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The Electrochemical Behavior of Bleomycin at A Co/GC Ion Implantation Modified Electrode

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Chinese Chemical Letters 1999年第10期10卷 855-856页

作者： Jing Bo HU qi long li (Department of Chemistry, Beijing Normal University, Beijing 100875) Beijing Normal Univ Dept Chem Beijing 100875 Peoples R China

In 0.10 mol/L HOAc-NaOAc buffer solution (pH 4.59). a sensitive reduction peak of bleomycin is obtained by linear sweep voltammetry at Co/GC ion implantation modified electrode. Its electrochemical behavior has been studied. The experiments of AES and XPS show that Co is surely implanted into the surface of GCE and improved the electrocatalytic activity.

关键词： ion implantation electrochemical behavior bleomycin

Electrochemical Behavior of Adriamycin at Ni/GC Ion Implantation Modified Electrode

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Chinese Chemical Letters 2000年第7期11卷 601-602页

作者： Jing Bo HU qi long li(Department of Chemistry, Beijing Normal University, Bejing 100875) Beijing Normal Univ Dept Chem Beijing 100875 Peoples R China

With a Ni/GC ion implantation modified electrode as working electrode, in 0.1 mol/L HOAc-NaOAc (pH=4.62) solution, a sensitive reductive wave of ADM was obtained by linear sweep voltammetry. The peak potential was -0.55 V (***). The peak current is proportional to the concentration of ADM with a detection limit of 6.9 X 10(-8) mol/L. The behavior of the reduction wave was studied. The experiments of AES and XPS showed that Ni was surely implanted into the surface of the GCE and the implanted Ni at the GCE improved the electrocatalytic activity.

关键词： ion implantation modified electrode electrochemical behavior adriamycin

Electrochemical Studies of Paclitaxel Interaction with Tubulin

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Chinese Chemical Letters 2000年第4期11卷 351-352页

作者： Yong YU qi long li (Department of Chemistry, Beijing Normal Normal University. Beijing 100873) Beijing Normal Univ Dept Chem Beijing 100875 Peoples R China

A highly sensitive linear sweep voltammetric method was developed for the determination of paclitaxel and the mechanism of the binding of paclitaxel to tubulin was studied. Tubulin dimer formed with paclitaxel an electrochemically nonactive complex with a combination ratio of 2:2. Its stability constant was 2.85 x 10(22). So the tubulin dimer had two binding sites for paclitaxel. The experiment showed that the binding sites of paclitaxel to tubulin dimer were different from that of Ca2+ to tubulin dimer.

关键词： paclitaxel tubulin linear sweep voltammetry

Nucleases in gene-editing technologies:past and prologue

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National Science Open 2023年第5期2卷 25-56页

作者： Dan-Yuan li long-qi li Jun-Jie Gogo liu Beijing Advanced Innovation Center for Structural Biology Frontier Research Center for Biological StructureSchool of Life SciencesTsinghua UniversityBeijing 100084China Tsinghua-Peking Center for life Sciences School of Life SciencesTsinghua UniversityBeijing 100084China

In recent decades,gene-editing technologies,typically based on deoxyribonucleases to specifically modify genomic sequences,have dramatically remodeled various aspects of life sciences,including fundamental research,breeding,and medical *** far,four types of endonucleases have been adopted and optimized as gene-editing tools:meganuclease,ZFN,TALEN,and Cas nuclease from the CRISPR-Cas *** tool comes with its own advantages and *** the last ten years,RNA-guided Cas nucleases have been extensively investigated and successfully implemented in almost all mammalian cells due to their remarkable editing efficacy,high specificity,and flexibility in targeting the specific *** Cas nuclease,together with meganuclease,ZFN,and TALEN,represent the key strategies for nuclease-based gene ***,systematic introductions and comparisons among four types of nucleases are not yet ***,we overview the capabilities of four types of nucleases along the development history of gene editing and describe the molecular mechanisms of substrate recognition and ***,we summarize the promising CRISPR-Cas systems as well as modified tools applied for gene editing in the eukaryotic ***,how the re-modulated nucleases and other nucleases,either naturally occurring or AI-designed,might manipulate DNA sequences is discussed and proposed.

关键词： gene editing CRISPR ZFN TALEN meganuclease novel gene-editing system

Association of CASP9,CASP10 gene polymorphisms and tea drinking with colorectal cancer risk in the Han Chinese population

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第1期14卷 47-57页

作者： He liU Ming-wu ZHANG Yi-feng PAN Yun-xian YU Shan-chun ZHANG Xin-yuan MA qi-long li Kun CHEN Department of Epidemiology and Health Statistics School of Medicine Zhejiang University Hangzhou 310058 China Institute of Cancer Research and Prevention of Jiashan County Jiashan 314100 China

