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Flavonols Induced by 5-Aminolevulinic Acid Are Involved in Regulation of Stomatal Opening in Apple Leaves

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Horticultural Plant Journal 2016年第6期2卷 323-330页

作者： liu longbo XIONG Lijun AN Yuyan ZHENG Jie WANG Liangju College of Horticulture Nanjing Agricultural University

Flavonols, the main flavonoids in plant leaves, have newly been proposed as a H2O2 scavenger in guard cells. However, whether 5-aminolevulinic acid(5-ALA) is involved in regulating stomatal movement of apple(Malus × domestica Borkh. ‘Fuji') leaves through flavonol accumulation remains unclear. In this study, using diphenylboric acid 2-amino ethyl ester(DPBA, a flavonol fluorescent dye) and a laser scanning confocal microscope,we observed that 5-ALA pretreatment significantly improved flavonol accumulation in guard cells of apple leaves, especially around the *** we demonstrated that 5-ALA pretreatment inhibited ABA-induced stomatal closure via decreasing reaction oxygen species(ROS) accumulation in guard cells. Pretreatment with quercetin or kaempferol, two main kinds of flavonols in plants, also inhibited ABA-induced stomatal closure by decreasing ROS content in the guard cells. Furthermore, exogenous flavonols could suppress H2O2-induced stomatal closure in apple *** together, we conclude that ALA-induced flavonol accumulation in guard cells is involved in the inhibitory effect of ALA on ABA-induced ROS accumulation and stomatal closure in apple leaves.

关键词： apple(Malus × domestica Borkh.‘Fuji’) ABA 5-ALA flavonols stomatal aperture guard cell

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船载机电装备可靠性模拟试验技术综述

振动与冲击 2021年第17期40卷 167-174页

作者：张威刘隆波闻华殿张衍刘震涛浙江大学能源工程学院杭州310007 92942部队北京100161

高频振动载荷和低频摇摆载荷的综合作用是影响船载机电装备可靠性及寿命的主要因素。开展相应载荷下的船载机电装备可靠性模拟试验对装备的研发具有重要意义。调研并分析了多个领域在进行可靠性模拟试验时涉及的试验台架、控制算法及试... 详细信息

高频振动载荷和低频摇摆载荷的综合作用是影响船载机电装备可靠性及寿命的主要因素。开展相应载荷下的船载机电装备可靠性模拟试验对装备的研发具有重要意义。调研并分析了多个领域在进行可靠性模拟试验时涉及的试验台架、控制算法及试验方法。结果表明,现有的可靠性模拟试验只在单一振动载荷或者单一摇摆载荷下开展,不适用于模拟船载机电装备的真实工作环境。基于该研究现状,归纳整理出各个领域适用于船载机电装备的可靠性模拟试验技术,并在此基础上提出对船载机电装备今后更为合理的可靠性模拟试验的展望。

关键词：船载机电装备模拟试验振动载荷摇摆载荷耦合载荷

Monitoring of atmospheric radionuclides from the Fukushima nuclear accident and assessing their impact on Xi'an, China

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Chinese Science Bulletin 2013年第4期58卷 1585-1591页

作者： liu longbo WU Shan CAO JunJi XIE Feng SHI QuanLin ZHANG ChangYun TANG HanBing HE XiaoBin ZHANG Rong CHEN LiYun WEI GuanYi ZHANG ZhiHong ZHANG JiaMei DANG HaiJun Northwest Institute of Nuclear Technology Institute of Earth Environment Chinese Academy of Sciences

Aerosol radionuclides (131I, 134Cs, 137Cs) and gaseous radioactive xenon (133Xe) were monitored at Xi'an, China following the accident at the Fukushima nuclear power plant in March 2011. The additional annual effective dose attributable to the Fukushima emissions was much lower than the public annual effective dose from natural radiation, according to Chinese national standards. The monitoring results were compared with data from other countries as well as with the radionuclide concentrations observed in Xi'an after the Chernobyl nuclear accident in 1986. Possible transport pathways of the released radionuclides from Fukushima to Xi'an were investigated. The occurrence of an anticyclone in the Pacific Ocean region and the extended period over which the radionuclides were released made the determination transport pathways complex, but divergence in the plume and easterly flow evidently brought the initial suite of radionuclides to Xi'an.

