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境外宗教渗透进高校的四重审思

广东省社会主义学院学报 2024年第3期 36-42页

作者：张龙华张倩南京信息工程大学环境科学与工程学院江苏南京210044 南京信息工程大学管理工程学院江苏南京210044

境外宗教渗透进高校严重破坏我国高等教育事业的健康发展,但任何形式的境外宗教渗透进高校都离不开渗透主体、渗透客体和渗透介体这三个核心要素的相互作用。为了更好防范和抵御境外宗教渗透进高校,从谁来渗透、渗透给谁、如何渗透三个... 详细信息

境外宗教渗透进高校严重破坏我国高等教育事业的健康发展,但任何形式的境外宗教渗透进高校都离不开渗透主体、渗透客体和渗透介体这三个核心要素的相互作用。为了更好防范和抵御境外宗教渗透进高校,从谁来渗透、渗透给谁、如何渗透三个维度,对境外宗教渗透进高校的主体、客体、介体进行辩证审思;再从何以应对这一维度,审思高校反境外宗教渗透的对策。当前,境外宗教渗透进高校的主体主要为境外宗教组织和境外敌对势力等;客体主要包括高校大学生、专任教师和管理服务人员;介体画像多样,呈现三个特点。高校反境外宗教渗透的对策是构建对渗透主体的精准识别机制,加强对渗透客体的系统深入教育,完善对渗透介体的综合监管体系。

关键词：高校境外宗教渗透校园传教四重审思

M1DCNN模型在燃气调压器故障诊断的应用

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城市燃气 2024年第5期 9-14页

作者：王强李泽明张龙桦无锡华润燃气有限公司润智科技有限公司

燃气调压器的健康状况直接关系到燃气输配系统的稳定和安全运行,针对调压器的故障识别,需要大量的专家经验,且给燃气企业带来较大的运维成本。本文提出一种基于多尺度一维卷积神经网络(M1DCNN)的燃气调压器故障诊断模型,该模型在一维卷... 详细信息

燃气调压器的健康状况直接关系到燃气输配系统的稳定和安全运行,针对调压器的故障识别,需要大量的专家经验,且给燃气企业带来较大的运维成本。本文提出一种基于多尺度一维卷积神经网络(M1DCNN)的燃气调压器故障诊断模型,该模型在一维卷积神经网络的基础上,构建多个不同尺寸卷积核和池化层的通道,分别提取调压器出口压力数据特征信息并进行处理,最后进行特征融合,输出诊断故障类别。实验结果表明,M1DCNN模型能更全面的提取故障特征,有效进行故障识别,实现调压器智能故障诊断。

关键词：燃气调压器 CNN 多尺度特征提取故障诊断

Conformational change of *** sulfurtransferase YgaP upon SCN- in intact native membrane revealed by fluorescence lifetime and anisotropy

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Chinese Chemical Letters 2018年第10期29卷 1513-1516页

作者： Simeng Wang Yanan zhang longhua zhang Min zhang Changlin Tian School of Life Science Anhui University Hefei 230039 China National Laboratory of Physical Sciences at Microscale and School of Life Science University of Science and Technology of China Hefei 230027 China High Magnetic Field Laboratory Chinese Academy of Sciences Hefei 230031 China

Fluorescence lifetime and anisotropy has become a prevalent tool to detect the structure change and motility property of proteins. YgaP is the only membrane-integrated rhodanese in E. coli. The sulfur transfer process has been characterized by various studies. However, the mechanism of the outward transportation of SCN-remains unclear. In this work, we examined the fluorescence lifetime and anisotropy of site-specific incorporated unnatural amino acid 7-HC to study the conformational change of YgaP upon SCN-binding. We also compared the fluorescence changes between detergent-wrapped environment in DPC and intact native membrane environment in SMA. Our results suggested the presence of at least two different conformations in YgaP protein. Both the residues in the middle of TMH2 and the residues near extracellular side play important roles in the binding and/or output of SCN-. SMA is a good material to reflect the in situ conformation changes of protein than micelles.

