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A conserved region in the 3' untranslated region of the human LIMK1 gene is critical for proper expression of LIMK1 at the post-transcriptional level

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Neuroscience Bulletin 2013年第3期29卷 348-354页

作者： Guang-fei Deng Shu-Jing Liu Xun-Sha Sun Wei-Wen Sun Qi-Hua Zhao Wei-Ping Liao Yong-Hong Yi Yue-Sheng long Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China Institute of Neuroscience and the Second Affiliated Hospital of Guangzhou Medical University Key Laboratory for Major Obstetric Diseases of Guangdong Province and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes Guangzhou Medical University

LIM kinase 1 （LIMK1）, a cytosolic serine/threonine kinase, regulates actin filament dynamics and reorganization and is involved in neuronal development and brain function. Abnormal expression of LIMK1 is associated with several neurological disorders. In this study, we performed a conservation analysis using Vector NTI （8.0） software. The dualluciferase reporter assay and real-time quantitative RT-PCR were used to assess the protein and mRNA levels of the reporter gene, respectively. We found that a region ranging from nt ＋884 to ＋966 in the human LIMK1 3＇ untranslated region （UTR） was highly conserved in the mouse Limkl 3＇ UTR and formed a structure containing several loops and stems. Luciferase assay showed that the relative luciferase activity of the mutated construct with the conserved region deleted, pGL4-hLIMK1-3U-M, in SH-SY5Y and HEK-293 cells was only -60% of that of the wild-type construct pGL4-hLIMK1-3U, indicating that the conserved region is critical for the reporter gene expression. Real-time quantitative RT-PCR analysis demonstrated that the relative Luc2 mRNA levels in SH-SY5Y and HEK293 cells transfected with pGL4-hLIMK1-3U-M decreased to50% of that in cells transfected with pGL4-hLIMK1- 3U, suggesting an important role of the conserved region in maintaining Luc2 mRNA stability. Our study suggests that the conserved region in the LIMK1 3＇ UTR is involved in regulating LIMK1 expression at the post-transcriptional level, which may help reveal the mechanism underlying the regulation of LIMK1 expression in the central nervous system and explore the relationship between the 3＇-UTR mutant and neuroloqical disorders.

关键词： LIM kinase 1 region post-transcriptsecondary structurethree-prime untranslatedonal regulation RNA

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飞行器陶瓷基复合材料轻量化结构设计研究进展

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航空材料学报 2024年第4期44卷 1-15页

作者：马宇张大海吴军荆瑞徐培飞费庆国东南大学机械工程学院南京 211189 东南大学高速飞行器结构与热防护教育部重点实验室南京 211189

高马赫数飞行带来的极端服役环境对新一代高速飞行器的材料、结构设计提出了更加严苛的要求,本文从"选、用、评"三方面对陶瓷基复合材料在飞行器结构设计中的应用进行综述,进而提出未来发展方向,为飞行器陶瓷基复合材料结构设计提供参考... 详细信息

高马赫数飞行带来的极端服役环境对新一代高速飞行器的材料、结构设计提出了更加严苛的要求,本文从"选、用、评"三方面对陶瓷基复合材料在飞行器结构设计中的应用进行综述,进而提出未来发展方向,为飞行器陶瓷基复合材料结构设计提供参考.全面综述了陶瓷基复合材料在不同应用场景下的选取准则及相应制备方法,系统介绍了陶瓷基复合材料在飞行器结构中的典型应用,分析了近服役工况下材料的评价准则及地面实验方法.为满足未来飞行器需求,提出需要结合计算机辅助优化技术和创新制备方法,提高陶瓷基复合材料的耐温和抗疲劳性能;发展高可靠、长寿命的连接技术和一体成型设计方案,充分发挥材料优势;开发多物理场耦合作用下的原位表征技术,以获得陶瓷基复合材料在实际使用中的性能演化行为,为飞行器轻量化结构设计提供可靠依据.

