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Versatile multiplexed super-resolution imaging of nanostructures by Quencher-Exchange-PAINT

Nano Research 2018年第12期11卷 6141-6154页

作者： Tobias Lutz Alexander H.Clowsley Ruisheng Lin Stefano Pagliara lorenzo di michele Christian Soeller Living Systems Institute & Biomedical Physics University of ExeterExeter EX44QDUK Cavendish Laboratory University of CambridgeCambridge CB30HEUK

The optical super-resolution technique DNA-PAINT (Point Accumulation Imaging in Nanoscale Topography) provides a flexible way to achieve imaging of nanoscale structures at ∼10-nanometer resolution. In DNA-PAINT, fluorescently labeled DNA “imager” strands bind transiently and with high specificity to complementary target “docking” strands anchored to the structure of interest. The localization of single binding events enables the assembly of a super-resolution image, and this approach effectively circumvents photobleaching. The solution exchange of imager strands is the basis of Exchange-PAINT, which enables multiplexed imaging that avoids chromatic aberrations. Fluid exchange during imaging typically requires specialized chambers or washes, which can disturb the sample. Additionally, diffusional washout of imager strands is slow in thick samples such as biological tissue slices. Here, we introduce Quencher-Exchange-PAINT—a new approach to Exchange-PAINT in regular open-top imaging chambers—which overcomes the comparatively slow imager strand switching via diffusional imager washout. Quencher-Exchange-PAINT uses “quencher” strands, i.e., oligonucleotides that prevent the imager from binding to the targets, to rapidly reduce unwanted single-stranded imager concentrations to negligible levels, decoupled from the absolute imager concentration. The quencher strands contain an effective dye quencher that reduces the fluorescence of quenched imager strands to negligible levels. We characterized Quencher-Exchange-PAINT when applied to synthetic, cellular, and thick tissue samples. Quencher-Exchange-PAINT opens the way for efficient multiplexed imaging of complex nanostructures, e.g., in thick tissues, without the need for washing steps.

关键词： super-resolution microscopy fluorescence imaging DNA nanotechnology DNA-PAINT fluorescence quencher

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Thermodynamics and dielectric response of BaTiO_(3)by data-driven modeling

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npj Computational Materials 2022年第1期8卷 1998-2014页

作者： lorenzo Gigli Max Veit michele Kotiuga Giovanni Pizzi Nicola Marzari michele Ceriotti Laboratory of Computational Science and Modeling(COSMO) Institute of MaterialsÉcole Polytechnique Fédérale de LausanneCH-1015LausanneSwitzerland Theory and Simulation of Materials(THEOS)and National Centre for Computational Design and discovery of Novel Materials(MARVEL) École Polytechnique Fédérale de LausanneCH-1015LausanneSwitzerland

Modeling ferroelectric materials from first principles is one of the successes of density-functional theory and the driver of much development effort,requiring an accurate description of the electronic processes and the thermodynamic equilibrium that drive the spontaneous symmetry breaking and the emergence of macroscopic *** demonstrate the development and application of an integrated machine learning model that describes on the same footing structural,energetic,and functional properties of barium titanate(BaTiO_(3)),a prototypical *** model uses ab initio calculations as a reference and achieves accurate yet inexpensive predictions of energy and polarization on time and length scales that are not accessible to direct ab initio *** predictions allow us to assess the microscopic mechanism of the ferroelectric *** presence of an order-disorder transition for the Ti off-centered states is the main driver of the ferroelectric transition,even though the coupling between symmetry breaking and cell distortions determines the presence of intermediate,partly-ordered ***,we thoroughly probe the static and dynamical behavior of BaTiO_(3)across its phase diagram without the need to introduce a coarse-grained description of the ferroelectric ***,we apply the polarization model to calculate the dielectric response properties of the material in a full ab initio manner,again reproducing the correct qualitative experimental behavior.

关键词： transition ferroelectric dielectric

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Transient global amnesia and stroke: not that benign?