关键词： Rhodanese YgaP Fluorescence lifetime and anisotropy SMA Conformation change

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn^2＋

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Chinese Chemical Letters 2018年第10期29卷 1509-1512页

作者： Mengge zhang Ming Wen Ying Xiong longhua zhang Changlin Tian National Laboratory of Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Hefei 230027 China High Magnetic Field Laboratory Chinese Academy of Sciences Hefei 230031 China

Fluorescence microscopy, as a sensitive method to detect microenvironment of molecules, is widely used in protein conformation and dynamic studies in live cells. Fluorescence lifetime imaging microscopy（FLIM）, which is independent of fluorophore concentrations, scattering and bleaching, is a suitable tool to analyze membrane proteins in a single cell. Ferroportin（FPN）, a multi-ion exporter in vertebrates, was modulated by metal ions with unknown mechanism. Herein, we fused green fluorescence protein on Cterminal of FPN（FPN-eGFP） and applied fluorescence lifetime to monitor conformation changes of FPN in a live cell. The fluorescence lifetime distribution showed a shift to shorter lifetime upon Mn^（2＋） treatment,suggesting a preference conformation of FPN in Mn^（2＋） exposure. It is also observed that the lifetime（rather than intensity） measurement was not strongly influenced by laser power. The observed fluorescence lifetime changes of FPN-eGFP upon Mn^（2＋） treatments indicated that extracellular metal ions can modulate FPN through conformation exchanges between several different states.

关键词： Fluorescence lifetime imaging microscopy In cell analysis of conformation change Ferroprotin

Fluorescence lifetime based distance measurement illustrates conformation changes of PYL10-CL2 upon ABA binding in solution state

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Chinese Chemical Letters 2019年第5期30卷 1067-1070页

作者： Peng Zhou Pei Lv Lu Yu Sanling Liu longhua zhang Changlin Tian High Magnetic Field Laboratory Chinese Academy of Sciences and School of Life Sciences University of Science and Technology of China

Forster resonance energy transfer(FRET)is a widely used distance measurement method to illustrate protein conformational *** FRET method relies on the distance between donor and acceptor,as well as the labelling efficiency,the size and the properties of the ***,we labelled a pair of small fluorophores and calculated the energy transferred efficiency through fluorescence lifetime analysis,which can provide more reliable distance measurement than intensity *** donor fluorophore,7-hydroxycoumarin-4-yl-ethylglycine(HC),was genetically incorporated into specific sites of PYL10,obtaining complete labelling *** acceptor fluorophore,Alexa488,was labelled through the disulfide bond,whose labelling efficiency was estimated through both absorption peaks and lifetime *** lifetime and anisotropy analysis showed ABA-induced local conformation changes and dynamics of several HC incorporation sites of *** lifetime-based FRET distance measurement illustrated the conformation changes of PYL10 with or without ABA application,which is consistent with the previously reported crystal structures.

关键词： Fluorescence lifetime Anisotropy Unnatural amino acid PYL10 Distance measurement

Metabolic state oscillations in cerebral nuclei detected using two-photon fluorescence lifetime imaging microscopy

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Chinese Chemical Letters 2023年第1期34卷 300-303页

作者： Peng Zhou Jiawei Shen Jun Liang Tian Xue Yuansheng Sun longhua zhang Changlin Tian High Magnetic Field Laboratory Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei 230031China The First Affiliated Hospital of USTC Division of Life Sciences and Medicineand Center for BioAnalytical ChemistryUniversity of Science and Technology of ChinaHefei 230026China ISS Inc.ChampaignIL 61822United States

The fluorescence lifetime of nicotinamide adenine dinucleotide(NADH),a key endogenous coenzyme and metabolic biomarker,can reflect the metabolic state of *** implement metabolic imaging of brain tissue at high resolution,we assembled a two-photon fluorescence lifetime imaging microscopy(FLIM)platform and verified the feasibility and stability of NADH-based two-photon FLIM in paraformaldehydefixed mouse cerebral ***,NADH based metabolic state oscillation was observed in cerebral nuclei suprachiasmatic nucleus(SCN).The free NADH fraction displayed a relatively lower level in the daytime than at the onset of night,and an ultradian oscillation at night was *** the combination of high-resolution imaging and immunostaining data,the metabolic tendency of different cell types was detected after the first two hours of the day and at ***,two-photon FLIM analysis of NADH in paraformaldehyde-fixed cerebral slices provides a high-resolution and label-free method to explore the metabolic state of deep brain regions.

