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Subcutaneous Model for the Study of dengue Virus Infection in Immune Competent Mice

Journal of Biosciences and Medicines 2018年第5期6卷 97-110页

作者： Beatriz Senra Santos Natalia Lima Pessoa Natalia Lucinda Gustavo Cardoso de oliveira Thais Souza Silva Ketyllen Reis Andrade Ketyllen Reis Andrade Bruno Galv&atilde o Filho Marcele neves Rocha Alexandre Vieira Machado Pedro Augusto Alves É rica Alessandra Rocha Alves Erna Geessien Kroon Marco Antô nio Campos Instituto René Rachou Funda&amp amp ccedil & amp atilde o Oswaldo Cruz Fiocruz Belo Horizonte Brazil departamento de Microbiologia Universidade Federal de Minas Gerais Belo Horizonte Brazil Empresa Brasileira de Pesquisa Agropecuá ria Laborat&amp amp oacute rio de Virologia Brasilia Brazil

Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent dengue virus (deNV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent deNV (deNV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously deNV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.

关键词： Subcutaneous Murine Model dengue Disease Immune Competent Mouse Cytokines Innate Immune Response

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根管内增强纤维在儿科的应用：病例报告

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中国口腔医学继续教育杂志 2005年第3期8卷 56-58页

作者： Rachel de oliveira Rocha Lucimara Teixeira das neves Noely Regina Marotti Marcia Turolla Wanderley Maria Salete Nahás Pires Corre^a 吴南(译) 赵玉鸣(校) 不详北京大学口腔医学院北京海淀区中关村南大街22号100081

本文介绍了一种使用间接树脂冠和根管内增强纤维来修复龋坏的上颌乳切牙的方法。每颗牙齿都先进行了根管治疗。使用根管内增强纤维的优点包括：树脂冠强度增加，透明，相对操作简便。另外，使用间接树脂冠能够获得很好的外形和美观，同... 详细信息

本文介绍了一种使用间接树脂冠和根管内增强纤维来修复龋坏的上颌乳切牙的方法。每颗牙齿都先进行了根管治疗。使用根管内增强纤维的优点包括：树脂冠强度增加，透明，相对操作简便。另外，使用间接树脂冠能够获得很好的外形和美观，同时能够减少儿童的椅旁时间。间接树脂冠和根管内增强纤维在一名3岁女孩的使用以图示说明这一技术。

关键词：根管增强纤维儿科上颌乳切牙牙齿

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Evaluation of Different Levels of Addition of Souari Nut Peel (Caryocar brasiliense) on the Quality of the Mombasa Grass Silage

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Journal of Agricultural Science and Technology(B) 2019年第5期9卷 356-364页

作者： Raísa Turcato de oliveira Andréia Santos Cezário Wallacy Barbacena Rosa dos Santos Jéferson Corrêa Ribeiro Tiago neves Pereira Valente Hellen Cardoso de oliveira departament of Animal Science Instituto Federal Goiano(IFGoiano)Campus Morrinhos75650-000Goiás StateBrasil department of Animal Science Instituto Federal Goiano(IFGoiano)Campus Posse73900-000Goiás StateBrasil

The objective of this study was to evaluate the effects of the addition of souari nut peel on the fermentative characteristics and chemical composition of the Mombasa grass silage.A completely randomized design with five levels of addition(0%,5%,10%,15%and 20%in natural matter basis)of souari nut peel with three replicates was applied in the *** silos were opened at 55 d after starting the ensiling *** data were analyzed by means of linear/quadratic regression analysis,according to the best fit of the data and the probability values to 5%,using the Statistical Analysis System(SAS,2002).There were a linear decreasing effect on the neutral detergent fiber(NDF),ash and increasing values for the effluent in the *** effect was observed for crude protein(CP),lignin and *** the other factors dry matter(DM),acid detergent fiber(ADF)and gases there was no influence on the addition of souari nut *** 15%addition level of souari nut peel increased CP content,decreased NDF and pH,which was recommended to improve the fermentation profile of Mombasa grass silage.

