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3D printed silk-gelatin hydrogel scaffold with different porous structure and cell seeding strategy for cartilage regeneration

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Bioactive Materials 2021年第10期6卷 3396-3410页

作者： Qingtao Li Sheng Xu Qi Feng Qiyuan Dai longtao yao Yichen Zhang Huichang Gao Hua Dong Dafu Chen Xiaodong Cao National Engineering Research Center for Tissue Restoration and Reconstruction(NERC-TRR) South China University of TechnologyGuangzhouGuangDong510641China Department of Biomedical Engineering School of Material Science and EngineeringSouth China University of TechnologyGuangzhouGuangDong510641China School of Medicine South China University of TechnologyGuangzhouGuangDong510641China Key Laboratory of Biomedical Engineering of Guangdong Province Key Laboratory of Biomedical Materials and Engineering of the Ministry of EducationInnovation Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhouGuangDong510641China Laboratory of Bone Tissue Engineering Beijing Laboratory of Biomedical MaterialsBeijing Research Institute of Orthopaedics and TraumatologyBeijing JiShuiTan HospitalBeijing100035China Zhongshan Institute of Modern Industrial Technology of SCUT ZhongshanGuangdong528437China

Hydrogel scaffolds are attractive for tissue defect repair and reorganization because of their human tissue-like ***,most hydrogels offer limited cell growth and tissue formation ability due to their submicron-or nano-sized gel networks,which restrict the supply of oxygen,nutrients and inhibit the proliferation and differentiation of encapsulated *** recent years,3D printed hydrogels have shown great potential to overcome this problem by introducing macro-pores within *** this study,we fabricated a macroporous hydrogel scaffold through horseradish peroxidase(HRP)-mediated crosslinking of silk fibroin(SF)and tyramine-substituted gelatin(GT)by extrusion-based low-temperature 3D *** physicochemical characterization,we found that this hydrogel has excellent structural stability,suitable mechanical properties,and an adjustable degradation rate,thus satisfying the requirements for cartilage *** suspension and aggregate seeding methods were developed to assess the inoculation efficiency of the ***,the chondrogenic differentiation of stem cells was *** cells in the hydrogel differentiated into hyaline cartilage when the cell aggregate seeding method was used and into fibrocartilage when the cell suspension was ***,the effect of the hydrogel and stem cells were investigated in a rabbit cartilage defect *** implantation for 12 and 16 weeks,histological evaluation of the sections was *** found that the enzymatic cross-linked and methanol treatment SF5GT15 hydrogel combined with cell aggregates promoted articular cartilage *** summary,this 3D printed macroporous SF-GT hydrogel combined with stem cell aggregates possesses excellent potential for application in cartilage tissue repair and regeneration.

关键词： Silk hydrogel 3D printing Enzymatic cross-linking Cell aggregate seeding Cartilage regeneration

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大型凹球面数控加工工艺研究

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制造技术与机床 2013年第8期 110-113,160页

作者：姚龙涛中信重工机械股份有限公司河南洛阳471039

以压力杆和支座两类零件的加工为例,分析了大型凹球面的数控加工方法。选择以铣代车的加工工艺方案,对不同的刀具、走刀路线进行了分析比较,得到合理的铣削加工方案,并进行了实际加工验证。结果表明:采用合理的以铣代车工艺可提高生产... 详细信息

以压力杆和支座两类零件的加工为例,分析了大型凹球面的数控加工方法。选择以铣代车的加工工艺方案,对不同的刀具、走刀路线进行了分析比较,得到合理的铣削加工方案,并进行了实际加工验证。结果表明:采用合理的以铣代车工艺可提高生产效率和零件加工表面的质量,降低加工成本。

关键词：凹球面以铣代车走刀路线残留峰高

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Crustal activities recorded incoral reefs in the northwestern South China Sea

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Chinese Science Bulletin 2006年第B12期51卷 89-94页

作者： ZHAN Wenhuan yao Yantao ZHANG Zhiqiang SUN Zongxun ZHAN Meizhen SUN longtao LIU Zaifeng CAS Key Laboratory of Marginal Sea Geology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China