The initiators caspase-9 （CASP9） and caspase-10 （CASPIO） are two key controllers of apoptosis and play important roles in carcinogenesis. This study aims to explore the association between CASPs gene polymorphisms and colorectal cancer （CRC） susceptibility in a population-based study. A two-stage designed population-based case-control study was carried out, including a testing set with 300 cases and 296 controls and a validation set with 206 cases and 845 controls. A total of eight tag selected single nucleotide polymorphisms （SNPs） in CASP9 and CASPIO were chosen based on HapMap and the National Center of Biotechnology Information （NCBI） datasets and genotyped by restriction fragment length polymorphism （RFLP） assay. Multivariate logistic regression models were applied to evaluate the association of SNPs with CRC risk. In the first stage, from eight tag SNPs, three polymorphisms rs4646077 （odds ratio （OR）AA＋AG： 0.654, 95% confidence interval （CI）： 0.406-1.055; P=0.082）, rs4233532 （ORcc： 1.667, 95% CI： 0.967-2.876; Oacm： 1.435, 95% CI： 0.998-2.063; P=0.077）, and rs2881930 （ORcc： 0.263, 95% CI： 0.095-0.728, P=0.036） showed possible association with CRC risk. However, none of the three SNPs, rs4646077 （ORAA＋AG： 1.233, 95% CI： 0.903-1.683）, rs4233532 （ORcc： 0.892, 95% CI： 0.640-1.243; ORcT： 1.134, 95% CI： 0.897-1.433）, and rs2881930 （ORcc： 1.096, 95% CI： 0.620-1.938; ORcT： 1.009, 95% CI： 0.801-1.271）, remained significant with CRC risk in the validation set, even after stratification for different tumor locations （colon or rectum）. In addition, never tea drinking was associated with a significantly increased risk of CRC in testing set together with validation set （OR： 1.755, 95% CI： 1.319-2.334）. Our results found that polymorphisms of CASP9 and CASPIO genes may not contribute to CRC risk in Chinese population and thereby the large-scale case-control studies might be in consideration. In addition, tea drinking was a protective factor for CRC.

关键词： CASP9 CASPIO Colorectal cancer Single nucleotide polymorphisms Susceptibility to cancer Tea drinking

STUDIES ON VOLTAMMETRIC BEHAVIOUR OF 2-ANTIEPILEPSIRINE

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Chinese Chemical Letters 1993年第4期4卷 359-360页

作者： qi long li Cui ling MA Xin Yu liU Jun SHANG Department of Chemistry,Beijing Normal University,Beijing,100875

In a supporting electrolyte containing 0.6mol/L H_2SO_4,a sensitivity derivative wave of 2-AES is formed by *** peak potential is-1.01V(***),The change of peak height with the concentration of 2-AES is linear in the range from 3.0×10^(-7) to 8.0×10^(-6)mol/*** detection limit is 1.0×10^(-7)mol/*** electrode reaction mechanism is discussed.

关键词： SCE STUDIES ON VOLTAMMETRIC BEHAVIOUR OF 2-ANTIEPILEPSIRINE AES li

Mg-MOF-74对CO_2/H_2O的吸附性能

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高等学校化学学报 2017年第2期38卷 225-230页

作者：杜涛龙渊汤琦李生璐刘丽影东北大学国家环境保护生态工业重点实验室沈阳110819

以Mg(NO_3)_2·6H_2O和2,5-二羟基对苯二甲酸为原料,采用溶剂热法制备了金属有机骨架材料Mg-MOF-74.利用X射线衍射(XRD)、红外光谱(FTIR)和扫描电子显微镜(SEM)等测试手段对其结构、形貌和性能进行了分析,并利用自制穿透实验装置研究了... 详细信息

以Mg(NO_3)_2·6H_2O和2,5-二羟基对苯二甲酸为原料,采用溶剂热法制备了金属有机骨架材料Mg-MOF-74.利用X射线衍射(XRD)、红外光谱(FTIR)和扫描电子显微镜(SEM)等测试手段对其结构、形貌和性能进行了分析,并利用自制穿透实验装置研究了产物吸附CO_2/H_2O的性能.结果表明:合成的样品纯度高,结构完整,形貌规则有序,具有较高的CO_2吸附量.双组分CO_2/H_2O穿透实验结果证实,在水蒸气存在情况下,与沸石13X相比,Mg-MOF-74仍具有较高的CO_2吸附能力,可用于分离高湿烟道气中的CO_2.

关键词： Mg-MOF-74 穿透实验 CO2/H2O双组分吸附

Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin li Ting Zhang Yanlin Fu Peiyan long Xiao Gao Zhengwei Wang Zhizhong Guan Xiaolan qi Wei Hong Yan Xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

Bowel preparation before colonoscopy: Consequences, mechanisms, and treatment of intestinal dysbiosis

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World Journal of Gastroenterology 2025年第2期31卷 13-26页

作者： Ze-long Zheng qing-Fan Zheng li-qiang Wang Yi liu Department of Gastroenterology(Endoscopy Center) China-Japan Union Hospital of Jilin UniversityChangchun 130033Jilin ProvinceChina

The term“gut microbiota”primarily refers to the ecological community of various microorganisms in the gut,which constitutes the largest microbial community in the human *** adequate bowel preparation can improve the results of colonoscopy,it may interfere with the gut *** preparation for colonoscopy can lead to transient changes in the gut microbiota,potentially affecting an individual’s health,especially in vulnerable populations,such as patients with inflammatory bowel ***,measures such as oral pro-biotics may ameliorate these adverse *** focused on the bowel prepa-ration-induced changes in the gut microbiota and host health status,hypothesized the factors influencing these changes,and attempted to identify measures that may reduce dysbiosis,thereby providing more information for individualized bowel preparation for colonoscopy in the future.

关键词： Bowel preparation Colonoscopy Microbiota Inflammatory bowel disease Probiotics