检索结果-南通市图书馆

Integrative analysis of transcriptomic profile reveals potential roles of miRNAs in regulating development of Marsupenaeus japonicas

引用

Journal of Oceanology and Limnology 2024年第1期42卷 201-215页

作者： Jing WANG longjun pu Xiaojuan ZHANG Cuicui LIANG Dandan DONG Jiantao GUAN Huarong GUO Ministry of Education Key Laboratory of Marine Genetics and Breeding College of Marine Life SciencesOcean University of ChinaQingdao 266003China Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China

Regulation of microRNAs(miRNAs)on various biological processes has been a surprising and exciting *** of miRNAs is the first step to comprehensively understand their ***,attempts on global identification and functional verification of miRNAs are very limited in penaeid shrimp Marsupenaeus japonicus,an economically important aquatic *** performing an integrated analysis of transcriptomic profile from gastrula embryos of ***,21conserved miRNAs in ***(mja-miRNAs),belonging to 19 miRNA families,were identified and *** the 21 mja-miRNAs,15 miRNAs were successfully verified to be predominantly expressed in gastrula stage,where they displayed dynamic expression patterns compared with those in naupliuin *** on perfect or near-perfect match to target region,120 target genes were predicted at transcriptome-wide ***,gene ontology(GO)classification and metabolic pathway annotation revealed eight targets that were actively involved in developmental *** the predicted miRNA-mRNA pairs,six targets were then randomly selected and experimentally validated by dual luciferase reporter assay,where three pairs were proved with potential targeting ***,to search for conserved miRNAs potentially involved in early development of ***,we combined in silico and experimental methods,which can be applied in other organisms as *** data implied important roles of miRNAs in the early embryonic development and also suggested the presence of complex miRNA-mRNA functional networks in ***.

关键词： microRNAs(miRNAs) identification Marsupenaeus japonicus embryo transcriptome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computational identification and characterization of microRNAs and their targets in Penaeus monodon

引用

Journal of Oceanology and Limnology 2018年第3期36卷 853-869页

作者：濮龙军王晶王玉左建伟郭华荣 Ministry of Education Key Laboratory of Marine Genetics and Breeding College of Marine Life Sciences Ocean University of China Institute of Evolution and Marine Biodiversity Ocean University of China

Study on shrimp miRNAs was limited and just 7 mature miRNA sequences of Marsupenaeus japonicus are deposited in mir Base database. In this study, miRNAs and their target gene candidates were computationally identified from shrimp Penaeu s monodon and then experimentally validated. Using 39 908 expressed sequence tags(ESTs) and 21 124 genome survey sequences(GSSs) of P. monodon(pmo) as reference dataset, a comprehensive approach based on inter-species homolog search was employed to investigate the candidate miRNAs(i.e. pmo-miRNA). A total of eight miRNAs belonging to 7 families were computationally identified and five out of them were subsequently validated by PCR and sequencing. Of these, pmo-miR-4961a, pmo-miR-4961b, pmo-miR-4979 and pmo-miR-3819 were first identified from shrimps. Both the mature pmo-miRNAs and the corresponding precursors were conserved among different species. Based on perfect or near-perfect match to the target region, the target gene candidates of pmomiRNAs were predicted from 10 331 mRNA sequences of P. monodon. A total of 20 genes were predicted as the targets of pmo-miR-4961a, pmo-miR-4961b, pmo-miR-4979 and pmo-miR-6492. Experimental validation by dual luciferase reporter assay confi rmed the targeting between 3 pmo-miRNAs and one or two of their target genes, especially the pmo-miR-4979 which could significantly down-regulate the expression of target gene(JR226772). This study updates the miRNAs and their targets in P. monodon and lays a solid foundation for future RNAi study.

关键词：碱基数据对虾生态系统生物学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

机器人辅助全膝关节置换术的安全性及有效性研究

引用

中华骨科杂志 2023年第1期43卷 31-40页

作者：浦路桥叶涛舒隆钧郭英张金洪普光民唐江龙蒙旭晗唐志方卜鹏飞徐永清李川解放军联勤保障部队第九二〇医院骨科昆明650032 大理市第一人民医院骨科大理671099 昆明市中医医院骨科昆明650011 昭通市第二人民医院骨科昭通657000 楚雄彝族自治州中医医院骨科楚雄675000 迪庆藏族自治州人民医院骨科香格里拉674499

目的探讨机器人辅助全膝关节置换术(total knee arthroplasty,TKA)的安全性和有效性。方法2021年8月至2022年3月收集六家医院因膝关节炎性疾病接受机器人辅助TKA治疗的69例患者资料,男24例、女45例,年龄(52±6)岁(范围46~72岁),包括膝... 详细信息

