检索结果-南通市图书馆

Exploration of reaction rates of chlorine dioxide with tryptophan residue in oligopeptides and proteins

Journal of Environmental Sciences 2020年第7期32卷 129-136页

作者： Yuexian Ge Yu Lei Xin Lei Wenhui Gan longfei shu Xin Yang School of Environmental Science and Engineering Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation TechnologySun Yat-sen UniversityGuangzhou 510275China Graduate School at Shenzhen Tsinghua UniversityShenzhen 518055China

Chlorine dioxide(ClO2),an alternative disinfectant to chlorine,has a superior ability to inactivate microorganisms,in which protein damage has been considered as the main inactivation ***,the reactivity of ClO2 with amino acid residues in oligopeptides and proteins remains poorly *** this research,we studied the reaction rate constants of ClO2 with tryptophan residues in five heptapeptides and four proteins using stopped-flow or competition kinetic *** heptapeptide and protein contain only one tryptophan residue and the reactivity of tryptophan residue with ClO2 was lower than that of free tryptophan(3.88×10^4(mol/L)^-1 sec^-1 at pH 7.0).The neighboring amino acid residues affected the reaction rates through promoting inter-peptide aggregation,changing electron density,shifting pKa values or inducing electron transfer via redox reactions.A single amino acid residue difference in oligopeptides can make the reaction rate constants differ by over 60%(e.g.3.01×10^4(mol/L)^-1 sec^-1 for DDDWNDD and 1.85×10^4(mol/L)^-1 sec^-1 for DDDWDDD at pH 7.0(D:aspartic acid,W:tryptophan,N:asparagine)).The reaction rates of tryptophan-containing oligopeptides were also highly pH-dependent with higher reactivity for deprotonated tryptophan than the neutral *** residues in proteins spanned a 4-fold range reactivity toward ClO2(i.e.0.84×10^4(mol/L)^-1 sec^-1 for ribonuclease T1 and 3.21×10^4(mol/L)^-1 sec^-1 for melittin at pH 7.0)with accessibility to the oxidant as the determinating *** local environment surrounding the tryptophan residue in proteins can also accelerate the reaction rates by increasing the electron density of the indole ring of tryptophan or inhibit the reaction rates by inducing electron transfer *** results are of significance in advancing understanding of ClO2 oxidative reactions with proteins and microbial inactivation mechanisms.

关键词： Chlorine dioxide(ClO2) Tryptophan Protein Disinfection Water treatment

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Synergistic cytotoxicity of binary combinations of inorganic and organic disinfection byproducts assessed by real-time cell analysis

引用

Journal of Environmental Sciences 2022年第7期34卷 222-231页

作者： Mengge Fan longfei shu Xinran Zhang Miao Yu Yongting Du Junlang Qiu Xin Yang Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology School of Environmental Science and EngineeringSun Yat-sen UniversityGuangzhou 510006China

Chlorine, chlorine dioxide, and ozone are widely used as disinfectants in drinking water treatments. However, the combined use of different disinfectants can result in the formation of various organic and inorganic disinfection byproducts(DBPs). The toxic interactions, including synergism, addition, and antagonism, among the complex DBPs are still unclear. In this study, we established and verified a real-time cell analysis(RTCA) method for cytotoxicity measurement on Chinese hamster ovary(CHO) cell. Using this convenient and accurate method, we assessed the cytotoxicity of a series of binary combinations consisting of one of the 3 inorganic DBPs(chlorite, chlorate, and bromate) and one of the 32 regulated and emerging organic DBPs. The combination index(CI) of each combination was calculated and evaluated by isobolographic analysis to reflect the toxic interactions. The results confirmed the synergistic effect on cytotoxicity in the binary combinations consisting of chlorite and one of the 5 organic DBPs(2 iodinated DBPs(I-DBPs) and 3 brominated DBPs(Br-DBPs)), chlorate and one of the 4 organic DBPs(3 aromatic DBPs and dibromoacetonitrile), and bromate and one of the 3 organic DBPs(2 I-DBPs and dibromoacetic acid). The possible synergism mechanism of organic DBPs on the inorganic ones may be attributed to the influence of organic DBPs on cell membrane and cell antioxidant system. This study revealed the toxic interactions among organic and inorganic DBPs, and emphasized the latent adverse outcomes in the combined use of different disinfectants.