关键词：飞行器陶瓷基复合材料热防护结构轻量化结构

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Two-dimensional vanadium nanosheets as a remarkably effective catalyst for hydrogen storage in MgH_(2)

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Rare Metals 2021年第11期40卷 3195-3204页

作者： Zhi-Yu Lu Hai-Jie Yu Xiong Lu Meng-Chen Song Fu-Ying wu Jia-Guang Zheng Zhi-fei Yuan Liu-Ting Zhang School of Energy and Power Jiangsu University of Science and TechnologyZhenjiang 212003China Analysis and Testing Center Jiangsu University of Science and TechnologyZhenjiang 212003China

Magnesium hydride(MgH_(2)),which possesses high hydrogen density of 7.6 wt%,abundant resource and non-toxicity,has captured intense attention as one of the potential hydrogen storage ***,the practical application of Mg/MgH_(2) system is suffering from high thermal stability,sluggish absorption and desorption ***,two-dimensional(2D) vanadium nanosheets(V_(NS)) were successfully prepared via a facile wet chemical ball milling method and proved to be highly effective on improving the hydrogen storage performance of MgH_(2).For instance,the MgH_(2)+7 wt% V_(NS) composite began to release hydrogen at 187.2℃,152 ℃ lower than that of additive-free MgH_(2).At 300℃,6.3 wt% hydrogen was released from the MgH_(2)+7 wt% V_(NS) composite within 10 *** addition,the fully dehydrogenated sample could absorb hydrogen even at room temperature under hydrogen pressure of 3.2 MPa.X-ray diffractometer(XRD) and transmission electron microscopy(TEM)results confirmed metallic vanadium served as catalytic unit for facilitating the de/rehydrogenation reaction of MgH_(2).This finding presents an example of facile synthesis of two-dimensional(2D) vanadium with excellent catalysis,which may shed light on future design and preparation of highly effective layered catalysts for hydrogen storage and other energy-related areas.

关键词： Hydrogen storage Magnesium hydride Two-dimensional vanadium nanosheets Catalytic effect

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高地应力软岩隧道超前平行导洞开挖对主洞影响:以玉龙雪山隧道工程为例

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科学技术与工程 2024年第10期24卷 4265-4275页

作者：马建华尤刚王化武雷飞胡雯杰胡自强杨刚铭郝艳妲中国铁路昆明局集团有限公司滇西铁路建设指挥部大理671000 西南交通大学极端环境岩土和隧道工程智能建养全国重点实验室成都610031 中国铁路昆明局集团有限公司昆明650200 中铁隧道局集团建设有限公司北京100020

为研究高地应力软岩隧道超前平行导洞开挖对主洞影响,依托玉龙雪山隧道工程,基于现场长期监测数据,结合有限差分程序FLAC3D建立数值分析模型,研究超前平导对主洞围岩应力、围岩位移和塑性区分布的影响,探明主洞与平导间最优间距。研究... 详细信息

为研究高地应力软岩隧道超前平行导洞开挖对主洞影响,依托玉龙雪山隧道工程,基于现场长期监测数据,结合有限差分程序FLAC3D建立数值分析模型,研究超前平导对主洞围岩应力、围岩位移和塑性区分布的影响,探明主洞与平导间最优间距。研究结果表明:主洞开挖过程中,当掌子面超过监测面3.63倍主洞宽度的距离后,监测面拱顶沉降、上收敛、中收敛和下收敛值占最终变形值的80%以上,围岩变形稳定后上收敛值和中收敛值均大于拱顶沉降;平导超前开挖可有效改善主洞围岩应力环境,主洞与平导间距较大时,围岩应力改善效果不佳,随着二者间距逐渐减小,围岩应力改善效果逐渐增强,但主洞与平导间距过小时,二者开挖产生的塑性区会贯通,综合考虑,确定主洞与导洞最优间距为3.5倍导洞宽度;主洞拱顶沉降值和仰拱隆起值随着主洞与平导间距的减小而增大,左右拱腰水平位移值随着主洞与平导间距的减小先减小后增大。

关键词：应力释放最优间距超前平行导洞塑性区现场监测

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基于层次特征复用的视频超分辨率重建

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自动化学报 2024年第9期50卷 1736-1746页

作者：周圆王明非杜晓婷陈艳芳天津大学电气自动化与信息工程学院天津300072

当前的深度卷积神经网络方法,在视频超分辨率任务上实现的性能提升相对于图像超分辨率任务略低,部分原因是它们对层次结构特征中的某些关键帧间信息的利用不够充分.为此,提出一个称作层次特征复用网络(Hierarchical feature reuse netwo... 详细信息

当前的深度卷积神经网络方法,在视频超分辨率任务上实现的性能提升相对于图像超分辨率任务略低,部分原因是它们对层次结构特征中的某些关键帧间信息的利用不够充分.为此,提出一个称作层次特征复用网络(Hierarchical feature reuse network,HFRNet)的结构,用以解决上述问题.该网络保留运动补偿帧的低频内容,并采用密集层次特征块(Dense hierarchical feature block,DHFB)自适应地融合其内部每个残差块的特征,之后用长距离特征复用融合多个DHFB间的特征,从而促进高频细节信息的恢复.实验结果表明,提出的方法在定量和定性指标上均优于当前的方法.

