检索结果-南通市图书馆

Attenuation of ethylene signaling increases cotton resistance to a defoliating strain of Verticillium dahliae

作物学报：英文版 2023年第1期11卷 89-98页

作者： Tianyi Wang Muhammad Shaban Junhui Shi Weiran Wang Shiming Liu Xinhui Nie Yu Yu Jie Kong Steven J.Klosterman Xianlong zhang Alifu Aierxi longfu Zhu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Department of Plant Breeding and Genetics University of Agricu Institute of Economic Crops Xinjiang Academy of Agricultural SciencesKuqa 842000XinjiangChina Key Laboratory of Oasis Eco-agriculture of the Xinjiang Production and Construction Crops College of AgronomyShihezi UniversityShihezi 832000XinjiangChina Xinjiang Academy of Agricultural&Reclamation Sciences Shihezi 832000XinjiangChina United States Department of Agriculture Agricultural Research ServiceSalinasCA 93905USA

The severity of Verticillium wilt on cotton caused by defoliating strains of Verticillium dahliae has gradually increased and threatens production worldwide. Identification of the molecular components of leaf defoliation may increase cotton tolerance to V. dahliae. Ethylene, a major player in plant physiological processes, is often associated with senescence and defoliation of plants. We investigated the cotton–*** interaction with a focus on the role of ethylene in defoliation and defense against V. *** plants inoculated with V. dahliae isolate V991, a defoliating strain, accumulated more ethylene and showed increased disease symptoms than those inoculated with a non-defoliating strain. In cotton with a transiently silenced ethylene synthesis gene(GhACOs) and signaling gene(GhEINs) during cotton–V. dahliae interaction, ethylene produced was derived from cotton and more ethylene increased cotton susceptibility and defoliation rate. Overexpression of AtCTR1, a negative regulator in ethylene signaling, in cotton reduced sensitivity to ethylene and increased plant resistance to V. ***, the results indicated precise regulation of ethylene synthesis or signaling pathways improve cotton resistant to Verticillium wilt.

关键词： Cotton Verticillium dahilae Ethylene Defoliation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

High-temperature stress suppresses allene oxide cyclase 2 and causes male sterility in cotton by disrupting jasmonic acid signaling

引用

The Crop Journal 2023年第1期11卷 33-45页

作者： Aamir Hamid Khan Yizan Ma Yuanlong Wu Adnan Akbar Muhammad Shaban Abid Ullah Jinwu Deng Abdul Saboor Khan Huabin Chi longfu Zhu Xianlong zhang Ling Min National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Department of Botany University of MalakandDir Lower 18800Khyber PakhtunkwaPakistan

Cotton(Gossypium spp.) yield is reduced by stress. In this study, high temperature(HT) suppressed the expression of the jasmonic acid(JA) biosynthesis gene allene oxide cyclase 2(GhAOC2), reducing JA content and causing male sterility in the cotton HT-sensitive line H05. Anther sterility was reversed by exogenous application of methyl jasmonate(MeJA) to early buds. To elucidate the role of GhAOC2 in JA biosynthesis and identify its putative contribution to the anther response to HT, we created gene knockout cotton plants using the CRISPR/Cas9 system. Ghaoc2 mutant lines showed male-sterile flowers with reduced JA content in the anthers at the tetrad stage(TS), tapetum degradation stage(TDS), and anther dehiscence stage(ADS). Exogenous application of MeJA to early mutant buds(containing TS or TDS anthers) rescued the sterile pollen and indehiscent anther phenotypes, while ROS signals were reduced in ADS anthers. We propose that HT downregulates the expression of GhAOC2 in anthers, reducing JA biosynthesis and causing excessive ROS accumulation in anthers, leading to male sterility. These findings suggest exogenous JA application as a strategy for increasing male fertility in cotton under HT.

关键词： Cotton(Gossypium hirsutum) Jasmonic acid Allene oxide cyclase 2 ROS CRISPR/Cas9 High-temperature stress

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Histone H3 lysine 27 trimethylation suppresses jasmonate biosynthesis and signaling to affect male fertility under high temperature in cotton

引用

Plant Communications 2023年第6期4卷 96-112页

作者： Yanlong Li Miao Chen Aamir Hamid Khan Yizan Ma Xin He Jing Yang Rui zhang Huanhuan Ma Chunyang Zuo Yawei Li Jie Kong Maojun Wang longfu Zhu Xianlong zhang Ling Min National Key Laboratory of Crop Genetic Improvement&Hubei Hongshan Laboratory Huazhong Agricultural UniversityWuhan 430070HubeiChina Institute of Economic Crops Xinjiang Academy of Agricultural SciencesUrumqi 830091XinjiangChina