关键词： Disinfection byproducts(DBPs) Real-time cell analysis(RTCA) Synergistic cytotoxicity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Evaluation of different primers of the 18S rRNA gene to profile amoeba communities in environmental samples

引用

Water Biology and Security 2022年第3期1卷 56-66页

作者： Xiafei Zheng Zhili He Cheng Wang Qingyun Yan longfei shu Environmental Microbiomics Research Center School of Environmental Science and EngineeringSouthern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Sun Yat-sen UniversityGuangzhou510006China Ninghai Institute of Mariculture Breeding and Seed Industry Zhejiang Wanli UniversityNingbo315100China

Amoeboid protists,an assemblage of organisms belonging to different phylogenetic lineages,have drawn increasing attention due to their crucial ecological roles in various environments and their potential health ***,18S rRNA gene sequencing is widely applied for the detection of ***,it is not clear which is the best primer pair for 18S rRNA gene amplification in *** study compared the four most commonly used primer pairs for revealing the diversity,composition,core species,and community assembly processes of amoebae in water and *** found that the choice of primers artificially influences the detection of community composition of *** also found that short-read fragments may lead to mismatches in taxonomy and were not suitable for phylogenetic *** contrast,full-length primers could detect the highest number of amoeba lineages and annotate 80%of reads belonging to amoebae to known ***,full-length primers did not detect as many amoeba species as V4 ***,we showed that beta diversity and community assembly determination were largely unaffected by primer choice,but different primers could influence our interpretations of the ecological process underlying stochasticity and *** study indicates that full-length read sequencing and V4 region Illumina sequencing are suitable for profiling amoeba diversity in the environment.

关键词： Amoeba community Diversity Drinking water safety Metagenomics 18S rRNA gene Protist

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The effects of exercise interventions on brain-derived neurotrophic factor levels in children and adolescents:a meta-analysis

引用

Neural Regeneration Research 2025年第5期20卷 1513-1520页

作者： Xueyun Shao longfei He Yangyang Liu Physical Education School Shenzhen UniversityShenzhenGuangdong ProvinceChina Shenzhen Institute of Neuroscience ShenzhenGuangdong ProvinceChina

Brain-derived neurotrophic factor is a crucial neurotrophic factor that plays a significant role in brain health. Although the vast majority of meta-analyses have confirmed that exercise interventions can increase brain-derived neurotrophic factor levels in children and adolescents, the effects of specific types of exercise on brain-derived neurotrophic factor levels are still controversial. To address this issue, we used meta-analytic methods to quantitatively evaluate, analyze, and integrate relevant studies. Our goals were to formulate general conclusions regarding the use of exercise interventions, explore the physiological mechanisms by which exercise improves brain health and cognitive ability in children and adolescents, and provide a reliable foundation for follow-up research. We used the Pub Med, Web of Science, Science Direct, Springer, Wiley Online Library, Weipu, Wanfang, and China National Knowledge Infrastructure databases to search for randomized controlled trials examining the influences of exercise interventions on brain-derived neurotrophic factor levels in children and adolescents. The extracted data were analyzed using Review Manager 5.3. According to the inclusion criteria, we assessed randomized controlled trials in which the samples were mainly children and adolescents, and the outcome indicators were measured before and after the intervention. We excluded animal experiments, studies that lacked a control group, and those that did not report quantitative results. The mean difference(MD;before versus after intervention) was used to evaluate the effect of exercise on brain-derived neurotrophic factor levels in children and adolescents. Overall, 531 participants(60 children and 471 adolescents, 10.9–16.1 years) were included from 13 randomized controlled trials. Heterogeneity was evaluated using the Q statistic and I^(2) test provided by Review Manager software. The meta-analysis showed that there was no heterogeneity among the studies(P = 0.67,

关键词： adolescents brain-derived neurotrophic factor children exercise meta-analysis randomized controlled trials

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Environmental selection and evolutionary process jointly shape genomic and functional profiles of mangrove rhizosphere microbiomes

引用

mLife 2023年第3期2卷 253-266页

作者： Xiaoli Yu Qichao Tu Jihua Liu Yisheng Peng Cheng Wang Fanshu Xiao Yingli Lian Xueqin Yang Ruiwen Hu Huang Yu Lu Qian Daoming Wu Ziying He longfei shu Qiang He Yun Tian Faming Wang Shanquan Wang Bo Wu Zhijian Huang Jianguo He Qingyun Yan Zhili He State Key Laboratory for Biocontrol Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)School of Environmental Science and EngineeringEnvironmental Microbiomics Research CenterSun Yat‐sen UniversityGuangzhouChina Institute of Marine Science and Technology Shandong UniversityQingdaoChina College of Forestry&Landscape Architecture South China Agricultural UniversityGuangzhouChina Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) School of Marine ScienceSun Yat‐sen UniversityGuangzhouChina Department of Civil and Environmental Engineering The University of TennesseeKnoxvilleTennesseeUSA Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems School of Life SciencesXiamen UniversityXiamenChina Xiaoliang Research Station for Tropical Coastal Ecosystems and Key Laboratory of Vegetation Restoration and Management of Degraded Ecosystems South China Botanical GardenChinese Academy of SciencesGuangzhouChina School of Life Sciences Sun Yat‐sen UniversityGuangzhouChina

