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A Bifunctional-Blocker-Aided Hybridization Chain Reaction Lighting-Up Self-calibrating Nanocluster Fluorescence for Reliable Nucleic Acid Detection

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Journal of Analysis and Testing 2024年第2期8卷 160-169页

作者： Dan Zhang guobao Zhou Hongyan Yang Yi Wang Lijun Shen Yuxuan Qiu Lei Li longhua guo College of Chemistry and Materials Science Zhejiang Normal UniversityJinhua321004China Jiaxing Key Laboratory of Molecular Recognition and Sensing College of BiologicalChemical Sciences and EngineeringJiaxing UniversityJiaxing314001China College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou314000China

In this work,we proposed a ratiometric silver nanoclusters(AgNCs)fluorescent assay by designing a bifunctional-blockeraided hybridization chain reaction(HCR).Hairpin probe 1(HP1)containing two special DNA fragments(5′-CAC CGC T-3′and 5′-ATT TGC CTT TTG GGG ACG GATA-3′)at two terminals creates a red-emitting AgNC nucleation sequence(rNS,5′-CAC CGC TAT TTG CCT TTT GGG GAC GGATA-3′).We found that the presence of a toehold fragment(5′-TGCCC-3′)in HP1 could silence the *** the addition of a target nucleic acid,HCR of HP1 and hairpin probe 2(HP2)could be initiated,resulting in the formation of long chain of DNA duplexes with multibranched *** the toehold fragment in HP1participated in generating duplexes,a strong emission of rNS-templated AgNCs was observed at 670 *** significantly,a bifunctional blocker was introduced not only to reduce the background red-emitting fluorescence but also to play as an internal green-emitting AgNCs nucleation *** the one hand,the blocker could increase the signal-to-noise-ratio of the constructed biosensor,and on the other hand,the blocker also helped to prepare ratiometric HCR-AgNCs assay with self-calibrating ability to strengthen its *** with the traditional HCR-AgNCs sensors,the developed ratiometric assay based on the bifunctional-blocker-aided HCR has higher reliability,which is important for the fabrication of biosensors in various fields for practical biosensing applications.

关键词： Ratiometric fluorescence Hybridization chain reaction Silver nanocluster Biosensor Bifunctional blocker

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基于金纳米球的肠炎沙门菌SERS免疫层析快速检测技术

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畜牧与兽医 2024年第5期56卷 90-98页

作者：陈中婷杨松鑫张福鑫蒙淑玲陈海兰郭隆华江明生王悦靓广西大学动物科学技术学院广西南宁530004 嘉兴学院生物与化学工程学院浙江嘉兴314001 广西壮族自治区兽用生物制品工程研究中心广西南宁530004 广西畜禽繁育与疾病防控重点实验室广西南宁530004 广西高校动物疫病预防与控制重点实验室广西南宁530004

为实现肠炎沙门菌的快速定量分析,采用Tris辅助柠檬酸钠还原法制备新型胶体金并利用Au-S配位作用在纳米金球表面修饰一层4-巯基苯甲酸(4-MBA)拉曼信号分子,然后以此为标记材料建立肠炎沙门菌表面增强拉曼(surface-enhancement of Raman ... 详细信息

为实现肠炎沙门菌的快速定量分析,采用Tris辅助柠檬酸钠还原法制备新型胶体金并利用Au-S配位作用在纳米金球表面修饰一层4-巯基苯甲酸(4-MBA)拉曼信号分子,然后以此为标记材料建立肠炎沙门菌表面增强拉曼(surface-enhancement of Raman scattering,SERS)免疫层析检测技术,并对其灵敏度、特异性和重复性进行考察。结果表明:建立的SERS免疫层析技术对肠炎沙门菌的可视化最低检测限(LOD)为10^(6) CFU/mL,而通过拉曼信号检测时LOD为10^(5) CFU/mL,灵敏度比裸眼观察提高了10倍。在10^(5)~10^(8) CFU/mL的浓度范围可根据标准曲线y=30.23×exp(-x/-147)-215.83(R^(2)=0.995)对肠炎沙门菌进行定量分析。该SERS免疫层析技术与鼠伤寒沙门菌、鸡白痢沙门菌、甲型副伤寒沙门菌等多种血清型沙门菌和大肠杆菌、副溶血弧菌等常见病原无交叉反应,特异性良好。同一批次的不同试纸条和不同批次的试纸条对相同样品的检测结果基本一致,重复性良好。以粪便和鸡蛋壳为基质的加标试验回收率在94.7%~104.9%之间,回收率良好。结果表明,基于金纳米颗粒的肠炎沙门菌SERS免疫层析快速检测技术灵敏度较高,特异性强,重复性好,能弥补传统胶体金试纸条无法定量的不足,可用于肠炎沙门菌的快速定量检测。