High-temperature(HT)stress causes male sterility in crops,thus decreasing *** explore the possible contribution of histone modifications to male fertility under HT conditions,we defined the histone methylation landscape for the marks histone H3 lysine 27 trimethylation(H3K27me3)and histone H3 lysine 4 trimethylation(H3K4me3)by chromatin immunoprecipitation sequencing(ChIP-seq)in two differing upland cotton(Gossypium hirsutum)*** observed a global disruption in H3K4me3 and H3K27me3 modifications,especially H3K27me3,in cotton anthers subjected to *** affected the bivalent H3K4me3–H3K27me3 modification more than either monovalent *** determined that removal of H3K27me3 at the promoters of jasmonate-related genes increased their expression,maintaining male fertility under HT in the HT-tolerant variety at the anther dehiscence *** jasmonate homeostasis or signaling resulted in an anther indehiscence phenotype under *** suppression of H3K27me3 deposition increased jasmonic acid contents and maintained male fertility under *** summary,our study provides new insights into the regulation of male fertility by histone modifications under HT and suggests a potential strategy for improving cotton HT tolerance.

关键词： high temperature H3K27me3 H3K4me3 cotton anther jasmonate

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

爬行器在页岩气水平井射孔中的应用研究

引用

油气藏评价与开发 2020年第5期10卷 77-83页

作者：王俊方张龙富夏海帮王玉海中国石化华东油气分公司江苏南京210019

为解决连续油管传输射孔周期长、费用高、长水平段自锁等问题,开展了爬行器射孔的攻关试验。目前页岩气水平井分段压裂主要采用泵送桥塞射孔联作进行分段压裂,首段射孔,因缺乏泵送通道,常采用连续油管传输射孔,作业周期相对较长,费用相... 详细信息

为解决连续油管传输射孔周期长、费用高、长水平段自锁等问题,开展了爬行器射孔的攻关试验。目前页岩气水平井分段压裂主要采用泵送桥塞射孔联作进行分段压裂,首段射孔,因缺乏泵送通道,常采用连续油管传输射孔,作业周期相对较长,费用相对较高,不利于降本增效开发。南川页岩气田通过引进研制改进部分工具,扩展了滚轮爬行器的业务范围,实现了页岩气水平井爬行器射孔。爬行器射孔通过牵引器提供动力,将射孔枪输送到射孔位置,点火完成射孔。通过现场改进及试验,南川页岩气田解决了电压隔离保护短节、减震短节、转接头3个方面的问题,掌握了爬行器射孔的性能及其适用条件,为后续施工提供经验和依据。试验结果表明,爬行器射孔在井斜小于90°的页岩气井水平段首段射孔是可行的。爬行器射孔能满足页岩气水平井带压射孔作业需求,作业过程受井斜、井筒洁净度的影响较大,相比连续油管射孔而言,其提速降本效果明显,单井节省施工周期2 d,提效达50%。

关键词：爬行器首段射孔页岩气水平井压裂

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Cytochrome P450 mono-oxygenase CYP703A2 plays a central role in sporopollenin formation and ms5ms6 fertility in cotton

引用

Journal of Integrative Plant Biology 2022年第10期64卷 2009-2025页

作者： Huanhuan Ma Yuanlong Wu Ruiling Lv Huabin Chi Yunlong Zhao Yanlong Li Hongbo Liu Yizan Ma longfu Zhu Xiaoping Guo Jie Kong Jianyong Wu Chaozhu Xing Xianlong zhang Ling Min National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China College of Biology and Agricultural Resources Huanggang Normal UniversityHuanggang 438000China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Institute of Economic Crops Xinjiang Academy of Agricultural SciencesXinjiang 830091China State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000China

The double-recessive genic male-sterile(ms)line ms5 ms6 has been used to develop cotton(Gossypium hirsutum)hybrids for many years,but its molecular-genetic basis has remained ***,we identified the Ms5 and Ms6 loci through map-based cloning and confirmed their function in male sterility through CRISPR/Cas9 gene ***5 and Ms6 are highly expressed in stages 7–9 anthers and encode the cytochrome P450 mono-oxygenases CYP703A2-A and *** ms5 mutant carries a single-nucleotide C-to-T nonsense mutation leading to premature chain termination at amino acid 312(GhCYP703A2-A^(312aa)),and ms6 carries three nonsynonymous substitutions(D98E,E168K,and G198R)and a synonymous mutation(L11L).Enzyme assays showed that GhCYP703A2 proteins hydroxylate fatty acids,and the ms5(GhCYP703A2-A^(312aa))and ms6(GhCYP703A2-D^(D98E,E168K,G198R))mutant proteins have decreased enzyme *** and lipidomic analyses showed that in ms5 ms6 plants,C12–C18 free fatty acid and phospholipid levels are significantly elevated in stages 7–9 anthers,while stages 8–10 anthers lack sporopollenin fluorescence around the pollen,causing microspore degradation and male ***,our characterization uncovered functions of GhCYP703A2 in sporopollenin formation and fertility,providing guidance for creating male-sterile lines to facilitate hybrid cotton production and therefore exploit heterosis for improvement of cotton.