关键词：层次特征复用卷积神经网络特征融合视频超分辨率重建

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Indica rice restorer lines with large sink potential exhibit improved nutrient transportation to the panicle,which enhances both yield and nitrogen-use efficiency

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Journal of Integrative Agriculture 2021年第6期20卷 1438-1456页

作者： TIAN Qing-lan HE Lian-hua LIAO Shuang LI wu DENG fei ZHOU Wei ZHONG Xiao-yuan REN Wan-jun Key Laboratory of Crop Ecophysiology and Farming System in Southwest China/Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province/College of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China Biotechnology Research Institute Guangxi Academy of Agricultural SciencesNanning 530007P.R.China

The yield and nitrogen use efficiency(NUE)of hybrid rice combinations are closely related to restorer ***,it is essential to evaluate the agronomic characteristics of restorer lines with high yield and high NUE(HYHN).However,it is unclear which restorer lines are HYHN,and neither have the common agronomic traits of the HYHN restorer lines been *** to address this issue,we conducted two filed experiments using three nitrogen applications,which screened five HYHN restorer lines from 15 indica restorer ***,NUE and nutrient transportation of restorer lines with different yields and NUE types were *** and total nitrogen absorption in aboveground biomass(TNA)increased,whereas NUE for grain production decreased with increasing nitrogen application *** HYHN restorer lines had large spikelets and high weight per panicle that were significantly positively correlated with yield and ***,large sink potential may be beneficial for both yield and *** further studied the differences in nutrient transportation to panicles between the HYHN and low yield and low NUE(LYLN)restorer lines and found that the former had a higher nitrogen absorption level and dry matter weight ratios of panicle in ***,the HYHN lines also had a higher root and neck-panicle node bleeding intensity per stem after heading and more developed vascular bundles of neck-panicle nodes and leaves than the LYLN lines,which could contribute to the transportation of nutrients from root to ground and from stem and leaf to ***,the advantages of large sink potential of the HYHN restorer lines include large nutrient accumulation in and distribution to the panicles and smooth flow of nutrients along the transportation channels.

关键词： indica hybrid rice restorer lines grain yield nitrogen-use efficiency sink potential nutrient transportation

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Regioregular Non-Fused Polymerized Small Molecular Acceptors Enabling Efficient All-Polymer Solar Cells

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Chinese Journal of Chemistry 2023年第7期41卷 790-796页

作者： Baoqi wu Youle Li Shizeng Tian Yue Zhang Langheng Pan Kangzhe Liu Mingqun Yang fei Huang Yong Cao Chunhui Duan Institute of Polymer Optoelectronic Materials and Devices State Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhouGuangdong510640 China

Comprehensive Summary The regioregularity induced by the isomers of the end-groups has been widely recognized as a key factor that determines the photovoltaic properties of polymerized small molecular acceptors(PSMAs)in all-polymer solar cells(all-PSCs).However,the influence of regioregularity on the photovoltaic properties of non-fused PSMAs has not been explored *** this contribution,two regioregular non-fused PSMAs,PFBTz-T-γand PFBTz-T-δ,were synthesized for the first time by using the monomers with isomeric pure *** with PFBTz-T-δ,PFBTz-T-γhas more compact and more ordered packing in solid state,which results in a more red-shifted optical absorption and a higher electron *** remarkably,PFBTz-T-γand PFBTz-T-δexhibited huge difference in photovoltaic performance in all-PSCs,which offered the power conversion efficiencies(PCEs)of 9.72%and 0.52%,*** studies have unveiled that the higher PCE of PFBTz-T-γis due to more efficient exciton dissociation,higher and more balanced electron/hole mobility,and less charge recombination as a result of favorable morphology of the blend *** work demonstrates that the development of regioregular non-fused PSMAs by tuning the polymerization sites is an effective strategy for obtaining high-efficiency all-PSCs.