Coral reefs in the northwest of South China Sea have recorded the information from not only the environmental variation but also the crustal activities there during their development. The main crustal activities correlated with the coral reef development include fault, seismic, and volcano activities, etc. The high-resolution spark seismic profiles in the northwestern South China Sea show that the fault activities in the coral reef region have been clearly recorded, and appear as neonatal faults incising reefs. Earthquakes in the coral reef region are rather intense, especially the two occurring on December, 31, 1994, and January, 10, 1995, around the southwest of Leizhou Peninsula, with the magnitude of 6.1 and 6.2, respectively. They have great influence on the growth of the local coral reefs. Quaternary volcanos are active in the northwestern South China Sea, especially around the southwest of Leizhou Peninsula, and they have obvious control of the coral reef development. Some submarine volcanoes form the substrates of coral reef, while a few emerge above the sea surface and form coral islands.

关键词：南海西北部海域珊瑚礁外壳活动性环境变化

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鹅源副粘病毒SF02株的生物学特性研究

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上海农业学报 2004年第2期20卷 109-113页

作者：胡永强单松华何水林姚龙涛上海出入境检验检疫局上海200135 上海市奉贤区畜牧兽医站上海201400

研究鹅源副粘病毒SF0 2株的生物学特性 ,结果表明 :毒株的血凝素热稳定性指数为 3min ,血凝为快速解脱型 ;能凝集鸡、鸭、鼠、鸽、鹅、人 (O型和A型 )的红细胞 ,对绵羊和山羊的红细胞略凝 ,不凝集牛和猪的红细胞 ;病毒SF0 2株对胰蛋白... 详细信息

研究鹅源副粘病毒SF0 2株的生物学特性 ,结果表明 :毒株的血凝素热稳定性指数为 3min ,血凝为快速解脱型 ;能凝集鸡、鸭、鼠、鸽、鹅、人 (O型和A型 )的红细胞 ,对绵羊和山羊的红细胞略凝 ,不凝集牛和猪的红细胞 ;病毒SF0 2株对胰蛋白酶、氯仿、乙醚、pH3.0敏感 ,二价镁离子对病毒有保护作用 ;0 .0 1%的甲醛溶液既能灭活病毒 ,又能保持较好的抗原活性 ;鸡胚平均致死时间 (MDT)为 5 4h ,1日龄雏鸡脑内致病指数 (ICPI)为 1.88;实验感染草鹅、白皇鸽、七彩山鸡、鹧鸪、番鸭等 10 0 %发病与死亡 ,对杂交黄鸡、鹌鹑也有 5 0 %以上的致死率 ,对绿头野鸭则无死亡 ;这些结果为全面了解病毒的特性提供了一些科学依据。

关键词：鹅源副粘病毒 SF02株生物学特性血凝素热稳定性指数抗原活性

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Upconversion nanoparticles regulated drug&gas dual-effective nanoplatform for the targeting cooperated therapy of thrombus and anticoagulation

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Bioactive Materials 2022年第12期7卷 91-103页

作者： Shichen Liu yao Sun Teng Zhang longtao Cao Zhiwei Zhong Haoxin Cheng Qingqing Wang Zhuang Qiu Weimin Zhou Xiaolei Wang Department of Vascular Surgery The Second Affiliated Hospital of Nanchang UniversityNanchang UniversityNanchangJiangxi330006PR China The National Engineering Research Center for Bioengineering Drugs and the Technologies Institute of Translational MedicineNanchang UniversityNanchangJiangxi330088PR China College of Chemistry Nanchang UniversityNanchangJiangxi330088PR China School of Public Health&Jiangxi Provincial Key Laboratory of Preventive Medicine NanchangJiangxi330088PR China

Thromboembolism is the leading cause of cardiovascular ***,for the lack of targeting,short half-life,low bioavailability and high bleeding risk of the classical thrombolytic drugs,pharmacological thrombolysis is usually a slow process based on *** addition,frequently monitoring and regulating coagulation functions are also required during(and after)the process of *** address these issues,a targeted thrombolytic and anticoagulation nanoplatform(UCATS-UK)is developed based on upconversion nanoparticles(UCNPs)that can convert 808 or 980 nm near-infrared(NIR)light into UV/blue *** nanoplatform can target and enrich in the thrombus *** thrombolysis and anticoagulation therapy thus could be realized through the controlled release of urokinase(UK)and nitric oxide(NO).Both in vitro and in vivo experiments have confirmed the excellent thrombolytic and anticoagulative capabilities of this multifunctional *** with the unique fluorescent imaging capability of UCNPs,this work is expected to contribute to the development of clinical thrombolysis therapy towards an integrated system of imaging,diagnosis and treatment.