关键词：肠炎沙门菌免疫层析胶体金表面增强拉曼散射快速检测

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Event-Oriented Dynamic Security Service for Demand Response in Smart Grid Employing Mobile Networks

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China Communications 2015年第12期12卷 63-75页

作者： guo longhua DONG Mianxiong Kaoru Ota WU Jun LI Jianhua School of Electronic Information and Electrical Engineering Shanghai Jiao Tong University Department of Information and ElectronicEngineering Muroran Institute of Technology

Equipped with millions of sensors and smart meters in smart gird,a reliable and resilient wireless communication technology is badly *** networks are among the major energy communication networks which contribute to global energy consumption increase *** one of core technologies of smart grid employing mobile networks,Demand Response(DR) helps improving efficiency,reliability and security for electric power grid *** of DR events is one of the most important issues in ***,the security requirements of different DR events are dynamic for variousactual *** address this,an event-oriented dynamic security service mechanism is proposed for *** kinds of security services including security access service,security communication service and security analysis service for DR event are composited dynamically by the fine-grained sub *** experiment prototype of the network of State Grid Corporation of China(SGCC) is *** and evaluations shows the feasibility and effectiveness of the proposed scheme in smart grid employing mobile network.

关键词：国家电网公司安全服务智能电表需求响应事件无线通信技术移动网络通信网络

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梅州地区387例恶性淋巴瘤临床病理特点分析

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上海医药 2022年第9期43卷 34-36,46页

作者：叶敏郭珑华赖莉芬梅州市人民医院肿瘤内科梅州514000

目的:分析梅州地区387例恶性淋巴瘤(ML)患者的病理类型及临床特点。方法:收集我院收治的387例恶性淋巴瘤患者资料,从性别、发病年龄、病理类型、原发部位等方面进行回顾性分析。结果:霍奇金淋巴瘤(HL)40例(10.4%),非霍奇金淋巴瘤(NHL)34... 详细信息

目的:分析梅州地区387例恶性淋巴瘤(ML)患者的病理类型及临床特点。方法:收集我院收治的387例恶性淋巴瘤患者资料,从性别、发病年龄、病理类型、原发部位等方面进行回顾性分析。结果:霍奇金淋巴瘤(HL)40例(10.4%),非霍奇金淋巴瘤(NHL)347例(89.6%)。男女性别比例为1.58∶1,中位年龄57岁。NHL中最常见的类型为弥漫大B细胞淋巴瘤(56.8%);HL中90%为金典型霍奇金淋巴瘤,年龄分布未见双峰表现。HL和NHL发病部位均以颈部淋巴瘤多见。分期Ⅲ/Ⅳ期占58.2%,晚期转移部位主要为骨髓侵犯(27.4%)。结论:387例ML患者中男性比例较高,弥漫大B细胞淋巴瘤最为常见,晚期单发骨髓侵犯多见。

关键词：恶性淋巴瘤病理类型发病特点转移部位

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有机-无机混杂纤维改性氧化铝基陶瓷型芯孔隙-强度协同强化机制

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硅酸盐学报 2024年第1期52卷 180-188页

作者：芦刚罗龙华陈晓严青松于航陈义斯郭永光南昌航空大学轻合金加工科学与技术国防重点学科实验室南昌330063

为解决高孔隙铝基陶瓷型芯强度不足的问题,向陶瓷浆料中混杂不同比例的短切尼龙有机纤维(Nsf)与短切无机氧化铝纤维(Asf),采用热压注成型法制备高孔隙高强度氧化铝基陶瓷型芯。结果表明:Nsf氧化烧失显著增加型芯气孔率。Asf通过脱黏、... 详细信息

为解决高孔隙铝基陶瓷型芯强度不足的问题,向陶瓷浆料中混杂不同比例的短切尼龙有机纤维(Nsf)与短切无机氧化铝纤维(Asf),采用热压注成型法制备高孔隙高强度氧化铝基陶瓷型芯。结果表明:Nsf氧化烧失显著增加型芯气孔率。Asf通过脱黏、拔出、断裂等机制有效消耗裂纹扩展能,提高型芯抗弯强度。当Nsf/Asf质量分数(w)比为6:7(w(Nsf)=0.90%,w(Asf)=1.05%)时,氧化铝基陶瓷型芯具备最佳的综合性能,当其抗弯强度为20.1 MPa,显气孔率达到43.31%,线收缩仅为0.2%,可满足精铸需求。因此,调配有机-无机纤维比例,能有效增加型芯孔隙率还可以弥补纤维氧化烧失导致的强度损失,显著降低了型芯收缩,保证型芯尺寸精度和烧结成型率。