关键词： cotton cytochrome P450 mono-oxygenase doublerecessive genic male sterility sporopollenin formation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Nickel and indium core-shell co-catalysts loaded silicon nanowire arrays for efficient photoelectrocatalytic reduction of CO_(2) to formate

引用

Journal of Energy Chemistry 2021年第3期30卷 422-428页

作者： Wenchao Ma Mingcan Xie Shunji Xie longfu Wei Yichen Cai Qinghong zhang Ye Wang State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy MaterialsNational Engineering Laboratory for Green Chemical Productions of AlcoholsEthers and EstersCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005FujianChina

Developing an efficient artificial photosynthetic system for transforming carbon dioxide and storing solar energy in the form of chemical bonds is one of the greatest challenges in modern ***,the limited choice of catalysts with wide light absorption range,long-term stability and excellent selectivity for CO_(2) reduction makes the process ***,a core-shell-structured nonnoble-metal Ni@In co-catalyst loaded p-type silicon nanowire arrays(SiNWs)for efficient CO_(2) reduction to formate is *** formation rate and Faradaic efficiency of formate over the Ni@In/SiNWs catalyst reach 58μmol h^(-1) cm^(-2) and 87% under the irradiation of one simulated sunlight(AM 1.5 G,100 mW cm^(-2)),respectively,which are about 24 and 12 times those over the pristine *** enhanced photoelectrocatalytic performance for CO_(2) reduction is attributed to the rational combination of Ni capable of effectively extracting the photogenerated electrons and In responsible for the selective activation of CO_(2).

关键词： CO_(2)reduction Photoelectrocatalysis Core-shell-structured co-catalyst Silicon nanowire arrays

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

老年甲状腺结节性病变96例临床分析

引用

中国临床医学 2010年第1期17卷 84-85页

作者：杨宇红张龙富刘溯曹东华孙熙融上海市长宁区中心医院老年科上海200336

目的:分析老年人甲状腺结节性病变的特点。方法:回顾分析96例因非甲状腺疾病就诊的住院老年患者的临床资料,患者于入院后次日清晨抽空腹静脉血测游离三碘甲状腺原氨酸(free triiodothyronine,FT3)、游离甲状腺素(free thyroxine,FT4)及... 详细信息

目的:分析老年人甲状腺结节性病变的特点。方法:回顾分析96例因非甲状腺疾病就诊的住院老年患者的临床资料,患者于入院后次日清晨抽空腹静脉血测游离三碘甲状腺原氨酸(free triiodothyronine,FT3)、游离甲状腺素(free thyroxine,FT4)及促甲状腺素(thyroid-stimulating hormone,TSH)等指标,行甲状腺B超检查,并对手术患者进行随访。结果:(1)老年人甲状腺结节的患病率67.71%,其中男女患病率有显著差异;(2)在甲状腺结节患者中,慢性支气管炎及恶性肿瘤的患病率明显增加(P<0.05)。(3)TSH越高,骨密度(bone mineral density,BMP)越低,两者呈负相关。(4)11例甲状腺结节患者进行手术治疗,病理诊断为:甲状腺乳头状癌1例,腺瘤3例,结节性甲状腺肿7例,9例术后甲状腺过氧化物酶抗体(thyroid per-oxidase antibody,TPOAb)升高,均需长期应用左旋甲状腺素。结论:老年人甲状腺结节的患病率较高,女性患病率更高;甲状腺结节患者中慢性支气管炎及恶性肿瘤的患病率明显增加;TSH与骨密度呈负相关;老年人甲状腺结节绝大多数为良性,应以内科随访及治疗为主,手术则需谨慎。

关键词：甲状腺结节甲状腺功能慢性支气管炎恶性肿瘤骨密度

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Down regulation of cotton GbTRP1 leads to accumulation of anthranilates and confers resistance to Verticillium dahliae

引用

Journal of Cotton Research 2019年第3期2卷 162-173页

作者： MIAO Yuhuan ZHU longfu zhang Xianlong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina University of Chinese Medicine Wuhan 430065HubeiChina