关键词： Additive effluent production fermentation quality

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Exercise training in hypertension:Role of microRNAs

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World Journal of Cardiology 2014年第8期6卷 713-727页

作者： Vander Josédas neves Tiago Fernandes Fernanda Roberta Roque Ursula Paula RenóSoci Stéphano Freitas Soares Melo Edilamar Menezes de oliveira Laboratory of Biochemistry and Molecular Biology of the Exercise School of Physical Education and SportUniversity of Sao PauloSao PauloSP 05508-900Brazil

Hypertension is a complex disease that constitutes an important public health problem and demands many studies in order to understand the molecular mechanisms involving his pathophysiology. Therefore, an increasing number of studies have been conducted and new therapies are continually being discovered. In this context, exercise training has emerged as an important non-pharmacological therapy to treat hypertensive patients, minimizing the side effects of pharmacological therapies and frequently contributing to allow pharmacotherapy to be suspended. Several mechanisms have been associated with the pathogenesis of hypertension, such as hyperactivity of the sympathetic nervous system and renin-angiotensin aldosterone system,impaired endothelial nitric oxide production, increased oxygen-reactive species, vascular thickening and stiffening, cardiac hypertrophy, impaired angiogenesis, and sometimes genetic predisposition. With the advent of microRNAs(miRNAs), new insights have been added to the perspectives for the treatment of this disease, and exercise training has been shown to be able to modulate the miRNAs associated with it. Elucidation of the relationship between exercise training and miRNAs in the pathogenesis of hypertension is fundamental in order to understand how exercise modulates the cardiovascular system at genetic level. This can be promising even for the development of new drugs. This article is a review of how exercise training acts on hypertension by means of specific miRNAs in the heart, vascular system, and skeletal muscle.

关键词： Exercise training Hypertension MicroRNA Heart Vascular system Macrocirculation Microcirculation Muscles Angiogenesis

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Residual Feed Intake as a Measure to Select Genetically Productive Beef Cattle: A Review

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Journal of Agricultural Science and Technology(B) 2020年第2期10卷 118-123页

作者： Tiago neves Pereira Valente Andréia Santos Cezário Wallacy Barbacena Rosa dos Santos Jeferson Corrêa Ribeiro Eliandra Maria Bianchini oliveira Erico da Silva Lima Bruno Borges deminicis Thasia Martins Macedo Rogério de Carvalho Veloso department of Animal Science Instituto Federal Goiano(IFGoiano)Campus PossePosse City73900-000Goiás StateBrasil department of Animal Science Instituto Federal Goiano(IFGoiano)Campus MorrinhosMorrinhos City75650-000Goiás StateBrasil department of Animal Science Faculdades Metropolitanas Unidas(FMU)São Paulo City05607-000São Paulo StateBrazil department of Animal Science Federal University of Southern Bahia(UFSB-BA)Teixeira de Freitas City45613-204Bahia StateBrazil

Measures such as residual feed intake(RFI)have been increasingly considered to select cattle with superior feed efficiency,as it is a characteristic of feed efficiency calculated as the difference between observed intake and estimated intake considering metabolic weight and the weight *** feed intake is obtained through the multiple regression equation of observed consumption on metabolic mean live weight and weight *** standard was shared among animals into one herd selected for low RFI(high efficiency)and compared to the one selected for high RFI(low efficiency).There was no correlated response in weight per year and in weight gain,and the effectiveness of low RFI selection in reducing feed costs was proven without affecting ***,selection of animals with negative RFI is not done by selecting animals directly according to the size of the viscera,rather it is done indirectly,that is,when selecting the most efficient animals and normally these animals already have the smallest viscera *** RFI is a trait that presents genetic variability,selectable to improve and has moderate heritability,between 0.30 and *** RFI is important because it reduces the direct economic impacts of reduced feed intake,as well as the effects of environmental impact on reducing methane emissions from enteric fermentation of ruminants.

