSAP-HPHôpital Pitié-SalpêtrièreParisFrance

Neuropathic pain is a severe and chronic condition widely found in the general *** reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in *** the processing of pain,the dorsal root ganglia constitute an important region where dorsal root ganglion neurons play a crucial role in the transmission and propagation of sensory electrical ***,the dorsal root ganglia have recently exhibited a regenerative capacity that should not be neglected in the understanding of the development and resolution of neuropathic pain and in the elucidation of innovative ***,we will review the complex interplay between cells(satellite glial cells and inflammatory cells)and factors(cytokines,neurotrophic factors and genetic factors)that takes place within the dorsal root ganglia and accounts for the generation of the aberrant excitation of primary sensory neurons occurring in neuropathic *** importantly,we will summarize an updated view of the current pharmacologic and nonpharmacologic therapies targeting the dorsal root ganglia for the treatment of neuropathic pain.

关键词： cytokines dorsal root ganglia genetic factors neuropathic pain neurotrophic factors pharmacologic and nonpharmacologic therapies satellite glial cells sensory neurons

Identification of Rhizobia Isolated from Nodules of Mexican Commercial Soybean Varieties

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American Journal of Plant Sciences 2024年第1期15卷 29-45页

作者： Cecilia Vázquez Rodríguez lourdes Vital López Jesús Gerardo García Olivares Homar Rene Gill Langarica Laboratorio de Biotecnología Vegetal Centro de Biotecnología Genómica Instituto Politécnico Nacional Reynosa México Carrera de Mantenimiento Industrial Universidad Tecnológica de Tamaulipas Norte Universidad Tecnológica Reynosa México

Rhizobia, crucial for nitrogen fixation in leguminous plants, play a vital role in soybean cultivation. This study, conducted in Mexico, a major soybean importer, aimed to identify bacteria from nodules of five soybean varieties in high-production regions. Multilocus sequence analysis (MLSA) was employed for enhanced species resolution. The study identified six Bradyrhizobium species: Bradyrhizobium japonicum USDA 110, Bradyrhizobium japonicum USDA 6, Bradyrhizobium elkanii USDA 76, Bradyrhizobium neotropicale, Bradyrhizobium lablabi, and Bradyrhizobium icense. Bradyrhizobium japonicum USDA 110 predominated in the soils, displaying symbiotic preference for the Huasteca 400 variety. However, phylogenetic analysis didn't reveal a clear association between strains, soil, and soybean variety. This research sheds light on the diversity of rhizobia in Mexican soybean cultivation, contributing to the understanding of symbiotic relationships in soybean production systems.

关键词： Nodules Soybean Housekeeping Genes MLSA Rhizobia Bradyrhizobium Nitrogen Fixation Symbiosis Phylogenetic Analysis

Unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neuropathology and behavioral deficits in parkinsonian rats withα-synucleinopathy

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Neural Regeneration Research 2024年第9期19卷 2057-2067页

作者： Bismark Gatica-Garcia Michael J.Bannon Irma Alicia Martínez-Dávila Luis O.Soto-Rojas David Reyes-Corona lourdes Escobedo Minerva Maldonado-Berny ME Gutierrez-Castillo Armando J.Espadas-Alvarez Manuel A.Fernandez-Parrilla Juan U.Mascotte-Cruz CP Rodríguez-Oviedo Irais E.Valenzuela-Arzeta Claudia Luna-Herrera Francisco E.Lopez-Salas Jaime Santoyo-Salazar Daniel Martinez-Fong Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Laboratorio de Patogénesis Molecular Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Red de Medicina para la Educación y Desarrollo y la Investigación Científica de Iztacala(Red MEDICI) Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Nanoparticle Therapy Institute AguascalientesMéxico Departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Escuela Nacional de Medicina y Homeopatía Instituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Fisiología Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Biología Molecular y Biotecnología Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoMéxico Departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa de Nanociencias y Nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Parkinsonism by unilateral,intranigralβ-sitosterolβ-D-glucoside administration in rats is distinguished in that theα-synuclein insult begins unilaterally but spreads bilaterally and increases in severity over time,thus replicating several clinical features of Parkinson’s disease,a typicalα-*** Nurr1 repressesα-synuclein,we evaluated whether unilateral transfected of rNurr1-V5 transgene via neurotensin-polyplex to the substantia nigra on day 30 after unilateralβ-sitosterolβ-D-glucoside lesion could affect bilateral neuropathology and sensorimotor deficits on day 30 *** study found that rNurr1-V5 expression but not that of the green fluorescent protein(the negative control)reducedβ-sitosterolβ-D-glucoside-induced ***,a bilateral increase in tyrosine hydroxylase-positive cells and arborization occurred in the substantia nigra and increased tyrosine hydroxylase-positive ramifications in the *** addition,tyrosine hydroxylase-positive cells displayed less senescence markerβ-galactosidase and more neuron-cytoskeleton markerβIII-tubulin and brain-derived neurotrophic factor.A significant decrease in activated microglia(positive to ionized calcium-binding adaptor molecule 1)and neurotoxic astrocytes(positive to glial fibrillary acidic protein and complement component 3)and increased neurotrophic astrocytes(positive to glial fibrillary acidic protein and S100 calcium-binding protein A10)also occurred in the substantia *** effects followed the bilateral reduction inα-synuclein aggregates in the nigrostriatal system,improving sensorimotor *** results show that unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neurodegeneration(senescence and loss of neuron-cytoskeleton and tyrosine hydroxylase-positive cells),neuroinflammation(activated microglia,neurotoxic astrocytes),α-synuclein aggregation,and sensorimotor *** neurotrophic astrocytes and