Background:Verticillium wilt,caused by Verticillium dahliae,is called a "cancer" disease of *** discovery and identification of defense-related genes is essential for the breeding of Verticillium wilt-resistant *** previous research we identified some possible broad-spectrum resistance ***,we report a tryptophan synthesis-related gene GbTRP1 and its functional analysis in relation to the resistance of cotton to ***:Expression analysis shows that GbTRP1 is suppressed at 1 h and 6 h post *** infection,but activated at 12 h and 24 h,and the expression of GbTRP1 is highly induced by treatment with salicylic acid and jasmonic ***-cellular localization studies show that GbTRP1 is localized in the *** of GbTRP1 expression leads to lesion-mimic phenotypes and activates the immune response in cotton by showing enhanced resistance to *** and *** analysis shows that anthranilic compounds significantly accumulated in GbTRP1-silenced plants,and these metabolites can inhibit the growth of *** and *** in ***:Our results show that suppression of GbTRPI expression dramatically activates the immune response and increases resistance of cotton to *** and ***,possibly due to the accumulation of anthranilate *** study not only provides genetic resources for disease resistance breeding,but also may provide a basis for new chemical control methods for combatting of fungal disease in cotton.

关键词： Cotton GbTRP1 Verticillium dahliae Botrytis cinerea Anthranilate compounds

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Suppression of the homeobox gene HDTF_1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton

引用

Journal of Integrative Plant Biology 2016年第5期58卷 503-513页

作者： Wei Gao Lu Long Li Xu Keith Lindsey Xianlong zhang longfu Zhu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University State Key Laboratory of Cotton Biology Henan Key Laboratory of Plant Stress Biology School of Life Sciences Henan University Integrative Cell Biology Laboratory School of Biological and Biomedical Sciences University of Durham

Development of pathogen-resistant crops, such as fungus-resistant cotton, has significantly reduced chemical application and improved crop yield and quality. However, the mechanism of resistance to cotton pathogens such as Verticillium dahliae is still poorly understood. In this study, we characterized a cotton gene （HDTF1） that was isolated following transcriptome profiling during the resistance response of cotton to V. dahliae. HDTFI putatively encodes a homeodomain transcription factor, and its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea. To characterise the involvement of HDTF1 in the response to these pathogens, we used virusinduced gene silencing （VlGS） to generate HDTFl-silenced cotton. VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens. HDTF1 silencing resulted in activation of jasmonic acid （JA）-mediated signaling and JA accumulation. However, the silenced plants were not altered in the accumulation of salicylic acid （SA） or the expression of marker genes associated with SA signaling. These results suggest that HDTF1 is a negative regulator of the JA pathway, and resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling.

关键词： Botrytis cinerea cotton jasmonic acid Verticillium dahliae virus-induced gene silencing

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Survey on the Demand for Elderly Care Services of Community Residents in Beijing and Analysis of Influencing Factors

引用

Journal of Clinical and Nursing Research 2024年第4期8卷 79-87页

作者： Yue Zhai Xianguo Bi Weimin Liu Hong Wang Xinxin zhang Jing Chen Beijing Century Forum Hospital Capital Medical UniversityBeijing 100038China Beijing Municipal Health Commission Beijing 100053 China Beijing longfu Hospital Beijing 100010China Yang Song Township Community Health Service Centre Huairou DistrictBeijing 101499China

Objective: To investigate the current situation of the demand for geriatric care services of community residents in Beijing and analyze the influencing factors to provide a reference basis for meeting the demand for diversified and professional geriatric care services. Methods: A self-made questionnaire was used to randomly survey 1558 elderly individuals at community health service centers in 8 urban districts where elderly care centers were planned to be built. The influencing factors of the different characteristics of elderly care service needs from three aspects were analyzed using a dichotomous logistic regression model: predisposing, enabling, and, need factors. Results: 69.7% of the elderly required home care services, 22.8% wanted to get care services at elderly care centers, 15.9% wanted to get care services at nursing homes, 12.3% required community care services, and 7.4% didn’t know where to access care services. 68.5% of the elderly required care services for disabilities/semi-disabilities, 58.0% for dementia, 54.7% for common diseases, 34.9% for rehabilitation training, 33.0% for plumbing care, and 7.5% for hospice care. At the same time, there were urban- rural differences in the demand for elderly care services, with suburban elderly having a higher demand for care services than those living in urban areas (P < 0.05). The elderly’s demand for care services was mainly related to age, place of residence, and gender in the causative factors, mode of residence and physical condition among able factors, and mode of care services and care needs among need factors (P < 0.05). Conclusion: The demand for elderly care services was differentiated by factors including place of residence, age, and gender. It is crucial to accurately match the demand for elderly care services, innovate the mode of elderly care services, and improve the service quality to improve the elderly health service system.

关键词： Elderly care Service system Demand Population ageing Influencing factors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：