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Stroke & Vascular Neurology 2022年第2期7卷 92-93页

作者： michele Romoli lorenzo Muccioli Neurology and Stroke Unit Department of NeuroscienceMaurizio Bufalini HospitalCesenaItaly Neurology Clinic University of Perugia-S.Maria della Misericordia HospitalPerugiaItaly dipartimento di Scienze Biomediche e Neuromotorie Universitàdi BolognaBolognaItaly

After more than 50 years since Fisher and Adams’original description in 1964,1 transient global amnesia(TGA)is still a puzzling *** time,several mechanisms have been proposed for TGA pathogenesis,including venous congestion,cortical spreading depression,seizures and arterial ischaemia.2–4 A sufficient amount of studies in favour and against each of these mechanisms has emerged,fostering uncertainty regarding aetiology as well as *** this extent,most of the controversy stands on whether TGA represents a risk factor for stroke,or whether this is just as benign as originally thought.

关键词： arterial pathogenesis transient

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p-Multilevel Preconditioners for HHO discretizations of the Stokes Equations with Static Condensation

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Communications on Applied Mathematics and Computation 2022年第3期4卷 783-822页

作者： lorenzo Botti Daniele A.di Pietro Department of Engineering and Applied Sciences University of BergamoBergamoItaly IMAG Univ MontpellierCNRSMontpellierFrance

We propose a p-multilevel preconditioner for hybrid high-order(HHO)discretizations of the Stokes equation,numerically assess its performance on two variants of the method,and compare with a classical discontinuous Galerkin *** efficient implementa-tion is proposed where coarse level operators are inherited using L2-orthogonal projec-tions defined over mesh faces and the restriction of the fine grid operators is performed recursively and *** h-and k-dependency are investigated tackling two-and three-dimensional problems on standard meshes and graded *** the two HHO for-mulations,featuring discontinuous or hybrid pressure,we study how the combination of p-coarsening and static condensation influences the V-cycle *** particular,two dif-ferent static condensation procedures are considered for the discontinuous pressure HHO variant,resulting in global linear systems with a different number of unknowns and matrix non-zero ***,we show that the efficiency of the solution strategy might be impacted by static condensation options in the case of graded meshes.

关键词： Stokes equations divergence free constraint Hybrid high-order discontinuous Galerkin p-multigrid Static condensation

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Energy flow in negative index materials

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Chinese Optics Letters 2004年第7期2卷 428-430页

作者： lorenzo Bolla michele Midrio Carlo G.Someda The Istituto Nazionale per la Fisica della Materia (I. N. F. M.) Dipartimento di Ingegneria Elettrica Gestionale e Meccanica Universita degli Studi di Udine viale delle Scienze 208 33100 Udine Italy I. N. F. M. Dipartimento di Ingegneria dell'Informazione via G. Gradenigo 6/B 35131 Padova Italy

Prom Maxwell's equations, we compute the speed and the direction of propagation of active power refracted from air into a negative index material. We prove, both analytically and numerically that the power may refract positively even if phase fronts refract negatively. Considerations on the usage of ray optics in problems involving negative index materials are drawn.

关键词： Geometric optics Negative index materials

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The Ritz-Sublaminate Generalized Unified Formulation approach for piezoelectric composite plates

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International Journal of Smart and Nano Materials 2018年第1期9卷 34-55,I0002页

作者： michele D’Ottavio lorenzo Dozio Riccardo Vescovini Olivier Polit LEME UPLUniv Paris NanterreVille d’AvrayFrance Department of Aerospace Science and Technology Politecnico di MilanoMilanoItaly

This paper extends to composite plates including piezoelectric plies the variable kinematics plate modeling approach called Sublaminate Generalized Unified Formulation(SGUF).Two-dimensional plate equations are obtained upon defining a priori the through-thickness distribution of the displacement field and electric *** to SGUF,independent approximations can be adopted for the four components of these generalized displacements:an Equivalent Single Layer(ESL)or Layer-Wise(LW)description over an arbitrary group of plies constituting the composite plate(the sublaminate)and the polynomial order employed in each *** solution of the two-dimensional equations is sought in weak form by means of a Ritz *** this work,boundary functions are used in conjunction with the domain approximation expressed by an orthogonal basis spanned by Legendre *** proposed computational tool is capable to represent electroded surfaces with equipotentiality ***-vibration problems as well as static problems involving actuator and sensor configurations are *** case studies are presented,which demonstrate the high accuracy of the proposed Ritz-SGUF approach.A model assessment is proposed for showcasing to which extent the SGUF approach allows a reduction of the number of unknowns with a controlled impact on the accuracy of the result.