关键词： NADH Fluorescence lifetime imaging microscopy Brain metabolism Metabolic oscillation High resolution

“双一流”背景下新医科实验教学课程思政探索与实践——以人体机能学实验课程为例

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高教学刊 2024年第22期10卷 31-34,39页

作者：汪铭陈聚涛张隆华倪芳胡兵宋怡胡媛萍中国科学技术大学生命科学与医学部合肥230027 中国科学技术大学马克思主义学院合肥230027

构建“双一流”大学的核心目的是培养一流人才,而课程思政是实现立德树人和“三全育人”的重要手段。新医科以培养“医学+X”复合型高层次医学人才为重点任务,人体机能学实验是基础医学实验的核心课程,是跨学科实验内容整合而成的独立学... 详细信息

构建“双一流”大学的核心目的是培养一流人才,而课程思政是实现立德树人和“三全育人”的重要手段。新医科以培养“医学+X”复合型高层次医学人才为重点任务,人体机能学实验是基础医学实验的核心课程,是跨学科实验内容整合而成的独立学科,知识点多元,思政元素丰富。该文以人体机能学实验教学为切入点,深挖思政元素,探讨新医科实验教学的课程思政建设目标、实施路径、教学案例和考核方式改进,以期将巩固理论知识、实验操作能力培养和正确价值观引领有机融合,全面提升课程质量,达到润物无声的效果。

关键词： “双一流”大学新医科课程思政实验教学人体机能学实验

Different conformational responses of the β2-adrenergic receptor-Gs complex upon binding of the partial agonist salbutamol or the full agonist isoprenaline

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National Science Review 2021年第9期8卷 111-120页

作者： Fan Yang Shenglong Ling Yingxin Zhou Yanan zhang Pei Lv Sanling Liu Wei Fang Wenjing Sun Liaoyuan A.Hu longhua zhang Pan Shi Changlin Tian Hefei National Laboratory of Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Amgen Asia R&D Center Amgen Research Shanghai 201210 China3High Magnetic Field Laboratory Chinese Academy of Sciences

G protein-coupled receptors(GPCRs) are responsible for most cytoplasmic signaling in response to extracellular ligands with different efficacy profiles. Various spectroscopic techniques have identified that agonists exhibiting varying efficacies can selectively stabilize a specific conformation of the ***, the structural basis for activation of the GPCR-G protein complex by ligands with different efficacies is incompletely understood. To better understand the structural basis underlying the mechanisms by which ligands with varying efficacies differentially regulate the conformations of receptors and G proteins, we determined the structures of β2AR-Gαsβγ bound with partial agonist salbutamol or bound with full agonist isoprenaline using single-particle cryo-electron microscopy at resolutions of 3.26 ?A and3.80 ?A, respectively. Structural comparisons between the β2AR-Gs-salbutamol and β2AR-Gs-isoprenaline complexes demonstrated that the decreased binding affinity and efficacy of salbutamol compared with those of isoprenaline might be attributed to weakened hydrogen bonding interactions, attenuated hydrophobic interactions in the orthosteric binding pocket and different conformational changes in the rotamer toggle switch in TM6. Moreover, the observed stronger interactions between the intracellular loop 2 or 3(ICL2 or ICL3) of β2AR and Gαswith binding of salbutamol versus isoprenaline might decrease phosphorylation in the salbutamol-activated β2AR-Gs complex. From the observed structural differences between these complexes of β2AR, a mechanism of β2AR activation by partial and full agonists is proposed to provide structural insights into β2AR desensitization.

关键词： cryo-EM structure G protein-coupled receptor (GPCR) partial and full agonists conformational change desensitization

Protein-protein interaction analysis in crude bacterial lysates using combinational method of ^19F site-specific incorporation and ^19F NMR

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Protein & Cell 2017年第2期8卷 149-154页

作者： Dong Li Yanan zhang Yao He Chengwei zhang Jiefei Wang Ying Xiong longhua zhang Yangzhong Liu Pan Shi Changlin Tian Hefei National Laboratory for Physical Science at the Microscale School of Life Science University of Science and Technology of China Hefei 230026 Anhui China School of Chemistry University of Science and Technology of China Hefei 230026 Anhui China

Protein-protein interactions （PPI） are essential for a variety of cellular functions. Many PPI analyses were conducted in vitro, using purified proteins. In this report, the unnatural amino acid tfmF was site-specifically incorporated into several different sites of two Phox-Beml （PB1） domains from two mitogen activated protein kinases （MEKK3 and/ or MEK5） in the E. coil cells.

关键词： Nuclear Magnetic Resonance Chemical Shift Change Nuclear Magnetic Resonance Method Crude Lysate Crude Cell Lysate