Mangrove reforestation with introduced species has been an important strategy to restore mangrove ecosystem ***,how such activities affect microbially driven methane(CH4),nitrogen(N),and sulfur(S)cycling of rhizosphere microbiomes remains *** understand the effect of environmental selection and the evolutionary process on microbially driven biogeochemical cycles in native and introduced mangrove rhizospheres,we analyzed key genomic and functional profiles of rhizosphere microbiomes from native and introduced mangrove species by metagenome sequencing *** with the native mangrove(Kandelia obovata,KO),the introduced mangrove(Sonneratia apetala,SA)rhizosphere microbiome had significantly(p<0.05)higher average genome size(AGS)(5.8 vs.5.5 Mb),average 16S ribosomal RNA gene copy number(3.5 vs.3.1),relative abundances of mobile genetic elements,and functional diversity in terms of the Shannon index(7.88 vs.7.84)but lower functional potentials involved in CH4 cycling(e.g.,mcrABCDG and pmoABC),N2 fixation(nifHDK),and inorganic S cycling(dsrAB,dsrC,dsrMKJOP,soxB,sqr,and fccAB).Similar results were also observed from the recovered Proteobacterial metagenome-assembled genomes with a higher AGS and distinct functions in the introduced mangrove ***,salinity and ammonium were identified as the main environmental drivers of functional profiles of mangrove rhizosphere microbiomes through deterministic *** study advances our understanding of microbially mediated biogeochemical cycling of CH_(4),N,and S in the mangrove rhizosphere and provides novel insights into the influence of environmental selection and evolutionary processes on ecosystem functions,which has important implications for future mangrove reforestation.

关键词： average genome size functional potential mangrove rhizosphere metagenome metagenome-assembled genome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

阿米巴-细菌互作:进化、生态与环境效应

引用

微生物学报 2020年第5期60卷 864-874页

作者：彭涛张斯仪王璐婷何祯珍舒龙飞中山大学环境科学与工程学院南方海洋科学与工程广东省实验室(珠海)环境微生物组学研究中心广东广州510006

阿米巴是原生动物的主要类群,是陆地和水生生态系统的关键组成部分。阿米巴与细菌之间有着密切而复杂的相互作用。一方面,阿米巴通过捕食直接影响细菌群落与多样性,增强细菌活性。另一方面,细菌也进化出抵抗捕食的机制来抵抗甚至感染阿... 详细信息

阿米巴是原生动物的主要类群,是陆地和水生生态系统的关键组成部分。阿米巴与细菌之间有着密切而复杂的相互作用。一方面,阿米巴通过捕食直接影响细菌群落与多样性,增强细菌活性。另一方面,细菌也进化出抵抗捕食的机制来抵抗甚至感染阿米巴,反向影响阿米巴的生长和多样性。近年来,阿米巴-细菌互作的研究开始受到了广泛关注。本文总结了阿米巴-细菌互作的进化历史、生态关系(捕食、偏利共生、寄生和互利共生)以及其对环境的潜在影响,旨在更好地理解阿米巴-细菌互作这一研究领域,为其他原生动物-微生物之间的研究提供新的思路,也为探究宿主-微生物互作的机制提供参考。

关键词：原生动物阿米巴细菌宿主捕食共生协同进化

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

机器人气囊抛光位置误差建模及补偿

引用

机械科学与技术 2023年第5期42卷 724-729页

作者：毛记祥倪磊向北平舒龙飞西南科技大学制造过程测试技术教育部重点实验室四川绵阳621010

针对机器人气囊抛光中抛光压力波动的问题,分析影响抛光压力稳定的因素,提出误差补偿法来降低抛光压力波动对加工面形的影响。首先,分析气囊抛光的运动过程,根据Preston方程建立材料去除函数模型,使用MATLAB对去除函数进行仿真分析。然... 详细信息