关键词： Piezoelectric composites variable kinematics model Ritz method unified formulation

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Breakthroughs and challenges in the management of pediatric viral hepatitis

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World Journal of Gastroenterology 2021年第20期27卷 2474-2494页

作者： Emanuele Nicastro lorenzo Norsa Angelo di Giorgio Giuseppe Indolfi lorenzo D'Antiga Department of Pediatric Gastroenterology Hepatology and Transplantation ASST Papa Giovanni XXIIIBergamo 24127Italy Department of Neurofarba Meyer Children's University Hospital of FlorenceFlorence 50137Italy

Chronic infections by hepatitis B virus(HBV)and hepatitis C virus(HCV)major causes of advanced liver disease and mortality *** regarded as benign infections in children,their persistence through adulthood is undoubtedly of *** advances in HCV treatment have restored the visibility of these conditions and raised expectations for HBV treatment,which is currently far from being *** we describe direct-acting antivirals available for pediatric HCV(sofosbuvir/ledipasvir,sofosbuvir/velpatasvir,glecaprevir/pibrentasvir)and their real-world use.A critical review of the HBV pediatric classification is ***-HBV investigational compounds are reviewed in light of the pathophysiology in the pediatric population,including capsid assembly modulators,antigen secretion inhibitors,silencing RNAs,and immune *** for screening and management of immunosuppressed children or those with other risk factors or comorbidities are also summarized.

关键词： Hepatitis C Hepatitis B direct acting antivirals Liver cirrhosis Children

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Circularly polarized luminescence of enantiopure carboline-based europium cryptates under visible light excitation

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Journal of Rare Earths 2020年第5期38卷 564-570页

作者： Carolin Dee Francesco Zinna Elisabeth Kreidt lorenzo Arrico Aurora Rodriguez-Rodriguez Carlos Platas-Iglesias lorenzo di Bari Michael Seitz University of Tübingen Institute of Inorganic ChemistryAuf der Morgenstelle 1872076TubingenGermany dipartimento di Chimica e Chimica Industriale Universita di PisaVia Moruzzi 1356124PisaItaly Centro de Investigacions Cientificas Avanzadas(CICA)and Departamento de Quimica Universidade da CorunaCampus da Zapateira-Rua da Fraga 1015008A CorunaSpain

The enantiomers of carboline-based cryptates were successfully resolved by chiral *** complexes show high configurational stability under harsh conditions and their absolute configuration was determined by comparing theoretical and expe rimental electronic circular dichroism *** e nantiopure cryptates exhibit strong circularly polarized luminescence with a maximum dissymmetry factor glum=0.25 for the f-f transition 5 D0→7 F1(λ=594 nm)under visible light excitation atλgx=400 nm.

关键词： Molecular lanthanoid complexes Lanthanoid luminescence Chirality Circularly polarized luminescence(CPL) Carboline Cryptates

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direct-fed microbial: Animal nutrition and reproduction responses

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Journal of Integrative Agriculture 2015年第3期14卷 494-496页

作者： Abdelfattah Z M Salem Nicolas di lorenzo Member of JIA Editorial Board Faculty of Veterinary Medicine and Animal Science Autonomous University of the State of Mexico North Florida Research and Education Center University of Florida Marianna Guest Editor Dr.

Manipulating the gastrointestinal microbial ecosystem to enhance animal performance and reproductive responses has been one of the main goals of animal science researchers and *** restrictions to the use of antimicrobials as growth promoters led researchers to seek alternative practices that can show promise both from the standpoint of efficacy as well as from the practical and economic *** of the alternatives that surfaced as very promising in the last few decades is the use of direct-fed microbials （DFM） as a means to modulate the effects of the gastrointestinal microbiome on the host immune status, health and productivity.

关键词： microbial nutrition reproduction goals promise ecosystem fermentation anaerobic digestion pleased

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Finding a way to preserve mitochondria: new pathogenic pathways in experimental multiple sclerosis

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Neural Regeneration Research 2019年第1期14卷 77-78页

作者： Andrea Mancini lorenzo Gaetani Lucia Gentili Massimiliano di Filippo Clinica Neurologica Dipartimento di Medicina Università degli Studi di Perugia

Multiple sclerosis(MS)is a chronic disorder affecting central nervous system(CNS)in which inflammatory and neuro-degenerative features coexist since the earlier phases of the disease(Reich et al.,2018).During last years,attention has been directed toward the possible pathogenic pathways linking these two different features characterizing MS,in order to acquire a better understanding of the disease pathogenesis and to design new disease modifying *** available therapies for MS are primarily

关键词：小径病原硬化多重试验性线粒体保存疾病

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