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Quantitative multiparametric magnetic resonance imaging can aid non-alcoholic steatohepatitis diagnosis in a Japanese cohort

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World Journal of Gastroenterology 2021年第7期27卷 609-623页

作者： Kento Imajo louise tetlow Andrea Dennis Elizabeth Shumbayawonda Sofia Mouchti Timothy J Kendall Eve Fryer Shogi Yamanaka Yasushi Honda Takaomi Kessoku Yuji Ogawa Masato Yoneda Satoru Saito Catherine Kelly Matt D Kelly Rajarshi Banerjee Atsushi Nakajima Department of Gastroenterology and Hepatology Yokohama City University School of MedicineYokohama 236-0004Japan Innovation PerspectumOxford OX42LLUnited Kingdom Centre for Inflammation Research University of EdinburghEdinburghUnited KingdomEdinburgh EH164TJUnited Kingdom Department of Cellular Pathology Oxford University Hospitals NHS Foundation TrustOxford OX39DUUnited Kingdom Anatomic and Clinical Pathology Department Yokohoma City University HospitalYokohoma 236-0004Japan Department of Gastroenterology Yokohama City University Graduate School of MedicineYokohama 236-0004Japan

BACKGROUND Non-invasive assessment of non-alcoholic steatohepatitis(NASH)is increasing in desirability due to the invasive nature and costs associated with the current form of assessment;liver *** multiparametric magnetic resonance imaging(mpMRI)to measure liver fat(proton density fat fraction)and fibroinflammatory disease[iron-corrected T1(cT1)],as well as elastography techniques[vibration-controlled transient elastography(VCTE)liver stiffness measure],magnetic resonance elastography(MRE)and 2D Shear-Wave elastography(SWE)to measure stiffness and fat(controlled attenuated parameter,CAP)are emerging alternatives which could be utilised as safe surrogates to liver *** To evaluate the agreement of non-invasive imaging modalities with liver biopsy,and their subsequent diagnostic accuracy for identifying NASH *** From January 2019 to February 2020,Japanese patients suspected of NASH were recruited onto a prospective,observational study and were screened using noninvasive imaging techniques;mpMRI with LiverMultiScan®,VCTE,MRE and *** were subsequently biopsied,and samples were scored by three independent *** diagnostic performances of the non-invasive imaging modalities were assessed using area under receiver operating characteristic curve(AUC)with the median of the histology scores as the gold standard *** between all three independent pathologists was further explored using Krippendorff’s alpha(a)from weighted kappa *** N=145 patients with mean age of 60(SD:13 years.),39%females,and 40%with body mass index≥30 kg/m2 were included in the *** identifying patients with NASH,MR liver fat and cT1 were the strongest performing individual measures(AUC:0.80 and 0.75 respectively),and the mpMRI metrics combined(cT1 and MR liver fat)were the overall best non-invasive test(AUC:0.83).For identifying fibrosis≥1,MRE performed best(AUC:0.97),compared to VCTE-liver stiffness measure(AU

关键词： Corrected T1 Fibro-inflammation Non-invasive imaging Non-alcoholic steatohepatitis Multiparametric magnetic resonance imaging Non-alcoholic fatty liver disease

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Technical Design for Holistic Wireless Communication in Smart Buildings

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Transactions of Nanjing University of Aeronautics and Astronautics 2018年第S1期35卷 81-87页

作者： Sean Rhys Jones Mark Gillott Rabah Boukhanouf Gavin Walker David tetlow Faculty of Engineering Department of Architecture and Built EnvironmentUniversity of Nottingham

This paper presents the design of a wireless building monitoring network implemented at the University of Nottingham's Creative Energy Homes test *** network is installed in seven smart buildings with the aim of holistically collecting energy *** will be used to inform a central control algorithm to optimise the energy flows between buildings,in turn promoting the smart cities *** and meters measuring temperature,humidity,CO_2,heat energy,power,and stratified tank temperature are ***,the communication protocols utilised are also discussed,which include wireless MBus and *** paper also covers the methods used for ensuring the reliability of data signals and the system controls.