针对机器人气囊抛光中抛光压力波动的问题,分析影响抛光压力稳定的因素,提出误差补偿法来降低抛光压力波动对加工面形的影响。首先,分析气囊抛光的运动过程,根据Preston方程建立材料去除函数模型,使用MATLAB对去除函数进行仿真分析。然后针对由机器人末端位置波动引入的系统误差,提出网格式误差补偿法,根据误差数据模型对加工点位进行即时修正,从而降低由机器人引入的抛光压力波动误差。实验数据表明:补偿后机器人末端位置X,Y方向误差波动值分别下降了86.2%,67.6%,机器人末端位置精度明显提高,最终加工面形精度的RMS为0.118λ。证明了该方法能够有效的减小抛光压力的波动,改善加工面形质量。

关键词：工业机器人气囊抛光去除函数误差补偿抛光压力

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

对侧入路在经皮微创通道减压术治疗小椎板退变型侧方腰椎椎管狭窄中的应用

引用

中华神经外科杂志 2023年第3期39卷 255-260页

作者：疏龙飞代飞虎张晨旭郑杰唐忠宇张亮王玉海赵伟解放军联勤保障部队第九〇四医院神经外科无锡214044

目的探讨对侧入路在经皮微创通道减压术治疗小椎板退变型侧方腰椎椎管狭窄中的应用价值。方法回顾性分析2019年9月至2020年12月解放军联勤保障部队第九〇四医院神经外科采用显微镜经皮微创通道对侧椎旁入路减压术治疗的15例小椎板退变... 详细信息

目的探讨对侧入路在经皮微创通道减压术治疗小椎板退变型侧方腰椎椎管狭窄中的应用价值。方法回顾性分析2019年9月至2020年12月解放军联勤保障部队第九〇四医院神经外科采用显微镜经皮微创通道对侧椎旁入路减压术治疗的15例小椎板退变型侧方腰椎椎管狭窄症患者的临床资料。比较CT上病变节段椎弓根上切迹切面术后椎板开窗宽度与棘突根部至关节突背侧关节面的距离,并评估术后双侧关节突关节面的磨除情况;观察MRI上术后硬膜囊横径、矢状径较术前的变化情况。术后定期行临床随访,评估疼痛视觉模拟量表(VAS)评分、日本骨科学会(JOA)评分和Oswestry功能障碍指数(ODI)较术前的改善情况。通过影像学随访评估腰椎的稳定性。结果15例患者均未发生与手术相关的神经损伤、脑脊液漏及颅内感染。手术时间为(74.9±13.6)min(60~100 min),术中出血量为(25.9±10.1)ml(15~50 ml)。术后CT显示症状对侧椎板开窗宽度为(9.0±1.2)mm,小于棘突根部至背侧关节面的距离[(13.1±1.2)mm](P<0.05);术后症状侧关节面的剩余宽度[(16.8±1.8)mm]大于术前关节面宽度的2/3[(12.4±1.3)mm],对侧关节面的剩余宽度[(15.6±1.7)mm]亦大于术前关节面宽度的2/3[(12.5±1.2)mm](均P<0.001)。硬膜囊的横径由术前的(7.8±1.5)mm增大至(14.9±1.2)mm,矢状径由术前的(9.2±1.8)mm增大至(14.8±2.1)mm,差异均有统计学意义(均P<0.001)。15例患者均获得随访,随访时间为(8.1±2.3)个月(6~12个月)。至末次随访,VAS评分由术前的(5.3±1.5)分下降至(0.6±0.2)分,JOA评分由术前的(14.5±1.8)分恢复至(26.7±5.3)分,ODI由术前的69.5%±13.8%减少至12.9%±2.1%,差异均有统计学意义(均P<0.05)。术后X线片检查未见腰椎失稳症或侧弯畸形。结论显微镜经皮微创通道对侧椎旁入路减压术治疗小椎板退变型侧方腰椎椎管狭窄症的疗效确切,减压充分,同时能够最大程度保护双侧的关节突关节,且不影响术后腰椎的稳定性和活动度。

关键词：腰椎椎管狭窄显微外科手术微通道对侧入路

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

黄韧带整体显露技术在经皮微创通道减压术治疗中央型腰椎管狭窄症中的应用

引用

中华神经外科杂志 2021年第10期37卷 1008-1013页

作者：疏龙飞代飞虎张旭程明张旺杨硕刘国东王玉海赵伟解放军联勤保障部队第九〇四医院神经外科无锡214044

目的探讨黄韧带整体显露技术在经皮微创通道单侧椎旁入路双侧减压术治疗中央型腰椎管狭窄症中的作用。方法回顾性分析解放军联勤保障部队第九〇四医院神经外科2019年3月至2020年6月采用经皮微创通道单侧椎旁入路双侧减压术治疗的中央型... 详细信息

