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Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons

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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu Deng Yaoyao Liu Kui Zhang Shiya Lv longze sha Qi Xu Xinxia Cai Jinping Luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural *** chip was designed based on the specific structure of neurons and the requirements for detection and ***-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic *** employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated *** hippocampal neurons were cultured on the chip,forming a well-defined neural *** electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode *** study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microfluidic Microelectrode array In vitro Neural chip

Comparison ofβ-Amyloid Plaque Labeling Methods:Antibody Staining,Gallyas Silver Staining,and Thioflavin-S Staining

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Chinese Medical Sciences Journal 2018年第3期33卷 167-173页

作者： Xinze Shi Xuan Wei longze sha Qi Xu State Key Laboratory of Medical Molecular Biology and Neuroscience Center Institute of Basic Medical Sciences Chinese Academy of Medical Sciences&School of Basic Medicine Peking Union Medical CollegeBeijing 100005China High School Attached to Beijing University of Technology Beijing 100022China

Objective To evaluate senile plaque formation and compare the sensitivity of three differentβ-amyloid(Aβ)labeling methods(antibody staining,Gallyas silver staining,and thioflavin-S staining)to detect Aβ*** APPswe/PSEN1dE9 transgenic mice(APP/PS1)of different ages were used to examine spatiotemporal changes in Aβplaque *** staining,Gallyas silver staining,and thioflavin-S staining were used to detect Aβplaque deposition in the same brain region of adjacent slices from model mice,and the results were *** With aging,Aβplaques first appeared in the cortex and then the deposition increased throughout the whole *** greater plaque deposition was detected by 6E10 antibody than that analyzed with Gallyas silver staining or thioflavin-S staining(P<0.05).Plaque deposition did not show significant difference between the APP/PS1 mice brains assayed with Gallyas silver staining and ones with thioflavin-S staining(P=0.0033).Conclusions The APP/PS1 mouse model of Alzheimer’s disease could mimick the progress of Aβplaques occurred in patients with Alzheimer’s *** detection of Aβdeposition may be more sensitive than chemical staining methods.

关键词： β-amyloid plaques Alzheimer’s disease antibody staining Gallyas silver thioflavin-S

Study of Notch signaling activated in temporal lobe epilepsy and the anticonvulsant effects of DAPT treatment on seizures in a mouse model

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Study of Notch signaling activated in temporal lobe epilepsy...

生命的分子机器及其调控网络——2012年全国生物化学与分子生物学学术大会

作者： longze sha Xiaofeng Wu Yuan Yao Xiaoliang Xing Zhiqiang sha Xueqin wang Wanchen dou Liri Jin Yan Shen Liwen Wu Qi Xu Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical College Hospital Chinese Academy of Medical Sciences

The hypothesis that the elevated ra-tio of hippocampal neuronal excitation to inhibition may give rise to seizure has been widely accepted,but little is known about the involved *** work has sug-gested that Notch is associated to synaptic *** signaling was up-regulated in response to neuronal activity;mice with conditional deletion of Notch1 in CA1 pyramidal neurons of hippocampus exhibited a low magnitude of long-term potentiation that indic-ates impaired neuronal firing due to disruption of Notch1 *** this study,we found that Notch signaling was

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以失用症为突出表现的克雅病一例

中华神经科杂志 2021年第11期54卷 1181-1186页

作者：王洁牛婧雯沙龙泽孙鹤阳褚姗姗高晶中国医学科学院北京协和医院疑难重症及罕见病国家重点实验室100730 中国医学科学院北京协和医院神经科 100730 中国医学科学院基础医学研究所北京100730

分析1例2020年8月在中国医学科学院北京协和医院神经科住院的克雅病患者的临床表现和检查结果,并对其进行全面的神经心理学评估和失用相关评估。该患者为65岁男性,隐匿起病,症状逐渐进展,临床表现为失用症、快速进展的痴呆和锥体外系症... 详细信息

分析1例2020年8月在中国医学科学院北京协和医院神经科住院的克雅病患者的临床表现和检查结果,并对其进行全面的神经心理学评估和失用相关评估。该患者为65岁男性,隐匿起病,症状逐渐进展,临床表现为失用症、快速进展的痴呆和锥体外系症状,头颅磁共振成像提示双侧大脑半球"花边征",脑脊液14-3-3蛋白阳性,符合很可能克雅病的诊断标准,其失用症为典型的观念运动性失用。经复习文献,发现失用也可以是克雅病的主要神经心理损害表现,临床诊治中需要重视失用症的规范和正确评价、及时识别。

关键词：克-亚综合征失用症念动性病例报告

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散发型克雅病脑脊液tau蛋白的改变

中华神经科杂志 2020年第1期53卷 25-30页

作者：黄欣莹毛晨晖沙龙泽刘彩燕董立羚周雁李洁雷聃张梦雨沈东超李芹褚姗姗许琪彭斌崔丽英高晶中国医学科学院北京协和医院神经科北京100730 北京协和医学院北京100730 中国医学科学院基础医学研究所北京100730 中国医学科学院神经科学中心北京100730

目的探索脑脊液标志物,尤其是总tau蛋白(T-tau)及磷酸化tau蛋白(P-tau)对Creutzfeldt-Jakob病(Creutzfeldt-Jakob disease,CJD)的诊断与鉴别诊断价值。方法按2009年Brain标准及2018年Neurology改良版标准选取2018—2019年北京协和医院脑脊液生物标志物研究队列中的散发性CJD(sporadic CJD,sCJD)患者。按2011年美国国立老化研究所与阿尔茨海默病(AD)协会标准选择年龄、性别相对匹配的AD患者以及认知功能正常的其他神经系统疾病患者作为对照病例,应用其脑脊液标本进行分组对照研究。共收集了12例sCJD患者入组,同时收集49例AD患者以及14名认知正常者入对照组。所有脑脊液标本均为腰椎穿刺时直接滴落收集,-80℃保存,由专门实验室及指定实验员进行T-tau蛋白、P-tau蛋白测定。同时收集sCJD患者影像学及脑电图资料,并按规定将sCJD病例报送中国疾病预防控制中心,检测14-3-3蛋白及相关的基因。结果通过非参数秩和检验对比3组人群脑脊液tau蛋白含量,结果显示sCJD组T-tau水平达1211(448,2227)pg/ml,AD组T-tau水平达549(314,1078)pg/ml,均显著高于对照组[127(79,192)pg/ml,U=20、73,P<0.01]。sCJD组的T-tau蛋白水平升高更明显,显著高于AD患者(U=178,P=0.0349)。在AD患者中,P-tau水平达72(58,109)pg/ml,较对照组[27(15,42)pg/ml]显著升高(U=82,P<0.01),但在sCJD患者[32(24,47)pg/ml]无明显升高。sCJD患者的T-tau/P-tau比值[29.77(20.01,54.53)]高于AD[7.45(4.79,10.43);U=87,P<0.01]。12例sCJD头颅MRI均存在"花边征",5例存在脑电图三相波改变。9例sCJD患者被上报至中国疾病防治中心,其中4例14-3-3蛋白阳性;9例朊蛋白PRNP基因均无突变,129位密码子均为M/M型,219位密码子均为E/E型。结论sCJD患者脑脊液tau蛋白、T-tau/P-tau显著增高,对于sCJD的诊断及鉴别具有一定价值。

关键词：克-亚综合征痴呆生物学标记神经变性疾病