关键词： smart cities smart buildings communication network building monitoring

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小麦胚乳14-3-3蛋白的表达及其与淀粉体淀粉合成酶的互作

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作物学报 2009年第8期35卷 1445-1450页

作者：宋健民戴双李豪圣刘爱峰程敦公楚秀生 Ian J tetlow Michael J EMES 山东省农业科学院作物研究所山东济南250100 University of Guelph GuelphN1G 2W1Canada

从小麦胚乳中克隆了14-3-3基因,并将其分别插入pET29c和pET41c质粒,用热激法转化大肠杆菌BL21-CodonPlus(DE3)-RP,得到高效表达的蛋白,但融合蛋白主要以包涵体的形式存在。可溶性的融合蛋白可直接通过S-蛋白琼脂糖树脂纯化。包涵体经8mo... 详细信息

从小麦胚乳中克隆了14-3-3基因,并将其分别插入pET29c和pET41c质粒,用热激法转化大肠杆菌BL21-CodonPlus(DE3)-RP,得到高效表达的蛋白,但融合蛋白主要以包涵体的形式存在。可溶性的融合蛋白可直接通过S-蛋白琼脂糖树脂纯化。包涵体经8molL-1尿素溶解变性,稀释复性后,结合到S-蛋白琼脂糖树脂上,也得到纯化的融合蛋白。复性后的融合蛋白对蔗糖合酶活性表现抑制作用,说明包涵体14-3-3融合蛋白恢复活性。将结合14-3-3融合蛋白的S-蛋白琼脂糖树脂作为诱饵与小麦胚乳淀粉体提取液进行亲和杂交,与14-3-3蛋白特异互作的淀粉合成酶结合到S-蛋白琼脂糖树脂上,Western检测结果表明,淀粉体淀粉合酶I(SSI)、淀粉合酶II(SSII)、淀粉分支酶Iia(SBEIIa)、淀粉分支酶Iib(SBEIIb)和ADP焦磷酸化酶大亚基(SH2)与14-3-3蛋白存在互作,而淀粉分支酶I(SBEI)、淀粉磷酸化酶(SP)、D-酶(DE)和ADP焦磷酸化酶小亚基(BT2)不能与14-3-3蛋白结合,说明小麦胚乳14-3-3蛋白对淀粉体淀粉合成具有一定的调控作用。

关键词：小麦14-3-3蛋白表达淀粉合成酶蛋白互作

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英国妇女妊娠期肝内胆汁淤积病例发生的ATP8B1突变

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世界核心医学期刊文摘（胃肠病学分册） 2005年第9期1卷 39-40页

作者： Müllenbach R. Bennett A. tetlow N. C. Williamson 雷小英 Maternal and Fetal Disease Group Imperial College London Hammersmith Campus Du Cane Road London W12 0NN United Kingdom Dr.

Background: Intrahepatic cholestasis of pregnancy (ICP) affects approximately 0.7%of pregnancies in the UK and is associated with prematurity, fetal distress, and intrauterine death. Homozygous mutations in the ATP8B1 gene cause cholestasis with a normal serum gamma-glutamyl transpeptidase (γ-GT), and have been reported in two forms of cholestasis: progressive familial intrahepatic cholestasis type 1 (PFIC1) and benign recurrent intrahepatic cholestasis (BRIC). Aims: To establish whether mutations in ATP8B1 are associated with ICP in British cases. Patients: Sixteen well phenotyped women with ICP without raised γ-GT were selected for sequence analysis. Subsequently, 182 patients and 120 controls were examined for the presence of the variants detected. Methods: All coding exons were sequenced in 16 cases. Eight ICP cases, including two women carrying a mutation, were investigated using in vivo hepatic 31P magnetic resonance spectroscopy (MRS). Results: Two heterozygous ATP8B1 transitions (208G >A and 2599C >T) that resulted in amino acid substitutions were identified; 208G >A was identified in three cases. MRS revealed an increased phosphodiester signal (Mann-Whitney U test, p = 0.03) and a decreased phosphomonoester/phosphodiester ratio (p = 0.04) in ICP cases compared with controls. Conclusions: We were able to demonstrate ATP8B1 mutations in ICP. MRS studies suggest that susceptibility to ICP is associated with a relative rise in biliary phospholipid. These data also suggest that MRS may be used for non-invasive assessment of the liver and biliary constituents in cholestasis.

关键词： ATP8B1 英国妇女肝内胆汁淤积胎死宫内健康孕妇胎儿窘迫子突基因突变无创性检测基因纯合

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