关键词： All-polymer solar cells Non-fused framework Polymer acceptors Regioregularity Morphology regulation

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Acoustic Prediction and Risk Evaluation of Shallow Gas in Deep-Water Areas

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Journal of Ocean University of China 2022年第5期21卷 1147-1153页

作者： YANG Jin wu Shiguo TONG Gang WANG Huanhuan GUO Yongbin ZHANG Weiguo ZHAO Shaowei SONG Yu YIN Qishuai XU fei College of Safety and Ocean Engineering China University of Petroleum(BeijingBeijing 102249China Institute of Deep-Sea Science and Engineering Chinese Academy of SciencesSanya 572000China CNOOC Research Institute Beijing 100028China CNOOC China Limited Shenzhen Branch Shenzhen 518000China CNOOC China Limited Beijing 100010China

Shallow gas is a potential risk in deep-water drilling that must not be ignored,as it may cause major safety problems,such as well kicks and ***,the pre-drilling prediction of shallow gas is *** this reason,this paper conducted deep-water shallow gas acoustic simulation experiments based on the characteristics of deep-water shallow soil properties and the theory of sound wave speed *** results indicate that the propagation speed of sound waves in shallow gas increases with an in-crease in pressure and decreases with increasing *** and sound wave speed are basically functions of the power *** with the theory of sound wave propagation in a saturated medium,this paper establishes a multivariate functional relationship between sound wave speed and formation pressure and *** numerical simulation method is adopted to simulate shal-low gas eruptions under different pressure *** gas pressure coefficients that fall within the ranges of 1.0-1.1,1.1-1.2,and exceeding 1.2 are defined as low-,medium-,and high-risk,respectively,based on actual *** risk assessment me-thod has been successfully applied to more than 20 deep-water wells in the South China Sea,with a prediction accuracy of over 90%.

关键词： shallow gas acoustic simulation experiment sound wave speed pressure coefficient risk evaluation

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花椒味花生仁真空入味工艺优化及其品质分析

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食品工业科技 2024年第8期45卷 190-199页

作者：陈晓晴廖卢艳吴卫国湖南农业大学食品科学技术学院湖南长沙412000

花生传统加工方式存在能耗大、入味慢、加工周期长等缺点,为了得到风味更佳的花椒味花生仁休闲食品,本研究采用真空技术与传统技术相结合的加工方式,通过负压时间、负压次数、真空度三个单因素实验筛选真空入味工艺参数条件,以花椒酰胺... 详细信息

花生传统加工方式存在能耗大、入味慢、加工周期长等缺点,为了得到风味更佳的花椒味花生仁休闲食品,本研究采用真空技术与传统技术相结合的加工方式,通过负压时间、负压次数、真空度三个单因素实验筛选真空入味工艺参数条件,以花椒酰胺含量和感官评分为考察指标,在单因素实验的基础上通过响应面试验优化真空入味花椒味花生仁的工艺条件。结果表明,各因素对花椒味花生仁的影响顺序为负压时间>负压次数>真空度,最佳工艺参数为负压时间100 s、负压次数6次、真空参数0.80 MPa。该最佳工艺制作的花椒花生仁蛋白质含量为27%,脂肪含量为57%,过氧化值和酸价均符合国家的标准,感官评分为93.1分。本研究以推动标准化加工的坚果休闲食品全产业链提供参考,促进坚果休闲食品产业的可持续发展。

关键词：花生仁加工方式响应面优化品质分析

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生长分化因子5与代谢性疾病

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生物化学与生物物理进展 2024年第3期51卷 564-574页

作者：王天幕任无竞田振军陕西师范大学体育学院暨运动生物学研究所西安710119

生长分化因子5(growth/differentiation factor-5,GDF-5)属于转化生长因子β(transforming growth factor-β,TGF-β)家族,在骨、软骨、心脏、大脑、肾脏、骨骼肌和肌腱、肝脏以及脂肪等多个器官组织中表达。GDF-5与其受体BMPR-I/BMPR-II结合,激活Smad1/5/8、PI3K/Akt、p38-MAPK等信号,发挥促进细胞增殖分化、减少氧化应激损伤、细胞凋亡和组织纤维化等生物学功能。目前针对GDF-5的研究多聚焦在骨、软骨与肌腱的生长和修复等方面,而在其他器官中的生物学作用鲜有报道。因此,本文通过梳理和总结近年来GDF-5与代谢性疾病的研究进展,为GDF-5在改善代谢性疾病防治提供新的见解和理论依据。

关键词：代谢性疾病生长分化因子5 BMP-14 炎症氧化应激

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