关键词：氧化铝陶瓷型芯纤维增强多孔陶瓷

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不同机动方式对机载武器系统传递对准性能影响研究

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弹箭与制导学报 2006年第1期26卷 1-4页

作者：郭隆华王新龙北京航空航天大学宇航学院北京100083

建立了“速度+姿态角”匹配下传递对准滤波器模型。详细研究了不同机动方式对机载武器捷联惯导系统传递对准性能的影响,并对计算机仿真结果进行了深入的分析。该研究成果为实现机载武器动基座快速精确初始对准技术在工程中的应用提供了... 详细信息

建立了“速度+姿态角”匹配下传递对准滤波器模型。详细研究了不同机动方式对机载武器捷联惯导系统传递对准性能的影响,并对计算机仿真结果进行了深入的分析。该研究成果为实现机载武器动基座快速精确初始对准技术在工程中的应用提供了理论依据。

关键词：惯导系统卡尔曼滤波传递对准机动方式

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干旱区棉花灌溉定额及灌溉制度实验分析

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陕西水利 2019年第6期 94-95,98页

作者：何玉敬郭龙华昌吉水文勘测局

新疆地处干旱内陆区,大力发展节水农业是保障水资源可持续利用的根本途径。为合理开发水资源,推进农业节水灌溉提高用水效率。对新疆巴音郭楞蒙古自治州棉花作物进行灌溉试验,提出干旱区棉花种植合理的灌溉定额和灌溉制度,为提高农业用... 详细信息

新疆地处干旱内陆区,大力发展节水农业是保障水资源可持续利用的根本途径。为合理开发水资源,推进农业节水灌溉提高用水效率。对新疆巴音郭楞蒙古自治州棉花作物进行灌溉试验,提出干旱区棉花种植合理的灌溉定额和灌溉制度,为提高农业用水效率、合理配置水资源和保障最严格水资源管理制度实施提供基础支撑。

关键词：干旱区节水灌溉灌溉定额用水效率

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Differences and Correlations of Morphological and Hemodynamic Parameters between Anterior Circulation Bifurcation and Side-wall Aneurysms

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Current Medical Science 2024年第2期44卷 391-398页

作者： Kai-kai guo Chang-ya LIU Gao-hui LI Jian-ping XIANG Xiao-chang LENG Yi-ke CAI Xue-bin HU Department of Neurosurgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Emergency Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Artery Flow Technology Co. Ltd.Hangzhou 310051China

Objective:The objective of this research was to explore the difference and correlation of the morphological and hemodynamic features between sidewall and bifurcation aneurysms in anterior circulation arteries,utilizing computational fluid dynamics as a tool for ***:In line with the designated inclusion criteria,this study covered 160 aneurysms identified in 131 patients who received treatment at Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,China,from January 2021 to September *** follow-up digital subtraction angiography(DSA)data,these cases were classified into two distinct groups:the sidewall aneurysm group and the bifurcation aneurysm *** and hemodynamic parameters in the immediate preoperative period were meticulously calculated and examined in both groups using a three-dimensional DSA reconstruction ***:No significant differences were found in the morphological or hemodynamic parameters of bifurcation aneurysms at varied locations within the anterior ***,pronounced differences were identified between sidewall and bifurcation aneurysms in terms of morphological parameters such as the diameter of the parent vessel(Dvessel),inflow angle(θF),and size ratio(SR),as well as the hemodynamic parameter of inflow concentration index(ICI)(P0.5,P<0.001).Conclusions:The significant differences in certain morphological and hemodynamic parameters between sidewall and bifurcation aneurysms emphasize the importance to contemplate variances in threshold values for these parameters when evaluating the risk of rupture in anterior circulation *** it is a bifurcation or sidewall aneurysm,these disparities should be *** morphological parameter SR has the potential to be a val

关键词： sidewall bifurcation unruptured aneurysms computational fluid dynamics

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Chinese Medicine Prolongs Overall Survival of Chinese Patients with Advanced Gastric Cancer: Treatment Pattern and Survival Analysis of a 20-Year Real-World Study

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Chinese Journal of Integrative Medicine 2024年第6期30卷 489-498页