目的探讨黄韧带整体显露技术在经皮微创通道单侧椎旁入路双侧减压术治疗中央型腰椎管狭窄症中的作用。方法回顾性分析解放军联勤保障部队第九〇四医院神经外科2019年3月至2020年6月采用经皮微创通道单侧椎旁入路双侧减压术治疗的中央型腰椎管狭窄症患者的临床资料,共24例。患者术中均采用黄韧带整体显露技术,观察术后关节突关节及硬膜囊的横径和矢状径的改变,评估术后1 d、7 d、1个月、3个月、6个月的日本骨科学会(JOA)评分、疼痛视觉模拟评分(VAS)及Oswestry功能障碍指数(ODI)较术前的改善情况。结果24例患者均未发生与手术相关的神经损伤、脑脊液漏及感染。腰椎单节段的手术时间为(72.0±16.1)min。术后CT显示关节突关节保护良好;MRI显示黄韧带切除完全,硬膜囊横径由术前的(9.4±2.6)mm增大至(16.0±3.0)mm,硬膜囊矢状径由术前的(10.2±1.9)mm增大至(15.1±1.9)mm,差异均有统计学意义(均P<0.05)。术后6个月随访显示,JOA由术前的(14.8±3.6)分恢复至(26.9±1.3)分,VAS评分由术前的(7.1±1.5)分减少至(0.5±0.2)分,ODI由术前的(68.4±11.8)%减少至(12.7±7.5)%,差异均有统计学意义(均P<0.05)。结论显微镜下经皮微创通道单侧椎旁入路双侧减压术中应用黄韧带整体显露技术治疗中央型腰椎管狭窄症疗效确切、椎管减压充分、关节突关节损伤小、不影响术后腰椎稳定性和活动度。

关键词：腰椎椎管狭窄显微外科手术黄韧带整体显露通道双侧减压

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

重型颅脑创伤患者开颅术后远隔部位再次手术的相关因素分析

引用

中华神经外科杂志 2017年第7期33卷 682-686页

作者：疏龙飞蔡桑赵伟朱晓明李培培张锋王玉海解放军第一〇一医院神经外科全军颅脑损伤救治中心无锡214044

目的探讨重型颅脑创伤（TBI）患者开颅术后远隔部位再次手术的相关危险因素。方法回顾性分析2010年2月至2014年10月解放军第一。一医院神经外科收治的180例行开颅手术的重型TBI患者的临床资料，其中39例（21．7％）患者远隔部位行二次... 详细信息

目的探讨重型颅脑创伤（TBI）患者开颅术后远隔部位再次手术的相关危险因素。方法回顾性分析2010年2月至2014年10月解放军第一。一医院神经外科收治的180例行开颅手术的重型TBI患者的临床资料，其中39例（21．7％）患者远隔部位行二次手术。分析术前格拉斯哥昏迷评分、年龄、性别、着力部位（额颞部、顶部、枕部、多发）、着力部位与血肿位置（同侧、对侧、双侧）、远隔手术部位骨折、术前瞳孔（正常、单侧散大、双侧散大）、环池（清晰、模糊）、中线移位（无、≤5mm、〉5～10mm、≥10mm）、术前缺氧、合并伤、是否放置脑室外引流、术后去骨瓣减压等因素对远隔部位再次手术的影响。结果多因素Logistic回归分析显示，额颞部着力同时血肿位于着力部位同侧是术后远隔部位再次手术的保护因素（OR=0．222，95％CI：0．073～0．674，P=0．008）；而枕部着力同时血肿位于对侧（OR=4．647，95％CI：1．078～20．033，P=0．039）、远隔手术部位骨折（OR=3．133，95％CI：1．247～7．867，P=0．015）、去骨瓣减压（OR=5．684，95％CI：1．759～18．370，P=0．004）是术后远隔部位再次手术的独立危险因素。结论对于重型TBI行开颅手术的患者，枕部着力且血肿位于对侧、手术远隔部位合并骨折、术后去骨瓣减压时，需密切观察病情变化，警惕远隔部位需再次手术。

关键词：颅脑损伤减压颅骨切除术再手术危险因素远隔部位

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：