关键词： Targeted drug delivery Upconversion nanoparticles Controlled release Thrombus Anticoagulation

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乙型脑炎减毒株活疫苗对预防母猪流产的进一步观察

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微生物学报 1981年第2期 174-179页

作者：敖坚俞永新武佩芬张国铭姚龙涛朱全忠李河民卫生部药品生物制品检定所上海市奉贤县畜牧兽医站

1.以BHK21细胞系为基质生产的乙脑14-2株活疫苗,经免疫孕猪后,对母猪本身无任何不良反应,对仔猪亦无不良影响,证明该活疫苗对猪是安全的。 2.乙脑减毒活疫苗免疫猪后,产生乙脑中和抗体显著,阳转率为80—100%。14-2株的抗体阳转率和几何... 详细信息

1.以BHK21细胞系为基质生产的乙脑14-2株活疫苗,经免疫孕猪后,对母猪本身无任何不良反应,对仔猪亦无不良影响,证明该活疫苗对猪是安全的。 2.乙脑减毒活疫苗免疫猪后,产生乙脑中和抗体显著,阳转率为80—100%。14-2株的抗体阳转率和几何均值均较5-3株高。 3.免疫母猪的死产率显著下降,据统计,头胎母猪的死产率一般为47.3—58.3%,经乙脑活疫苗免疫后降低到6.6—8.1%,效果十分显著。其中14-2株较5-3株为优,当疫苗稀释至1000倍时仍有较好的保护作用。

关键词：母猪疫苗免疫中和指数保护力产活仔率减毒活疫苗乙脑病毒减毒株乙型脑炎

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Piceatannol enhances Beclin-1 activity to suppress tumor progression and its combination therapy strategy with everolimus in gastric cancer

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Science China(Life Sciences) 2023年第2期66卷 298-312页

作者： longtao Huangfu Xiaoyang Wang Shanshan Tian Junbing Chen Xueying Wang Biao Fan Qian yao Gangjian Wang Cong Chen Jing Han Xiaofang Xing Jiafu Ji Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education) Division of Gastrointestinal Cancer Translational Research LaboratoryPeking University Cancer Hospital&InstituteBeijing 100142China Department of Pharmacy Peking University Cancer Hospital&InstituteBeijing 100142China National Institute on Drug Dependence Peking UniversityBeijing 100191China Gastrointestinal Cancer Center Peking University Cancer Hospital&InstituteBeijing 100142China Department of Pathology Peking University Cancer Hospital&InstituteBeijing 100142China

The effects and regulation of Beclin-1-an autophagy-related protein-have not been fully defined, however, a negative correlation has been reported between Beclin-1 expression and carcinogenesis. Meanwhile, no compound has been shown to directly inhibit its activity. Here, we evaluate piceatannol, a naturally occurring polyphenolic compound, as a potential targeting agonist of Beclin-1, to assess its efficacy as an antitumor agent against gastric cancer. More specifically, we determine the effects of piceatannol treatment on cell viability using a monitoring system and colony forming assay. Piceatannol was found to efficiently inhibit the proliferation of several human gastric cancer cell lines. Autophagic flux is increased by piceatannol treatment, and correlates with inhibition of cell proliferation and colony formation. Additionally, microscale thermophoresis and surface plasmon resonance results show a direct interaction between piceatannol and Beclin-1, which reduces the phosphorylation activity of Beclin-1 at the Ser-295 site. Notably, piceatannol impairs the binding of Beclin-1 to Bcl-2 and enhances the recruitment of binding of UV radiation resistance-associated gene protein, which further triggers Beclin-1-dependent autophagy signaling. An increase in autophagic activity via treatment with the mTOR inhibitor, everolimus, effectively sensitizes piceatannol-induced antitumor effects. Xenograft models confirmed that piceatannol inhibits tumor development and elicits a potent synergistic effect with everolimus in vivo. Taken together, the findings of this study strongly support the application of combinatorial piceatannol and everolimus therapy in future clinical trials for gastric cancer patients.