关键词：切尔诺贝利核事故中国国家标准放射性核素监测结果西安年有效剂量评估大气

Development and validation of a nomogram prediction model for the risk of parastomal hernia

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Intelligent Medicine 2024年第2期4卷 128-133页

作者： Huasheng liu Weiqin Wang Chen Qin Hongxia Wang Wei Qi Yanhua Wei longbo Zheng Jilin Hu Department of Gastroenterology Affiliated Hospital of Qingdao UniversityQingdaoShandong 266003China Qingdao Municipal Hospital Group QingdaoShandong 266300China Qingdao Center Medical Group QingdaoShandong 266042China

Background Parastomal hernia is one of the potential complications after *** is currently no early risk assessment tool for parastomal *** The current investigation was conducted using retrospective studies.A total of 302 cases were used develop and internally to validate a nomogram prediction model,and 67 cases were used for external *** risk factors for parastomal hernia after permanent sigmoid colostomy were assessed via univariate analysis and binary logistic regression *** nomogram prediction model was established based on independent risk *** Body mass index,serum albumin,age,sex,and stoma diameter were independent risk factors for parastomal *** areas under the receiver operating characteristic curves were 0.909 in the development group and 0.801 in the validation *** Hosmer-Lemeshow test(P>0.05)and calibration curves indicated good consistency between actual observations and predicted *** A nomogram prediction model was constructed and validated based on risk factors for parastomal *** nomogram could be generalized to patients undergoing surgery for stoma by specialized surgeons to provide relevant references for stoma patients.

关键词： Nomogram Parastomal hernia Risk factors

石榴低温响应因子CBF基因家族鉴定及其表达分析

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西北农业学报 2022年第9期31卷 1154-1167页

作者：刘龙博郑树轩郑洁淮北师范大学生命科学学院安徽淮北253001 河南省农业广播电视学校夏邑分校河南商丘476400

CBF(C-repeat binding factor)是AP2家族的一类转录激活因子,在植物响应低温胁迫和提高植物耐寒性方面具有重要作用。虽然多个石榴品种的基因组已经发布,但仍缺乏对其CBF基因家族的全面研究。为全面分析石榴(Punica granatum L.)CBF基... 详细信息

CBF(C-repeat binding factor)是AP2家族的一类转录激活因子,在植物响应低温胁迫和提高植物耐寒性方面具有重要作用。虽然多个石榴品种的基因组已经发布,但仍缺乏对其CBF基因家族的全面研究。为全面分析石榴(Punica granatum L.)CBF基因家族分子生物学特性,本文对鉴定到的PgCBFs成员进行了生物信息和表达分析。结果表明,石榴全基因组中有7个CBF基因家族成员,蛋白序列中除包含AP2结构域外,还具有CBF特征序列PKKPAGRxKFxETRHP和DSAWR;7个成员集中分布在染色体1和4上,编码蛋白质氨基酸长度为201~267aa,分子质量为21.69~29.81ku;进化分析显示PgCBFs基因家族成员分布在GroupⅡ~Ⅳ亚组中,与水稻CBF成员组成的GroupⅠ存在明显区分;除PgCBF2、PgCBF4、PgCBF5基因结构中含有1~2个内含子外,其余与其他物种类似,属内含子缺失型;编码的蛋白序列均含有保守基序Motif1~7;蛋白二级结构主要为不规则卷曲和α-螺旋,三级结构较为相似。PgCBFs基因家族扩增主要来源于串联重复,并与拟南芥、苹果、桃分别存在2对、5对和4对共线性关系;GO注释显示PgCBFs多与转录调控、低温胁迫或激素诱导相关;启动子区含有多种顺势作用元件,主要为胁迫刺激、激素诱导和光响应元件;基因表达分析发现除PgCBF6外,其余成员在根中高度表达,在低温胁迫处理下PgCBF在根和韧皮部表达量显著上调,其中PgCBF7能够快速响应并持续应答低温诱导。结合进化树、共线性、启动子以及表达分析,推测PgCBF7可能与石榴幼苗低温胁迫调控相关。本研究可为深入研究石榴CBF基因家族生物学功能提供理论基础。

关键词：石榴(Punica granatum L.) CBF(C-repeat binding factor)基因家族生物信息学基因表达分析低温胁迫 PgCBF7

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凝并与沉降共存的直接蒙特卡罗模拟及其验证

计算机与应用化学 2012年第10期29卷 1237-1240页

作者：刘龙波张利兴西北核技术研究所陕西西安710024

气溶胶动力学方程是描述气溶胶动力学变化的主要方程,凝并与沉降共存问题是相关领域的常见的问题。建立了对该类问题的分段算法和直接蒙特卡罗模拟(Direct Simulation Monte Carlo method,DSMC)计算方法,对DSMC中事件后系统参数的更新... 详细信息

气溶胶动力学方程是描述气溶胶动力学变化的主要方程,凝并与沉降共存问题是相关领域的常见的问题。建立了对该类问题的分段算法和直接蒙特卡罗模拟(Direct Simulation Monte Carlo method,DSMC)计算方法,对DSMC中事件后系统参数的更新方法进行了改进,并提出了用纳入解析解的分段算法的结果对DSMC模拟结果进行验证的方法。结果表明,改进的DSMC算法显著提高了计算效率,模拟结果与其它算法的结果一致。