关键词： A1 astrocytes A2 astrocytes gene therapy microglia motor deficits nanoparticles neurodegeneration neuroinflammation senescence α-synuclein aggregates

Computational screening of transition metal-doped CdS for photocatalytic hydrogen production

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npj Computational Materials 2022年第1期8卷 2190-2198页

作者： Yuting Li Daniel Bahamon Mutasem Sinnokrot lourdes F.Vega Research and Innovation Center on CO_(2) and Hydrogen(RICH) Khalifa UniversityPO Box 127788Abu DhabiUAE Chemical Engineering Department.Khalifa University PO Box 127788Abu DhabiUAE Chemistry Department Khalifa UniversityPO Box 127788Abu DhabiUAE

A novel computational screening study of single transition metal(TM),TM-doped,and dual TMs-doped on CdS(110)surfaces via DFT calculations is presented,focusing on their stability and catalytic activity,searching for efficient photocatalysts for hydrogen *** based on key performance descriptors allowed to fine-tune the *** indicate that TM dopants can reduce the energy band gap and enhance impurity ***,Rh,and Pd were found to be the best dopants in TM-doped CdS,since their|ΔG_(H)|is 80%smaller compared to the pristine CdS ***,TM1-TM2-co-doped CdS catalysts show better performance for the hydrogen evolution reaction(HER)due to synergistic effects of the two TMs,where Co-Pt,Pd-Pt and Co-Rh co-doping CdS significantly reduced|ΔGH|to less than 0.1 *** point out four promising novel co-catalysts(i.e.,Co,Co-Pt,Co-Rh,Rh-Ag)with very good performance in HER,to be further explored in experimental studies.

关键词： surface transition allowed

Origin of Traditional Chinese Medicine in Cuba in the 19th Century from Its Main Exponents and Some Notable Medical Descendants in the 20th Century

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Chinese Medicine and Culture 2023年第4期6卷 367-376页

作者： lourdes Bárbara Alpizar Caballero lourdes de la Caridad Borges Oquendo Endocrinology Institute Department of ScienceTechnology and InnovationHavana 10400Cuba Faculty of Medical Sciences“Calixto García” Department of ScienceTechnology and InnovationHavana 10400Cuba

The Cuban people are made up of three major migratory currents, the Chinese are one of them. They brought their culture, the methods, and procedures of traditional Chinese medicine(TCM) in the 19th century. Few were able to return and so they created families in Cuba;some of their descendants dedicated themselves to medicine. In order to investigate the practices that were predecessors of TCM in Cuba in the 19th century, a qualitative phenomenological research was carried out, reviewing what was published by various sources, applying documentary analysis, logical historical analysis, abstraction, synthesis, and systematization of the results on the regularities of the work and human behavior of Chinese doctors in the Cuban 19th and 20th centuries. This made it possible to identify six Chinese doctors in the 19th century in Cuba who gave rise to the beginning of some practices of TCM in Cuba, and five from the 20th century, descendants of coolies who dedicated themselves to other specialties of medicine. It was found that despite their geographical and time disperse, they were all notorious for their outstanding professional and human behavior, with a trail of accumulated successes in achieving “almost the impossible” with the patient. They have left their mark on Cuban culture.

关键词： Chinese doctor Coolies Popular phrase Immigrants Professional behavior Human behavior History

Triple lines gold nanoparticle-based lateral flow assay for enhanced and simultaneous detection of Leishmania DNA and endogenous control

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Nano Research 2015年第11期8卷 3704-3714页

作者： lourdes Rivas Alfredo de la Escosura-Muniz Lorena serrano Laura Altet Olga Francino ArmandSanchez Arben Merkoci ICN2-Nanobioelectronics & Biosensors Group Institut Catala de Nanociencia i Nanotecnologia Campus UAB 08193 Bellaterra (Barcelona) Spain Autonomous University of Barcelona Campus UAB 08193 Bellaterra (Barcelona) Spain Vetgenomics Edifici Eureka Parc de Recerca UAB 08193 Bellaterra (Barcelona) Spain Centre for Research in Agricultural Genomics-CSIC-IRTA-UAB-UB Campus UAB 08193 Cerdanyola del Varies Catalonia Spain ICREA - Institucio Catalana de Recerca i Estudis A vancats 08010 Barcelona Spain