关键词： Feed conversion feed efficiency enteric fermentation

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Prevalence of Kudoa in Fish Fillets Caught in Para State

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Journal of Zoological Research 2020年第3期2卷 9-14页

作者： Marianna Vaz RODRIGUES Patrícia Tidori MIURA Jéssica Fernandes de oliveira Maria das Dores Correia Palha João Pessoa Araújo JÚNIOR UNESP-São Paulo State University(Unesp) BiotechnologyAlameda das Tecomarias s/n18607-440BotucatuSão PauloBrazil Food Technology Laboratory Food Science DepartmentUniversity of CampinasCidade Universitária“Zeferino Vaz”s/n13083-862CampinasSão PauloBrazil Universidade Federal Rural da Amazônia Avenida Presidente Tancredo Neves 250166077-830BelémParáBrazil

Kudoa is a myxozoan that causes myoliquefaction in marine *** of species only affect fish,but a *** outbreak was reported in 358 *** many species of Kudoa are known,none was described in Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus or Oxydoras niger until *** to the economic cost of eliminating seafood presenting myxozoan lesions,this study aimed to describe lesions found at necropsy and histopathology,as well as to detect this myxozoan by molecular *** this purpose,were sampled 85 fish of the following species:Brachyplatystoma filamentosum,Brachyplatystoma rousseauxii,Mugil curema,Plagioscion squamosissimus,and Oxydoras niger from Colares and Vigia,Pará,*** were carried out to describe lesions and molecular techniques(PCR and sequencing)were applied for *** muscle lesions were not observed at necropsy,histopathology revealed bacterial colonies,coagulative necrosis,dystrophic calcification,eosinophils,hemorrhage,parasitic pseudocysts,protozoan,and *** sequencing,***(GENBANK:LC128646)was identified as the most similar causative agent of fishes infection,but due to phylogenetic results and identity we suggest that the myxozoan found could be a new ***,high parasitism of this myxozoan was observed in fishes sampled,i.e.,90%in Colares and 100%in Vigia.

关键词： Myxozoan Parasitism Seafood Sanitary inspection Myoliquefaction

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Chemical Exposure Hazardous for Fish Hyphessobrycon eques through the Incorrect Release of Oil in Amazon Region

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Journal of Fisheries Science 2020年第2期2卷 19-23页

作者： Natalino da Costa Sousa Arthur dos Santos da Silva Keber Santos Costa Junior Francisco Alex Lima Barros Geisy Correa de oliveira Pedro Rodrigo Nery de Souza Claudmilson Rodrigues de Mesquita Bruna de jesus Santos Alexander Damaceno Lima santos Winnícius Muniz dos Santos Sá Nayara Siqueira dos Santos Márcia Valéria Silva do Couto Federal University of Pará(UFPA) Campus BragançaAlameda Leandro RibeiroBragança68600-000PABrazil Federal University of Pará(UFPA) Campus de TucuruíRua Itaipu36-Vila PermanenteTucuruí68464-000PABrazil Federal University of de Sergipe(UFS) Av.Marechal Rondons/n-Jardim Rosa ElzeSão Cristóvão49100-000SEBrasil Faculdade Pio Décimo Av.Pres.Tancredo neves 5655-Jabotiana49095-000Aracaju–SE.Brazil

Incorrect discard of oil used by fishing ship in the brazilian north region has become a common *** the possible hazard to the aquatic organisms,still missing scientific data about their ***,this study aimed to evaluate the stress and lethality caused by lubricant oil(FSAOLU)on fish Hyphessobrycon ***,it was used six different concentration diluted in water(0,22,24,26,28,30 and 32%of oil)and three replaces during 96 *** the end or during the experiment(with dying fish),it was collected blood samples(cutting the caudal fin)to determine physiological *** FSAOLU showed mean lethal dose(LD50-96h)of 27.36%,classified as toxic causing alterations in glucose values from the 26%.The greater dilution of FSAOLU(32%)increased 115%in glucose values when compared to the ***,lubricant oil when discarded in water,it present hazard to the aquatic organisms causing stress and mortality for fish being necessary adequate management to discard of this residue.

关键词： Toxicity FSA Sensibility Stress

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