作者： CAO Ni-da ZHU Xiao-hong MA Fang-qi XU Yan DONG Jia-huan QIN Meng-meng LIU Tian-shu ZHU Chun-chao guo Wei-jian DING Hong-hua guo Yuan-biao LIU Li-kun SONG Jin-jie WU Ji-ping CHENG Yue-lei ZENG Lin ZHAO Ai-guang Oncology Department I Longhua Hospital Shanghai University of Traditional Chinese MedicineShanghai200032China Oncology Department Zhongshan HospitalFudan UniversityShanghai200032China Gastrointestinal Surgery Department Renji HospitalShanghai Jiaotong University School of MedicineShanghai200127China Oncology Department Fudan University Shanghai Cancer CenterShanghai200032China Oncology Department Shanghai General HospitalShanghai Jiaotong University School of MedicineShanghai200080China Traditional Chinese Medicine Department Ruijin HospitalShanghai Jiaotong University School of MedicineShanghai200025China Oncology Department Shanxi Traditional Chinese Medicine HospitalTaiyuan030012China Oncology Department Xiyuan HospitalChina Academy of Chinese Medical ScienceBeijing102445China Oncology Department Yunnan Province Hospital of Traditional Chinese MedicineKunming650021China Oncology Department Shuguang HospitalShanghai University of Traditional Chinese MedicineShanghai201203China Shanghai NewCore Biotechnology Co. Ltd.Shanghai200240China

Objective To describe the treatment patterns and survival status of advanced gastric cancer(AGC)in China in the past two decades,and objectively evaluate the impact of standardized Chinese medicine(CM)treatment on the survival of AGC *** This multicenter registry designed and propensity score analysis study described the diagnosis characteristics,treatment-pattern development and survival status of AGC from 10 hospitals in China between January 1,2000 and July 31,*** survival(OS)was evaluated between non-CM cohort(standard medical treatment)and CM cohort(integrated standard CM treatment≥3 months).Propensity score matching(PSM)and inverse probability of treatment weighting(IPTW)were performed to adjust any difference in average outcomes for *** A total of 2,001 patients histologically confirmed locally advanced and/or metastasis stomach and gastroesophageal junction adenocarcinoma were *** them,1,607 received systemic chemotherapy,215(10.74%)accepted molecular targeted therapy,44(2.2%)received checkpoint inhibitor therapy,and 769(38.43%)received ***-drug regimen was the main choice for first-line treatment,with fluoropyrimidine plus platinum as the most common regimen(530 cases,60.09%).While 45.71%(16 cases)of patients with HER2 amplification received trastuzumab in *** application of apatinib increased(33.33%)in *** application of checkpoint inhibitors has increased since *** analysis showed that Lauren mixed type(P=0.017),cycles of first-line treatment>6(P=0.000),CM(P=0.000),palliative gastrectomy(P=0.000),trastuzumab(P=0.011),and apatinib(P=0.008)were independent prognostic factors for the OS of *** PSM and IPTW,the median OS of CM cohort and non-CM cohort was 18.17 and 12.45 months,respectively(P<0.001).Conclusions In real-world practice for AGC in China,therapy choices consisted with ***-drug regimen was the main first-line *** CM treatment was an independent

关键词： advancedgastriccancer real-world study treatment pattern survival analysis Chinese medicine

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Host cyclophilin A facilitates SARS-CoV-2 infection by binding and stabilizing spike on virions

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Signal Transduction and Targeted Therapy 2024年第1期9卷 36-39页

作者： Xiangpeng Sheng Fang Zhu Hong Peng Fan Yang Yi Yang Cong Yang Zhen Wang Wei Chen Deyin guo Ronggui Hu State Key Laboratory of Molecular Biology Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghaiChina MOE Key Laboratory of Tropical Disease Control Centre for Infection and Immunity StudySchool of MedicineSun Yat-sen UniversityShenzhenChina ICU Longhua Hospital Shanghai University of Traditional Chinese MedicineShanghaiChina

Dear Editor,The novel coronavirus SARS-CoV-2 rapidly evolutes to increase its infectivity and transmissibility,facilitates its immune escape,impairs vaccine efficacy,and currently causes repeated infections in human,which is mainly determined by the accumulated mutations in spike(S).1 Transmembrane S is a heavily glycosylated protein on the surface of the virion as homotrimer,responsible for binding to human angiotensin-converting enzyme 2(hACE2)receptor.2 While the architecture of S trimers has been well studied,how the stability of S is regulated by potential host factors remains unknown.

关键词： ACE2 vaccine infection

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