关键词：气溶胶动力学凝并沉降直接蒙特卡罗模拟通用动力学方程

Study on Sampling Technique of Atmospheric Radionuclide

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Meteorological and Environmental Research 2014年第6期5卷 1-3,8页

作者： Wu Shan liu longbo Zhang Changyun Tang Hanbing Chen Liyun Wu Yanmin Xie Feng Li Zhiming Shi Quanlin Dang Haijun Northwest Institute of Nuclear Technology

Atmospheric radionuclide monitoring usually includes two sampling techniques, namely ultra-high volume aerosol samplers to collect at- mospheric particles by using filter media, and radioactive noble gas samplers to collect atmospheric noble gas based on adsorption method. Atmos- pheric sampling techniques have been researched in Northwest Institute of Nuclear Technology since the Comprehensive Nuclear-Test-Ban Treaty （CTBT） was signed in 1996. Several ultra-high volume aerosol samplers and some types of radioactive xenon isotopes samplers had been devel- oped. For the aerosol sampler, the sampling flow is between 450 and 800 m3/h, with the minimum detectable concentration （MDC） of 131I less than 5 pBq/m3. For the xenon sampler, the sampling capacity of xenon is more than 4 ml per day, with MDC of l=Xe less than 0.25 mBq/m3. After the nuclear accident of Fukushima in 2011, monitoring of the atmospheric radionuclide was carried out for 3 months at Xi＇an, and part of radionuclide was detected with concentrations hiaher than their backorounds in the period, includina 131I.134Cs. 137Cs and 133Xe.

关键词： Atmospheric radionudide Sampling technique Fukushima nuclear accident Aerosol sampler Xenon sampler China

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浅谈环境影响评价与环境工程设计

山西化工 2019年第6期39卷 172-174页

作者：刘龙波山西绿航环境工程有限公司

随着近几年社会经济的迅速发展以及人们的生活水平的提高,人们对自身生存的环境有了更高的要求,当今社会所面临的最重要的问题就是环境保护。根据现在我国农业和工业生产的发展以及人口数量的不断增多,环境污染问题造成了生态失衡的现象... 详细信息

随着近几年社会经济的迅速发展以及人们的生活水平的提高,人们对自身生存的环境有了更高的要求,当今社会所面临的最重要的问题就是环境保护。根据现在我国农业和工业生产的发展以及人口数量的不断增多,环境污染问题造成了生态失衡的现象,直接威胁到了人们的生存。在环境管理工作中环境影响评价是一个重要的环节。本文主要分析了我国环境影响评价和环境工程设计,并找出了相应的解决措施进行深化探究。

关键词：环境影响评价环境工程设计环境管理

A pan-TE map highlights transposable elements underlying domestication and agronomic traits in Asian rice

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National Science Review 2024年第6期11卷 105-118页

作者： Xiaoxia Li Xiaofan Dai Huiying He Yang Lv longbo Yang Wenchuang He Congcong liu Hua Wei Xiangpei liu Qiaoling Yuan Xianmeng Wang Tianyi Wang Bintao Zhang Hong Zhang Wu Chen Yue Leng Xiaoman Yu Hongge Qian Bin Zhang Mingliang Guo Zhipeng Zhang Chuanlin Shi Qianqian Zhang Yan Cui Qiang Xu Xinglan Cao Dandan Chen Yongfeng Zhou Qian Qian Lianguang Shang Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural Sciences Rice Research Institute Shenyang Agricultural University Yazhouwan National Laboratory State Key Laboratory of Rice Biology China National Rice Research Institute National Key Laboratory of Tropical Crop Breeding Tropical Crops Genetic Resources InstituteChinese Academy of Tropical Agricultural Sciences

Transposable elements(TEs) are ubiquitous genomic components and hard to study due to being highly repetitive. Here we assembled 232 chromosome-level genomes based on long-read sequencing *** the 232 genomes with 15 existing assemblies, we developed a pan-TE map comprising both cultivated and wild Asian rice. We detected 177 084 high-quality TE variations and inferred their derived state using outgroups. We found TEs were one source of phenotypic variation during rice domestication and differentiation. We identified 1246 genes whose expression variation was associated with TEs but not single-nucleotide polymorphisms(SNPs), such as Os Rboh B, and validated Os Rboh B's relative expression activity using a dual-Luciferase(LUC) reporter assays system. Our pan-TE map allowed us to detect multiple novel loci associated with agronomic traits. Collectively, our findings highlight the contributions of TEs to domestication, differentiation and agronomic traits in rice, and there is massive potential for gene cloning and molecular breeding by the high-quality Asian pan-TE map we generated.

关键词： transposable element super pan-genome pan-TE rice