一个新奇三元组在狗血样品为利什曼原虫 infantum DNA 的察觉与提高的敏感衬里侧面流动的试金(LFA ) 被设计并且成功地适用。提高的 LFA 方法论利用结合到 polyclonal 的金 nanoparticle 标签(AuNPs ) 第二等的抗体，它认出 anti-FITC ... 详细信息

一个新奇三元组在狗血样品为利什曼原虫 infantum DNA 的察觉与提高的敏感衬里侧面流动的试金(LFA ) 被设计并且成功地适用。提高的 LFA 方法论利用结合到 polyclonal 的金 nanoparticle 标签(AuNPs ) 第二等的抗体，它认出 anti-FITC 抗体。第二等的抗体的 polyclonal 性质允许多重绑定到主要抗体，导致提高了 AuNP plasmonics 信号。而且，由放大的狗 18S rRNA 基因组成的内长的控制被介绍避免假 negatives。用这策略， 0.038 每 DNA 扩大反应(DNA 样品的 1 parasite/100 L ) 刺了利什曼原虫寄生虫被检测。LFA 的察觉限制被发现比常规技术的低。在摘要，我们的新奇 LFA 设计是能被扩大到几种另外的 biosensing 情形的一种通用、简单的察觉到的选择。

关键词： DNA检测同时检测原虫控制纳米金多克隆抗体金纳米粒子基础

Survival after curative pancreaticoduodenectomy for ampullary adenocarcinoma in a South American population:A retrospective cohort study

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World Journal of Gastrointestinal Surgery 2022年第1期14卷 24-35页

作者： Ramiro Manuel Fernandez-Placencia Paola Montenegro Melvy Guerrero Mariana serrano Emperatriz Ortega Mercedes Bravo lourdes Huanca Stéphane Bertani Juan Manuel Trejo Patricia Webb Jenny Malca-Vasquez Luis Taxa Alberto Lachos-Davila Juan Celis-Zapata Carlos Luque-Vasquez Eduardo Payet Eloy Ruiz Francisco Berrospi Hepato-Pancreato-Biliary Section Department of Abdominal SurgeryInstituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Medical Oncology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Pathology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru International Joint Laboratory of Molecular Anthopological Oncology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Unite Pharmacochim&Pharmacol Dev UMR152F-31062 ToulouseFrance Department of Radiation Oncology Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru Department of Abdominal Surgery Instituto Nacional de Enfermedades NeoplasicasLima 15038Peru

BACKGROUND Ampullary adenocarcinoma(AAC)is a rare neoplasm that accounts for only 0.2%of all gastrointestinal *** incidence rate is lower than 6 cases per million *** prognostic factors have been described for AAC and are associated with a wide range of survival ***,these studies have been exclusively conducted in patients originating from Asian,European,and North American *** To evaluate the histopathologic predictors of overall survival(OS)in South American patients with AAC treated with curative pancreaticoduodenectomy(PD).METHODS We analyzed retrospective data from 83 AAC patients who underwent curative(R0)PD at the National Cancer Institute of Peru between January 2010 and October 2020 to identify histopathologic predictors of *** Sixty-nine percent of patients had developed intestinal-type AAC(69%),23%had pancreatobiliary-type AAC,and 8%had other ***-one percent of patients were classified as Stage I,according to the AJCC 8 th *** occurred primarily in the liver(n=8),peritoneum(n=4),and lung(n=4).Statistical analyses indicated that T3 tumour stage[hazard ratio(HR)of 6.4,95%confidence interval(CI)of 2.5-16.3,P<0.001],lymph node metastasis(HR:4.5,95%CI:1.8-11.3,P=0.001),and pancreatobiliary type(HR:2.7,95%CI:1.2-6.2,P=0.025)were independent predictors of *** Extended tumour stage(T3),pancreatobiliary type,and positive lymph node metastasis represent independent predictors of a lower OS rate in South American AAC patients who underwent curative PD.

关键词： Gastrointestinal neoplasms Adenocarcinoma Ampulla Pancreaticoduodenectomy Survival South America

Frailty and acute coronary syndrome: does gender matter?

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Journal of Geriatric Cardiology 2019年第2期16卷 138-144页

作者： lourdes Vicent Manuel Martinez-Selles Servicio de Cardiologia Hospital General Universitario Gregorio MaraiionCIBERCVMadridSpain Universidad Europea Universidad ComplutenseMadridSpain

There are important sex-related differences in elderly patients with acute coronary syndrome(ACS).Women are older,more frequently frail,and present more comorbidities than *** symptoms at presentation are also more common in female patients,they are leaded to a delayed diagnosis and *** angiography and subsequent revascularization are frequently underused in elderly women and they tend to receive less guidelines-recommended *** prognosis in elderly frail women with ACS is poor,and it is with high mortality and readmissions *** is recurrent ischemic events in which it is more frequent in women than in *** time might be long,and a multidisciplinary approach is desirable to improve prognosis and quality of *** studies are needed in order to clarify the benefit of the different therapies in the group of frail women,and this is particularly true for revascularization,as scientific evidence in this group is very scarce.

关键词： Acute coronary syndrome Frailty The elderly Women