关键词： piceatannol Beclin-1 autophagy everolimus gastric cancer drug synergy

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鹅副粘病毒上海株的分子鉴定

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上海农业学报 2002年第4期18卷 70-73页

作者：单松华邹键姚龙涛胡永强何水林龚祖埙上海出入境检验检疫局上海200135 中国科学院上海生命研究院生物化学与细胞生物学研究所上海200031 上海市奉贤区畜牧兽医站奉贤201400

对新近分离的鹅副粘病毒SF0 2株采用RT PCR方法 ,扩增部分F基因进行测序 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。通过对其F基因与我国大陆新城疫强毒F4 8E9株、疫苗毒Lasota株比较 ,发现该毒株的F基因... 详细信息

对新近分离的鹅副粘病毒SF0 2株采用RT PCR方法 ,扩增部分F基因进行测序 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。通过对其F基因与我国大陆新城疫强毒F4 8E9株、疫苗毒Lasota株比较 ,发现该毒株的F基因已发生了较大的变异 ,而与1999年在我国台湾省流行的新城疫病毒基因Ⅶ型有很高的同源性。

关键词：上海株分子鉴定鹅副粘病毒新城疫病毒 F基因序列分析 RT-PCR

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无杆式气缸弹射装置内弹道仿真与优化设计

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振动与冲击 2017年第6期36卷 122-127,198页

作者：姚琳马大为任杰魏龙涛李亚南京理工大学机械工程学院南京210094 航天长征国际贸易有限公司北京100039

提出一种新型无杆式气缸导弹弹射装置,可实现小尺寸、长行程、低过载发射。引入真实气体状态方程—RK状态方程,推导真实气体条件下比热力学能、比焓等热力学参数表达式,建立考虑真实气体效应的无杆式气缸弹射装置内弹道模型,获得了导弹... 详细信息

提出一种新型无杆式气缸导弹弹射装置,可实现小尺寸、长行程、低过载发射。引入真实气体状态方程—RK状态方程,推导真实气体条件下比热力学能、比焓等热力学参数表达式,建立考虑真实气体效应的无杆式气缸弹射装置内弹道模型,获得了导弹弹射过程中热力学参数与导弹运动参数的变化规律。计算结果表明:与"单电磁阀"供气方案相比,采用优化后的"主副电磁阀"供气方案,在弹射结束时,气体质量流量增大52.9%,低压室压强提高14.3%,导弹过载系数提高了41.3%;在最大过载和有效推弹行程不变的条件下,导弹的离轨速度提高15.8%。

关键词：导弹冷弹射无杆式气缸真实气体效应 “主副电磁阀”供气内弹道优化

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基于主题与情感联合预训练的虚假评论检测方法

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计算机研究与发展 2021年第7期58卷 1385-1394页

作者：张东杰黄龙涛张荣薛晖林俊宇路瑶阿里巴巴集团北京100102 中国科学院信息工程研究所北京100093 廊坊职业技术学院河北廊坊065001

商品评论信息是用户线上决策的重要依据,但在利益的驱使下商家往往会通过雇佣专业的写手撰写大量虚假评论的方式来误导用户,进而达到包装自己或诋毁竞争对手的目的.这种现象会造成不正当的商业竞争和极差的用户体验.针对这一现象,我们... 详细信息

商品评论信息是用户线上决策的重要依据,但在利益的驱使下商家往往会通过雇佣专业的写手撰写大量虚假评论的方式来误导用户,进而达到包装自己或诋毁竞争对手的目的.这种现象会造成不正当的商业竞争和极差的用户体验.针对这一现象,我们通过情感预训练的方法对现有的虚假评论识别模型进行了改进,并提出了一种能够同时整合评论语义和情感信息的联合预训练学习方法.鉴于预训练模型强大的语义表示能力,在联合学习框架中采用了2种预训练模型编码器分别用于抽取评论的语义和情感上下文特征,并通过联合训练的方法整合2种特征,最后使用Center Loss损失函数对模型进行优化.在多个公开数据集和多个不同任务上进行了验证实验,实验表明提出的联合模型在虚假评论检测与情感极性分析任务上都取得了目前最好的效果且具有更强的泛化能力.

关键词：虚假评论检测预训练模型情感分析联合训练 Center Loss

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