目的探讨机器人辅助全膝关节置换术(total knee arthroplasty,TKA)的安全性和有效性。方法2021年8月至2022年3月收集六家医院因膝关节炎性疾病接受机器人辅助TKA治疗的69例患者资料,男24例、女45例,年龄(52±6)岁(范围46~72岁),包括膝关节骨关节炎53例、类风湿关节炎10例、创伤性关节炎6例。术前提取患者的CT数据并通过机器人系统设计个性化的假体摆位方案,确定假体尺寸、下肢力线及股骨和胫骨截骨量;术中根据假体摆位方案由机器人控制截骨模板完成截骨,测量术中截骨量与术前规划截骨量以验证机器人辅助TKA的截骨准确性。术后根据X线片测量冠状面的股骨远端外侧角、胫骨近端内侧角和髋-膝-踝角;术后3个月采用美国膝关节协会评分(Keen Society score,KSS),包括KSS膝评分和功能评分,评价术后疗效。结果69例患者手术时间为(97.3±2.3)min(范围80~110 min),出血量为(320.0±6.2)ml(范围300~350 ml)。膝关节活动度由术前的82.2°±1.1°提高至术后119.7°±0.8°,差异有统计学意义(t=27.65,P<0.001);下肢力线由术前的160.9°±0.5°改善至术后的178.0°±0.2°,差异有统计学意义(t=32.03,P<0.001)。KSS膝评分由术前的(54.8±0.7)分提高至术后3个月的(85.0±0.5)分,KSS功能评分由术前的(56.5±0.7)分提高至术后的(85.9±0.4)分,差异均有统计学意义(t=35.45、36.58,均P<0.001)。术中股骨与胫骨截骨量与术前规划相比截骨误差在2 mm以内的患者比例为:胫骨平台外侧97%,胫骨平台内侧100%,股骨远端外侧100%,股骨远端内侧99%,股骨后髁外侧93%,股骨后髁内侧100%;术后正位X线片测量角度误差在3°以内的患者比例为:股骨远端外侧角为100.0%,胫骨近端内侧角为100.0%,髋-膝-踝角为100%。除1例术后伤口发生脂肪液化外,所有患者均无其他并发症发生。结论采用机器人辅助TKA假体安装准确、术后下肢力线恢复良好,是一种安全、有效的治疗膝关节炎性疾病的手术方式。

关键词：关节炎关节成形术,置换,膝机器人手术

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A miniaturized,Omni-Directional UWB Biconical Antenna with Band-Notched

A miniaturized,Omni-Directional UWB Biconical Antenna with B...

引用

2011 4th IEEE International Symposium on Microwave,Antenna,Propagation and EMC Technologies for Wireless Communications

作者： longjun Zhang Fushun Zhang Zhenlin Liao pu Wan Gaoli Ning National Key Laboratory of Science and Technology on Antennas and Microwaves Xidian University Xi’an Shannxi 710071 China

A miniaturized,omni-directional Ultra Wideband （UWB） biconical antenna with band-notched is ***,we design an antenna which is the well-known biconical *** in order to improve the structure of the flat ground for miniaturization,the biconical antenna above a concavo-convex ground is designed without degrading the *** antenna operates from 2.4-20 GHz with low Voltage Standing Wave Ratio （VSWR＜2）.Finally,the miniaturized antenna operates from 2.4-20 GHz with low VSWR （＜2） too is designed,excluding a band- notched frequency at *** propose antennas are fed by a coaxial probe through a SMA *** length of the proposed biconical element above the ground plane is slightly less than λ/4 at the lowest frequency in the *** results for VSWR and radiation pattern of the four antennas are presented and discussed.

关键词： omni-directional UWB biconical antenna band-notched

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

晕动病发病中水通道蛋白1的变化

引用

中国耳鼻咽喉头颈外科 2013年第6期20卷 302-305页

作者：黄益灯夏思文吴龙军戴朴韩东一解放军第一一八医院耳鼻咽喉科浙江温州325000 解放军总医院耳鼻咽喉头颈外科北京100853

目的探讨小鼠晕动病发病过程中内耳水通道蛋白的变化。方法多次旋转刺激后检测小鼠内耳中一系列水通道蛋白的mRNA水平;通过相关性分析观察内耳中水通道蛋白1的mRNA水平与个体晕反应指数之间的关系。通过内耳注射包含有水通道蛋白1的RNA... 详细信息

目的探讨小鼠晕动病发病过程中内耳水通道蛋白的变化。方法多次旋转刺激后检测小鼠内耳中一系列水通道蛋白的mRNA水平;通过相关性分析观察内耳中水通道蛋白1的mRNA水平与个体晕反应指数之间的关系。通过内耳注射包含有水通道蛋白1的RNA干扰质粒的病毒,抑制内耳中水通道蛋白1的表达,观察其对小鼠晕反应指数的影响。结果多次旋转刺激后,小鼠的晕反应指数逐渐下降;小鼠内耳中水通道蛋白1的mRNA水平明显增加;通过RNA干扰的方法局部抑制小鼠内耳中水通道蛋白1的表达,发现会增加小鼠的晕反应指数。结论多次旋转刺激后,小鼠的晕反应指数的下降可能是缘于内耳中水通道蛋白1的表达增加。小鼠内耳中水通道蛋白1在晕动病的发生过程中起到重要作用。

关键词：晕动病小鼠水孔蛋白质类动物实验逆转录聚合酶链反应迷路

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：